High-security space division multiplexing optical transmission scheme based on constellation grid selective twisting.

In this paper, we propose a high-security space division multiplexing optical transmission scheme based on constellation grid selective twisting, which adopts the Rossler chaos model for encrypting PDM-16QAM signals, being applied to a multicore, few-mode multiplexing system. The bitstream of the program is passed through XOR function before performing constellation grid selective twisting and rotation of the constellation map to improve the security of the system. The proposed system is verified experimentally by using 80-wave and 4-mode multiplexing in one of the 19-core 4-mode fibers. Based on the proposed encryption method, a net transmission rate of 34.13 Tbit/s, a transmission distance of 6000 km, and a capacity distance product of 204.8 Pb/s × km is achieved under encrypted PDM-QPSK modulation. Likewise, a net transmission rate of 68.27 Tbit/s, a transmission distance of 1000 km, and a capacity distance product of 68.27 Pb/s × km is achieved based on encrypted PDM-16QAM modulation. It is experimentally verified that the sensitivity of the initial value in Rossler's chaotic model is in the range of 10-16∼10-17. Meanwhile, the proposed encryption scheme achieves a large key space of 10101, which is compatible with the high-capacity distance product multicore and few-mode multiplexing system. It is a promising candidate for the next-generation highly-secured high-capacity transmission system.

K-means non-uniform-quantization digital-analog radio-over-fiber scheme for THz-band photonics-aided wireless fronthaul.

We propose a non-uniform-quantization digital-analog radio-over-fiber (NUQ-DA-RoF) scheme based on an advanced K-means NUQ algorithm and demonstrate it experimentally in a 2-m 300-GHz photonics-aided wireless fronthaul system. Results show that the NUQ-DA-RoF scheme achieves a SNR gain of ∼1.9 dB compared to the uniform-quantization DA-RoF (UQ-DA-RoF) at an equivalent Common Public Radio Interface equivalent data rate (CPRI-EDR). Remarkably, the NUQ-DA-RoF scheme exhibits an ∼1.6-dB power sensitivity enhancement over the UQ-DA-RoF at the 256-QAM SNR threshold. These findings highlight the advantages of the NUQ-DA-RoF scheme over UQ-DA-RoF in terms of power budget and SNR improvement, suggesting promising prospects for future radio access networks and wireless fronthaul.

High-order QAM NANF transmission utilizing MIMO equalizer integrated with low-complexity decision-directed carrier phase estimation.

We experimentally realized a high-speed nested anti-resonant nodeless fiber (NANF) transmission with the assistance of the polarization division multiplexing (PDM) and probabilistic shaping (PS) technology. In this system, a low-complexity multiple-input multiple-output (MIMO) real-valued equalizer (RVE) is integrated with decision-directed carrier phase estimation (DDCPE), which is robust against the IQ cross talk and a tiny phase disturbance between PS symbols. By using the proposed MIMO-RVEDDCPE, the 60-Gbaud PDM-PS-256QAM signal has been delivered through 2-km NANF satisfying the soft-decision forward error correction (SD-FEC) threshold.

Preparation of a Novel Oat ß-Glucan-Chromium(III) Complex and Its Hypoglycemic Effect and Mechanism.

This study synthesized a novel oat ß-glucan (OBG)-Cr(III) complex (OBG-Cr(III)) and explored its structure, inhibitory effects on α-amylase and α-glucosidase, and hypoglycemic activities and mechanism in vitro using an insulin-resistant HepG2 (IR-HepG2) cell model. The Cr(III) content in the complex was found to be 10.87%. The molecular weight of OBG-Cr(III) was determined to be 7.736 × 104 Da with chromium ions binding to the hydroxyl groups of OBG. This binding resulted in the increased asymmetry and altered spatial conformation of the complex along with significant changes in morphology and crystallinity. Our findings demonstrated that OBG-Cr(III) exhibited inhibitory effects on α-amylase and α-glucosidase. Furthermore, OBG-Cr(III) enhanced the insulin sensitivity of IR-HepG2 cells, promoting glucose uptake and metabolism more efficiently than OBG alone. The underlying mechanism of its hypoglycemic effect involved the modulation of the c-Cbl/PI3K/AKT/GLUT4 signaling pathway, as revealed by Western blot analysis. This research not only broadened the applications of OBG but also positioned OBG-Cr(III) as a promising Cr(III) supplement with enhanced hypoglycemic benefits.

Transmission of 4096-QAM OFDM at D-band.

We experimentally demonstrate the transmission of multi-order quadrature amplitude modulation (QAM) orthogonal frequency division multiplexing (OFDM) millimeter wave signals at D-band. Meanwhile, the system nonlinearity analysis is also given, which is originated from the unsatisfactory optoelectronic devices, multi-order QAM and the high peak-to-average power ratio (PAPR) of OFDM signal. To alleviate the system nonlinearity, Volterra nonlinearity compensation (VNC) is adopted. Probabilistic shaping (PS) has been regarded as an effective approach to ensure the system robustness. By using the technologies of probabilistic shaping and Volterra nonlinearity compensation, 57.21-Gbit/s 4096-QAM OFDM signal at 117 GHz can be delivered over 13.42-m wireless distance in our experiment, achieving the normalized general mutual information (NGMI) threshold of 0.83 with 25% soft-decision forward-error correction (SD-FEC) overhead. In addition, we simulated the D-band millimeter wave simulation system in VPI software. The NGMI performance between conventional and discrete Fourier transform-spread (DFT-S) 16-QAM OFDM has been compared in simulation at the same optical signal-to-noise ratio (OSNR). The case of conventional 16-QAM OFDM has better performance. To the best of our knowledge, this is the first demonstration of transmission of multi-order QAM OFDM millimeter wave signal at D-band using PS and VNC methods.

Real-time demonstration of a low-complexity PS scheme for 16QAM-DMT signals in an IM-DD system.

We experimentally demonstrated a low-complexity probabilistic shaping (PS) 16-ary quadrature amplitude modulation (16QAM) scheme based on intra-symbol bit-weighted distribution matching (Intra-SBWDM) for discrete multi-tone (DMT) symbols with field-programmable gate array implementation in an intensity modulation and direct detection (IM-DD) system. Different from the traditional PS scheme such as Gallager many-to-one mapping, hierarchical distribution matching and constant composition distribution matching, the Intra-SBWDM scheme with lower computation and hardware complexity does not need to continuously refine the interval to find the target symbol probability, nor does it need a look-up table, so it does not introduce a large number of extra redundant bits. In our experiment, four PS parameter values (k = 4, 5, 6, and 7) are investigated in a real-time short-reach IM-DD system. 31.87-Gbit/s net bit PS-16QAM-DMT (k = 4) signal transmission is achieved. The results show that the receiver sensitivity in terms of the received optical power of the real-time PS scheme based on Intra-SBWDM (k = 4) over OBTB/20 km standard single-mode fiber can be improved by about 1.8/2.2 dB at the bit error rate (BER) of 3.8 × 10-3, compared to the uniformly-distributed DMT. In addition, the BER is steadily lower than 3.8 × 10-3 during a one-hour measurement for PS-DMT transmission system.

FPGA implementation for 44.2368-Gbit/s PAM8 signal transmission with pruned pre-equalization.

In this experiment, we demonstrated an intensity modulation and direct-detection (IM/DD) system based on a field-programmable gate array (FPGA). The PAM8 signals are successfully delivered at 44.2368 Gbit/s over a 45-km standard single-mode fiber (SSMF), satisfying the soft-decision forward error correction (SD-FEC) criterion of 2.4 × 10-2, and the net bit rate may reach 36.864 Gbit/s without the need of optical amplifiers. At the transmitter, we used a pruned pre-equalization algorithm to process the PAM8 signals, and the high-speed parallel PAM8 signals were processed at the receiver using 64 parallel constant modulus algorithm (CMA) and decision-directed least mean square (DD-LMS) equalizers. Additionally, we analyzed the bit error rate (BER) performance of the DD-LMS equalizer in FPGA simulation with various data lengths, both before and after equalization.

Probabilistic shaping-based delta sigma modulation.

This Letter proposes a probabilistic shaping delta-sigma modulation technique. By performing delta-sigma modulation on the probability shaping signal at the transmitting end, under the same transmit power and the same net bit rate, the delta-sigma modulation signal based on probability shaping can obtain better anti-noise capability than the delta-sigma modulation signal only. Under the same information entropy conditions, the bit error rate performance of the PS-based 131072QAM signal modulated by delta-sigma modulation is better than that of the ordinary 65536QAM signal using the delta-sigma modulation scheme, and lower than that of soft decision-forward error correction (4 × 10-2).

FPGA-based 4 × 29.4912 Gbit/s PS-PAM4 signal transmission with a low-complexity probabilistic shaping scheme.

In this experiment, we demonstrate a real-time intensity modulation and direct detection (IM/DD) system based on a field programmable gate array (FPGA). For high-speed parallel signal processing, we propose and implement the simplified parallel-constant modulus algorithm (CMA) and decision-directed least mean square (DDLMS) equalizers with low complexity and low latency. Moreover, the bit-class probabilistic shaping (PS) scheme is adopted with very few hardware resources. The digital signal processing (DSP) steps are implemented in the XCVU9P-FLGB2104-2-I Xilinx FPGA with a clock frequency of 230.4 MHz. Based on the experimental results, 4 × 29.4912 Gbit/s PS-pulse amplitude modulation (PAM4) signals can be successfully transmitted over 25 km of standard single-mode fiber (SSMF) while satisfying the hard-decision forward error correction (HD-FEC) threshold at 3.8 × 10-3. Compared with the uniformly distributed PAM4 signal, the low-complexity PS scheme can improve the receiver sensitivity by more than 1 dB.

65,536-QAM OFDM signal transmission over a fiber-THz system at 320†GHz with delta-sigma modulation.

The transmission of a 65,536-ary quadrature amplitude modulation (QAM) orthogonal frequency division multiplexing (OFDM) signal supported by a hybrid fiber-terahertz (THz) multiple-input multiple-output (MIMO) system at 320 GHz is experimentally demonstrated in this Letter. We adopt the polarization division multiplexing (PDM) technique to double the spectral efficiency. Based on a 23-GBaud 16-QAM link, 2-bit delta-sigma modulation (DSM) quantization enables 65,536-QAM OFDM signal transmission over a 20-km standard single-mode fiber (SSMF) and a 3-m 2 × 2 MIMO wireless delivery, and satisfies the hard-decision forward error correction (HD-FEC) threshold of 3.8 × 10-3, corresponding to a net rate of 60.5 Gbit/s for THz-over-fiber transport. Meanwhile, below the fronthaul error vector magnitude (EVM) threshold of 0.34%, a maximum signal-to-noise ratio (SNR) of 52.6 dB is achieved. To the best of our knowledge, this is the highest modulation order achievable for DSM applications in THz communication.

PDM-OFDM 1-bit DSM 1024QAM/4096QAM signals at W-band transmission over 4.6-km wireless distance.

In this Letter, we propose the application of delta-sigma modulation (DSM) higher-order quadrature amplitude modulation (QAM) technology in long-distance transmission of W-band wireless communication, and demonstrate, for the first time to the best of our knowledge, the wireless transmission of millimeter wave signals in the W-band based on 1-bit DSM quantization using polarization-division-multiplexed orthogonal frequency division multiplexing (PDM-OFDM) 1024QAM/4096QAM for 4.6 km. We successfully achieved a bit error rate (BER) of 40-Gbit/s PDM-OFDM 1024QAM and 48-Gbit/s PDM-OFDM 4096QAM after 4.6-km wireless transmission, both lower than the soft decision forward error correction (SD-FEC) of 4.2 × 10-2. To the best of our knowledge, this is the first time that up to 4096QAM signals have been quantized and transmitted based on 1-bit DSM in a 4.6-km-long distance W-band millimeter wave system.

Beyond 300-Gbps/λ photonics-aided THz-over-fiber transmission employing MIMO single-carrier frequency-domain equalizer.

We experimentally realized a 320-GHz 320-Gbps/λ terahertz (THz) radio-over-fiber (RoF) system based on a photonics-aided scheme with the help of polarization-division multiplexing (PDM) technology and multiple-input, multiple-output (MIMO) transmission. In this system, the low-complexity MIMO single-carrier frequency-domain equalizer (SCFDE) is implemented to compensate for the polarization-related impairments of the PDM signal, and the demultiplexing performances between SCFDE and the commonly used constant modulus algorithm (CMA) are also compared in this proposed system. After 20-km standard single-mode fiber (SSMF) and 3-m 2 × 2 MIMO wireless link transmission, the bit error rate (BER) of the received 46-GBaud PDM 16-ary quadrature amplitude modulation (16QAM) signal satisfies the soft-decision forward error correction (SD-FEC) threshold with 15% overhead, which corresponds to a record-breaking net bit rate of 320 Gbit/s.

SNR improved digital-delta-sigma-modulation radio-over-fiber scheme for D-band 4.6-km photonics-aided wireless fronthaul.

We propose a digital-delta-sigma-modulation radio-over-fiber (DDSM-RoF) scheme for wireless fronthaul and validate it experimentally in a D-band photonics-aided RoF transmission system. The 10-Gbaud DDSM-RoF signal with a common public radio interface equivalent data rate (CPRI-EDR) of 55.8 Gb/s is successfully transmitted in a 130-GHz 4.6-km wireless channel. The spectral efficiency (SE) is 5.58 bit/s/Hz and the capacity-distance product reaches 257 Gb/s·km. Up to 34.4-dB recovered signal-to-noise ratio (SNR) is observed to support the 1024-quadrature-amplitude-modulation (1024-QAM) transmission. Compared with the digital-analog-RoF (DA-RoF) scheme, the proposed DDSM-RoF achieves an SNR improvement of 5.9 dB.

SNR-enhanced signal delivery scheme with delta-sigma modulation in a four-mode fiber system.

This Letter proposes a scheme for optimizing the signal-to-noise ratio (SNR) of signal to improve the system performance by a 1 bit delta-sigma modulation (DSM) in a four-mode MDM system for mobile fronthaul. A 1 bit digitalized signal with an SNR of 60 dB from transmitter digital signal processing (Tx DSP) can be achieved. Based on this system, an experimental demonstration of the ultrahigh-order 1048576-QAM signal transmission over a 50 km strong-coupling few-mode fiber (FMF) is successfully realized. With DSP, the bit error rate (BER) of the received 1048576-QAM signals over four modes transmission is below the 20% soft-decision forward error correction (20% SD-FEC) threshold of 2.4 × 10-2. To the best of our knowledge, this is the first time that the combination of DSM technology and strong-coupling MDM system is achieved and that the highest-modulation order with DSM reported in MDM system is reached. This experimental demonstration of the proposed novel scheme in MDM system can provide an effective solution for ultra-large-capacity mobile fronthaul in the future.

THz-over-fiber transmission with a net rate of 5.12 Tbps in an 80 channel WDM system.

An experimental demonstration of the ultra-large-capacity terahertz (THz)-wave signal transmission over a 20-km wired and 54-m wireless distance in an 80-channel wavelength division multiplexing (WDM) system is successfully realized for the first time, to the best of our knowledge. Based on optical asymmetric single-sideband (ASSB) modulation and advanced digital signal processing (DSP) algorithms, we achieve a record line rate of 6.4 Tbit/s (net rate of 5.12 Tbit/s) in a THz-over-fiber communication system. This experimental demonstration can provide an effective solution for ultra-large-capacity THz-over-fiber communication in future 6G networks.

Mitigation of crosstalk between RSB and LSB signals generated by one I/Q modulator.

Optical independent sideband (ISB) signals can be generated by exploiting one external In-phase/Quadrature (I/Q) modulator. Our theoretical analysis shows crosstalk between the two ISB (right and left side) signals can attribute to two main imperfections: amplitude difference and phase unmatched in I/Q data. To reduce the impact of crosstalk between the two ISB signals, we propose three schemes. The first is precise phase match of the I and Q data. The second has been made possible by setting different frequencies for the left sideband (LSB) and the right sideband (RSB) signals, and the last is achieved by adding Multiple-Input Multiple-Output (MIMO) equalization digital signal processing (DSP) at the receiver side. Our experimental results have shown that these schemes can improve the performance of ISB signals. In our experimental system we designed dual ISB system with different modulation formats in two sidebands. Precise phase match can bring a ∼2.2dB improvement at BER of 1×10-2 and a ∼4.3dB improvement at BER of 1×10-3 for 16-ary quadrature-amplitude-modulation (16QAM) and quadrature-phase-shift-keying (QPSK) signals, respectively, in 4Gbaud with carrier frequency of 36GHz system. The BER of 4Gbaud 16QAM ISB signal at 30GHz and 4Gbaud QPSK ISB signal at 38GHz can reach hard-decision forward-error-correction (HD-FEC) when the input power is larger than -5.5 and -7.4dBm respectively in different frequencies system. For 4Gbaud with carrier frequency of 36GHz system, the BER of 16QAM signal and QPSK signal reduce ∼2.1 and ∼2.2dB at HD-FEC after using MIMO. In addition, MIMO can further improve the performance of the matched phase system or the system with different frequencies.

Independent dual single-sideband vector millimeter-wave signal generation by one single I/Q modulator.

We propose a new scheme to realize the independent dual single-sideband (SSB) vector millimeter-wave (mm-wave) signal generation based on one single in-phase/quadrature (I/Q) modulator. The two SSB vector mm-wave signals can have independent carrier frequencies and modulation formats. We experimentally demonstrate the simultaneous generation and transmission of 38 GHz 16-ary quadrature-amplitude-modulation (16QAM) and 40 GHz quadrature-phase-shift-keying (QPSK) mm-wave signals based on this scheme. The penalty of the 16QAM and QPSK mm-wave signals after transmission over 10-km standard single-mode fiber and 0.5-m wireless link can be neglected.

100 Gbit/s VSB-PAM-n IM/DD transmission system based on 10 GHz DML with optical filtering and joint nonlinear equalization.

We experimentally demonstrate the transmission of 100 Gbit/s and beyond vestigial sideband (VSB) n-level pulse amplitude modulation (PAM-n) signals, with a commercial 10 GHz-class directly modulated laser (DML) in the C-band, using optical filtering. To mitigate transmission impairments at the transmitter side, Nyquist pulse shaping and Kaiser Window filtering techniques are used to overcome the limited bandwidth of optoelectronic devices. At the receiver side, the joint nonlinear equalization based on cascaded multi-modulus algorithm (CMMA) and Volterra Filter (VF) is used to reduce the strong nonlinear impairments from chirp and chromatic dispersion (CD). 100 Gb/s PAM-4, 107.5 Gb/s PAM-4, and 101.25 Gb/s PAM-8 signals can be successfully transmitted over 45 km, 10 km and 10 km standard single-mode fiber (SSMF) under the bit-error-ratio (BER) of 3.8 × 10-3, respectively.

Physical mapping and candidate gene prediction of fertility restorer gene of cytoplasmic male sterility in cotton.

BACKGROUND: Cytoplasmic male sterility (CMS) is a maternally inherited trait failing to produce functional pollen. It plays a pivotal role in the exploitation of crop heterosis. The specific locus amplified fragment sequencing (SLAF-seq) as a high-resolution strategy for the identification of new SNPs on a large-scale is gradually applied for functional gene mining. The current study combined the bulked segregant analysis (BSA) with SLAF-seq to identify the candidate genes associated with fertility restorer gene (Rf) in CMS cotton. METHODS: Illumina sequencing systematically investigated the parents. A segregating population comprising of 30 + 30 F2 individuals was developed using 3096A (female parent) as sterile and 866R (male parent) as a restorer. The original data obtained by dual-index sequencing were analyzed to obtain the reads of each sample that were compared to the reference genome in order to identify the SLAF tag with a polymorphism in parent lines and the SNP with read-associated coverage. Based on SLAF tags, SNP-index analysis, Euclidean distance (ED) correlation analysis, and whole genome resequencing, the hot regions were annotated. RESULTS: A total of 165,007 high-quality SLAF tags, with an average depth of 47.90× in the parents and 50.78× in F2 individuals, were sequenced. In addition, a total of 137,741 SNPs were detected: 113,311 and 98,861 SNPs in the male and female parent, respectively. A correlation analysis by SNP-index and ED initially located the candidate gene on 1.35 Mb of chrD05, and 20 candidate genes were identified. These genes were involved in genetic variations, single base mutations, insertions, and deletions. Moreover, 42 InDel markers of the whole genome resequencing were also detected. CONCLUSIONS: In this study, associated markers identified by super-BSA could accelerate the study of CMS in cotton, and as well as in other crops. Some of the 20 genes' preliminary characteristics provided useful information for further studies on CMS crops.

Hepatitis C virus-induced up-regulation of microRNA-155 promotes hepatocarcinogenesis by activating Wnt signaling.

UNLABELLED: Hepatitis C virus (HCV) infection usually induces chronic hepatic inflammation, which favors the initiation and progression of hepatocellular carcinoma (HCC). Moreover, microRNA-155 (miR-155) plays an important role in regulating both inflammation and tumorigenesis. However, little is known about whether and how miR-155 provides the link between inflammation and cancer. In this study we found that miR-155 levels were markedly increased in patients infected with HCV. MiR-155 transcription was regulated by nuclear factor kappa B (NF-κB), and p300 increased NF-κB-dependent miR-155 expression. The overexpression of miR-155 significantly inhibited hepatocyte apoptosis and promoted cell proliferation, whereas miR-155 inhibition induced G(0) /G(1) arrest. Up-regulated miR-155 resulted in nuclear accumulation of ß-catenin and a concomitant increase in cyclin D1, c-myc, and survivin. Gain-of-function and loss-of-function studies demonstrated that miR-155 promoted hepatocyte proliferation and tumorigenesis by increasing Wnt signaling in vitro and in vivo, and DKK1 (Wnt pathway inhibitor) overexpression inhibited the biological role of miR-155 in hepatocytes. Finally, adenomatous polyposis coli (APC), which negatively regulates Wnt signaling, was identified as the direct and functional target of miR-155. CONCLUSION: HCV-induced miR-155 expression promotes hepatocyte proliferation and tumorigenesis by activating Wnt signaling. The present study provides a better understanding of the relationship between inflammation and tumorigenesis, and thus may be helpful in the development of effective diagnosis and treatment strategies against HCV-HCC.