ABSTRACT
Hereditary neuralgic amyotrophy (HNA) is an autosomal dominant recurrent neuropathy affecting the brachial plexus. HNA is triggered by environmental factors such as infection or parturition. We report three mutations in the gene septin 9 (SEPT9) in six families with HNA linked to chromosome 17q25. HNA is the first monogenetic disease caused by mutations in a gene of the septin family. Septins are implicated in formation of the cytoskeleton, cell division and tumorigenesis.
Subject(s)
Brachial Plexus Neuritis/genetics , Chromosomes, Human, Pair 17/genetics , GTP Phosphohydrolases/genetics , Mutation , Amino Acid Sequence , Animals , Base Sequence , Dogs , Humans , Mice , Molecular Sequence Data , Rats , SeptinsABSTRACT
Inclusion body myopathy associated with Paget disease of bone and frontotemporal dementia (IBMPFD) is a dominant progressive disorder that maps to chromosome 9p21.1-p12. We investigated 13 families with IBMPFD linked to chromosome 9 using a candidate-gene approach. We found six missense mutations in the gene encoding valosin-containing protein (VCP, a member of the AAA-ATPase superfamily) exclusively in all 61 affected individuals. Haplotype analysis indicated that descent from two founders in two separate North American kindreds accounted for IBMPFD in approximately 50% of affected families. VCP is associated with a variety of cellular activities, including cell cycle control, membrane fusion and the ubiquitin-proteasome degradation pathway. Identification of VCP as causing IBMPFD has important implications for other inclusion-body diseases, including myopathies, dementias and Paget disease of bone (PDB), as it may define a new common pathological ubiquitin-based pathway.
Subject(s)
Cell Cycle Proteins/physiology , Muscular Diseases/genetics , Mutation , Osteitis Deformans/genetics , Adenosine Triphosphatases , Cell Cycle Proteins/genetics , Chromosome Mapping , Chromosomes, Human, Pair 9 , Female , Humans , Immunohistochemistry , Male , Muscular Diseases/physiopathology , Osteitis Deformans/physiopathology , Pedigree , Valosin Containing ProteinABSTRACT
Inclusion body myopathy with Paget disease of the bone (PDB) and/or frontotemporal dementia (IBMPFD, OMIM 167320), is a progressive autosomal dominant disorder caused by mutations in the Valousin-containing protein (VCP, p97 or CDC48) gene. IBMPFD can be difficult to diagnose. We assembled data on a large set of families to illustrate the number and type of misdiagnoses that occurred. Clinical analysis of 49 affected individuals in nine families indicated that 42 (87%) of individuals had muscle disease. The majority were erroneously diagnosed with limb girdle muscular dystrophy (LGMD), facioscapular muscular dystrophy, peroneal muscular dystrophy, late adult onset distal myopathy, spinal muscular atrophy, scapuloperoneal muscular dystrophy, or amyotrophic lateral sclerosis (ALS) among others. Muscle biopsies showed rimmed vacuoles characteristic of an inclusion body myopathy in 7 of 18 patients (39%), however, inclusion body myopathy was correctly diagnosed among individuals in only families 5 and 15. Frontotemporal dementia (FTD) was diagnosed in 13 individuals (27%) at a mean age of 57 years (range 48.9-60.2 years); however, several individuals had been diagnosed with Alzheimer disease. Histopathological examination of brains of three affected individuals revealed a pattern of ubiquitin positive neuronal intranuclear inclusions and dystrophic neurites. These families expand the clinical phenotype in IBMPFD, a complex disorder caused by mutations in VCP. The presence of PDB in 28 (57%) individuals suggests that measuring serum alkaline phosphatase (ALP) activity may be a useful screen for IBMPFD in patients with myopathy.
Subject(s)
Adenosine Triphosphatases/genetics , Cell Cycle Proteins/genetics , Dementia/complications , Dementia/genetics , Family , Muscular Diseases/complications , Muscular Diseases/genetics , Osteitis Deformans/complications , Osteitis Deformans/genetics , Adult , Cross-Sectional Studies , Female , Humans , Inclusion Bodies/ultrastructure , Male , Middle Aged , Muscle, Skeletal/pathology , Muscle, Skeletal/ultrastructure , Pedigree , Retrospective Studies , Valosin Containing ProteinABSTRACT
Frontotemporal dementia (FTD) with inclusion body myopathy and Paget disease of bone (IBMPFD) is a rare, autosomal-dominant disorder caused by mutations in the valosin-containing protein (VCP) gene, a member of the AAA-ATPase gene superfamily. The neuropathology associated with sporadic FTD is heterogeneous and includes tauopathies and frontotemporal lobar degeneration with ubiquitin-positive inclusions (FTLD-U). However, there is limited information on the neuropathology in IBMPFD. We performed a detailed, systematic analysis of the neuropathologic changes in 8 patients with VCP mutations. A novel pattern of ubiquitin pathology was identified in IBMPFD that was distinct from sporadic and familial FTLD-U without VCP gene mutations. This was characterized by ubiquitin-positive neuronal intranuclear inclusions and dystrophic neurites. In contrast to FTLD-U, only rare intracytoplasmic inclusions were identified. The ubiquitin pathology was abundant in the neocortex, less robust in limbic and subcortical nuclei, and absent in the dentate gyrus. Only rare inclusions were detected with antibodies to VCP and there was no biochemical alteration in the VCP protein. VCP is associated with a variety of cellular activities, including regulation of the ubiquitin-proteasome system. Our findings are consistent with the hypothesis that the pathology associated with VCP gene mutations is the result of impairment of ubiquitin-based degradation pathways.
Subject(s)
Cell Cycle Proteins/genetics , Dementia/genetics , Dementia/metabolism , Mutation/genetics , Ubiquitin/metabolism , Adenosine Triphosphatases , Blotting, Western/methods , Dementia/pathology , Female , Humans , Immunohistochemistry/methods , Male , Myositis, Inclusion Body/genetics , Myositis, Inclusion Body/pathology , Osteitis Deformans/genetics , Osteitis Deformans/pathology , Valosin Containing ProteinABSTRACT
Paget's disease of bone (PDB) is a common metabolic bone disease of late onset with a strong genetic component. Rarely, PDB can occur as part of a syndrome in which the disease is accompanied by inclusion body myopathy and frontotemporal dementia (inclusion body myopathy, Paget's disease and frontotemporal dementia, IBMPFD). Recently, IBMPFD has been shown to be caused by mutations in Valosin-containing Protein (VCP), which is required for the proteasomal degradation of phosphorylated IkappaB-alpha, a necessary step in the activation of the transcription factor NF-kappaB. Here, we evaluated the role of VCP in the pathogenesis of typical PDB. We conducted mutation screening of VCP in 44 kindreds with familial Paget's disease recruited mainly through clinic referrals in the UK, Australia and New Zealand. We also performed an association study of VCP haplotypes in patients with PDB who did not have a family history of the disease (sporadic PDB). No mutations were found in VCP in three PDB families where there was evidence of allele sharing between affected subjects in the VCP critical region on chromosome 9p13. We failed to detect disease-associated mutations in any of the three exons previously reported to contain IBMPFD mutations in a further 41 PDB families. We found no evidence of allelic association between common VCP haplotypes in a case-control study of 179 sporadic PDB patients and 172 age- and sex-matched controls. Genetic variation in VCP does not appear to be a common cause of familial or sporadic PDB in the absence of myopathy and dementia.
Subject(s)
Cell Cycle Proteins/genetics , Genetic Predisposition to Disease , Haplotypes , Osteitis Deformans/genetics , Adenosine Triphosphatases , Alleles , Case-Control Studies , DNA Mutational Analysis , Databases, Nucleic Acid , Female , Gene Frequency , Genetic Testing , Humans , Male , Mutation , Osteitis Deformans/etiology , Pedigree , Polymorphism, Single Nucleotide , Valosin Containing ProteinABSTRACT
We have previously reported a new autosomal dominant inclusion body myopathy clinically resembling limb girdle muscular dystrophy, associated with Paget disease of bone in the majority and frontotemporal dementia in a third of individuals. The critical locus for this unique disorder now termed IBMPFD is 9 p21.1-p12, spans 5.5 Mb and contains the gene responsible for the recessive quadriceps-sparing inclusion body myopathy (IBM2). Mutation analysis of the GNE gene associated with IBM2 in affected individuals from four IBMPFD families did not identify any mutations, indicating that the two disorders are not allelic. Expression studies indicate that GNE has a tissue-specific splice pattern, with four splice variants. Mutation analysis in three other candidate genes (beta-tropomyosin, NDUFB6 and SMU1) did not identify any mutations.
Subject(s)
Caenorhabditis elegans Proteins , Carbohydrate Epimerases/genetics , Carrier Proteins/genetics , Chromosomes, Human, Pair 9 , Genetic Heterogeneity , Myositis, Inclusion Body/genetics , Phosphotransferases (Alcohol Group Acceptor)/genetics , Adolescent , Adult , Aged , Amino Acid Sequence , Base Sequence , DNA Mutational Analysis/methods , DNA, Recombinant , Dementia/complications , Dementia/genetics , Exons , Genetic Linkage , Genetic Markers , Humans , Middle Aged , Molecular Sequence Data , Muscular Dystrophies/complications , Muscular Dystrophies/genetics , Mutation , Myositis, Inclusion Body/complications , NADH, NADPH Oxidoreductases/genetics , Nuclear Proteins/genetics , Osteitis Deformans/complications , Osteitis Deformans/genetics , Pedigree , RNA, Messenger/biosynthesis , Reverse Transcriptase Polymerase Chain Reaction/methods , Tropomyosin/geneticsABSTRACT
PURPOSE: Inclusion-body myopathy, Paget's disease of bone and frontotemporal dementia is an adult-onset autosomal dominant illness (IBMPFD) caused by mutations in the valosin-containing protein (VCP) on chromosome 9p21.1-p12. The penetrance of the gene is 82% for myopathy, 49% for Paget's disease, but may be as low as 30% for frontotemporal dementia. Modifier genes could account for decreased frontotemporal dementia penetrance. In this study apolipoprotein-E (APOE) was evaluated for this role in IBMPFD families based on its known modifier effect in Alzheimer's disease. METHODS: From a database of 231 members of 15 families, 174 had APOE genotype available for analysis. Logistic regressions on APOE genotype and frontotemporal dementia were performed, using appropriate covariates. RESULTS AND CONCLUSION: FTD was associated with APOE 4 genotype (P=0.0002), myopathy (P=0.0006), and age (P=0.01), but not microtubule associated protein tau (MAPT) H2 haplotype (P=0.5) or gender (0.09) after adjustment for membership in pedigrees with at least one APOE 4 genotype. These data suggest a potential link between APOE 4 genotype and the specific form of frontotemporal dementia found in IBMPFD. The molecular basis of this link bears further investigation. We did not observe an association of frontotemporal dementia and H2 MAPT haplotype.
Subject(s)
Apolipoproteins E/genetics , Dementia/genetics , Genes, Dominant , Haplotypes/genetics , Adult , Female , Genetic Predisposition to Disease/genetics , Genotype , Humans , Male , Middle Aged , Muscular Diseases/genetics , tau Proteins/geneticsABSTRACT
We report on an unusual family with an autosomal dominant limb-girdle type of myopathy and bone fragility. This family was previously reported by Henry et al. [1958] as autosomal dominant progressive limb girdle "muscular dystrophy" with propensity to fractures and defective healing of long bones. Clinical, biochemical, and radiological aspects were evaluated in eight living relatives in this family (three males and five females) and in eight deceased individuals. The average age-of-onset of the limb-girdle myopathy was 31 years occurring in 87% of affected individuals. The average age of onset of fractures was 24 years occurring in 88% of affected individuals. Biochemical analysis showed a mean alkaline phosphatase (ALP) of 64 U/L (normal 30-120) and borderline high creatine kinase (CK) of 213 U/L (normal 4-220). Radiographs revealed coarse trabeculation, patchy sclerosis, cortical thickening, and narrowing of the medullary cavity with an appearance not considered typical of Paget disease of bone (PDB) or of fibrous dysplasia. Results of nerve conduction studies were normal, and electromyograms and muscle biopsies documented non-specific myopathic changes. There is premature graying with thin hair, thin skin, hernias and the affected individuals appear older than their chronological age, and three members had a clotting disorder. Linkage analysis for markers for the chromosome 9p22.3-q12 locus indicated that the disorder in this family does not segregate with markers in the critical region of limb-girdle/inclusion body myopathy, PDB, and frontotemporal dementia (FTD) [IBMPFD, OMIM #605382]. Sequencing of Valosin-containing protein (VCP), the gene associated with IBMPFD, did not identify mutations. We have excluded linkage to the known loci for limb-girdle type of myopathy and bone disease and excluded several candidate genes. Elucidation of the novel molecular basis of this disorder may provide valuable links between bone, collagen and muscle, and targeted therapeutic options.
Subject(s)
Fractures, Bone/diagnosis , Genes, Dominant , Muscular Dystrophies, Limb-Girdle/diagnosis , Adult , Age of Onset , Aged , Alkaline Phosphatase/metabolism , Chromosomes, Human, Pair 9/genetics , Creatine Kinase/metabolism , Female , Fractures, Bone/genetics , Genetic Linkage , Genetic Markers , Genetic Predisposition to Disease , Humans , Male , Microsatellite Repeats , Middle Aged , Muscular Dystrophies, Limb-Girdle/genetics , Mutation/genetics , PedigreeABSTRACT
Autosomal dominant proximal limb girdle or inclusion body myopathy, associated with Paget disease of bone and frontotemporal dementia (IBMPFD) is a recently described disorder that maps to chromosome 9p21.1-p12. We refined the critical locus and identified the gene as the Valosin Containing Protein (VCP) gene, a member of the AAA-ATPase superfamily using a candidate gene approach. Six missense mutations were found to co-segregate with affected individuals only, two of these representing mutation hot spots. We report the clinical and molecular findings in 99 individuals in 13 families. VCP is associated with a variety of cellular activities, including the control of cell cycle, membrane fusion, and the ubiquitin-proteasome degradation pathway. Previous studies have associated VCP mutants in cell lines with vacuole formation and aggregate formation. Identification of VCP as the gene causing IBMPFD has important implications for understanding the pathogenesis of neurodegenerative disorders.
Subject(s)
Cell Cycle Proteins/genetics , Mutation, Missense/genetics , Myositis, Inclusion Body/genetics , Osteitis Deformans/genetics , Pick Disease of the Brain/genetics , Adenosine Triphosphatases , Adolescent , Adult , Child , Child, Preschool , Chromosome Mapping , Chromosomes, Human, Pair 9/genetics , Female , Humans , Male , Middle Aged , Myositis, Inclusion Body/pathology , Osteitis Deformans/pathology , Pick Disease of the Brain/pathology , Valosin Containing ProteinABSTRACT
Mutations in the valosin-containing protein (VCP) gene on chromosome 9p13-p12 recently have been shown to cause autosomal dominant inclusion body myopathy associated with Paget's disease of the bone and frontotemporal dementia. Here, we report the central nervous system autopsy findings in a 55-year-old German patient with inclusion body myopathy and frontotemporal dementia who harbors a heterozygous R155C missense mutation residing in the N-terminal CDC48 domain of VCP, which is involved in ubiquitin binding. We demonstrate that mutant VCP causes a novel type of frontotemporal dementia characterized by neuronal nuclear inclusions containing ubiquitin and VCP.
Subject(s)
Cell Cycle Proteins/genetics , Dementia/genetics , Genetic Predisposition to Disease , Mutation, Missense , Adenosine Triphosphatases , Amyloid beta-Protein Precursor/metabolism , Arginine/genetics , Blotting, Western/methods , Brain/metabolism , Brain/pathology , Cell Cycle Proteins/metabolism , Cysteine/genetics , DNA Mutational Analysis/methods , Dementia/metabolism , Dementia/physiopathology , Female , Glial Fibrillary Acidic Protein/metabolism , Humans , Immunohistochemistry/methods , Middle Aged , Staining and Labeling/methods , Ubiquitin/metabolism , Valosin Containing Protein , tau Proteins/metabolismABSTRACT
Progressive myopathy of a limb-girdle distribution and bone fragility is a rare autosomal dominant disorder of unknown etiology. Affected individuals, within this family, present with various combinations of progressive muscle weakness, easy fracturing, and poor healing of long bones. Additional features include premature graying with thin hair, thin skin, hernias, and clotting disorders. Electromyograms show myopathic changes and biopsies reveal non-specific myopathic changes. Skeletal radiographs demonstrate coarse trabeculation, patchy sclerosis, cortical thickening, and narrowing of medullary cavities. We report genetic mapping of this disorder to chromosome 9p21-p22 in a multigenerational family. A genome-wide scan for the disease locus obtained a maximal LOD score of 3.74 for marker GATA87E02 N (D9S1121). Haplotype analysis localized the disease gene within a 15 Mb interval flanked by markers AGAT142P and GATA5E06P. This region also localizes diaphyseal medullary stenosis with malignant fibrous histiocytoma (DMS-MFH). Identification of the disease gene will be necessary to understand the pathogenesis of this complex disorder.
Subject(s)
Chromosome Mapping , Chromosomes, Human, Pair 9 , Fractures, Bone/genetics , Muscular Dystrophies, Limb-Girdle/genetics , Muscular Dystrophies, Limb-Girdle/pathology , Adolescent , Adult , Aged , Female , Fractures, Bone/etiology , Genetic Markers , Haplotypes , Humans , Inheritance Patterns , Male , Middle Aged , Muscular Dystrophies, Limb-Girdle/physiopathology , PedigreeABSTRACT
Hereditary neuralgic amyotrophy (HNA) is an autosomal dominant disorder that is associated with episodic recurrent brachial plexus neuropathy. A mutation for HNA maps to chromosome 17q25. To refine the HNA locus further, we carried out genetic linkage studies in seven pedigrees with a high density set of DNA markers from chromosome 17q25. All pedigrees demonstrated linkage to chromosome 17q25, and an analysis of recombinant events placed the HNA locus within an interval of approximately 1 Mb flanked by markers D17S722 and D17S802. In order to test the power of linkage disequilibrium mapping, we compared genotypes of 12 markers from seven pedigrees that were from the United States and that showed linkage to chromosome 17q25. The haplotypes identified a founder effect in six of the seven pedigrees with a minimal shared haplotype that further refines the HNA locus to an interval of approximately 500 kb. These findings suggest that, for the pedigrees from the United States, there are at least two different mutations in the HNA gene.