[Interferon-inducible genes 2', 5'-ollgoadenylate synthetase-like and tetratricopeptide repeats 2 are correlated with clinical features of patients with systemic lupus erythematosus].

OBJECTIVE: To investigate the expression levels of 2', 5'-oligoadenylate synthetase-like (OASL) and interferon induced protein with tetratricopeptide repeats 2 (IFIT2) genes in the peripheral blood leukocyte of patients with systemic lupus erythematosus (SLE), and to evaluate the relations between these gene expression levels and disease activity. METHODS: The clinical data of 50 SLE patients, 25 non-SLE patients with rheumatic diseases, and 25 normal controls were collected. Specimens of peripheral blood were collected; total RNA was extracted and transcribed into cDNA. SYBR green dye based real-time quantitative PCR method was applied to compare the expression levels (indicated as deltaCT value) of OASL and IFIT2 in patients with SLE and those in the controls. RESULTS: The deltaCT value of OASL expression level of the SLE patients was (4.83 +/- 0.41), significantly higher than those of the non-SLE patients (3.26 +/- 0.47) and normal controls (3.07 +/- 0.54, both P < 0.05), The deltaCT value of IFIT2 expression level of the SLE patients was (2.85 +/- 0.41), significantly higher than those of the non-SLE patients (1.19 +/- 0.52) and normal controls (1.07 +/- 0.47, both P < 0.05). The deltaCT value of OASL is related with the SLEDAI scores and immunoglobulin A. (Both P < 0.05). The deltaCT value of IFIT2 is related with the SLEDAI scores and IgA, white blood cell (both P < 0.05). CONCLUSION: The value of IFIT2 and OASL expression level of the SLE patients is up-regulation, the real time expression levels of OASL and IFIT2 genes are associated with SLE disease activity. To inhibit the expression of OASL and IFIT2 may become a novel therapeutic approach for SLE.

[Effect of High Dose Vitamin C on Proliferation and Apoptosis of Acute Myeloid Leukemia Cells].

OBJECTIVE: To investigate the effects of high dose vitamin C on proliferation and apoptosis of acute myeloid leukemia (AML) cell lines including HL-60, U937 and primary CD34+ leukemia cells in AML. METHODS: CD34+ cells were sorted by using immunomagnetic cell sorting system, then the primary CD34+ leukemia cells, including HL-60 and U937 cell lines were cultured in vitro. Cells in each group were treated with different concentrations of vitamin C, the survival rate of cells was determined by MTT assay, the apoptosis rate of cells was evaluated by Annexin V/PI double staining, the expression of apoptotic proteins-including cleaved caspase 3, cleaved caspase-9 and cleaved PARP were detected by Western blot. RESULTS: The proliferation of HL-60 and U937 cells could be inhibited by high dose vitamin C, which showed a concentration-dependent manner (r=-0.9664; r=-0.9796). HL-60 and U937 cells were treated with different concentrations of vitamin C (8 and 20 mmol/L) for 24 hours, respectively, it was found that with the increasing of vitamin C concentration, cell apoptosis rate was significantly increased (r=0.9905; r=0.9971), and the expression of apoptosis related proteins including cleaved caspase 3, cleaved caspase-9 and cleaved PARP was aslo significantly increased with the increasing of concentration. In addition, it was found that with or without the mutation of TET2, high dose vitamin C could inhibit the proliferation (r=-0.9719; r=-0.9699) and promote the apoptosis (r=0.9998; r=0.9901) of primary CD34+ leukemia cells in AML, which showed a dose-dependent manner, but it showed no effect on the proliferation (r=-0.2032) and apoptosis (r=0.1912) of normal CD34+ cells. CONCLUSION: High dose vitamin C can inhibit the proliferation and promote the apoptosis of acute myeloid leukemia cells, and selectively kill primary CD34+ leukemia cells in AML.

[The correlation of myxovirus resistance 1 and 2'5'-ollgoadenylate synthetase 1 with clinical features of systemic lupus erythematosus].

OBJECTIVE: To investigate the expression of myxovirus resistance 1(MX1) and 2'5'-oligoadenylate synthetase 1(OAS1) genes in the peripheral blood leukocyte of patients with systemic lupus erythematosus (SLE), and to evaluated the relations between these genes expression levels and disease activity. METHODS: In this study, there were 50 SLE petients, 20 non-SLE patients with rheumatic dieases, and 25 normal controls. The peripheral blood samples of these patients were collected, and the expression levels (indicated as delta Ct value) of MX1 and OAS1 were measured by Sybr green dye based real-time quantitative PCR method. RESULTS: The deltaCt value of MX1 expression in SLE patients was 3.55 +/- 1.39, which was significantly higher than those of non-SLE patients (2.31 +/- 0.52, P = 0.000) and normal controls (2.23 +/- 1.05, P = 0.000). (2) The deltaCt value of OAS1 expression in SLE patients was 4.45 +/- 1.56, which also was significantly higher than those of non-SLE patients (3.03 +/- 0.76, P = 0.000) and normal controls (2.75 +/- 0.64, P = 0.000). (3) The deltaCt value of OAS1 was correlated with the SLEDAI scores (r = 0.338, P = 0.019) and serum IgA level. (4) The ACt value of MX1 was not correlated with the SLEDAI scores (r = 0.064, P = 0.661), but correlated with the serum levels of triglyceride (TG), cholesterol (TC), low density lipoprotein (LDL), albuminuria of 24 hours (r = 0.428, P = 0.003 r = 0.383, P = 0.009 r = 0.394, P = 0.007; r = 0.316, P = 0.025); (5) The deltaCt values of MX1 and OAS1 in the SLE patients with arthritis were significantly higher than those in non-arthritis SLE patients (3.04 +/- 1.42, P = 0.004; 3.89 +/- 1.49, P = 0.006). CONCLUSION: The expression levels of both MX1 and OAS1 in SLE patients are up-regulated, the expression levels of OAS1 genes are associated with SLE disease activity. As IFN-induced genes, MX1 and OAS1 play their respective role in SLE.

[Determination of aluminum in sediments by atomic absorption spectrophotometer without FIA spectrophotometric analysis].

To search for a new method of determining, we developed a new flow injection analyzer, applied to the atomic absorption spectrophotometer, relying on it without flame in place of visible spectrophotometer, and studied the appropriate condition for the determination of aluminum in sediments, thus built up a kind of new analytical test technique. Three peak and two valley absorption values (A1, A2, A3, A4 and A5) can be continuously obtained simultaneously that all can be used for quantitative analysis, then we discussed its theory and experiment technique. Based on the additivity of absorbance (A = A1+A2+A3+A4+ A5), the sensitivity of FIA is enhanced, and its precision and linear relation are also good, raising the efficiency of AAS. The simple method has been applied to determining Al in sediments, and the results are satisfactory.