RESUMEN
Molecular cloning of the human fibroblast Ca2+ channel pore-forming alpha1C subunit revealed (Soldatov, 1992. Proc. Natl. Acad. Sci. USA 89:4628-4632) a naturally occurring mutation g2254 --> a that causes the replacement of the conservative alanine for threonine at the position 752 at the cytoplasmic end of transmembrane segment IIS6. Using stably transfected HEK293 cell lines, we have compared electrophysiological properties of the conventional alpha(1C,77) human recombinant L-type Ca2+ channel with those of its mutated isoform alpha(1C,94) containing the A752T replacement. Comparative quantification of steady-state availability of the current carried by alpha(1C,94) and alpha(1C,77) showed that A752T mutation prevented a large (approximately 25%) fraction of the current carried by Ca2+ or Ba2+ from fully inactivating. This mutation, however, did not appear to alter significantly the Ca2+-dependence and kinetics of decay of the inactivating fraction of the current or its voltage-dependence. The data suggests that Ala752 at the cytoplasmic end of IIS6 might serve as a molecular determinant of the Ca2+ channel inactivation, possibly regulating the voltage-dependence of its availability.
Asunto(s)
Canales de Calcio Tipo L/fisiología , Alanina , Sustitución de Aminoácidos , Canales de Calcio Tipo L/química , Línea Celular , Clonación Molecular , Fibroblastos/fisiología , Humanos , Riñón , Potenciales de la Membrana/fisiología , Mutación Puntual , Proteínas Recombinantes/antagonistas & inhibidores , Proteínas Recombinantes/química , Treonina , TransfecciónRESUMEN
Lifetimes of states in 150Nd were measured using the recoil distance method following Coulomb excitation of 150Nd by a 132 MeV 32S beam. The experiment was performed at the Yale Tandem accelerator, employing the SPEEDY gamma-ray detector array and the New Yale Plunger Device. Reduced transition probabilities in 150Nd are compared to the predictions of the critical point symmetry X(5) of the phase/shape transition that occurs for the N = 90 rare earth isotones. Very good agreement was observed between the parameter-free (apart from scale) X(5) predictions and the low-spin level scheme of 150Nd, revealing this as the best case thus far for the realization of the X(5) symmetry.