Comparative cytogenetic study of a continuous human cell line and its subline susceptible and resistant, respectively, to coxsackie B3 virus.

Cytogenetic characteristics of the J-96 human cell line and its J-41 subline, highly susceptible and resistant, respectively, to coxsackie B3 virus, were compared. The J-41 subline, as compared to the original J-96 line, had fewer chromosomes in the modal class cells (54-57 and 58-62, respectively) mostly at the expense of normal chromosomes. In most J-41 cells chromosome 21 was eliminated and the number of homologues of chromosomes 2, 9, 11, and 12 was reduced. The percentage of marker chromosomes in the J-41 subline (31.3) was slightly higher than in the J-96 line (24.3). The relationship between differences in the karyotypes and properties of the cultures such as resistance to coxsackie B3 virus, capacity to produce virus-induced interferon and to acquire an antiviral state after treatment with interferon were discussed.

[Heterochromatinic segments in chromosomes of cultured human cells, sensitive and resistant to Coxsackie B viruses]. / Geterokhromaticheskie raiony v khromosomakh kul'tur chelovecheskikh kletok, chuvstvitel'nykh i rezistentnykh k virusam Koksaki B.

Comparative karyological studies of C-heterochromatin have been made on line J-96 of human cells, which are susceptible to enteroviruses, and on cell line J-41 derived from this culture and possessing highly specific resistance to Coxsackie B viruses. It was shown that the development of specific resistance to Coxsackie B viruses was accompanied by the loss of one of the chromosomes of pairs 1 and 9, and by the dissapearance of two marker chromosomes. There appeared new marker chromosomes with additional C-heterochromatain regions. The data obtained are discussed with respect to a possible interrelationship between these chromosomal alterations and the specific resistance to Coxsakie B viruses.

[Relationship of the characteristics of the chromosome set in human cell cultures to the production and antiviral action of interferon]. / Sviaz' osobennostei khromosomnogo nabora kul'tur kletok cheloveka s produktsiei i antivirusnym deistviem interferona.

The role of chromosomes 2, 5, 16, and 21 in production and effect of human interferon was checked in human diploid cells, human heteroploid cells J-96 and clone J-41 thereof. The J-41 cells were found to have a lower number of chromosomes 2 as compared to the other cells under study; J-41 cells produce less interferon than the other cells. Most J-41 cells lack chromosome 21. Unlike the other two cell cultures, these cells do not produce antivirus state after treatment with interferon. The number of chromosomes 16 is larger in J-96 cells than in diploid ones, and they are less sensitive to interferon than diploid cells. The experimental results confirm the importance of chromosome 2 for coding for interferon production, other chromosomes taking part in the regulation of this process. The gene of sensitivity to interferon is localized in chromosome 21, the regulator gene coding the production of repressor of sensitivity to interferon is in chromosome 16.

[Clinical features of one of the syndromes associated with oligophrenia--the so-called "elf face"]. / Klinicheskie osobennosti odnogo iz sindromov, sochetaiushchikhsia s oligofreniei,--tak nazyvaemogo sindroma "litsa él'fa"

The authors give a clinical description of 19 patients from 2 years and 10 months-18 years with "elf face" syndrome. The most prominent traits of the disease were a specific look of the face, congenital heart insufficiency, muscular hypotonia, inguinal hernia. The mental state was characterized by an expressed mental retardation with some special traits: relatively well developed speech, talkativeness, good-naturedness, an euphoric mood, inactivity and poor motor functioning. In 3 patients who were older a psychopath-like syndrome was found. A study of the kariotype in 5 cases depicted hetermorphism of homologues in some pairs of chromosomes in more than 10% of the cells as well as an increased amount of structural reconstructions. Indirect calculation have shown that the incidence of the syndrome is approximately equal to 1:25000 births which is significantly more than cited in literature.

[Karyological characteristics of the transplantable human cell line J-96, susceptible to Coxsackie B3 virus, and of its derived subline J-41, specifically resistant to this virus]. / Kariologicheskaia kharakteristika perevivaemoi linii kletok cheloveka J-96, chuvstvitel'noi k virusu Koksaki B3, i poluchennoi iz nee sublinii J-41, spetsificheski rezistentnoi étomu virusu.

The paper is concerned with comparative cytogenetic studies of human cell line J-96 and subline J-41 derived from it. Application of G-, R- and C-techniques for differential staining of chromosomes made it possible to determine both numeric and structural changes in chromosomes of the resistant subline as compared to the initial one. The mechanisms contributing to a decrease in the normal chromosome number and to appearance of new marker chromosomes in the resistant subline cells are discussed as well as the possible relation of the found chromosome changes to the loss of cell sensitivity to the Coxsackie B3 virus.

[Stability of C-band heterochromatin polymorphism in 2 transplantable human cell lines differing in sensitivity to Coxsackie virus B3]. / Stabil'nost' polimorfizma geterokhromatina, vyiavlemogo C-metodom, v dvukh perevivaemykh liniiakh kletok cheloveka, razlichaiushchikhsia po chuvstvitel'nosti k virusu Koksaki B3.

Heterochromatin of chromosomes is studied by means of a C-banding technique for the J-96 line of human cells, which is susceptible to enteroviruses and for the J-41 cell line derived from this culture and possessing high specific resistance to Coxsackie B viruses. The data obtained demonstrate stability of variform C-heterochromatin of chromosome pairs 1, 9 16 and certain marker chromosomes in the course of long-term cultivation.

[Comparative karyologic study of transplantable MIO and MIO-r cells, sensitive and resistant to poliomyelitis virus]. / Sravnitel'noe kariologicheskoe issledovanie perevivaemykh kletok MIO i MIO-r, chuvstvitel'nykh i rezistentnykh k virusu poliomielita.

The continuous MIO cell line, highly sensitive to poliovirus, and the derivative subline MIO-r specifically resistant to this virus have been studied karyologically. It has been shown that these cell lines are different in modal class of chromosomes, mean number of the copies of individual intact chromosomes per cell and in morphology of marker chromosomes.