Genomic comparison of chitinolytic enzyme systems from terrestrial and aquatic bacteria.

Chitin degradation ability is known for many aquatic and terrestrial bacterial species. However, differences in the composition of chitin resources between aquatic (mainly exoskeletons of crustaceans) and terrestrial (mainly fungal cell walls) habitats may have resulted in adaptation of chitinolytic enzyme systems to the prevalent resources. We screened publicly available terrestrial and aquatic chitinase-containing bacterial genomes for possible differences in the composition of their chitinolytic enzyme systems. The results show significant differences between terrestrial and aquatic bacterial genomes in the modular composition of chitinases (i.e. presence of different types of carbohydrate binding modules). Terrestrial Actinobacteria appear to be best adapted to use a wide variety of chitin resources as they have the highest number of chitinase genes, the highest diversity of associated carbohydrate-binding modules and the highest number of CBM33-type lytic polysaccharide monooxygenases. Actinobacteria do also have the highest fraction of genomes containing ß-1, 3-glucanases, enzymes that may reinforce the potential for degrading fungal cell walls. The fraction of bacterial chitinase-containing genomes encoding polyketide synthases was much higher for terrestrial bacteria than for aquatic ones supporting the idea that the combined production of antibiotics and cell-wall degrading chitinases can be an important strategy in antagonistic interactions with fungi.

BSA-Cu3(PO4)2 hybrid nanoflowers as a high-performance redox indicator for robust label-free electrochemical immunoassay.

In this work, we report that copper ion-centered hybrid nanoflowers (HNFs) can directly act as high-performance redox indicator via a facile one-step coprecipitation methodology. By altering the bovine serum albumin (BSA) concentration in the coprecipitation synthesis system, the nucleation, growth and electrochemical activities of BSA-Cu3(PO4)2 HNFs could be well regulated. Thanks to the intrinsic redox activity and high specific surface area of BSA-Cu3(PO4)2 HNFs, it could be utilized not only as the direct electrochemical signal indicator, but also acted as nanocarriers for loading numerous detection antibodies, which was further employed for robust label-free electrochemical immunoassay of α-fetoprotein (AFP). This assay evaded the fussy labeling procedure and the addition of extra redox-active substances in the test solutions, greatly improved the accuracy and stability of the immunosensor. The strategy sheds light on exploring the electrochemical redox indicator, with great adaptability for the routine biosensing application.

Lipids associated with plant-bacteria interaction identified using a metabolomics approach in an Arabidopsis thaliana model.

Background: Systemic acquired resistance (SAR) protects plants against a wide variety of pathogens. In recent decades, numerous studies have focused on the induction of SAR, but its molecular mechanisms remain largely unknown. Methods: We used a metabolomics approach based on ultra-high-performance liquid chromatographic (UPLC) and mass spectrometric (MS) techniques to identify SAR-related lipid metabolites in an Arabidopsis thaliana model. Multiple statistical analyses were used to identify the differentially regulated metabolites. Results: Numerous lipids were implicated as potential factors in both plant basal resistance and SAR; these include species of phosphatidic acid (PA), monogalactosyldiacylglycerol (MGDG), phosphatidylcholine (PC), phosphatidylethanolamine (PE), and triacylglycerol (TG). Conclusions: Our findings indicate that lipids accumulated in both local and systemic leaves, while other lipids only accumulated in local leaves or in systemic leaves. PA (16:0_18:2), PE (34:5) and PE (16:0_18:2) had higher levels in both local leaves inoculated with Psm ES4326 or Psm avrRpm1 and systemic leaves of the plants locally infected with Psm avrRpm1 or Psm ES4326. PC (32:5) had high levels in leaves inoculated with Psm ES4326. Other differentially regulated metabolites, including PA (18:2_18:2), PA (16:0_18:3), PA (18:3_18:2), PE (16:0_18:3), PE (16:1_16:1), PE (34:4) and TGs showed higher levels in systemic leaves of the plants locally infected with Psm avrRpm1 or Psm ES4326. These findings will help direct future studies on the molecular mechanisms of SAR.

Shift in rhizospheric and endophytic bacterial communities of tomato caused by salinity and grafting.

Saline water has to be used as an alternative resource in modern agriculture due to the increasing lack of fresh water. Approaches that promote the growth of crops under saline conditions have, therefore, become crucial. Grafting has been reported to be effective for this; however, the associated bacterial community remains unclear. To obtain a deeper understanding of the underlying microbial mechanisms, both grafted and non-grafted tomatoes were irrigated with three types of water having different electrical conductivity values. The experiment lasted 2.5 months, after which, the soil chemical properties and tomato heights were assessed. The rhizospheric and endophytic bacterial communities of samples from the different treatments were assessed by Illumina sequencing. The results showed that saline water significantly affected leaf-associated endophytic bacterial communities, whereas rhizosphere and root- and stem-associated bacterial communities were not affected. Increasing salinity increased the abundance of Gammaproteobacteria, but decreased the abundance of Actinobacteria, Alphaproteobacteria, Bacilli, and Acidobacteria at the class level of the leaf-associated bacterial community. Moreover, under higher salinity levels, grafting increased the diversity of the leaf-endophytic bacterial community. Overall, this study provides a comprehensive understanding of the rhizosphere and endophytic bacterial communities of tomato under saline conditions. The results highlight the importance of leaf-endophytic bacteria for salt response in plants. This is an important complementary finding to previous studies on the effect of salinity, which mainly focused on plant rhizosphere and root bacterial communities.

Using community analysis to explore bacterial indicators for disease suppression of tobacco bacterial wilt.

Although bacterial communities play important roles in the suppression of pathogenic diseases and crop production, little is known about the bacterial communities associated with bacterial wilt. Based on 16S rRNA gene sequencing, statistical analyses of microbial communities in disease-suppressive and disease-conducive soils from three districts during the vegetation period of tobacco showed that Proteobacteria was the dominant phylum, followed by Acidobacteria. Only samples from September were significantly correlated to disease factors. Fifteen indicators from taxa found in September (1 class, 2 orders, 3 families and 9 genera) were identified in the screen as being associated with disease suppression, and 10 of those were verified for potential disease suppression in March. Kaistobacter appeared to be the genus with the most potential for disease suppression. Elucidating microbially mediated natural disease suppression is fundamental to understanding microecosystem responses to sustainable farming and provides a possible approach for modeling disease-suppressive indicators. Here, using cluster analysis, MRPP testing, LEfSe and specific filters for a Venn diagram, we provide insight into identifying possible indicators of disease suppression of tobacco bacterial wilt.