Notoginsenoside R1 protects boar sperm during liquid storage at 17°C.

Reactive oxygen species (ROS) damage mammalian sperm during liquid storage. Notoginsenoside R1 (NR1) is a compound isolated from the roots of Panax notoginseng; it has powerful ROS-scavenging activities. This work hypothesized that the antioxidant capacity of NR1 could improve boar sperm quality and fertility during liquid storage. During liquid storage at 17°C, the supplementation of semen extender with NR1 (50 µM) significantly improved sperm motility, membrane integrity and acrosome integrity after 5 days of preservation. NR1 treatment also reduced ROS and lipid peroxidation (LPO) levels at day 5 (p <0.05). Higher glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) levels and sperm-zona pellucida binding capacity were observed in the 50 µM NR1 group than those in the control group at day 7 (p <0.05). Importantly, statistical analysis of the fertility of 200 sows indicated that addition of NR1 to the extender improved the fertility parameters of boar spermatozoa during liquid storage at 17°C (p <0.05). These results demonstrate the practical feasibility of using 50 µM NR1 as an antioxidant in boar extender during liquid storage at 17°C, which is beneficial to both spermatozoa quality and fertility.

Rosmarinic acid improves boar sperm quality, antioxidant capacity and energy metabolism at 17°C via AMPK activation.

Boar sperm are susceptible to oxidative damage caused by reactive oxygen species (ROS) during storage. Adenosine monophosphate (AMP)-activated protein kinase (AMPK) is an important therapeutic target, because it is a cellular metabolism energy sensor and key signalling kinase in spermatozoa. We evaluated the effects of rosmarinic acid (RA), an antioxidant, on boar sperm during liquid storage to determine whether it protects boar sperm via AMPK activation. Boar ejaculates were diluted with Modena extender with different concentrations of RA and stored at 17°C for 9 days. Sperm quality parameters, antioxidant capacity, energy metabolism, AMPK phosphorylation and fertility were analysed. Compared with the control, 40 µmol/L significantly improved sperm motility, plasma membrane integrity and acrosome integrity (p < .05). The effective storage time of boar sperm was up to 9 days. On the third and seventh days, the sperm with RA exhibited increased total antioxidant capacity (T-AOC), superoxide dismutase (SOD) activity, adenosine triphosphate (ATP) content, mitochondrial membrane potential (ΔΨm) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity, whereas malondialdehyde (MDA) content was significantly decreased (p < .05). Western blot showed that RA, as well as AICAR (AMPK activator), promoted AMPK phosphorylation, whereas Compound C (AMPK inhibitor) inhibited this effect. The sperm-zona pellucida binding experiment showed that 40 µmol/L RA increased the number of sperm attached to the zona pellucida (p < .05). These findings suggest meaningful methods for improved preservation of boar sperm in vitro and provide new insights into the mechanism by which RA protects sperm cells from oxidative damage via AMPK activation.

Syntax Customized Video Captioning by Imitating Exemplar Sentences.

Enhancing the diversity of sentences to describe video contents is an important problem arising in recent video captioning research. In this paper, we explore this problem from a novel perspective of customizing video captions by imitating exemplar sentence syntaxes. Specifically, given a video and any syntax-valid exemplar sentence, we introduce a new task of Syntax Customized Video Captioning (SCVC) aiming to generate one caption which not only semantically describes the video contents but also syntactically imitates the given exemplar sentence. To tackle the SCVC task, we propose a novel video captioning model, where a hierarchical sentence syntax encoder is first designed to extract the syntactic structure of the exemplar sentence, then a syntax conditioned caption decoder is devised to generate the syntactically structured caption expressing video semantics. As there is no available syntax customized groundtruth video captions, we tackle such a challenge by proposing a new training strategy, which leverages the traditional pairwise video captioning data and our collected exemplar sentences to accomplish the model learning. Extensive experiments, in terms of semantic, syntactic, fluency, and diversity evaluations, clearly demonstrate our model capability to generate syntax-varied and semantics-coherent video captions that well imitate different exemplar sentences with enriched diversities. Code is available at https://github.com/yytzsy/Syntax-Customized-Video-Captioning.

Semantic Conditioned Dynamic Modulation for Temporal Sentence Grounding in Videos.

Temporal sentence grounding in videos aims to localize one target video segment, which semantically corresponds to a given sentence. Unlike previous methods mainly focusing on matching semantics between the sentence and different video segments, in this paper, we propose a novel semantic conditioned dynamic modulation (SCDM) mechanism, which leverages the sentence semantics to modulate the temporal convolution operations for better correlating and composing the sentence-relevant video contents over time. The proposed SCDM also performs dynamically with respect to the diverse video contents so as to establish a precise semantic alignment between sentence and video. By coupling the proposed SCDM with a hierarchical temporal convolutional architecture, video segments with various temporal scales are composed and localized. Besides, more fine-grained clip-level actionness scores are also predicted with the SCDM-coupled temporal convolution on the bottom layer of the overall architecture, which are further used to adjust the temporal boundaries of the localized segments and thereby lead to more accurate grounding results. Experimental results on benchmark datasets demonstrate that the proposed model can improve the temporal grounding accuracy consistently, and further investigation experiments also illustrate the advantages of SCDM on stabilizing the model training and associating relevant video contents for temporal sentence grounding. Our code for this paper is available at https://github.com/yytzsy/SCDM-TPAMI.

α-Ketoglutarate Improves Meiotic Maturation of Porcine Oocytes and Promotes the Development of PA Embryos, Potentially by Reducing Oxidative Stress through the Nrf2 Pathway.

α-Ketoglutarate (α-KG) is a metabolite in the tricarboxylic acid cycle. It has a strong antioxidant function and can effectively prevent oxidative damage. Previous studies have shown that α-KG exists in porcine follicles, and its content gradually increases as the follicles grow and mature. However, the potential mechanism of supplementation of α-KG on porcine oocytes during in vitro maturation (IVM) has not yet been reported. The purpose of this study was to explore the effect of α-KG on the early embryonic development of pigs and the mechanisms underlying these effects. We found that α-KG can enhance the development of early pig embryos. Adding 20 µM α-KG to the in vitro culture medium significantly increased the rate of blastocyst formation and the total cell number. Compared with to that of the control group, apoptosis in blastocysts of the supplement group was significantly reduced. α-KG reduced the production of reactive oxygen species and glutathione levels in cells. α-KG not only improved the activity of mitochondria but also inhibited the occurrence of apoptosis. After supplementation with α-KG, pig embryo pluripotency-related genes (OCT4, NANOG, and SOX2) and antiapoptotic genes (Bcl2) were upregulated. In terms of mechanism, α-KG activates the Nrf2/ARE signaling pathway to regulate the expression of antioxidant-related targets, thus combating oxidative stress during the in vitro culture of oocytes. Activated Nrf2 promotes the transcription of Bcl2 genes and inhibits cell apoptosis. These results indicate that α-KG supplements have a beneficial effect on IVM by regulating oxidative stress during the IVM of porcine oocytes and can be used as a potential antioxidant for IVM of porcine oocytes.