Glutamate dehydrogenase mediated amino acid metabolism after ammonium uptake enhances rice growth under aeration condition.

KEY MESSAGE: Aeration stimulates the rice growth and nitrogen (N) metabolism; in which, the glutamate accumulation limited by the glutamate dehydrogenase pathway after ammonia uptake may control root N metabolism during aeration. Increasing rhizosphere oxygen content greatly improves rice growth and biomass. To study the intrinsic mechanism involved in nitrogen (N) metabolism, a hydroponic experiment was conducted by supplying two different oxygen levels to two different rice genotypes. Compared to the hypoxia-resistant cultivar (Nip; japonica rice 'Nipponbare'), the hypoxia-sensitive cultivar (U502; upland rice 'Upland 502') presented with severe oxidative damage under the lack of aeration. However, aeration significantly reduced root oxidative damage by enhancing root antioxidant capacity and leaf photosynthesis especially in U502, and significantly increased nitrate (NO3-) and ammonia (NH4+) uptake and upregulated the expression of the genes controlling these processes. Additional NO3- was mainly incorporated into amino acids in the leaves whereas NH4+ assimilation occurred mostly in the roots. The 15N gas chromatography-mass spectrometry analysis demonstrated that aeration had no influence on the compositions of the individual amino acids derived from 15NO3- in the roots, but increased labeled glutamic acid (Glu), asparagine, γ-aminobutyric acid, and alanine in 15NH4+-treated roots. Aeration inhibited root glutamate synthetase activity but this did not inhibit 15N-Glu production from 15NH4+. In contrast, aeration upregulated isocitrate dehydrogenase and glutamate dehydrogenase. These mechanisms and soluble carbohydrates may constitute an alternative pathway for Glu production in which amino acid metabolism is enhanced after NH4+ uptake during aeration. Therefore, the rice growth-enhancing effect of aeration is closely correlated with root redox equilibrium, N uptake, and amino acid metabolism. Glutamic acid accumulation is limited by the glutamate dehydrogenase pathway after NH4+ uptake and may control root N metabolism during aeration.

Variability of leaf photosynthetic characteristics in rice and its relationship with resistance to water stress under different nitrogen nutrition regimes.

The negative effects of water stress on rice can be alleviated by NH4 + nutrition. However, the effects of mixed nitrogen (N) nutrition (NO3 - + NH4 + ) on resistance to water stress are still not well known. To investigate the response of rice growth to water stress and its relationship with photosynthetic characteristics, a hydroponic experiment supplying different N forms was conducted. Compared with NO3 - nutrition, mixed-N and NH4 + nutrition greatly alleviated the reduction of leaf area, chlorophyll content, and photosynthesis under water stress, whilst subsequently maintaining higher biomass. In contrast, water stress inhibited the root-shoot ratios in NH4 + - and mixed-N-supplied plants, indicating reduced root growth and higher photosynthate availability to shoots. The following key observations were made: (1) a similar stomatal limitation and low proportion of activated Rubisco were observed among the three different N nutrition regimes; (2) increased mesophyll conductance in NH4 + - and mixed-N-supplied plants simultaneously stimulated leaf photosynthesis and improved the water use efficiency and (3), the maximum carboxylation rate and actual photochemical efficiency of photosystem II in NH4 + - and mixed-N-supplied plants were significantly higher than that in NO3 - -supplied plants, thus resulting in higher photochemical efficiency under water stress. In conclusion, mixed-N and NH4 + nutrition may be used to develop strategies for improved water stress resistance and stimulated biomass production under conditions of osmotic stress and possibly drought.

Glycine increases cold tolerance in rice via the regulation of N uptake, physiological characteristics, and photosynthesis.

To investigate the response of rice growth and photosynthesis to different nitrogen (N) sources under cold stress, hydroponic cultivation of rice was done in greenhouse, with glycine, ammonium, and nitrate as the sole N sources. The results demonstrate that exposure to low temperature reduced the rice biomass and leaf chlorophyll content, but their values in the glycine-treated plants were significantly higher than in the ammonium- and nitrate-treated plants. This might be attributed to the higher N uptake rate and root area and activity in the glycine-treated plants. The glycine-treated plants also maintained high contents of soluble proteins, soluble sugars, and proline as well as enhanced antioxidant enzyme activities to protect themselves against chilling injury. Under cold stress, reduced stomatal conductance (gs) and effective quantum efficiency of PSII (ΦPSII) significantly inhibited the leaf photosynthesis; however, glycine treatment alleviated these effects compared to the ammonium and nitrate treatments. The high non-photochemical quenching (qN) and excess energy dissipative energy (Ex) in the glycine-treated plants were beneficial for the release of extra energy, thereby, strengthening their photochemical efficiency. We, therefore, conclude that the strengthened cold tolerance of glycine-treated rice plants was closely associated with the higher accumulation of dry matter and photosynthesis through the up-regulation of N-uptake, and increase in the content of osmoprotectants, activities of the antioxidant defense enzymes, and photochemical efficiency. The results of the present study provide new ideas for improving the plant tolerance to extreme temperatures by nutrient resource management in the cold regions.