Impact of miR-519d on the Biological Activity of Non-Small Cell Lung Cancer Cells and Its Mechanism.

Objective: This study aimed to investigate the impact of miR-519d on the biological activity of non-small cell lung cancer (NSCLC) cells and elucidate its underlying mechanism. Methods: An experimental study design was adopted, and a cell culture-based study was conducted. We obtained non-small cell lung cancer cell lines from the ATCC cell bank and categorized them into three groups: the miR group, the NSCLC group, and the Negative control group. Various methods, including flow cytometry, quantitative real-time polymerase chain reaction (qRT-PCR), Transwell assays, Western blotting, and the Cell Counting Kit-8 (CCK-8) assay, were employed to assess miR-519d expression, apoptosis, proliferation, migration, and nuclear factor-kappa B (NF-KB) p65 protein content, thus exploring the impact of miR-519d on the biological activity of NSCLC cells. Results: In the miR group, we observed the highest expression level of miR-519d in NSCLC cells. Furthermore, the miR group exhibited the greatest number of apoptotic cells and the highest apoptosis rate (P < .05). Notably, the Transwell assay revealed reduced migration of NSCLC cells in the miR group, while the NSCLC cells in the control group exhibited more migratory activity. The cell counts of NSCLC cells also significantly decreased in the miR group, with migration comparable to the Negative control group (P > .05). Western blot analysis indicated that NF-KB p65 protein expression was highest in the Negative control group but significantly reduced in the miR group (all P < .05). Conclusions: miR-519d is downregulated in NSCLC cells. Elevating the expression of miR-519d inhibits various biological activities of lung cancer cells, including migration and proliferation. The downregulation of NF-KB p65 likely mediates this inhibition.

Risk Factors and Predictive Efficacy of Visceral Pleural Invasion in Lung Adenocarcinoma Patients with mGGN type.

Objective: To explore the risk factors and predictive effects of visceral pleural invasion in lung adenocarcinoma patients with mGGN type, in order to identify high-risk groups of visceral pleural invasion early, and to provide more references for targeted intervention and individualized treatment adjustment of high-risk groups based on the analysis of risk factors. Methods: The clinical data of 135 patients with mGGN-type lung adenocarcinoma who received surgical treatment in our hospital from January 2018 to December 2022 were selected from our hospital's medical record database for retrospective analysis. The patient information was entered by a two-person summary and analyzed after verification.The patients were divided into invasion group (60 cases) and non-invasion group (75 cases) according to the invasion of pleural viscera, which was helpful to analyze the difference of clinical features between the two groups. The independent risk factors for visceral pleural invasion in patients with mGGN lung adenocarcinoma were evaluated using univariate and multivariate factor methods, and receiver operating characteristic (ROC) curves were drawn to evaluate the clinical efficacy of these risk factors above alone and in combination in predicting of visceral pleural invasion risk. The larger the area under ROC curve, the higher the corresponding sensitivity and specificity, and the greater the predictive value for the risk of visceral pleural invasion in mGGN lung adenocarcinoma patients. Results: Univariate analysis showed that gender, family history of hypertension, location of focus, maximum diameter of solid component, proportion of solid component, pleural indentation, burr sign, bronchial and nodular route may be related to visceral pleural invasion in lung adenocarcinoma patients with mGGN type (P < .05). Multivariate Logistic regression analysis showed that pleural indentation (OR=2.49, 95%CI:1.17~4.58, P < .001) and abnormal broncho-nodular travel (OR=3.06, 95%CI: 1.35~7.02, P = .01) were the independent risk factors for visceral pleural invasion in lung adenocarcinoma patients with mGGN type (P < .05). ROC curve analysis results showed that pleural indentation (AUC=0.70, 95%CI: 0.65~0.79, P = .02), abnormal bronchial and nodular running relationship (AUC=0.74, 95%CI: 0.69~0.81, P = .01) could both be used to predict the visceral pleural invasion risk in lung adenocarcinoma patients with mGGN type, and combined prediction efficacy of both indexes (AUC=0.90, 95%CI: 0.85~0.97, P < .001) was better than single index. Conclusion: The occurrence of visceral pleural infiltration in patients with mGGN lung adenocarcinoma is related to the relationship between pleural indentation and bronchus and nodules. Combined with the above indicators, the risk of visceral pleural infiltration in patients can be effectively predicted, and early intervention and treatment can be performed on high-risk patients accordingly to effectively prevent and treat visceral pleural infiltration.