RESUMEN
Leaf rust (Puccinia triticina Eriks) is a wheat disease causing substantial yield losses in wheat production globally. The identification of genetic resources with permanently effective resistance genes and the generation of mutant lines showing increased levels of resistance allow the efficient incorporation of these target genes into germplasm pools by marker-assisted breeding. In this study, new mutant (M3 generation) lines generated from the rust-resistant variety Kazakhstanskaya-19 were developed using gamma-induced mutagenesis through 300-, 350-, and 400-Gy doses. In field trials after leaf rust inoculation, 75 mutant lines showed adult plant resistance. These lines were evaluated for resistance at the seedling stage via microscopy in greenhouse experiments. Most of these lines (89.33%) were characterized as resistant at both developmental stages. Hyperspectral imaging analysis indicated that infected leaves of wheat genotypes showed increased relative reflectance in visible and near-infrared light compared to the non-infected genotypes, with peak means at 462 and 644 nm, and 1936 and 2392 nm, respectively. Five spectral indexes, including red edge normalized difference vegetation index (RNDVI), structure-insensitive pigment index (SIPI), ratio vegetation index (RVSI), water index (WI), and normalized difference water index (NDWI), demonstrated significant potential for determining disease severity at the seedling stage. The most significant differences in reflectance between susceptible and resistant mutant lines appeared at 694.57 and 987.51 nm. The mutant lines developed were also used for the development and validation of KASP markers for leaf rust resistance genes Lr1, Lr2a, Lr3, Lr9, Lr10, and Lr17. The mutant lines had high frequencies of "a" resistance alleles (0.88) in all six Lr genes, which were significantly associated with seedling resistance and suggest the potential of favorable haplotype introgression through functional markers. Nine mutant lines characterized by the presence of "b" alleles in Lr9 and Lr10-except for one line with allele "a" in Lr9 and three mutant lines with allele "a" in Lr10-showed the progressive development of fungal haustorial mother cells 72 h after inoculation. One line from 300-Gy-dosed mutant germplasm with "b" alleles in Lr1, Lr2a, Lr10, and Lr17 and "a" alleles in Lr3 and Lr9 was characterized as resistant based on the low number of haustorial mother cells, suggesting the contribution of the "a" alleles of Lr3 and Lr9.
RESUMEN
The aim of this paper was to study the effect of plant growth regulators on callus induction and in vitro morphogenesis using various explants of Paulownia tomentosa to develop an efficient plant regeneration protocol. Different plant organ sections (leaves, apical shoot tips, petals, nodes, and internodes) were cultured as explants to identify the best in vitro explants responsive to callus induction and plant regeneration. Explants were cultivated on MS media supplemented with different concentrations of plant growth regulators (TDZ (Thidiazuron), BAP (6-Benzylaminopurine), kinetin, and NAA (1-Naphthaleneacetic acid). It was discovered that the addition of TDZ and NAA stimulated the induction of somatic embryogenesis. It was discovered that the MS medium with the combination of plant growth regulators BAP (35.5 µM) and NAA (5.4 µM) with the addition of 30.0 g/L maltose, 500.0 mg/L casein hydrolysate, and 250.0 mg/L L-proline was optimal for callus induction and multiple plant regeneration. The study of the regenerative capacity of various explants of Paulownia tomentosa in vitro showed that plant regeneration depends on the type of explant, and occurs in both ways, indirectly, through the formation of callus tissues and directly on the explant, without callus formation. As a result of this study, the efficient reproducible protocol of embryogenic callus formation and multiple shoot induction in vitro of Paulownia tomentosa was developed. This system provides a clear increase in the frequency of plant regeneration from 36.3 ± 3.4% to 38.6 ± 2.3% per embryogenic callus from leaves and apical shoot tips, respectively.
RESUMEN
Due to the environment pollution by cadmium (Cd) near industrial metallurgic factories and the widespread use of phosphorus fertilizers, the problem of toxic Cd effect on plants is well discussed by many authors, but the phytotoxicity of Cd under iron (Fe) deficiency stress has not been sufficiently studied. The aim of the work was to study comprehensively the effect of Cd under Fe deficiency conditions on physiological, biochemical, and anatomical parameters of rice varieties, to identify varietal differences in plant response to the effect of double stress. Relative resistance and sensitivity to the joint effect of Cd and Fe deficiency stress rice varieties have been identified. Double stress decreased a linear growth and biomass accumulation of roots and shoots (by 36-50% and 33-46% and 32-56% and 32-48%, accordingly), content of photosynthetic pigments (Chla, Chlb, and carotenoids by 36-51%, 32-47%, and 64-78%, accordingly), and relative water content (by 18-26%). Proline content increased by 28-103% in all rice varieties, but to a lesser extent in sensitive varieties. The thickness of the lower and upper epidermis and the diameter of vascular bundles of leaves decreased by 18-50%, 46-60%, and 13-48%, accordingly. The thickness of the root endodermis and exodermis and diameter of the central cylinder mainly decreased. The thickness of the exodermis increased slightly by 7%, and the diameter of the central cylinder remained at the control level in resistant Madina variety while in sensitive Chapsari variety, these indicators decreased significantly by 50 and 45%, accordingly. Thus, the aggravation of adverse effect of Cd under Fe deficiency conditions and the varietal specificity of plants' response to double stress were shown. It creates the need for further study of these rice varieties using Fe to identify mechanisms for reducing the toxic effect of Cd on plants as well as the study of Fe and Cd transporter genes at the molecular level.