ABSTRACT
The CYP171 enzyme is known to catalyse a key step in the steroidogenesis of mammals. The substrates progesterone and pregnenolone are first hydroxylated at the C17 position, and this is followed by cleavage of the C17-C20 bond to yield important precursors for glucosteroids and androgens. In this study, we focused on the reaction of the bovine CYP17A1 enzyme with progesterone as a substrate. On the basis of a created homology model, active-site residues were identified and systematically mutated to alanine. In whole-cell biotransformations, the importance of the N202, R239, G297 and E305 residues for substrate conversion was confirmed. Additionally, mutation of the L206, V366 and V483 residues enhanced the formation of the 16α-hydroxyprogesterone side product up to 40 % of the total product formation. Furthermore, residue L105 was found not to be involved in this side activity, which contradicts a previous study with the human enzyme.
Subject(s)
Progesterone/metabolism , Steroid 17-alpha-Hydroxylase/metabolism , Animals , Catalytic Domain , Cattle , Hydroxyprogesterones/chemistry , Hydroxyprogesterones/metabolism , Models, Molecular , Mutagenesis, Site-Directed , Progesterone/chemistry , Stereoisomerism , Steroid 17-alpha-Hydroxylase/chemistry , Steroid 17-alpha-Hydroxylase/genetics , Substrate SpecificityABSTRACT
Rhizopus microsporus var. oligosporus is a fungus that belongs to the Mucoraceae family that is used for the preparation of some soy-fermented foods. Microbial biotransformation of progesterone by R. microsporus var. oligosporus afforded some monohydroxylated and dihydroxylated metabolites. The main product was purified using chromatographic methods and identified as 11α-hydroxyprogesterone on the basis of its spectroscopic features. Time course studies by high-performance thin-layer chromatography demonstrated that this fungi efficiently hydroxylated progesterone at the 11α-position for 3 days with a yield of 76.48%, but beyond this time, the microorganism transformed 11α-hydroxyprogesterone into dihydroxylated metabolites. 11α-Hydroxyprogesterone is widely used as a precursor in the synthesis of hydrocortisone and other steroidal anti-inflammatory agents.
Subject(s)
Hydroxyprogesterones/metabolism , Progesterone/metabolism , Rhizopus/metabolism , Biomass , Biotransformation , Chromatography, High Pressure Liquid , Hydroxylation , Hydroxyprogesterones/chemistry , Magnetic Resonance Spectroscopy , Progesterone/chemistry , Rhizopus/growth & developmentABSTRACT
Previous studies indicated that maturation inducing hormone, 17α, 20Ć-Dihydroxy-4-pregnen-3-one (DHP), probably through nuclear progestin receptor (Pgr), might be involved in spermatogenesis and oogenesis in fish. To further elucidate DHP actions in teleostean ovarian differentiation, we analyzed the expression of pgr in the ovary of Nile tilapia (Oreochromis niloticus), and performed RU486 (a synthetic Pgr antagonist) treatment in XX fish from 5 days after hatching (dah) to 120 dah. Tilapia Pgr was abundantly expressed in the follicular cells surrounding oocytes at 30 and 90 dah. Continuous RU486 treatment led to the blockage of oogenesis and masculinization of somatic cells in XX fish. Termination of RU486 treatment and maintenance in normal condition resulted in testicular differentiation, and estrogen compensation in RU486-treated XX fish successfully restored oogenesis. In RU486-treated XX fish, transcript levels of female dominant genes were significantly reduced, while male-biased genes were evidently augmented. Meanwhile, both germ cell mitotic and meiotic markers were substantially reduced. Consistently, estrogen production levels were significantly declined in RU486-treated XX fish. Taken together, our data further proved that DHP, possibly through Pgr, might be essential in the ovarian differentiation and estrogen production in fish.
Subject(s)
Cichlids/physiology , Ovary/cytology , Progestins/physiology , Receptors, Progesterone/antagonists & inhibitors , Animals , Cell Differentiation , Estrogens/chemistry , Female , Gene Expression Profiling , Hydroxyprogesterones/chemistry , Immunoenzyme Techniques , Immunohistochemistry , Male , Meiosis , Mifepristone/chemistry , Mitosis , Polymerase Chain Reaction , Sex Differentiation , SpermatogenesisABSTRACT
OBJECTIVE: 17-alpha hydroxyprogesterone caproate (17-OHPC) is available both as an Food and Drug Administration (FDA)-approved medication and as a product prepared for individual patients by compounding pharmacies. Compounding pharmacies may omit the preservative that is used in the FDA-approved formulation or use an alternate preservative and may dispense 17-OHPC in containers that differ from the FDA-approved product. The objective of this study was to assess the stability and the microbiologic and pyrogen status of 17-OHPC formulations under various compounding and dispensing conditions. STUDY DESIGN: 17-OHPC was prepared by a local compounding pharmacy. The formulations that were prepared included 1 identical to the FDA-approved product with benzyl alcohol as a preservative, 1 with benzalkonium chloride as a preservative, and 1 without a preservative. These various formulations were dispensed into either single-dose 1-mL plastic syringes or glass vials or 10-mL glass vials. The concentration of 17-OHPC and microbial and pyrogen status were evaluated at various time intervals over the ensuing 19 weeks. RESULTS: The concentration of 17-OHPC did not change over the duration of study, regardless of the dispensing medium that was used or the absence or presence of any preservatives. The preparations remained microbe- and pyrogen-free during the study period, regardless of the dispensing medium that was used or the absence of presence of any preservatives. CONCLUSION: Products that contained 17-OHPC tested in this study were quite stable over the 19-week period of study in different dispensing containers and in the absence or presence of a different preservative. The compounded products remained sterile and pyrogen-free during the period of observation.
Subject(s)
Drug Compounding/standards , Hydroxyprogesterones/standards , Preservatives, Pharmaceutical/standards , 17 alpha-Hydroxyprogesterone Caproate , Drug Contamination , Drug Stability , Endotoxins/analysis , Humans , Hydroxyprogesterones/chemistry , Hydroxyprogesterones/therapeutic use , Premature Birth/prevention & control , Pyrogens/analysis , Time Factors , United States , United States Food and Drug AdministrationABSTRACT
A two-step stereoselective preparation of a goldfish pheromone, 17α,20Ć-dihydroxy-4-pregnen-3-one, is reported from the readily available cortexolone in 64% overall yield. The (20S)-epimer was also synthesized in three steps from cortexolone with an overall yield of 47%. A microscale chiroptical technique based on a host/guest complexation mechanism between the substrate and a dimeric metalloporphyrin host (tweezer) was used to confirm the stereochemical assignment, while Density Functional Theory (DFT) calculations were employed to explain the high stereoselectivity induced by the 17α-hydroxyl and C18-methyl groups.
Subject(s)
Hydroxyprogesterones/chemical synthesis , Metalloporphyrins/chemistry , Optical Tweezers , Pheromones/chemistry , Quantum Theory , Animals , Circular Dichroism , Goldfish , Hydroxyprogesterones/chemistry , Molecular Structure , Optics and Photonics/instrumentation , Optics and Photonics/methods , Pheromones/chemical synthesis , StereoisomerismABSTRACT
A previous study identified the peroxisome proliferator-activated receptor alpha (PPARalpha) activation biomarkers 21-steroid carboxylic acids 11beta-hydroxy-3,20-dioxopregn-4-en-21-oic acid (HDOPA) and 11beta,20-dihydroxy-3-oxo-pregn-4-en-21-oic acid (DHOPA). In the present study, the molecular mechanism and the metabolic pathway of their production were determined. The PPARalpha-specific time-dependent increases in HDOPA and 20alpha-DHOPA paralleled the development of adrenal cortex hyperplasia, hypercortisolism, and spleen atrophy, which was attenuated in adrenalectomized mice. Wy-14,643 activation of PPARalpha induced hepatic FGF21, which caused increased neuropeptide Y and agouti-related protein mRNAs in the hypothalamus, stimulation of the agouti-related protein/neuropeptide Y neurons, and activation of the hypothalamic-pituitary-adrenal (HPA) axis, resulting in increased adrenal cortex hyperplasia and corticosterone production, revealing a link between PPARalpha and the HPA axis in controlling energy homeostasis and immune regulation. Corticosterone was demonstrated as the precursor of 21-carboxylic acids both in vivo and in vitro. Under PPARalpha activation, the classic reductive metabolic pathway of corticosterone was suppressed, whereas an alternative oxidative pathway was uncovered that leads to the sequential oxidation on carbon 21 resulting in HDOPA. The latter was then reduced to the end product 20alpha-DHOPA. Hepatic cytochromes P450, aldehyde dehydrogenase (ALDH3A2), and 21-hydroxysteroid dehydrogenase (AKR1C18) were found to be involved in this pathway. Activation of PPARalpha resulted in the induction of Aldh3a2 and Akr1c18, both of which were confirmed as target genes through introduction of promoter luciferase reporter constructs into mouse livers in vivo. This study underscores the power of mass spectrometry-based metabolomics combined with genomic and physiologic analyses in identifying downstream metabolic biomarkers and the corresponding upstream molecular mechanisms.
Subject(s)
Biomarkers/metabolism , Hydroxyprogesterones/metabolism , Hypothalamo-Hypophyseal System/physiology , PPAR alpha/metabolism , Pituitary-Adrenal System/physiology , Progestins/metabolism , Adrenal Cortex Hormones/metabolism , Adrenalectomy , Alcohol Oxidoreductases/metabolism , Aldehyde Oxidoreductases/genetics , Aldehyde Oxidoreductases/metabolism , Animals , Biomarkers/chemistry , Fasting , Hydroxyprogesterones/chemistry , Liver/metabolism , Male , Mass Spectrometry , Metabolomics , Mice , Mice, Inbred C57BL , Mice, Knockout , Molecular Structure , Oligonucleotide Array Sequence Analysis , Oxidation-Reduction , PPAR alpha/genetics , Peroxisome Proliferators/administration & dosage , Peroxisome Proliferators/metabolism , Progestins/chemistry , Pyrimidines/administration & dosage , Pyrimidines/metabolism , Urine/chemistryABSTRACT
The well-known phosphoramidite chemistry routinely used in solid-phase oligonucleotide synthesis is exploited here in the preparation of steroid conjugates in solution. The applicability of the method is tested by conjugating one nucleosidic and one non-nucleosidic phosphoramidite building block to 11-alpha-hydroxyprogesterone. The suitability of one of the conjugates synthesized (5) for a competitive binding assay is also demonstrated.
Subject(s)
Combinatorial Chemistry Techniques/methods , Hydroxyprogesterones/chemistry , Organophosphorus Compounds/chemistry , Hydroxyprogesterones/chemical synthesis , Organophosphorus Compounds/chemical synthesisABSTRACT
Recently, studies on the steroidal hormone activity in the brain have attracted attention, and the influences of the varied glucosides and their artificial derivatives have been discussed; additionally, it has been suggested that glucosides are the synthetic precursors of glucuronide as a label molecule. However, glucosides are formed with 11α-hydroxyprogesterone (1), which is important as a blood pressure regulator, but anti-androgen activity remains unknown. Using UDP-glucosyltransferase, glucoside synthesis was successful in linking Ć-d-glucopyranose and Ć-d-laminaribiose to 11α oxygen of 1 at a high conversion ratio, and full assignment structure was analyzed for the two glucosides by high-resolution quadrupole-time flight electrospray ionization-mass spectrometry, 1D (1H and 13C) NMR and 2D (COSY, ROESY, HSQC-DEPT and HMQC) NMR. Furthermore, the bioactivity of 1 and two 11α-hydroxyprogesterone glucosides [11α-(Ć-d-glucopyranosyl)oxyprogesterone, 2, and 11α-(Ć-d-laminaribiosyl)oxyprogesterone, 3] was tested in vitro. On rotenone-induced PC12 cells, the two 11α-hydroxyprogesterone glucosides (2 and 3) showed superior neuroprotective effects and increased cellular ATP levels compared with those of 1.
Subject(s)
Glucosides/chemistry , Glucosyltransferases/metabolism , Hydroxyprogesterones/chemistry , Hydroxyprogesterones/metabolism , Neuroprotective Agents/chemistry , Neuroprotective Agents/metabolism , Animals , Biotransformation , Hydroxyprogesterones/pharmacology , Neuroprotective Agents/pharmacology , PC12 Cells , RatsSubject(s)
Hydroxyprogesterones/chemistry , Premature Birth/prevention & control , Progesterone/chemistry , Progestins/chemistry , 17 alpha-Hydroxyprogesterone Caproate , Animals , Female , Humans , Hydroxyprogesterones/adverse effects , Hydroxyprogesterones/therapeutic use , Mice , Obstetrics , Pregnancy , Progesterone/adverse effects , Progesterone/pharmacology , Progesterone/therapeutic use , Progestins/adverse effects , Progestins/pharmacology , Progestins/therapeutic use , Terminology as TopicABSTRACT
OBJECTIVE: To study the chemical constituents in the aerial part of Reineckea carnea. METHOD: The compounds were isolated by extraction, silica gel, gel, and reversed-phase silica gel coloum chromatography, and high-performance liquid chromatography. The structures were identified by various spectroscopic methods including 1D and 2D NMR spectrum, MS, IR, etc. RESULT: Six compounds were isolated and identified as 1alpha, 3beta-dihydroxy-5beta-pregn-16-en-20-one-3-O-beta-D-glucopyranoside (1), syringaresinol-beta-D-glucoside (2), sophoraflavone B (3), stigmast-5, 22-dien-3-O-beta-D-glucopyranoside (4), daucosterol (5), a-D-glucose (6). CONCLUSION: Compound 1 was a new compound, coumpounds 2-6 were obtained from the plant for the first time.
Subject(s)
Drugs, Chinese Herbal/chemistry , Magnoliopsida/chemistry , Plant Components, Aerial/chemistry , Chromatography, High Pressure Liquid , Flavanones/chemistry , Glucose/chemistry , Glucosides/chemistry , Hydroxyprogesterones/chemistry , Lignans/chemistry , Magnetic Resonance Spectroscopy , Molecular Structure , Sitosterols/chemistry , Spectrometry, Mass, Electrospray IonizationABSTRACT
The continuous ethyl acetate extraction of progesterone and 11alpha-hydroxyprogesterone, a reactant and the product of the biotransformation step involved in corticosteroid production, was studied in a microchannel at different flow velocities. In addition, non-steady state batch extraction without mixing was performed and modelled in order to verify the theoretically predicted parameters. In order to analyze experimental data and to forecast microreactor performance, a three-dimensional mathematical model with convection and diffusion terms was developed considering the velocity profile for laminar flow of two parallel phases in a microchannel at steady-state conditions. For the numerical solution of a complex equation system, non-equidistant finite differences were used. Very good agreement between model calculations and experimental data was achieved without any fitting procedure. Due to the efficient phase separation and high extraction yields obtained, the micro scale extraction units were found to be a promising tool for the development of an integrated system of 11alpha-hydroxylation of progesterone by Rhizopus nigricans in the form of pellets.
Subject(s)
Hydroxyprogesterones/chemistry , Progesterone/chemistry , Steroids/chemistry , Acetates/chemistry , Adrenal Cortex Hormones , Algorithms , Biophysics/methods , Computer Simulation , Diffusion , Equipment Design , Models, Statistical , Models, Theoretical , Rhizopus/metabolism , Temperature , Water/chemistryABSTRACT
A systematic polymorph screening process was conducted on the steroid hydroxyprogesterone caproate, which had only one previously described orthorhombic crystalline form (A), in order to fully elucidate its solid state properties. Cooling, anti-solvent and evaporative techniques largely reproduced the same polymorph, but slurries in various solvents over two days produced a new triclinic form (B). Experiments at different temperatures in ethyl acetate or isopropyl alcohol confirmed this was an enantiotropic system with a transition temperature of approximately 30Ā°C. DSC was used to confirm the transition of Form B to Form A below the melting point. Form B was the thermodynamically stable form at room temperature, and 8% less soluble in a non-aqueous solvent mixture. In viscous solvents used commercially to dissolve the oil-soluble steroid for injection, solutions near the solubility limit can remain supersaturated after exposure to cooler temperatures for months. In resolving the crystalline structure of Form A, a third conformational polymorph was detected that exists only at -133 to -143Ā°C; this monoclinic form was designated Form C, and converts back to Form A upon warming to room temperature. These studies have increased the understanding of this drug and how the polymorphs may affect its physical stability in different dosage forms.
Subject(s)
Crystallization , Hydroxyprogesterones/chemistry , 17 alpha-Hydroxyprogesterone Caproate , Calorimetry, Differential Scanning , Solubility , TemperatureABSTRACT
Chemical modification of progesterone molecule leads to changes both in the gestagenic activity of new derivatives and in their specific binding with progesterone receptors. The passage from esters (acetomepregenole, butagest) to the corresponding OH-forms such as 17a-acetoxy-3b-hydroxy-6-methyl-pregna-4,6-dien-20-one (ABMP)is accompanied by an increase in the binding with progesterone receptors in vitro. The translocation of a double bond from endocyclic (N6-N7) to exocyclic position (methylene group at N6 in ABMP) has no significant effect on the ability to binding with progesterone receptors.
Subject(s)
Endometrium/metabolism , Progesterone Congeners/metabolism , Progestins/metabolism , Receptors, Progesterone/metabolism , 17-alpha-Hydroxyprogesterone/analogs & derivatives , 17-alpha-Hydroxyprogesterone/chemistry , 17-alpha-Hydroxyprogesterone/metabolism , Adult , Endometrial Hyperplasia/metabolism , Endometrial Hyperplasia/prevention & control , Female , Humans , Hydroxyprogesterones/chemistry , Hydroxyprogesterones/metabolism , Middle Aged , Pregnadienediols/chemistry , Pregnadienediols/metabolism , Pregnenes/chemistry , Pregnenes/metabolism , Progesterone Congeners/chemistry , Progestins/chemistry , Structure-Activity RelationshipABSTRACT
The fluorescence behaviour of 4,6,8(14)-trien-3-one steroids, which exhibit fluorescence in protic media but no fluorescence in hydrophobic environments, was used to characterize the molecular nature and temperature-sensitivity of steroid hormone-biomembrane interactions. Since 17 alpha-hydroxyprogesterone as the key intermediate is known to accumulate in smooth endoplasmic reticulum membranes in the course of adrenal and testicular steroid hormone biosynthesis, its fluorescent analogue, 6,8(14)-bis-dehydro-17 alpha-hydroxyprogesterone (BDHP), was used as the probe molecule. With rat testis microsomal membranes and liposomes, fluorescence quenching in the presence of membranes (related to fluorescence in aqueous solution) was independent on steroid concentration but was dependent on membrane lipid concentration in terms of a hyperbolic function. Complete fluorescence loss occurred at infinite lipid concentration at 20 degrees C, indicating complete insertion of the steroid probe into the hydrophobic portion of the membrane compartment. The partition coefficient KP increased with increasing temperature as a consequence of increased membrane fluidity. The result that BDHP fluorescence decreased considerably with elevated temperature in both the aqueous and the membrane milieu was interpreted as the consequence of increasing molecular mobility; this effect was much more pronounced in the aqueous than in the membrane environment. On the basis of local BDHP concentrations within the membrane phase (calculated from KP), relative fluorescence quenching was over-proportional at low temperatures; under that condition, hydrophobic interactions with rigid membrane lipid domains are obviously favoured.
Subject(s)
Endoplasmic Reticulum/chemistry , Fluorescent Dyes , Hydroxyprogesterones/chemistry , Intracellular Membranes/chemistry , Pregnatrienes , 17-alpha-Hydroxyprogesterone , Animals , Male , Rats , Spectrometry, Fluorescence , Temperature , Testis/chemistryABSTRACT
The natural steroid 11beta-hydroxyprogesterone is not only a modulator of 11beta-hydroxy-steroid dehydrogenase activity, but also an efficient inducer of tyrosine aminotransferase activity in hepatocytes. In contrast with the low affinity for the mineralocorticoid receptor. 11beta-hydroxyprogesterone binds well to both the glucocorticoid receptor and the carrier protein transcortin. It is accepted that the introduction of a 1:ene double bond into 3-keto 4:ene steroids increases the glucocorticoid potency, so that 3-keto-1,4:diene steroids show improved chemical stability and are more potent glucocorticoids than their respective 4:ene analogs. The steroid pregna-1,4-diene-11beta-ol-3,20-dione (deltaHOP) had previously been described as an anti-inflamatory compound and an inhibitor of macromolecular biosynthesis in thymocytes and lymphocytes. In such studies, deltaHOP also exhibited some particular glucocorticoid properties which made it attractive as a tool for the study of the mechanism of action of glucocorticoids. In the present paper we show that deltaHOP possesses some classical biological actions of glucocorticoids such as deposition of glycogen in rat liver, induction of TAT activity in hepatocytes, and inhibition of the uptake of leucine and thymidine by thymocytes. It also exhibits minimal sodium-retaining properties. Consistent with these biological effects, deltaHOP shows a 70 times lower relative binding affinity for the mineralocortioid receptor than aldosterone, but a reasonable affinity for the glucocorticoid receptor, and is as efficient as dexamethasone in dissociating the 90 kDa heat shock protein from the glucocorticoid receptor heterocomplex. However, the inhibition of the uptake of amino acids and nucleotides observed in the presence of deltaHOP is not efficiently blocked when thymocytes are coincubated in the presence of steroids with known antiglucocorticoid activity. deltaHOP is similarly inefficient in inducing chloramphenicol-acetyl transferase activity in cells transfected with a plasmid that possesses two canonical glucocorticoid-responsive elements. Unlike most glucocorticoids, deltaHOP does not induce the fragmentation of DNA in a regular pattern characteristic of apoptosis and it does not reduce thymus weight. This unusual dissociation of glucocorticoid parameters makes deltaHOP a useful tool to discriminate between mechanisms of action by which steroids can exert their biological effects.
Subject(s)
Glucocorticoids/metabolism , Hydroxyprogesterones/metabolism , Receptors, Glucocorticoid/metabolism , Animals , Apoptosis/drug effects , Chloramphenicol O-Acetyltransferase/biosynthesis , Chloramphenicol O-Acetyltransferase/genetics , Genes, Reporter , Glucocorticoids/chemistry , Glucocorticoids/pharmacology , HSP90 Heat-Shock Proteins/metabolism , Hydroxyprogesterones/chemistry , Hydroxyprogesterones/pharmacology , Kidney/drug effects , Kidney/metabolism , Kinetics , Liver/drug effects , Liver/metabolism , Male , Models, Molecular , Molecular Structure , Rats , Rats, Sprague-Dawley , Thymus Gland/drug effects , Thymus Gland/metabolism , TransfectionABSTRACT
Microbial hydroxylation of progesterone occurred in the culture of Acremonium strictum PTCC 5282 to produce two hydroxylated pregnene-like steroids. The metabolites were purified and characterized using spectroscopic methods and identified as 15alpha-hydroxyprogesterone and 15alpha-hydroxydeoxycorticosterone.
Subject(s)
Acremonium/metabolism , Progesterone/metabolism , Biotransformation , Hydroxycorticosteroids/chemistry , Hydroxycorticosteroids/metabolism , Hydroxylation , Hydroxyprogesterones/chemistry , Hydroxyprogesterones/metabolism , Magnetic Resonance Spectroscopy , Progesterone/chemistryABSTRACT
A novel functional group transfer reaction in 16 alpha,17 alpha-epoxy-3 beta-hydroxypregn-5-en-20-one by treatment with dry HCl affords 21-chloro-3 beta-hydroxy-pregn-5,16-dien-20-one, which has been utilized to obtain a number of C21-substituted derivatives.
Subject(s)
Hydroxyprogesterones/chemistry , Steroids/chemistry , Epoxy Compounds/chemical synthesis , Epoxy Compounds/chemistry , Hydroxyprogesterones/chemical synthesis , Steroids/chemical synthesisABSTRACT
We describe the synthesis of 13 beta- and 13 alpha-H-18-nor-androst-4-ene-3,17-dione (1a and 1b) from 18-hydroxyprogesterone (18-->20) hemiketal, via the 18-acetoxy-17 beta-hydroxyandrost-4-en-3-one formed by a modified Baeyer-Villiger reaction. Saponification of 18-acetoxyandrost-4-ene-3,17-dione with sonication, then retroaldolization in the presence of a formaldehyde trap, methone, afforded the mixture of 1a and 1b with 80% yield in a "one-pot" procedure and at room temperature. This yield was greatly improved, compared with the already published procedure.
Subject(s)
Hydroxyprogesterones/chemistry , Norandrostanes/chemical synthesis , Hydrogen Peroxide , Magnetic Resonance Spectroscopy , Mass Spectrometry , Molecular Structure , SonicationABSTRACT
Problems of cross-peak overlap in two-dimensional 1H homonuclear shift-correlated (COSY) spectra of steroids can often be avoided by use of the omega 1-decoupled COSY (COSYDEC) method. The selection of experimental parameters is discussed, and COSYDEC spectra are illustrated for 17a-oxa-D-homoandrost-4-ene-3,17-dione (testololactone), testosterone, and 17 alpha-hydroxyprogesterone. In a good case, a COSYDEC spectrum obtained at 250 MHz allows cross-peak recognition and assignment with facility comparable to that available only at 500 MHz for normal COSY spectra.
Subject(s)
Hydroxyprogesterones/chemistry , Magnetic Resonance Spectroscopy/methods , Testolactone/chemistry , Testosterone/chemistry , 17-alpha-Hydroxyprogesterone , Molecular StructureABSTRACT
18-Hydroxy-6,19-oxidoprogesterone and 18-hydroxy-11, 19-oxidoprogesterone were synthesized from readily available materials. The functionalization of C-18 was accomplished with phenyliodosodiacetate/iodine, whereas that of C-19 was carried out with the mercuric oxide/iodine system, both under irradiation with visible light. For 18-hydroxy-11,19-oxidoprogesterone, C-19 was functionalized before C-18, whereas the reverse order had to be used for the 6,19-oxido derivative.