ABSTRACT
We describe a sensitive ELISA for the measurement, of the recombinant human cytokine, interleukin-3 (IL-3), in human plasma or serum samples. The assay design uses two different anti-IL-3 monoclonal antibodies, giving a two-site assay configuration. The assay incorporates the use of alkaline phosphatase conjugated to streptavidin and a biotinylated anti-IL-3 monoclonal antibody to amplify the resultant signal. This ELISA can measure both glycosylated and non-glycosylated IL-3. The limits of quantification, as determined by precision profiles and quality control samples prepared in 100% human plasma, are 20 pg/ml and 30 pg/ml for non-glycosylated and glycosylated IL-3, respectively.
Subject(s)
Enzyme-Linked Immunosorbent Assay/methods , Interleukin-3/blood , Antibodies, Monoclonal , Antibody Affinity , Glycosylation , Humans , In Vitro Techniques , Interleukin-3/chemistry , Recombinant Proteins/bloodABSTRACT
A rural tertiary care medical center performed 29 reconstructions for Hirschsprung's disease between 1983 and 1992. Long-term follow-up indicates excellent results. The vast majority of patients have maintained continence, are stooling once a day, and require no aids to facilitate stooling. These patients are assessed in relation to sex, mean age, location of transition zone, presence of any associated anomalies, continence, frequency of stooling, and aids required to facilitate stooling.
Subject(s)
Hirschsprung Disease/surgery , Child , Child, Preschool , Colon/surgery , Colon, Sigmoid/surgery , Constipation/etiology , Defecation/physiology , Enema , Female , Follow-Up Studies , Hirschsprung Disease/physiopathology , Humans , Infant , Male , Mineral Oil/therapeutic use , Rectum/surgeryABSTRACT
Twenty-two children with significant blunt abdominal trauma who were admitted to the Surgical Service of an active city-county hospital over the past ten years are presented with discussion of their injuries and treatment. The two mortalities (9%) are presented as case reports. On patient with severe multiple trauma had residual brain damage, and one other patient required a secondary procedure for complications subsequent to the original injury. The results represent an improvement in both complications and mortality when compared to previous reports from similar institutions.
Subject(s)
Abdominal Injuries/therapy , Wounds, Nonpenetrating/therapy , Abdominal Injuries/mortality , Abdominal Injuries/surgery , Adolescent , Child , Child, Preschool , Duodenum/injuries , Humans , Infant , Kidney/injuries , Liver/injuries , Male , Pancreas/injuries , Spleen/injuries , Wounds, Nonpenetrating/mortality , Wounds, Nonpenetrating/surgeryABSTRACT
Blunt abdominal trauma, common in the pediatric patient, often presents diagnostic difficulties. Following proper resuscitation of the patient, the abdomen must be carefully evaluated. Adequate physical examination is often impossible. Computed tomography of the abdomen is preferable in the stable patient, but peritoneal lavage is occasionally useful. Nonoperative therapy is often used in management of solid visceral injuries in children, but this type of therapy necessitates frequent, careful monitoring.
Subject(s)
Abdominal Injuries/diagnosis , Wounds, Nonpenetrating/diagnosis , Abdominal Injuries/complications , Abdominal Injuries/therapy , Child , Fluid Therapy , Hemorrhage/diagnosis , Humans , Peritoneal Cavity , Shock/etiology , Shock/therapy , Therapeutic Irrigation , Tomography, X-Ray Computed , Wounds, Nonpenetrating/complications , Wounds, Nonpenetrating/therapyABSTRACT
Two children are reported with hemangioendothelioma of the hepatobiliary system. One patient, a 7-month-old girl, presented with classic symptomatology of hepatomegaly and congestive heart failure, but did not have any cutaneous lesions. She was treated with systemic steroids and supportive therapy and did very well. The other patient, a 22-month-old boy, is the first patient reported with exclusive involvement of the extrahepatic biliary tree. His symptoms included hepatomegaly and obstructive jaundice. He was evaluated with an endoscopic retrograde cholangiopancreatogram and a transhepatic cholangiogram. He did very well after resection of the lesion and Roux-en-Y drainage of the proximal biliary tree and remains asymptomatic 2 years after surgery.