Malaria diagnostic capacity in health facilities in Ethiopia.

BACKGROUND: Accurate early diagnosis and prompt treatment is one of the key strategies to control and prevent malaria in Ethiopia where both Plasmodium falciparum and Plasmodium vivax are sympatric and require different treatment regimens. Microscopy is the standard for malaria diagnosis at the health centres and hospitals whereas rapid diagnostic tests are used at community-level health posts. The current study was designed to assess malaria microscopy capacity of health facilities in Oromia Regional State and Dire Dawa Administrative City, Ethiopia. METHODS: A descriptive cross-sectional study was conducted from February to April 2011 in 122 health facilities, where health professionals were interviewed using a pre-tested, standardized assessment tool and facilities' laboratory practices were assessed by direct observation. RESULTS: Of the 122 assessed facilities, 104 (85%) were health centres and 18 (15%) were hospitals. Out of 94 health facilities reportedly performing blood films, only 34 (36%) used both thin and thick smears for malaria diagnosis. The quality of stained slides was graded in 66 health facilities as excellent, good and poor quality in 11(17%), 31 (47%) and 24 (36%) respectively. Quality assurance guidelines and malaria microscopy standard operating procedures were found in only 13 (11%) facilities and 12 (10%) had involved in external quality assessment activities, and 32 (26%) had supportive supervision within six months of the survey. Only seven (6%) facilities reported at least one staff's participation in malaria microscopy refresher training during the previous 12 months. Although most facilities, 96 (79%), had binocular microscopes, only eight (7%) had the necessary reagents and supplies to perform malaria microscopy. Treatment guidelines for malaria were available in only 38 (31%) of the surveyed facilities. Febrile patients with negative malaria laboratory test results were managed with artemether-lumefantrine or chloroquine in 51% (53/104) of assessed health facilities. CONCLUSIONS: The current study indicated that most of the health facilities had basic infrastructure and equipment to perform malaria laboratory diagnosis but with significant gaps in continuous laboratory supplies and reagents, and lack of training and supportive supervision. Overcoming these gaps will be critical to ensure that malaria laboratory diagnosis is of high-quality for better patient management.

Proximate composition and selected phytochemical component of Dawrach (Raphanus raphanistrum L.) as affected by blanching temperature.

Dawrach (Raphanus raphanistrum L.) is an underutilized wild edible plant belonging to the Brassicaceae family. Dawrach is widely regarded as a weed, and there is a lack of information about its nutritional profile such as, proximate, antioxidant properties, health-promoting vitamins, minerals and significant phytochemicals. The plant also contains anti-nutrients which need a pretreatment to be minimized and deteriorating enzymes that should be inactivated to make shelf stable products. Blanching temperature is the most critical factor that needs great attention during the blanching process to minimize the anti-nutrients and minimize the loss of desirable components of Dawrach such as, proximate and phytochemical contents and antioxidant activity. Thus, this study aimed to investigate the influence of blanching temperatures on the proximate, phytochemicals and antioxidant activity of Dawrach leaves. The parameters were evaluated following standard analytical methods and procedures. Dawrach leaves were blanched at 50, 60, 70, 80, and 90 °C temperature for 3 min, and raw or unblanched Dawrach was used as a control. Blanching temperature significantly affected the proximate, phytochemicals, antioxidant activity and anti-nutrients of the Dawrach. As a result, the moisture content of raw and blanched at 90 °C was 10.77 and 7.69 g/100g, respectively. Thus, blanching decreases the moisture content of Dawrach to the recommended safe moisture level for the products. Moreover, there was a significant decrease in the values of antioxidants activities, beta carotene, and l-ascorbic acid as the blanching temperature increased from 50 to 90 °C. Besides, as blanching temperature increased, there was a reduction in oxalate and tannin of the Dawrach leaf.

Wild geladas (Theropithecus gelada) in crops-more than in pasture areas-reduce aggression and affiliation.

Human-primate interfaces are expanding and, despite recent studies on primates from peri-urban environments, little research exists on the impact of agriculture and/or pasture areas on primate social behavior and health. We assessed how crop/pasture areas potentially alter social behavior and health of wild geladas (Theropithecus gelada) frequenting the unprotected area of Kundi (Ethiopia). We predicted that compared to pasture areas, crop areas (i) would be more challenging for geladas (prediction 1) and (ii) would have a greater impact on both aggressive and affiliative behavior, by reducing grooming time and enhancing competition (prediction 2). During January-May 2019 and December 2019-February 2020, we collected data (via scan, focal animal sampling, and video analyses) on direct human disturbance, external signs of pathologies and social behavior of 140 individuals from 14 one-male units and two all-male units. Animals experienced the highest level of human disturbance in crop areas (in line with prediction 1). Individuals from the groups preferentially frequenting crop areas showed the highest prevalence of external signs of pathologies consistent with chemical and biological contamination (alopecia/abnormally swollen parts). We collected 48 fecal samples. Samples from frequent crop users contained the highest rates of parasitic elements/gram (egg/larva/oocyst/cyst) from Entamoeba histolytica/dispar, a parasite common in human settlements of the Amhara region. In crop areas, subjects spent less time grooming but engaged in lower rates of intense aggression (in partial agreement with prediction 2). We speculate that the reduction in social behavior may be a tactic adopted by geladas to minimize the likelihood of detection and maximize food intake while foraging in crops.