Phenotypic limits of crop diversity: a data exploration of functional trait space.

Relationships between crop genetic and functional diversity are key to addressing contemporary agricultural challenges. Yet, there are few approaches for quantifying the relationship between genetic diversity and crop functional trait expression. Here, we introduce 'functional space accumulation curves' to analyze how trait space increases with the number of crop genotypes within a species. We explore the potential for functional space accumulating curves to quantify genotype-trait space relationships in four common annual crop species: barley (Hordeum vulgare), rice (Oryza sativa), soybean (Glycine max), and durum wheat (Triticum durum). We also employ these curves to describe genotype-trait space relationships in the wild annual Arabidopsis thaliana, which has not been subjected to artificial selection. All five species exhibited asymptotic functional space accumulation curves, suggesting a limit to intraspecific functional crop diversity, likely due to: dominant phenotypes represented by several genotypes; or functional redundancy that might exist among genotypes. Our findings indicate that there is a diminishing return of functional diversity with increasing number of genotypes. Our analysis demonstrates the efficacy of functional space accumulation curves in quantifying trait space occupancy of crops, with implications for managing crop diversity in agroecosystems, and genetic diversity in crop breeding programs.

A genomic journey across the past, present, and future of South American maize.

Maize (Zea mays ssp. mays) diverged from one of its wild relatives, the teosinte Zea mays ssp. parviglumis, in the lowlands of southwest Mexico approximately 9000 years ago. Following this divergence, maize rapidly expanded throughout the Americas, becoming a staple food. This dispersal was accompanied by significant demographic and selective changes, leading to the development of numerous local varieties with a complex evolutionary history that remains incompletely understood. In recent years, genomic advances have challenged traditional models of maize domestication and spread to South America. At least three distinct genetic lineages associated with different migratory waves have been described: ancestral Andean, ancestral Lowland, and Pan-American. Additionally, the significant role of the teosinte Zea mays ssp. mexicana, in the evolution of modern maize has been recently uncovered. Genomic studies have shed light into highland adaptation processes, revealing largely independent adaptation events in Meso- and South America. As new evidence emerges, the regional complexity underlying maize diversity and the need for comprehensive, multi-scale approaches become evident. In the face of climate change and evolving agricultural landscapes, the conservation of native maize in South America is of growing interest, with genomics serving as an invaluable tool for identifying and preserving the genetic variability of locally adapted germplasm.

Maintenance and expansion of genetic and trait variation following domestication in a clonal crop.

Clonal propagation enables favourable crop genotypes to be rapidly selected and multiplied. However, the absence of sexual propagation can lead to low genetic diversity and accumulation of deleterious mutations, which may eventually render crops less resilient to pathogens or environmental change. To better understand this trade-off, we characterize the domestication and contemporary genetic diversity of Enset (Ensete ventricosum), an indigenous African relative of bananas (Musa) and a principal starch staple for 20 million Ethiopians. Wild enset reproduction occurs strictly by sexual outcrossing, but for cultivation, it is propagated clonally and associated with diversification and specialization into hundreds of named landraces. We applied tGBS sequencing to generate genome-wide genotypes for 192 accessions from across enset's cultivated distribution, and surveyed 1340 farmers on enset agronomic traits. Overall, reduced heterozygosity in the domesticated lineage was consistent with a domestication bottleneck that retained 37% of wild diversity. However, an excess of putatively deleterious missense mutations at low frequency present as heterozygotes suggested an accumulation of mutational load in clonal domesticated lineages. Our evidence indicates that the major domesticated lineages initially arose through historic sexual recombination associated with a domestication bottleneck, followed by the amplification of favourable genotypes through an extended period of clonal propagation. Among domesticated lineages, we found a significant phylogenetic signal for multiple farmer-identified food, nutrition and disease resistance traits and little evidence of contemporary recombination. The development of future-climate adapted genotypes may require crop breeding, but outcrossing risks exposing deleterious alleles as homozygotes. This trade-off may partly explain the ubiquity and persistence of clonal propagation over recent centuries of comparative climate stability.

The genomic and bioclimatic characterization of Ethiopian barley (Hordeum vulgare L.) unveils challenges and opportunities to adapt to a changing climate.

The climate crisis is impacting agroecosystems and threatening food security of millions of smallholder farmers. Understanding the potential for current and future climatic adaptation of local crop agrobiodiversity may guide breeding efforts and support resilience of agriculture. Here, we combine a genomic and climatic characterization of a large collection of traditional barley varieties from Ethiopia, a staple for local smallholder farmers cropping in challenging environments. We find that the genomic diversity of barley landraces can be partially traced back to geographic and environmental diversity of the landscape. We employ a machine learning approach to model Ethiopian barley adaptation to current climate and to identify areas where its existing diversity may not be well adapted in future climate scenarios. We use this information to identify optimal trajectories of assisted migration compensating to detrimental effects of climate change, finding that Ethiopian barley diversity bears opportunities for adaptation to the climate crisis. We then characterize phenology traits in the collection in two common garden experiments in Ethiopia, using genome-wide association approaches to identify genomic loci associated with timing of flowering and maturity of the spike. We combine this information with genotype-environment associations finding that loci involved in flowering time may also explain environmental adaptation. Our data show that integrated genomic, climatic, and phenotypic characterizations of agrobiodiversity may provide breeding with actionable information to improve local adaptation in smallholder farming systems.

Diversity buffers winegrowing regions from climate change losses.

Agrobiodiversity-the variation within agricultural plants, animals, and practices-is often suggested as a way to mitigate the negative impacts of climate change on crops [S. A. Wood et al., Trends Ecol. Evol. 30, 531-539 (2015)]. Recently, increasing research and attention has focused on exploiting the intraspecific genetic variation within a crop [Hajjar et al., Agric. Ecosyst. Environ. 123, 261-270 (2008)], despite few relevant tests of how this diversity modifies agricultural forecasts. Here, we quantify how intraspecific diversity, via cultivars, changes global projections of growing areas. We focus on a crop that spans diverse climates, has the necessary records, and is clearly impacted by climate change: winegrapes (predominantly Vitis vinifera subspecies vinifera). We draw on long-term French records to extrapolate globally for 11 cultivars (varieties) with high diversity in a key trait for climate change adaptation-phenology. We compared scenarios where growers shift to more climatically suitable cultivars as the climate warms or do not change cultivars. We find that cultivar diversity more than halved projected losses of current winegrowing areas under a 2 °C warming scenario, decreasing areas lost from 56 to 24%. These benefits are more muted at higher warming scenarios, reducing areas lost by a third at 4 °C (85% versus 58%). Our results support the potential of in situ shifting of cultivars to adapt agriculture to climate change-including in major winegrowing regions-as long as efforts to avoid higher warming scenarios are successful.

The scale dependency of spatial crop species diversity and its relation to temporal diversity.

Increasing crop species diversity can enhance agricultural sustainability, but the scale dependency of the processes that shape diversity and of the effects of diversity on agroecosystems is insufficiently understood. We used 30 m spatial resolution crop classification data for the conterminous United States to analyze spatial and temporal crop species diversity and their relationship. We found that the US average temporal (crop rotation) diversity is 2.1 effective number of species and that a crop's average temporal diversity is lowest for common crops. Spatial diversity monotonically increases with the size of the unit of observation, and it is most strongly associated with temporal diversity when measured for areas of 100 to 400 ha, which is the typical US farm size. The association between diversity in space and time weakens as data are aggregated over larger areas because of the increasing diversity among farms, but at intermediate aggregation levels (counties) it is possible to estimate temporal diversity and farm-scale spatial diversity from aggregated spatial crop diversity data if the effect of beta diversity is considered. For larger areas, the diversity among farms is usually much greater than the diversity within them, and this needs to be considered when analyzing large-area crop diversity data. US agriculture is dominated by a few major annual crops (maize, soybean, wheat) that are mostly grown on fields with a very low temporal diversity. To increase crop species diversity, currently minor crops would have to increase in area at the expense of these major crops.

Examining linkages among multiple sustainable development outcomes: does the productive safety net program increase on-farm agrobiodiversity?

In Ethiopia, on-farm agrobiodiversity and the Productive Safety Net Program (PSNP) play a key role in building smallholders' resilience. However, the impact of PSNP on on-farm agrobiodiversity is not yet well investigated. In this paper, we develop an analytical framework that links PSNP participation to on-farm agrobiodiversity. Both diverse farming systems and PSNP require labour inputs while providing income stabilization, which might result in a negative relationship between the two. Conversely, higher income from PSNP might allow farmers to increase their long-term on-farm investments, as opposed to the strategies oriented toward the highest immediate profit or calorie intake outcome. We base our empirical analysis on the World Bank's Ethiopian Socioeconomic Survey, a panel dataset encompassing nearly 3000 respondents and a Tobit model, based on Difference-in-Difference and the Propensity-Score Matching methods. We find that Ethiopia's PSNP has a negative impact on farm labour input, both in terms of labour intensity and duration. Furthermore, our results show that participation in the program is associated, on average, with lower on-farm crop diversity. We conclude that the PSNP participation may be crowding-out production stabilizing farming activities, such as intercropping or cover cropping, that are more labour intensive. Our findings call for embedding tools in the new phase of the PSNP (2021-2025) that could incentivise on-farm resilience-oriented investments, in particular leading to higher crop diversification.

Do carbon stocks and floristic diversity of tropical homegardens vary along an elevational gradient and based on holding size in central Kerala, India?

Homegarden (HG) agroforestry combines biological carbon (C) sequestration with biodiversity conservation outcomes. Although C stocks and species richness of HGs vary along elevational gradients and as a function of holding sizes, there is no consensus on the nature and magnitude of such variations. Field studies were conducted in the Western Ghats region of central Kerala, India (180 homesteads in 20 selected panchayats), to evaluate the effects of elevation (near sea level to 1938 m) and garden size (162-10,117 m2) on aboveground C stocks and floristic diversity. The C stocks (per unit area) of HGs (arborescent species) were highly variable (0.63-93.65 Mg ha-1), as garden management was highly individualistic and it exhibited a weak negative relationship with elevation. Likewise, there was a weak negative relationship between C stocks and garden size. Tree stocking levels (stems/garden) and species richness (species/garden) positively impacted total C stocks per garden. Floristic diversity was high in the study area (753 species) and included many rare and endangered species (43 IUCN Red-Listed species) making homegardens circa situm reservoirs of biodiversity. Elevation and holding size exerted a weak negative linear relationship on Simpson's floristic diversity index, which ranged from 0.26 to 0.93 for the arboreal species. Homegardens, regardless of elevation or size, contribute to C sequestration and agrobiodiversity conservation and help achieve the UN Sustainable Development Goals (SDGs), particularly Climate Action (SDG-13) and conserving agrobiodiversity (SDG-15, Life on Land).

Crop genetic erosion: understanding and responding to loss of crop diversity.

Crop diversity underpins the productivity, resilience and adaptive capacity of agriculture. Loss of this diversity, termed crop genetic erosion, is therefore concerning. While alarms regarding evident declines in crop diversity have been raised for over a century, the magnitude, trajectory, drivers and significance of these losses remain insufficiently understood. We outline the various definitions, measurements, scales and sources of information on crop genetic erosion. We then provide a synthesis of evidence regarding changes in the diversity of traditional crop landraces on farms, modern crop cultivars in agriculture, crop wild relatives in their natural habitats and crop genetic resources held in conservation repositories. This evidence indicates that marked losses, but also maintenance and increases in diversity, have occurred in all these contexts, the extent depending on species, taxonomic and geographic scale, and region, as well as analytical approach. We discuss steps needed to further advance knowledge around the agricultural and societal significance, as well as conservation implications, of crop genetic erosion. Finally, we propose actions to mitigate, stem and reverse further losses of crop diversity.

Genetic diversity, gene flow, and differentiation among wild, semiwild, and landrace chile pepper (Capsicum annuum) populations in Oaxaca, Mexico.

PREMISE: Capsicum annuum (Solanaceae) was originally domesticated in Mexico, where wild (C. annuum var. glabriusculum) and cultivated (C. annuum var. annuum) chile pepper populations (>60 landraces) are common, and wild-resembling individuals (hereafter semiwild) grow spontaneously in anthropogenic environments. Here we analyze the role of elevation and domestication gradients in shaping the genetic diversity in C. annuum from the state of Oaxaca, Mexico. METHODS: We collected samples of 341 individuals from 28 populations, corresponding to wild, semiwild (C. annuum var. glabriusculum) and cultivated C. annuum, and closely related species Capsicum frutescens and C. chinense. From the genetic variation of 10 simple sequence repeat (SSR) loci, we assessed the population genetic structure, inbreeding, and gene flow through variance distribution analyses, genetic clustering, and connectivity estimations. RESULTS: Genetic diversity (HE ) did not differ across domestication levels. However, inbreeding coefficients were higher in semiwild and cultivated chiles than in wild populations. We found evidence for gene flow between wild populations and cultivated landraces along the coast. Genetic structure analysis revealed strong differentiation between most highland and lowland landraces. CONCLUSIONS: Gene flow between wild and domesticated populations may be mediated by backyards and smallholder farms, while mating systems may facilitate gene flow between landraces and semiwild populations. Domestication and elevation may overlap in their influence on genetic differentiation. Lowland Gui'ña dani clustered with highland landraces perhaps due to the social history of the Zapotec peoples. In situ conservation may play an important role in preserving semiwild populations and private alleles found in landraces.

Dietary diversity and its determinants among Khasi and Garo indigenous women (15 to 49 years) in Meghalaya, northeast India.

BACKGROUND: Despite generally high agrobiodiversity, the Khasi and Garo indigenous people in Meghalaya have poor nutritional status among children and women. AIM: To assess the dietary diversity of the Khasi and Garo indigenous women of reproductive age in Meghalaya, while examining the associated factors that affect it and to map the diversity of local food plants. METHODS: A dietary survey was conducted through 24-hour recall with 276 women from 28 villages of Meghalaya. The mapping of local food plants was conducted through key informant interviews and focus group discussions in the same villages. RESULTS: The mean minimum dietary diversity for women is 4.22 (SD ±1.26); with one-third (37%) of the women attaining a minimum dietary diversity of five or more food groups in a day. There were significant differences in the dietary diversity among the indigenous groups, the Garo community attaining higher minimum dietary diversity for women. There was also an inverse association between the number of land uses and dietary diversity. The mean number of food plants (including crop varieties) found in each village is 203 (SD±45.7). CONCLUSIONS: There is potential to enhance dietary diversity through diversifying lesser consumed crops such as vitamin A-rich vegetables and fruits, nuts, seeds and green leafy vegetables in existing land holdings. Also, proper management and access to the commons will provide a sustainable resource base for diet and food security, enhance dietary diversity and bridge the hidden hunger among children and women.

Agro-ecology science relates to economic development but not global pesticide pollution.

Synthetic pesticides are core features of input-intensive agriculture and act as major pollutants driving environmental change. Agroecological science has unveiled the benefits of biodiversity for pest control, but research implementation at the farm-level is still difficult. Here we address this implementation gap by using a bibliometric approach, quantifying how countries' scientific progress in agro-ecology relates to pesticide application regimes. Among 153 countries, economic development does spur scientific innovation but irregularly bears reductions in pesticide use. Some emerging economies bend the Environmental Kuznets curve (EKC) - the observed environmental pollution by a country's wealth - for pesticides and few high-income countries exhibit a weak agro-ecology 'technique effect'. Our findings support recent calls for large-scale investments in nature-positive agriculture, underlining how agro-ecology can mend the ecological resilience, carbon footprint, and human health impacts of intensive agriculture. Yet, in order to effectively translate science into practice, scientific progress needs to be paralleled by policy-change, farmer education and broader awareness-raising.

Homegarden agroforestry systems in achievement of Sustainable Development Goals. A review.

Homegarden, a type of agroforestry system, is one of the earliest thriving traditional food systems reported. Studying the contribution of homegardens in the context of Sustainable Development Goals (SDGs) is crucial when the COVID-19 pandemic has hindered the achievement of many of the crucial SDGs. In this review, we focused on 94 peer-reviewed papers on homegardens from 2010 to 2021 to interrelate them with the corresponding targets and indicators of each SDG. The SDGs were classified into five categories, each focusing on a specific aspect: Category 1 (SDGs 1-5, poverty dimension), Category 2 (SDGs 6-9, development infrastructures), Category 3 (SDGs 10-12, sustainable production and consumption), Category 4 (SDGs 13-15, green infrastructures), and Category 5 (SDGs 16-17, green institutions). The distribution of the 94 papers analyzed was 92%, 23%, 33%, 51%, and 50% in each of the SDG categories, respectively. Category 1 and SDG 2 were found to be most realized in the homegarden literature. Important observations were found that highlight homegardens' probable use in providing food security, nutritional needs, health and wellness, preservation of agrobiodiversity, and enduring sustainability. Homegardens appear to be an important strategy for attaining the SDGs and can be accomplished with proper planning, in addition to taking into consideration how the traditional societies have sustained it for long. Supplementary Information: The online version contains supplementary material available at 10.1007/s13593-022-00781-9.

Climate change in the Catalan Pyrenees intersects with socioeconomic factors to shape crop diversity and management.

Most studies on climate change's impacts on agriculture focus on modeling techniques based on large-scale meteorological data, while few have investigated how farmer's perception of climate change's impacts can affect crop diversity and crop management practices, especially in industrialized contexts. To fill this gap, we conducted 24 semi-structured interviews in a study site located in the Catalan Pyrenees. Our results show for the first time in an industrialized context that farmers perceive multiple interrelated climate change impacts on local agroecosystems. For instance, snowfall and freeze events have decreased, which respondents associated with the increase of pests and diseases affecting both wild flora and cultivated plants. Similarly, changes in precipitation patterns lead to a perceived decrease in useful rain for agriculture. Farmers are also reporting changes in their management practices, such as increased irrigation or use of pesticides, which respond to these climatic factors but also to changes in the crops that are cultivated. Crop diversity is in decline in the area both at the species and landrace levels, especially in rainfed fields. This is mainly driven by socioeconomic factors such as agricultural abandonment or access to commercial seeds, although climate change factors such as increased pests or decreased rainfall can have an impact. Despite the crop diversity losses found, many landraces have been maintained, mainly due to their cultural value, and also new crop species have been introduced, which are now viable due to the increase in temperature. Although we focused on a specific case study, we found several trends that are also present in other contexts. Therefore, the results of this research are relevant at a global scale since they show that climate change is affecting mountain agroecosystems in industrialized contexts and may affect more drastically both agrobiodiversity and crop management practices in agroecosystems worldwide. Supplementary Information: The online version contains supplementary material available at 10.1007/s13593-022-00806-3.

Genome wide association study of agronomic and seed traits in a world collection of proso millet (Panicum miliaceum L.).

BACKGROUND: The climate crisis threatens sustainability of crop production worldwide. Crop diversification may enhance food security while reducing the negative impacts of climate change. Proso millet (Panicum milaceum L.) is a minor cereal crop which holds potential for diversification and adaptation to different environmental conditions. In this study, we assembled a world collection of proso millet consisting of 88 varieties and landraces to investigate its genomic and phenotypic diversity for seed traits, and to identify marker-trait associations (MTA). RESULTS: Sequencing of restriction-site associated DNA fragments yielded 494 million reads and 2,412 high quality single nucleotide polymorphisms (SNPs). SNPs were used to study the diversity in the collection and perform a genome wide association study (GWAS). A genotypic diversity analysis separated accessions originating in Western Europe, Eastern Asia and Americas from accessions sampled in Southern Asia, Western Asia, and Africa. A Bayesian structure analysis reported four cryptic genetic groups, showing that landraces accessions had a significant level of admixture and that most of the improved proso millet materials clustered separately from landraces. The collection was highly diverse for seed traits, with color varying from white to dark brown and width spanning from 1.8 to 2.6 mm. A GWAS study for seed morphology traits identified 10 MTAs. In addition, we identified three MTAs for agronomic traits that were previously measured on the collection. CONCLUSION: Using genomics and automated seed phenotyping, we elucidated phylogenetic relationships and seed diversity in a global millet collection. Overall, we identified 13 MTAs for key agronomic and seed traits indicating the presence of alleles with potential for application in proso breeding programs.

Positive but variable effects of crop diversification on biodiversity and ecosystem services.

Ecological theory suggests that biodiversity has a positive and stabilizing effect on the delivery of ecosystem services. Yet, the impacts of increasing the diversity of cultivated crop species or varieties in agroecosystems are still under scrutiny. The available empirical evidence is scattered in scope, agronomic and geographic contexts, and impacts on ecosystem services may depend on the type of diversification strategy used. To robustly assess the effects of crop diversification in agroecosystems, we compiled the results of 95 meta-analyses integrating 5156 experiments conducted over 84 experimental years and representing more than 54,500 paired observations on 120 crop species in 85 countries. Overall, our synthesis of experimental data from across the globe shows that crop diversification enhances not only crop production (median effect +14%) but also the associated biodiversity (+24%, i.e., the biodiversity of non-cultivated plants and animals), and several supporting and regulating ecosystem services including water quality (+51%), pest and disease control (+63%) and soil quality (+11%). However, there was substantial variability in the results for each individual ecosystem service between different diversification strategies such as agroforestry, intercropping, cover crops, crop rotation or variety mixtures. Agroforestry is particularly effective in delivering multiple ecosystem services, that is, water regulation and quality, pest and diseases regulation, associated biodiversity, long-term soil productivity and quality. Variety mixtures, instead, provide the lowest benefits, whereas the other strategies show intermediate results. Our results highlight that while increasing the diversity of cultivated crop species or varieties in agroecosystems represents a very promising strategy for more sustainable land management, contributing to enhanced yields, enhanced biodiversity and ecosystem services, some crop diversification strategies are more effective than others in supporting key ecosystem services.

Taxonomic and functional homogenization of farmland birds along an urbanization gradient in a tropical megacity.

Urbanization is a major driver of land use change and biodiversity decline. While most of the ongoing and future urbanization hotspots are located in the Global South, the impact of urban expansion on agricultural biodiversity and associated functions and services in these regions has widely been neglected. Additionally, most studies assess biodiversity responses at local scale (α-diversity), however, ecosystem functioning is strongly determined by compositional and functional turnover of communities (ß-diversity) at regional scales. We investigated taxonomic and functional ß-diversity of farmland birds across three seasons on 36 vegetable farms spread along a continuous urbanization gradient in Bangalore, a South Indian megacity. Increasing amount of grey area in the farm surroundings was the dominant driver affecting ß-diversity and resulting in taxonomic and functional homogenization of farmland bird communities. Functional diversity losses were higher than expected from species declines (i.e., urbanization acts as an environmental filter), with particular losses of functionally important groups such as insectivores of crop pests. Moreover, urbanization reduced functional redundancy of bird communities, which may further weaken ecosystems resilience to future perturbations. Our study underscores urbanization as a major driver of taxonomic and functional homogenization of species communities in agricultural systems, potentially threatening crucial ecosystem services for food production.

A Nutrition-Sensitive Agroecology Intervention in Rural Tanzania Increases Children's Dietary Diversity and Household Food Security But Does Not Change Child Anthropometry: Results from a Cluster-Randomized Trial.

BACKGROUND: There are urgent calls for the transformation of agriculture and food systems to address human and planetary health issues. Nutrition-sensitive agriculture and agroecology promise interconnected solutions to these challenges, but evidence of their impact has been limited. OBJECTIVES: In a cluster-randomized trial (NCT02761876), we examined whether a nutrition-sensitive agroecology intervention in rural Tanzania could improve children's dietary diversity. Secondary outcomes were food insecurity and child anthropometry. We also posited that such an intervention would improve sustainable agricultural practices (e.g., agrobiodiversity, intercropping), women's empowerment (e.g., participation in decision making, time use), and women's well-being (e.g., dietary diversity, depression). METHODS: Food-insecure smallholder farmers with children aged <1 y from 20 villages in Singida, Tanzania, were invited to participate. Villages were paired and publicly randomized; control villages received the intervention after 2 y. One man and 1 woman "mentor farmer" were elected from each intervention village to lead their peers in agroecological learning on topics including legume intensification, nutrition, and women's empowerment. Impact was estimated using longitudinal difference-in-differences fixed-effects regression analyses. RESULTS: A total of 591 households (intervention: n = 296; control: n = 295) were enrolled; 90.0% were retained to study end. After 2 growing seasons, the intervention improved children's dietary diversity score by 0.57 food groups (out of 7; P < 0.01), and the percentage of children achieving minimum dietary diversity (≥4 food groups) increased by 9.9 percentage points during the postharvest season. The intervention significantly reduced household food insecurity but had no significant impact on child anthropometry. The intervention also improved a range of sustainable agriculture, women's empowerment, and women's well-being outcomes. CONCLUSIONS: The magnitude of the intervention's impacts was similar to or larger than that of other nutrition-sensitive interventions that provided more substantial inputs but were not agroecologically focused. These data suggest the untapped potential for nutrition-sensitive agroecological approaches to achieve human health while promoting sustainable agricultural practices.

Wildlife-friendly food requires a multi-stakeholder approach to deliver landscape-scale biodiversity conservation in the Satoyama landscape of Japan.

Many global biodiversity hotspots have been cultivated for food for centuries and their unique agrobiodiversity is now under threat from land-use conversion, land abandonment or agricultural intensification. Wildlife-friendly farming (WFF) certification is a market-based approach that aims to alleviate the threats through charging a premium over conventional food products. This study explores the economic demand for WFF to protect biodiversity and maintain traditional rice cultivation in the Satoyama landscape of Japan by quantifying the price differential for key attributes of a landscape scale WFF scheme using choice experiments with consumers. A novel component of this study was to combine the choice experiment data with qualitative interviews with stakeholders together with observational and participatory approaches to identify underlying motivations for purchase decisions and to assess using a mixed methods approach the potential of WFF schemes to support landscape scale conservation and rural development. We found that consumer's willingness to pay (WTP) for organic rice was the highest, with a premium of 2937 JPY (26.83 USD) compared to non-organic rice. Respondents were also willing to pay more for all rice that conserves individual target species, with WTP for bird species the highest and for rice produced specifically in the traditional Satoyama landscapes. Although a WFF-Satoyama programme would bring public benefits and support rural livelihoods we suggest there are several challenges to widespread adoption that include an ageing farming population, a lack of appropriate business skills and technical capacity, and obstacles arising from Japanese land use policies concerning forestry and hunting.

Steering the restoration of degraded agroecosystems during the United Nations Decade on Ecosystem Restoration.

Agroecosystems are the largest human-natural coupled production system covering ~40% of the planet earth and provide essential ecosystem services for a good quality of life and human wellbeing. The sustainable management of agroecosystems are therefore essential for meeting the food, fuel, fiber, and fodder demands of the rapidly growing human population. Agroecosystems also play a key role in trace gases emission, and also affect the quality and usage of life-supporting resources such as air, water, soil etc. Though the sustainable management of agroecosystems are imperative for achieving UN-Sustainable Development Goals, they are frequently under degradation due to multiple drivers of changes such as unsustainable land-use practices, biodiversity loss, pollution and climate change, etc. Therefore, cordial efforts at national, regional and global levels are essential for managing agroecosystems to meet out the global goals and also the targets of the United Nations- Decade o-n Ecosystem Restoration (2021-2030). Here we opined various strategies for restoring degraded agroecosystems for sustainable development including the adoption of emerging paradigms such as micro-agriculture, urban agriculture, and landless agriculture for averting the mounting pressure on agroecosystems for the benefit of both people and the planet.