ABSTRACT
Alzheimer's Disease (AD) is an age-related neurodegenerative disorder characterized by progressive memory loss and cognitive impairment, affecting 35 million individuals worldwide. Intracerebroventricular (ICV) injection of low to moderate doses of streptozotocin (STZ) in adult male Wistar rats can reproduce classical physiopathological hallmarks of AD. This biological model is known as ICV-STZ. Most studies are focused on the description of behavioral and morphological aspects of the ICV-STZ model. However, knowledge regarding the molecular aspects of the ICV-STZ model is still incipient. Therefore, this work is a first attempt to provide a wide proteome description of the ICV-STZ model based on mass spectrometry (MS). To achieve that, samples from the pre-frontal cortex (PFC) and hippocampus (HPC) of the ICV-STZ model and control (wild-type) were used. Differential protein abundance, pathway, and network analysis were performed based on the protein identification and quantification of the samples. Our analysis revealed dysregulated biological pathways implicated in the early stages of late-onset Alzheimer's disease (LOAD), based on differentially abundant proteins (DAPs). Some of these DAPs had their mRNA expression further investigated through qRT-PCR. Our results shed light on the AD onset and demonstrate the ICV-STZ as a valid model for LOAD proteome description.
Subject(s)
Alzheimer Disease , Rats , Male , Animals , Alzheimer Disease/metabolism , Rats, Wistar , Streptozocin , Proteome , Proteomics , Disease Models, Animal , Maze LearningABSTRACT
O Transtorno de Déficit de Atenção e Hiperatividade (TDAH) se caracteriza pela desatenção, hiperatividade e impulsividade, que podem ser observadas já no início da infância. O TDAH afeta diretamente as Funções Executivas (FE), responsáveis pela regulação de comportamentos sociais e habilidades cognitivas, reduzindo o desempenho escolar e gerando problemas pessoais. Sendo assim, pesquisas relacionadas ao tema mostram-se importantes do aspecto clínico e educacional, podendo contribuir para a melhora da qualidade de vida dos indivíduos afetados. Portanto, neste estudo de revisão sistemática, buscou-se quantificar e explorar artigos com pesquisas empíricas que relacionam o TDAH e as FE. Foram utilizadas as bases de dados: Periódicos CAPES, LILACS, SciELO, PePSIC e SIBiUSP. Os critérios de inclusão foram o ano de publicação (2009-2019), língua portuguesa e artigos relacionados apenas à infância e à adolescência. No total foram encontrados 112 artigos, dos quais seis foram analisados. Os critérios examinados incluíram neuroanatomia, instrumentos de avaliação utilizados para a detecção do transtorno, entrevistas realizadas, gênero e nível escolar dos participantes. Foi observado que os instrumentos mais utilizados, nos artigos analisados, foram o SNAP IV, o WASI, o Teste de Trilhas, o Teste dos Cinco Pontos, o Teste de Atenção por Cancelamento e o subteste Go-no-Go do Neupsilin Infantil. A atual pesquisa permitiu mostrar o que há de mais recente sobre o assunto. Porém, apesar de sua importância, notou-se que existe uma escassez de estudos nacionais que correlacionam estes temas. Logo, são necessários mais estudos empíricos brasileiros que se dediquem a contribuir para estas áreas do conhecimento.
Attention Deficit Hyperactivity Disorder (ADHD) is characterized by inattention, hyperactivity, and impulsivity, which can be observed as early as childhood. The disorder directly affects Executive Functions (FE), those responsible for controlling social performance and cognitive skills, school performance, and the management of personal problems. Thus, research related to the theme shows important clinical and educational aspects and may contribute to improve the quality of life of affected individuals. Therefore, look through this systematic review study, quantify and explore articles with empirical research related to ADHD and EF. CAPES, LILACS, SciELO, PePSIC and SIBiUSP were used as Periodic databases. The inclusion requests were the year of publication (2009-2019), Portuguese language and articles related only to childhood and adolescence. We found 112 articles, of which six were analyzed. The tests examined include neuroanatomy, the assessment tools used to detect disorders, interviews, gender, and school level of the survey participants. It was observed that the most used instruments in the analyzed articles were the SNAP IV, the WASI, the Trail Test, the Five Point Test, the Attention for Cancellation Test and the Go-no-Go subtest Neupsilin childish. The current research shows the latest on the subject. However, despite their importance, there is no shortage of national studies that correlate these themes. Therefore, more Brazilian empirical studies are needed to dedicate the contribution to these areas of knowledge.