ABSTRACT
Plant proteins are expected to become a major protein source to replace currently used animal-derived proteins in the coming years. However, there are always challenges when using these proteins due to their low water solubility induced by the high molecular weight storage proteins. One approach to address this challenge is to modify proteins through Maillard glycation, which involves the reaction between proteins and carbohydrates. In this review, we discuss various chemical methods currently available for determining the indicators of the Maillard reaction in the early stage, including the graft degree of glycation and the available lysine or sugar, which are involved in the very beginning of the reaction. We also provide a detailed description of the most popular methods for determining graft sites and assessing different plant protein structures and functionalities upon non-enzymatic glycation. This review offers valuable insights for researchers and food scientists in order to develop plant-based protein ingredients with improved functionality.
Subject(s)
Maillard Reaction , Plant Proteins , Animals , FoodABSTRACT
Consumers' preference to have a healthy eating pattern has led to an increasing demand for more nutrient-dense and healthier plant-based foods. Pulse proteins are exceptional quality ingredients with potential nutritional benefits, and might act as health-promoting agents for addressing the new-generation foods. However, the utilization of pulse protein in foods has been hampered by its relatively poor functionality and unpleasant flavor. Protein structure modification has been proved to be a useful means to improve the functionality and flavor profile of pulse protein. This paper begins with a brief introduction of hierarchical structure of pulse protein materials to better understand the structure characteristics. A comprehensive review is presented on the current techniques including chemical and enzymatic modifications and molecular breeding on pulse protein structure and functionality/flavor. The mechanism and the limitations and the toxicological concerns of these approaches are discussed. We conclude that understanding protein structure-functionality relationship is extremely valuable in tailoring proteins for specific functional outcomes and expanding the availability of pulse proteins. Furthermore, selective protein modification is a valuable in-depth toolkit for generating novel protein constructs with preferable functional attributes and flavor profiles. Innovative structure modification with special focus on the molecular basis for the exquisite protein designs is a pillar of pulse protein access to the desired functionality.
Subject(s)
Flavoring Agents , Taste , Consumer Behavior , Food , Food HandlingABSTRACT
This study compared the effects of pan-fried with low (LPF), high (HPF) amounts of oil and deep-fried (DF) on the profiles of advanced glycation end products (AGEs) in sturgeon patties. The surface color of the pan-fried patties, regardless of the amounts of oil used, visually presented more brown than deep-fried ones with higher internal temperature at the frying course of 3-9 min. Compared to LPF and HPF, DF significantly accelerated the furosine development for 6-9 min of frying, dynamically increased the accumulation of CML (Nε-carboxymethyl-lysine) and CEL (Nε-carboxyethyl-lysine) for up to 9 min of frying, and the level of CML in DF than LPF, HPF for 9 min of frying were increased by 209.6 % and 149.9 %, respectively. The oil level employed for pan-fried insignificantly influenced the formation of furosine and CML in patties. The principal component analysis further confirmed that DF patties had a greater influence on the formation of AGEs. The AGEs formation was positively associated with the temperature and amino groups, while remarkably negative correlation with moisture content. Therefore, pan-fried within 6 min of frying was recommended for the domestic cooking of sturgeon patties based on the potential formation of AGEs.
Subject(s)
Fishes , Glycation End Products, Advanced , Animals , Cooking , Principal Component AnalysisABSTRACT
In this paper, a combination of hot-pressure, enzymatic hydrolysis and membrane separation process is used for efficiently and environmentally friendly extraction of chondroitin sulfate (CS) from large hybrid sturgeon cartilage, namely, HPCS. The recovery and yield of CS were 93.68% and 36.47% under the optimized conditions. Fourier transform infrared (FT-IR) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy and high-performance liquid chromatography (HPLC) indicated that the HPCS was composed of monosulfated disaccharides in position 6 and 4 of the N-acetyl-D-galactosamine (58.38% and 27.34%, respectively) and nonsulfated disaccharide (14.29%), which was similar to the composition of CS extracted by dilute alkali-enzymatic hydrolysis-chemical precipitation from large hybrid sturgeon cartilage (SCS). The wound healing results indicated that HPCS could promote cell migration and proliferation, alleviate inflammation and facilitate angiogenesis, which results in its excellent wound treatment activity. These results provide theoretical and practical significance for the production and application of chondroitin sulfate.
Subject(s)
Cartilage , Chondroitin Sulfates , Acceleration , Animals , Cartilage/chemistry , Chondroitin Sulfates/chemistry , Disaccharides/chemistry , Fishes , Spectroscopy, Fourier Transform Infrared , Wound HealingABSTRACT
Yellow pea (Pisum sativum L., YP) grain is generally milled into flour for further processing or direct consumption. However, the comprehensive relationship between milling configurations and YP flour properties remains unclear. The aim of this study is to investigate the effect of configurations (screen aperture size and rotor speed) of ultracentrifugal mill on the physicochemical properties and aromatic profiles of YP flours. Starch damage, morphology, particle size distribution, pasting, thermal property, and aromatic profiles of YP flours were studied. Results show that starch damage increased significantly as the screen aperture size decreased. The YP flour produced with a 500 µm aperture screen had the most stable pasting and thermal properties. With untargeted metabolomic approaches, 2-ethyl-1-hexanol could potentially be applied as an aroma maker to distinguish if an excessive milling or inappropriate configurations of ultracentrifugal mill are applied. This work has furnished fundamentals for the milling and application of YP flour.
Subject(s)
Flour/analysis , Odorants , Pisum sativum/chemistry , Ultracentrifugation/methods , Particle Size , Starch/chemistryABSTRACT
Diverse saccharides are effectively grafted to pea protein isolate (PPI) through Maillard-driven chemistry. The development of conjugates (glyco-PPI) was validated by ultraviolet-visible spectroscopy, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and size exclusion chromatography-high performance liquid chromatography. The impact of covalent conjugation on color development, structural modification, solubility, thermal stability, and volatiles of glycoprotein was examined. The protein solubility was improved, while its thermal stability seemed to be negatively influenced. The principle proposed involves Maillard-driven generation of the conjugates, which enhanced the surface hydrophilicity and unfolding of protein architecture of glyco-PPI. Additionally, both molecular mass and the grafted number of saccharides played a vital role in determining the solubility and thermal stability of glyco-PPI. Protein tends to denature at reaction conditions of 80 °C and pH 10.0, and its cross-linkage occurred in the aqueous system. The two potential routes of molecular interactions between PPI and saccharides were denaturation and glycation or self-cross-linkage. Flavor profile alteration of glycoprotein before and after conjugation was depicted, and relevant off-odors were quantified via headspace solid-phase microextraction gas chromatography-mass spectrometry. These outcomes could furnish valuable in-depth information for dictating functionalities of plant-based protein for food application.
Subject(s)
Pea Proteins/chemistry , Sugars/chemistry , Glycosylation , Hydrogen-Ion Concentration , Hydrophobic and Hydrophilic Interactions , Maillard Reaction , Molecular Weight , SolubilityABSTRACT
Pea protein hydrolysate (PPH) is successfully conjugated with gum arabic (GA) through Maillard-driven chemistry. The effect of cross-linking conjugation on the structure, solubility, volatile substances, emulsification, and antioxidative activity of glyco-PPH is investigated, and found to improve all properties. The formation of glyco-PPH is confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), Fourier-transform infrared (FTIR), and scanning electron microscopy (SEM). Size exclusion chromatography-multi angle light scattering (SEC-MALS) unveils that the maximum molecular mass of glyco-PPH occurs after 1 day of conjugation and approximately 1.2 mol of gum arabic conjugates on one mole of PPH. Headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC-MS) reveals the odor changes of glycoprotein before and after cross-linking. We have also prepared oil-in-water emulsions using glyco-PPH, which have enhanced physical stability against pH changes and chemical stability against lipid oxidation. The mechanism proposed involves Maillard-driven synthesis of the cross-linked PPH-GA conjugates, which increase the surface hydrophilicity and steric hindrance of glyco-PPH. These findings could provide a rational foundation for tailoring the physicochemical properties and functionalities of plant-based protein, which are attractive for food and functional materials applications.
Subject(s)
Flavoring Agents/chemical synthesis , Gum Arabic/chemistry , Pea Proteins/chemistry , Antioxidants/chemistry , Emulsions/chemistry , Flavoring Agents/chemistry , Hydrophobic and Hydrophilic Interactions , Maillard Reaction , Protein Hydrolysates/chemistry , SolubilityABSTRACT
The present work investigated the impact of incubation time (0, 1, 3, and 5â¯day) on the properties and functionalities of conjugates formed between pea protein isolate (PPI) and gum Arabic (GA). The participation of both 11S and 7S to form conjugates with GA was proved by SDS-PAGE. The degree of conjugation reaction of conjugated was characterized by measuring the formation of Maillard reaction products, the loss of free amino groups, and color changes. The results suggested that PPI intimately incorporated into GA after 1â¯day incubation, giving a non-homogeneous microstructure with a reduction of nearly 18% available free amino and an increase of relative solubility to 15.5%. Additionally, emulsions prepared by PPI-GA conjugates showed smaller particle size, higher surface charge, and stronger steric hindrance to stabilize the emulsion droplets against environmental stresses and lipid oxidation. The findings provide a practical means to improve the functionality of pea proteins.
Subject(s)
Acacia/metabolism , Gum Arabic/chemistry , Pea Proteins/chemistry , Emulsions/chemistry , Hydrogen-Ion Concentration , Maillard Reaction , Oils/chemistry , Osmolar Concentration , Oxidation-Reduction , Particle Size , Pea Proteins/metabolism , Solubility , Water/chemistryABSTRACT
Much attention has been given to investigate the formation of Maillard reaction products in thermal processing food due to potential health risks. This study aimed to the profiles of Maillard reaction products (MRPs) and changes of nutrient composition in hairtail (Thichiurus lepturus) fillets prepared by three cooking method: boiling, baking and frying. The Nε-carboxymethyllysine (CML) level ranged from non-detectable to 4.24mg/100g protein and furosine ranged from 4.25 to 20.95mg/100g protein. The levels of CML and furosine in boiled hairtail fillets were much lower than baked and fried ones. The formation of CML was only affected by the cooking method. The changes of the lipid and moisture content, and thiobarbituric acid-reactive substances (TBARS) content in cooked hairtail fillet had different effects on the formation of different stages of Maillard reaction. Furosine level significantly correlated with absorbance in 420nm (r=0.74, p<0.05) and 280nm (r=0.73, p<0.05) and fluorescence Intensity (FI) (r=0.65, p<0.05), but did not correlate with CML. The CML level linearly correlated with the moisture (r=0.79, p<0.01) and lipid content (r=0.73, p<0.05), and the formation of TBARS value (r=0.92, p<0.01), but did not correlate with the FI. Overall, the findings may help to better control the cooking conditions of hairtail meat based on the profiles of MRPs.
Subject(s)
Cooking/methods , Fishes , Food Handling/methods , Glycation End Products, Advanced/analysis , Hot Temperature , Maillard Reaction , Nutritive Value , Seafood/analysis , Animals , Lipids/analysis , Lysine/analogs & derivatives , Lysine/analysis , Thiobarbituric Acid Reactive Substances/analysis , Water/analysisABSTRACT
Recently, much attention has been given to improving the antioxidant activity of protein hydrolysates via the Maillard reaction, but little is known about the cellular antioxidant activity of Maillard reaction products (MRPs) from protein hydrolysates. We first investigated chemical characterization and the cellular antioxidant activity of MRPs in a shrimp (Litopenaeus vannamei) by-product protein hydrolysate (SBH)-glucose system at 110 °C for up to 10 h of heating. Solutions of SBH and glucose were also heated alone as controls. The Maillard reaction greatly resulted in the increase of hydroxymethylfurfural (HMF) and browning intensity, high molecular weight fraction, and reduction of the total amino acid in SBH with the heating time, which correlated well with the free radical scavenging activity of MRPs. MRPs had stronger inhibiting effects on oxidative stress of human HepG2 cells than the original SBH, and its cellular antioxidant activity strongly correlated with free radical scavenging activity, but less affected by the browning intensity and HMF level. The caramelization of glucose partially affected the HMF level and free radical scavenging activity of MRPs, but it was not related to the cellular antioxidant activity. The cellular antioxidant activity of MRPs for 5 h of heating time appeared to reach a maximum level, which was mainly due to carbonyl ammonia condensation reaction. In conclusion, the Maillard reaction is a potential method to increase the cellular antioxidant activity of a shrimp by-product protein hydrolysate, but the higher HMF levels and the lower amino acid content in MRPs should also be considered.