Ficolin-A/2 Aggravates Severe Lung Injury through Neutrophil Extracellular Traps Mediated by Gasdermin D-Induced Pyroptosis.

Neutrophil extracellular traps (NETs) and pyroptosis are critical events in lung injury. This study investigated whether ficolin-A influenced NET formation through pyroptosis to exacerbate lipopolysaccharide (LPS)-induced lung injury. The expression of ficolin-A/2, NETs, and pyroptosis-related molecules was investigated in animal and cell models. Knockout and knockdown (recombinant protein) methods were used to elucidate regulatory mechanisms. The Pearson correlation coefficient was used to analyze the correlation between ficolins and pyroptosis- and NET-related markers in clinical samples. In this study, ficolin-2 (similar to ficolin-A) showed significant overexpression in patients with acute respiratory distress syndrome. In vivo, knockout of Fcna, but not Fcnb, attenuated lung inflammation and inhibited NET formation in the LPS-induced mouse model. DNase I further alleviated lung inflammation and NET formation in Fcna knockout mice. In vitro, neutrophils derived from Fcna-/- mice showed less pyroptosis and necroptosis than those from the control group after LPS stimulation. Additionally, GSDMD knockdown or Nod-like receptor protein 3 inhibitor reduced NET formation. Addition of recombinant ficolin-2 protein to human peripheral blood neutrophils promoted NET formation and pyroptosis after LPS stimulation, whereas Fcn2 knockdown had the opposite effect. Acute respiratory distress syndrome patients showed increased levels of pyroptosis- and NET-related markers, which were correlated positively with ficolin-2 levels. In conclusion, these results suggested that ficolin-A/2 exacerbated NET formation and LPS-induced lung injury via gasdermin D-mediated pyroptosis.

Developing Superior Hydrophobic 3D Hierarchical Electrocatalysts Embedding Abundant Catalytic Species for High Power Density Zn-Air Battery.

It is essential but still challenging to design and construct inexpensive, highly active bifunctional oxygen electrocatalysts for the development of high power density zinc-air batteries (ZABs). Herein, a CoFe-S@3D-S-NCNT electrocatalyst with a 3D hierarchical structure of carbon nanotubes growing on leaf-like carbon microplates is designed and prepared through chemical vapour deposition pyrolysis of CoFe-MOF and subsequent hydrothermal sulfurization. Its 3D hierarchical structure shows excellent hydrophobicity, which facilitates the diffusion of oxygen and thus accelerates the oxygen reduction reaction (ORR) kinetic process. Alloying and sulfurization strategies obviously enrich the catalytic species in the catalyst, including cobalt or cobalt ferroalloy sulfides, their heterojunction, core-shell structure, and S, N-doped carbon, which simultaneously improve the ORR/OER catalytic activity with a small potential gap (ΔE = 0.71 V). Benefiting from these characteristics, the corresponding liquid ZABs show high peak power density (223 mW cm-2 ), superior specific capacity (815 mA h gZn -1 ), and excellent stability at 5 mA cm-2 for ≈900 h. The quasi-solid-state ZABs also exhibit a very high peak power density of 490 mW cm-2 and an excellent voltage round-trip efficiency of more than 64%. This work highlights that simultaneous composition optimization and microstructure design of catalysts can effectively improve the performance of ZABs.

The role of Ficolins in Lung Injury.

BACKGROUND: Respiratory diseases seriously threaten human health worldwide, and lung injury is an important component of respiratory disease. Complement activation is an important function of the innate immune system. Complement activation helps the body defend against invasion by external microorganisms, whereas excessive complement activation can exacerbate tissue damage or lead to unwanted side effects. Ficolins are a class of immune-related proteins in the lectin pathway that play important roles in the body's immune defense. Although individual ficolins are not well understood, current information suggests that ficolins may play an important regulatory role in lung injury. SUMMARY: Several studies have shown that ficolins are involved in the immune response in the lung, particularly in the response to infectious and inflammatory processes. KEY MESSAGES: This review summarizes the role of ficolins in lung injury. Ficolins may influence the development and repair of lung injury by recognizing and binding pathogenic microorganisms, modulating the inflammatory response and promoting the clearance of immune cells. In addition, ficolins are associated with the development and progression of lung diseases (such as pneumonia and ARDS) and may have an important impact on the pathophysiological processes of inflammatory diseases.

Reusable, Stable, Efficient and Multifunctional Superhydrophobic and Oleophilic Polyurethane Sponge for Oil-Water Separation Prepared Using Discarded Composite Insulator.

Oil spills and chemical leakages are a serious source of pollution in oceans and rivers, and have attracted worldwide attention. Many scientists are currently engaged in the development of oil-water separation technology. In this study, the umbrella skirt of a discarded silicone rubber insulator was utilized as feedstock, and polydimethylsiloxane (PDMS) was employed to immobilize the prepared powder (FXBW) onto a polyurethane (PU) sponge skeleton. Without any modifications using chemical reagents, a novel oil-water separation material, FXBW-PU, was developed, with a water contact angle of 155.3°. The FXBW-PU sponge exhibited an absorption capacity ranging from 11.79 to 26.59 g/g for various oils and organic solvents, while maintaining an excellent selective adsorption performance, even after undergoing ten compression cycles, due to its exceptional chemical and mechanical stability. With the assistance of a vacuum pump, the FXBW-PU sponge was utilized in a continuous separation apparatus, resulting in a separation efficiency exceeding 98.6% for various oils and organic solvents. The separation efficiency of n-hexane remains as high as 99.2% even after 10 consecutive separation cycles. Notably, the FXBW-PU sponge also separated the dichloromethane-in-water emulsions, which achieved the effect of purifying water. In summary, FXBW-PU sponge has great potential in the field of cleaning up oil/organic solvent contamination due to its low preparation cost, environmental friendliness and excellent performance.

Ficolin B secreted by alveolar macrophage exosomes exacerbates bleomycin-induced lung injury via ferroptosis through the cGAS-STING signaling pathway.

Pathogenesis exploration and timely intervention of lung injury is quite necessary as it has harmed human health worldwide for years. Ficolin B (Fcn B) is a recognition molecule that can recognize a variety of ligands and play an important role in mediating the cell cycle, immune response, and tissue homeostasis in the lung. However, the role of Fcn B in bleomycin (BLM)-induced lung injury is obscure. This study aims to investigate the sources of Fcn B and its mechanism in BLM-induced lung injury. WT, Fcna-/-, and Fcnb-/- mice were selected to construct the BLM-induced lung injury model. Lung epithelial cells were utilized to construct the BLM-induced cell model. Exosomes that were secreted from alveolar macrophages (AMs) were applied for intervention by transporting Fcn B. Clinical data suggested M-ficolin (homologous of Fcn B) was raised in plasma of interstitial lung disease (ILD) patients. In the mouse model, macrophage-derived Fcn B aggravated BLM-induced lung injury and fibrosis. Fcn B further promoted the development of autophagy and ferroptosis. Remarkably, cell experiment results revealed that Fcn B transported by BLM-induced AMs exosomes accelerated autophagy and ferroptosis in lung epithelial cells through the activation of the cGAS-STING pathway. In contrast, the application of 3-Methyladenine (3-MA) reversed the promotion effect of Fcn B from BLM-induced AMs exosomes on lung epithelial cell damage by inhibiting autophagy-dependent ferroptosis. Meanwhile, in the BLM-induced mice model, the intervention of Fcn B secreted from BLM-induced AMs exosomes facilitated lung injury and fibrosis via ferroptosis. In summary, this study demonstrated that Fcn B transported by exosomes from AMs exacerbated BLM-induced lung injury by promoting lung epithelial cells ferroptosis through the cGAS-STING signaling pathway.

Antidiabetic Effect of Artemether in Db/Db Mice Involves Regulation of AMPK and PI3K/Akt Pathways.

The traditional Chinese medicine has long been used in the treatment of diabetes, one major disease threatening the public health. It has been reported that artemether exerts antidiabetic effects on type 2 diabetes in db/db mice, however the underlying mechanisms remain unknown. In the present study, we show that artemether regulates expression of related enzymes participating in the glucose and lipid metabolism in the liver of db/db mice, which could at least partly explain the improved glucose and lipid metabolism in artemether-treated mice. Additionally, artemether also regulates expression of glycogen synthesis related enzymes in the skeletal muscle of db/db mice, supporting its promotive role in glycogen synthesis. Mechanistically, artemether activates AMPK pathway as well as PI3K/Akt pathway in the liver and skeletal muscle of db/db mice, suggesting that these two signaling pathways are both involved in the antidiabetic effects of artemether on type 2 diabetes in db/db mice. In conclusion, our study connects the antidiabetic effects of artemether to the regulation of metabolic enzymes and signaling pathways, and also provides molecular basis for the potential application of artemether in treating type 2 diabetes.

Efficient Gas Transportation Using Bioinspired Superhydrophobic Yarn as the Gas-Siphon Underwater.

Inspired by the gas-trapped mechanism underwater of Argyroneta aquatica, we prepared a superhydrophobic yarn with a fiber network structure via a facile and environmentally friendly method. Attributed to the low surface energy, the superhydrophobic fiber network structure on the yarn is able to trap and transport bubbles directionally underwater. The functional yarn has good superhydrophobic and superaerophilic properties underwater to realize the directional transport of bubbles underwater without being pumped. We designed demonstration experiments on the antibuoyancy directional bubble transportation, which indicated the feasibility in the applications of gas-related fields. Significantly, on further testing, where the superhydrophobic yarn is put into a U-shaped pipe, we obtain a gas-siphon underwater with a high flux. The superhydrophobic fiber structure yarn can trap the gas underwater to enable the self-starting behavior while no manual intervention is used. The gas-siphon can convey gas over the edge of a vessel and deliver it at a higher level without energy input, which is driven by the differential pressure. The relationship between the differential pressure and the volume flux of transport bubbles is investigated. The experimental results show that the prepared superhydrophobic yarn has the advantages of good stability, easy preparation, and low cost in bubble continuous transportation underwater, which provides a novel strategy for the development and application of new technologies such as directional transportation, separation, exhaustion, and collection of gases in water.

Performance Investigation on Different Designs of Superhydrophobic Surface Texture for Composite Insulator.

To investigate the superhydrophobic properties of different surface textures, nine designs of textures with micro-nanostructures were produced successfully using the laser engraving technique on the surfaces of composite insulator umbrella skirt samples made of silicon rubber. The optimal parameters of the texture designs to give rise to the best hydrophobicity were determined. The surface morphology, abrasion resistance, corrosion resistance, self-cleaning and antifouling property of the different textured surfaces as well as water droplets rolling on the textured surfaces were studied experimentally using a contact angle meter, scanning electron microscope, three-dimensional topography meter and high-speed camera system. It was found that the diamond column design with optimal parameters has the best superhydrophobicity and overall performance. The most remarkable advantage of the optimal diamond column design is its robustness and long-term superhydrophobicity after repeated de-icing in harsh conditions. The reported work is an important step towards achieving superhydrophobic surface without coating for outdoor composite insulator in practical applications.

Robust, heat-resistant and multifunctional superhydrophobic coating of carbon microflowers with molybdenum trioxide nanoparticles.

Superhydrophobic materials have triggered large interest due to their widespread applications, such as self-cleaning, corrosion resistance, anti-icing, and oil/water separation. However, suffering from weak mechanical strength, plenty of superhydrophobic materials are limited in practical application. Herein, we prepared hierarchical carbon microflowers (CMF) dispersed with molybdenum trioxide (MoO3) nanoparticles (MoO3/CMF) via a two-step preparation method. Taking advantage of high-adhesion epoxy resin and the modification with 1H,1H,2H,2H-perfluorodecyltriethoxysilane (PDES), the modified MoO3/CMF (PDES-MoO3/CMF) coating on various substrates shows great waterproof ability, excellent chemical stability, good mechanical durability, and self-cleaning property. More significantly, the prepared PDES-MoO3/CMF powder with high thermal stability (250°C) can be used for oil/water separation due to its special flower-like structure and superhydrophobicity/superoleophilicity. All of these advantages endow the superhydrophobic powders with huge potential in the practical applications.