Simultaneous determination of sulfur compounds from the sulfur pathway in rat plasma by liquid chromatography tandem mass spectrometry: application to the study of the effect of Shao Fu Zhu Yu decoction.

A sensitive, accurate, and time-saving approach was developed for the simultaneous quantification of eight sulfur compounds in the sulfur pathway, which could reflect the status of an organism, including oxidative stress, signal transduction, enzyme reaction, and so on. In order to overcome the instability of highly reactive sulfhydryl compounds, N-ethylmaleimide derivatization was adopted to effectively protect sulfhydryl-containing samples. Using isotope-labeled glutathione (GSH-13C2, 15N), the validated method was demonstrated to offer satisfactory linearity, accuracy, and precision. Separation was done by UHPLC, using a BEH amide column. Accordingly, 0.1% formic acid acetonitrile was selected as the precipitant. A tandem mass spectrometer was coupled to the chromatographic system and afforded a detection limit of 0.2 ng/mL. Good linearity was maintained over a wide concentration range (r2 > 0.994), and the accuracy was in the range of 86.6-114% for all the studied compounds. The precision, expressed in RSD%, ranged from 1.1% to 9.4% as intraday variability and less than 13% as interday precision for all of the analytes. The approach was applied to study the potential therapeutic mechanism of a well-known traditional Chinese medicine, Shao Fu Zhu Yu decoction. The results suggested that Shao Fu Zhu Yu decoction might protect against oxidative damage by increasing the concentrations of sulfhydryl compounds. Graphical abstract An approach to quantitatively determining sulfur compounds in the sulfur pathway simultaneously wasestablished and applied to the study of the effect of Shao Fu Zhu Yu decoction.

Belief in a just world and meaning in life among Chinese adolescents: The mediating role of basic psychological need satisfaction.

Previous literature has shown that adults with higher belief in a just world (BJW) experience greater meaning in life than those with less pronounced BJW. However, the role of BJW in adolescent meaning in life and the associated psychological mechanisms remain unclear. Thus, two studies were conducted to explore the roles of personal belief in a just world (PBJW) and general belief in a just world (GBJW) in meaning in life as well as the explanatory role of basic psychological need satisfaction among Chinese adolescents. Study 1 offered cross-sectional evidence that individuals higher in PBJW and GBJW displayed greater meaning in life, and basic psychological need satisfaction accounted for these associations. Study 2 provided longitudinal evidence that individuals higher in PBJW and GBJW at Time 1 exhibited greater meaning in life six months later (Time 2), wherein basic psychological need satisfaction at Time 1 served as a mediator. These findings contribute to understanding the adaptive benefits of PBJW and GBJW in meaning in life during adolescence and point to a crucial channel connecting BJW and meaning in life.