ABSTRACT
A method of local treatment of wounds to accelerate healing would be a major benefit in those patients in whom abnormal healing is expected. Earlier studies has suggested that local ultrasonic treatment of wounds would stimulate the healing process. We have evaluated the effect of local ultrasound (5 MHz) and thermal treatments on healing in a dermal wound model. Various intensities of ultrasound and heat were employed for 5 min/day (0.05 W/cm2) and 10 min/day (0.05W/cm2), with healing assessed by wound breaking strength measurements obtained 14 days after injury. Subcutaneous temperature measurements demonstrated that equal intensities of ultrasound and heat produced equivalent temperature changes in the tissues. None of the treatments employed resulted in greater wound breaking strengths than the controls, and the higher intensities of ultrasound (0.1 and 0.15 W/cm2) and heat (0.15 W/cm2) resulted in decreased wound breaking strength. Our results failed to support brief daily treatments of local ultrasound or heat as stimulants of wound healing.
Subject(s)
Hot Temperature/therapeutic use , Ultrasonic Therapy , Wound Healing , Animals , Biomechanical Phenomena , Body Temperature , Male , Rats , Rats, Inbred F344 , Skin Physiological Phenomena , Time FactorsSubject(s)
Anaerobiosis , Computers , Metabolism , Monitoring, Physiologic , Exercise Test , Humans , Monitoring, Physiologic/instrumentationSubject(s)
Body Temperature , Hot Temperature/therapeutic use , Equipment and Supplies , Esophagus , Feedback , Humans , Time FactorsABSTRACT
A rapid entry mechanism for an anaerobic glove box is described. The entry port is practical for entry or removal of one or two items and requires only seconds to operate. No deleterious effect on isolation or growth of anaerobes has been encountered since installation of the rapid entry port 3.5 years ago. Details for its construction are given.
Subject(s)
Bacteria/growth & development , Bacteriological Techniques/instrumentation , Environment, Controlled , Anaerobiosis , Microbial Sensitivity TestsABSTRACT
A multibeam optical detection system has been developed with a high optical efficiency, achieved through a reduction in the number of optical interfaces employed in the system. This reduction is made possible by a combination of employing simple lenses, gluing the objective lens directly upon the face of the flow cuvette and the extraction of only one fluorescence signal from each laser beam. A modified flow chamber is also described that includes fluidic resistance elements for the elimination of most of the electric shielding normally associated with electronic cell volume measurements.