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BACKGROUND AND AIMS: The Labrador Teas (genus Rhododendron, subsection Ledum) are a complex of species widely distributed in the Northern Hemisphere. They occupy cold-resistant plant communities from highlands to forest understory and wetland habitats almost circumboreally and they are especially abundant in Northeast Asia (NE Asia) and northern North America (NN Am), still there are no clear species boundaries in this group. The genetic structure of species of the subsect. Ledum from Eurasia and North America as well as the dispersal history of the group require clarification. METHODS: Phylogeny and biogeography of the subsect. Ledum of the genus Rhododendron were assessed using phylogenetic trees constructed based on the analysis of variation in chloroplast petB-petD, trnV-ndhC, trnH-psbA, K2R-K707, atpB oligo2 - rbcL oligo5 and nuclear (ITS1) markers of four Eurasian and one American species (65 populations, 408 individuals). The data were evaluated with Maximum Parsimony and Bayesian analysis. Molecular dating and ancestral areas reconstruction were obtained. KEY RESULTS: Dense sampling revealed widespread presence of shared haplotypes and ribotypes among Ledum populations and species. Two American, three Eurasian and one mixed lineage diversified during the Neogene climate cooling and then rapidly dispersed during the Pleistocene. The ability to accumulate high genetic diversity and to preserve it across distribution ranges and generations prevented Ledum from lineage sorting. As a result, a species complex with a reserve of genetic variability appeared. CONCLUSIONS: Although no clear phylogenetic inference can be obtained at present, the plastid genealogy is consisted with the nuclear genealogy and demonstrates the processes involved in speciation in the Ledum species complex.
ABSTRACT
Recent studies have shown that global variation in body proportions is more complex than previously thought as some traits formerly associated with climate adaptation are better explained by geographic proximity and neutral evolutionary forces. While the recent incorporation of quantitative genetic methodologies has improved understanding of patterns related to climate in Africa, Europe, and the Americas, Asia remains underrepresented in recent and historic studies of body form. As ecogeographic studies tend to focus on male morphology, potential sex differences in features influenced by climate remain largely unexplored. Skeletal measurements encompassing the dimensions of the skull, pelvis, limbs, hands, and feet were collected from male (n = 459) and female (n = 442) remains curated in 13 collections across seven countries in East Asia (n = 901). Osteological data were analyzed with sex and minimum temperature as covariates adjusted by autosomal single-nucleotide polymorphism population genetic distance using univariate Bayesian linear mixed models, and credible intervals were calculated for each trait. Analysis supports a relationship between specific traits and climate as well as providing the magnitude of response in both sexes. After accounting for genetic distance between populations, greater association between climate and morphology was found in postcranial traits, with the relationship between climate and the skull limited primarily to breadth measurements. Larger body size is associated with colder climates with most measurements increasing with decreased temperature. The same traits were not always associated with climate for males and females nor correlated with the same intensity for both sexes. The varied directional association with climate for different regions of the skeleton and between the sexes underscores the necessity of future ecogeographic research to holistically evaluate body form and to look for sex-specific patterns to better understand population responses to environmental stresses.
Subject(s)
Climate , Skull , Female , Male , Humans , Bayes Theorem , Skull/anatomy & histology , Genetic Drift , Cold ClimateABSTRACT
Interannual variation of the aerosol optical depth (AOD) in East Asia has been investigated using Moderate Resolution Imaging Spectroradiometer (MODIS) data and Modern Era Retrospective analysis for Research and Applications Version 2 (MERRA-2) data for 2000-2018. The data analysis focuses on boreal spring when Siberian biomass burning is at its seasonal maximum. The results indicate that the significant increase in organic and black carbon is primarily caused by emissions from biomass burning in East Asia, which leads to significant interannual variations in aerosol loading and pan-Pacific transport. The anomalous large-scale climate variability associated with the East Asia Jet Stream (EAJS) provides favorable conditions for increasing the AOD of organic and black carbon in Northeast Asia and may represent an underlying physical mechanism. When the EAJS shows greater weakening than normal, abnormal high-pressure anomalies are maintained in East Asia, which tend to drive warm advection over Northeast Asia. This warm advection expedites the melting of the Eurasian snow cover, which helps increase surface dryness in late spring and provides favorable conditions for biomass burning. The EAJS index can be predictable with statistical significance up to lead 1 month by the dynamical ensemble seasonal forecasts, suggesting a possible implementation of the empirical AOD forecasts using climate forecast models.
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BACKGROUND: Much research has been done in Northeast Asia to show the efficacy of traditional medicine. While MEDLINE contains many biomedical articles including those on traditional medicine, it does not categorize those articles by specific research area. The aim of this study was to provide a method that searches for articles only on traditional medicine in Northeast Asia, including traditional Chinese medicine, from among the articles in MEDLINE. RESULTS: This research established an SVM-based classifier model to identify articles on traditional medicine. The TAK + HM classifier, trained with the features of title, abstract, keywords, herbal data, and MeSH, has a precision of 0.954 and a recall of 0.902. In particular, the feature of herbal data significantly increased the performance of the classifier. By using the TAK + HM classifier, a total of about 108,000 articles were discriminated as articles on traditional medicine from among all articles in MEDLINE. We also built a web server called DisArticle ( http://informatics.kiom.re.kr/disarticle ), in which users can search for the articles and obtain statistical data. CONCLUSIONS: Because much evidence-based research on traditional medicine has been published in recent years, it has become necessary to search for articles on traditional medicine exclusively in literature databases. DisArticle can help users to search for and analyze the research trends in traditional medicine.
Subject(s)
Biomedical Research/classification , Evidence-Based Medicine , MEDLINE/classification , Medicine, Traditional , Phytotherapy , Publishing , Support Vector Machine , Asia , Biomedical Research/statistics & numerical data , Databases, Factual , Drugs, Chinese Herbal , Herbal Medicine , Humans , Internet , Medicine, Chinese Traditional , Plants, MedicinalABSTRACT
The Oriental fire-bellied toad (Bombina orientalis) is a commonly used study organism, but knowledge of its evolutionary history is incomplete. We analyze sequence data from four genetic markers (mtDNA genes encoding cytochrome c oxidase subunit I, cytochrome b, and 12S-16S rRNA; nuDNA gene encoding recombination activating gene 2) from 188 individuals across its range in Northeast Asia to elucidate phylogeographic patterns and to identify the historic events that shaped its evolutionary history. Although morphologically similar across its range, B. orientalis exhibits phylogeographic structure, which we infer was shaped by geologic, climatic, and anthropogenic events. Phylogenetic and divergence-dating analyses recover four genetically distinct groups of B. orientalis: Lineage 1-Shandong Province and Beijing (China); Lineage 2-Bukhan Mountain (Korea); Lineage 3-Russia, Northeast China, and northern South Korea; and Lineage 4-South Korea. Lineage 2 was previously unknown. Additionally, we discover an area of secondary contact on the Korean Peninsula, and infer a single dispersal event as the origin of the insular Jeju population. Skyline plots estimate different population histories for the four lineages: Lineages 1 and 2 experienced population decreases, Lineage 3 remained stable, while Lineage 4 experienced a sharp increase during the Holocene. The timing of the population expansion of Lineage 4 coincides with the advent of rice cultivation, which may have facilitated the increase in population size by providing additional breeding habitat.
Subject(s)
Agriculture/history , Anura/classification , Anura/genetics , Human Activities/history , Phylogeny , Animals , Cell Nucleus/genetics , China , Cytochromes b/genetics , DNA, Mitochondrial/genetics , Ecosystem , Electron Transport Complex IV/genetics , History, Ancient , Oryza , Phylogeography , Population Dynamics , RNA, Ribosomal/genetics , Republic of Korea , RussiaABSTRACT
Until recently, the settlement of the Americas seemed largely divorced from the out-of-Africa dispersal of anatomically modern humans, which began at least 50,000 years ago. Native Americans were thought to represent a small subset of the Eurasian population that migrated to the Western Hemisphere less than 15,000 years ago. Archeological discoveries since 2000 reveal, however, that Homo sapiens occupied the high-latitude region between Northeast Asia and northwest North America (that is, Beringia) before 30,000 years ago and the Last Glacial Maximum (LGM). The settlement of Beringia now appears to have been part of modern human dispersal in northern Eurasia. A 2007 model, the Beringian Standstill Hypothesis, which is based on analysis of mitochondrial DNA (mtDNA) in living people, derives Native Americans from a population that occupied Beringia during the LGM. The model suggests a parallel between ancestral Native Americans and modern human populations that retreated to refugia in other parts of the world during the arid LGM. It is supported by evidence of comparatively mild climates and rich biota in south-central Beringia at this time (30,000-15,000 years ago). These and other developments suggest that the settlement of the Americas may be integrated with the global dispersal of modern humans.
Subject(s)
Biological Evolution , Human Migration/history , Asia , Climate , History, Ancient , Humans , Models, Theoretical , North AmericaABSTRACT
The fluvio-lacustrine sequences in the Nihewan Basin, northern China provide an important source of Paleolithic settlements and therefore early human occupation in the eastern Old World. Here we present electron spin resonance (ESR) chronology for the Majuangou (MJG-III, MJG-II and MJG-I) and Banshan (BS) Paleolithic sites in this basin by the quartz Ti-Li center ESR dating. Results show that the ages of MJG-III and MJG-II could be estimated to be â¼1.70 Ma (millions of years ago); of MJG-I, â¼1.40 Ma; and BS, â¼1.35 Ma. This ESR chronology is consistent with the previous magnetochronology, thus providing strong support for the earliest human presence at the high northern latitudes of Northeast Asia.
Subject(s)
Archaeology , Chronology as Topic , Hominidae , Animals , China , Electron Spin Resonance SpectroscopyABSTRACT
Genetic diversity and the relationship among nine japonica rice groups consisting of 288 landraces and varieties in different geographical origins of Northeast Asia (China, Japan, Korea, Democratic People's Republic of Korea) and the Russian Far East district of the Russian Federation were evaluated with 154 simple sequence repeat (SSR) markers. A total of 823 alleles were detected. The observed allele numbers (Na) per locus, Nei's gene diversity (He) and the polymorphism information content (PIC) ranged from 2 to 9, 0.061 to 0.869 and 0.060 to 0.856, with an average of 5.344, 0.624 and 0.586, respectively. Five SSR loci, RM1350, RM1369, RM257, RM336 and RM1374, provided the highest PIC values and are potential for exploring the genetic diversity of rice cultivars in Northeast Asia. Molecular variance analysis showed that a significant difference existed both among groups (91.6%) and within each group (8.4%). The low genetic variation within each group indicated that the gene pool is narrow and alien genetic variation should be introduced into the rice breeding program in Northeast Asia. Based on the He and PIC values, the nine groups were ranked in a descending order: Heilongjiang landraces, Jilin landraces, Japanese improved varieties, Heilongjiang improved varieties, Russian Far East district of the Russian Federation improved varieties, Liaoning improved varieties, Jilin improved varieties, Korean improved varieties and Democratic People's Republic of Korea improved varieties. The nine groups were further divided into three subgroups and the 288 varieties into five clusters. This study provided information for parent selection in order to broaden the gene pool of the japonica rice germplasm in Northeast Asia.
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A new species of pholcid spiders, Pholcusfengmeii Zhang, He & Yao, sp. nov. (ââ), is described from Liaoning Province, China. The new species belongs to the speciose phungiformes species group. Taxonomic keys to four closely related species are provided.
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The Mongolian racerunner, Eremias argus, is a small lizard endemic to Northeast Asia that can serve as an excellent model for investigating how geography and past climate change have jointly influenced the evolution of biodiversity in this region. To elucidate the processes underlying its diversification and demography, we reconstructed the range-wide phylogeographic pattern and evolutionary trajectory, using phylogenetic, population genetic, landscape genetic, Bayesian phylogeographic reconstruction and ecological niche modeling approaches. Phylogenetic analyses of the mtDNA cyt b gene revealed eight lineages that were unbounded by geographic region. The genetic structure of E. argus was mainly determined by geographic distance. Divergence dating indicated that E. argus and E. brenchleyi diverged during the Mid-Pliocene Warm Period. E. argus was estimated to have coalesced at~0.4351 Ma (Marine Isotope Stage 19). Bayesian phylogeographic diffusion analysis revealed out-of-Inner Mongolia and rapid colonization events from the end of the Last Interglacial to the Last Glacial Maximum, which is consistent with the expanded suitable range of the Last Glacial Maximum. Pre-Last Glacial Maximum growth of population is presented for most lineages of E. argus. The Glacial Maximum contraction model and the previous multiple glacial refugia hypotheses are rejected. This may be due to an increase in the amount of climatically favorable habitats in Northeast Asia. Furthermore, E. argus barbouri most likely represents an invalid taxon. The present study is the first to report a range-wide phylogeography of reptiles over such a large region in Northeast Asia. Our results make a significant contribution towards understanding the biogeography of the entire Northeast Asia.
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Species of the Pholcusphungiformes group exhibit high diversity in Liaoning Province of northeastern China. This paper summarizes the current knowledge on this species-group from this area. A checklist of 22 species recorded from this province is given, accompanied with a distribution map of the species. Pholcusxiuyan Zhao, Zheng & Yao, sp. nov. (ââ) is described as new to science, and P.yuhuangshan Yao & Li, 2021 is reported from Liaoning for the first time.
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The genus Sinopoda Jäger, 1999 is a group of huntsman spiders (Araneae: Sparassidae: Heteropodinae), and currently seven species have been reported in Korea. In this study, three new species are described from Korea, Sinopodabigibba sp. nov., Sinopodabogil sp. nov., and Sinopodapantherina sp. nov.; Sinopodajirisanensis Kim & Chae, 2013 is revalidated with neotype designation, and had been formerly synonymized with Sinopodaforcipata (Karsch, 1881). Additionally, all previous records of Sinopodastellatops Jäger & Ono, 2002 and S.forcipata from Korea are deemed misidentifications of S.jirisanensis and S.bogil sp. nov., respectively.
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Species biodiversity organises along elevational trends and is generally expected to decrease with increasing elevation. This pattern is regulated by numerous factors, although principally overridden by temperature in ectotherms such as amphibians. Here, we collated elevation data (n = 55,182) collected between 1909 and 2020. We then determined elevation distribution patterns and species communities for all amphibians in the Republic of Korea. Species were found to range from sea level up to 1,393 m a.s.l. The average elevational distribution was significantly different between species but also between anura and caudata. On average, anura were found at lower elevations with a peak in species richness and abundance matching with the lowlands. In opposition, the peak in species richness and abundance for caudata matched with low hilly landscapes. The altitudinal distributions of species were strongly skewed, with all 23 species found within the 0-199 m range, and steadily decreasing with only five species within the last elevational range (1,200-1,399 m). Finally, only a few species were found below 30 m, reflecting a likely risk of salinisation in this environment. Our results help understand the altitudinal distribution of amphibians in the Republic of Korea.
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The ability to migrate is an important biological trait of insects, and wingbeat frequency (WBF) is a key factor influencing migratory behavior. The WBF of insects has been shown to be species-specific in previous studies; however, there is scant information on variations in WBF among different taxa of migratory insects. In 2018 and 2019, we investigated the relationship between WBF and 12 morphological variables (e.g., body mass, body length, total wing area, etc.) of the main migratory insects (77 species in 3 orders and 14 families) over the Bohai Sea in China. The WBF of migratory insects was negatively correlated with the 12 morphological variables and varied significantly among orders. In migratory lepidopterans, neuropterans, and odonatans, the ranges of WBF were 6.71-81.28 Hz, 19.17-30.53 Hz, and 18.35-38.01 Hz, respectively. Regression models between WBF and connecting morphological variables were established for these three orders. Our findings revealed the relationship between WBF and morphometrics of migratory insects in Northeast Asia, increased our knowledge on the flight biology of migratory insects, and provided a basis for developing morphological and WBF-based monitoring techniques to identify migrating insects.
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We propose a method to adjust contributions from upwind emissions to downwind PM2.5 concentrations to account for the differences between observed and simulated PM2.5 concentrations in an upwind area. Emissions inventories (EI) typically have a time lag between the inventory year and the release year. In addition, traditional emission control policies and social issues such as the COVID-19 pandemic cause steady or unexpected changes in anthropogenic emissions. These uncertainties could result in overestimation of the emission impacts of upwind areas on downwind areas if emissions used in modeling for the upwind areas were larger than the reality. In this study, South Korea was defined as the downwind area while other regions in Northeast Asia including China were defined as the upwind areas to evaluate applicability of the proposed adjustment method. We estimated the contribution of emissions released from the upwind areas to PM2.5 concentrations in South Korea from 2015 to 2020 using a three-dimensional photochemical model with two EIs. In these two simulations for 2015-2020, the annual mean foreign contributions differed by 4.1-5.5⯵g/m3. However, after adjustment, the differences decreased to 0.4-1.1⯵g/m3. The adjusted annual mean foreign contributions were 12.7 and 8.8⯵g/m3 during 2015-2017 and 2018-2020, respectively. Finally, we applied the adjustment method to the COVID-19 pandemic period to evaluate the applicability for short-term episodes. The foreign contribution of PM2.5 during the lockdown period in China decreased by 30% after adjustment and the PM2.5 normalized mean bias in South Korea improved from 15% to -4%. This result suggests that the upwind contribution adjustment can be used to alleviate the uncertainty of the emissions inventory used in air quality simulations. We believe that the proposed upwind contribution adjustment method can help to correctly understand the contributions of local and upwind emissions to PM2.5 concentrations in downwind areas.
Subject(s)
Air Pollutants , Air Pollution , COVID-19 , Air Pollutants/analysis , Air Pollution/analysis , Communicable Disease Control , Environmental Monitoring/methods , Humans , Pandemics , Particulate Matter/analysisABSTRACT
Ecological niche modeling is a tool used to determine current potential species' distribution or habitat suitability models which can then be used to project suitable areas in time. Projections of suitability into past climates can identify locations of climate refugia, or areas with high climatic stability likely to contain the highest levels of genetic diversity and stable populations when climatic conditions are less suitable in other parts of the range. Modeling habitat suitability for closely related species in recent past can also reveal potential periods and regions of contact and possible admixture. In the east palearctic, there are five Dryophytes (Hylid treefrog) clades belonging to two groups: Dryophytes japonicus group: Clades A and B; and Dryophytes immaculatus group: Dryophytes immaculatus, Dryophytes flaviventris, and Dryophytes suweonensis. We used maximum entropy modeling to determine the suitable ranges of these five clades during the present and projected to the Last Glacial Maximum (LGM) and Last Interglacial (LIG) periods. We also calculated climatic stability for each clade to identify possible areas of climate refugia. Our models indicated suitable range expansion during the LGM for four clades with the exclusion of D. immaculatus. High climatic stability in our models corresponded to areas with the highest numbers of recorded occurrences in the present. The models produced here can additionally serve as baselines for models of suitability under climate change scenarios and indicate species ecological requirements.
Subject(s)
Ecosystem , Models, Theoretical , Animals , Phylogeography , Population Dynamics , AnuraABSTRACT
The liverwort flora of Ayan was first investigated one hundred and fifty years after the first exploration of vascular plants. A number of factors has determined the relatively high taxonomic diversity of liverworts in this hemiarctic flora of small-sized area: 118 species and one subspecies were revealed. These data are new not only for the studied area, but also for the huge land adjacent to the western coast of the Sea of Okhotsk. The liverwort flora possesses the domination of taxa common in the hemiarctic, although with a lot of taxa more common in boreal as well as arctic-alpine environments. The presence of Mega-Beringian and calciphilous taxa is the peculiar trait of the studied liverwort flora. Based on detrended correspondence analysis (DCA), Ayan liverwort flora shows relationships to the continental mainland floras situated both in North-East Asian hemiarctic and hemiboreal East Asia and is, therefore, the link between both. The flora of Ayan surroundings is one of the newly-filled 'blank spots' in the possible floral exchange way between Arctic Northeast Asia and mountainous floras of temperate East Asia.
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BACKGROUND: The distribution pattern of Pholcusphungiformes species-group indicates that, in Jilin and Heilongjiang, China and North Korea, additional species diversity remains undiscovered. NEW INFORMATION: Pholcusmaxian sp. nov., one new species of P.phungiformes species-group, is described, based on material collected from the borderline between Jilin, China and North Korea. It represents the fifth endemic species of this species-group and the genus Pholcus from this region.
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We conduct a phylogeographic and population genetic study of the Asiatic toad (Bufo gargarizans) to understand its evolutionary history, and the influence of geology and climate. A total of 292 individuals from 94 locations were genotyped for two mitochondrial loci (cytb, ND2) and five nuclear introns (Sox9-2, Rho-3, CCNB2-3, UCH-2, and DBI-2), and we performed a suite of phylogenetic, population genetic, and divergence dating analyses. The phylogenetic trees constructed using mitochondrial loci inferred B. gargarizans being divided into two major groups: China mainland and Northeast Asia (Northeast China, Russia, and Korean Peninsula). As with previous studies of this species, we recover population genetic structure not tied to geographic region. Additionally, we discover a new genetic clade restricted to Northeast Asia that points towards the Korean Peninsula being a glacial refugium during the Pleistocene. The weak phylogeographic pattern of B. gargarizans is likely the result of multiple biological, anthropogenic, and historical factors - robust dispersal abilities as a consequence of physiological adaptations, human translocation, geologic activity, and glacial cycles of the Pleistocene. We highlight the complex geologic and climatic history of Northeast Asia and encourage further research to understand its impact on the biodiversity in the region.
ABSTRACT
Despite 250 years of taxonomic classification and over 1.2 million species already catalogued, known species diversity is only a small part of true species diversity on Earth, and thus, the known species are only the tip of iceberg. Here, we investigated the genus Pholcus Walckenaer, 1805 of the family Pholcidae C. L. Koch, 1850 in the Changbai Mountains, Northeast China, which provides an excellent case of high species diversity. Previously, only 14 endemic Pholcus spiders, all belonging to the P. phungiformes species group, and two introduced species P. manueli Gertsch, 1937 and P. zichyi Kulczynski, 1901 from the P. crypticolens species group, have been recorded from this area. Our study confirmed 11 new species of the P. phungiformes species group based on morphology and three methods of molecular species delimitation: P. gaizhou Yao & Li, sp. nov., P. guanshui Yao & Li, sp. nov., P. jiguanshan Yao & Li, sp. nov., P. longxigu Yao & Li, sp. nov., P. luoquanbei Yao & Li, sp. nov., P. shenshi Yao & Li, sp. nov., P. tianmenshan Yao & Li, sp. nov., P. wangjiang Yao & Li, sp. nov., P. xingqi Yao & Li, sp. nov., P. yaoshan Yao & Li, sp. nov., and P. yuhuangshan Yao & Li, sp. nov. This study brings the fauna of the P. phungiformes species group from the Changbai Mountains to 25 species, approximately two times more than previously known, which could indicate that species diversity in the area is underestimated for all arthropod fauna.