How to repel a killer; chemical identification and effective repellent activity of commercial essential oils against kissing bugs.

Triatomines are haematophagous insects, some species are vectors of Trypanosoma cruzi, the aetiological agent of Chagas disease. The main strategy for interrupting T. cruzi transmission is to avoid contact of the vector populations with humans. Volatiles from commercial essential oils are excellent candidates to serve as repellents of kissing bugs. We used an exposure device to assess the repellence effect of eight commercial essential oils on Triatoma pallidipennis. The most effective oils were blended and evaluated against T. infestans, T. pallidipennis and Rhodnius prolixus. The blend was also evaluated on parasitised T. pallidipennis. Data were compared with the commercial repellent NN-diethyl-3-methylbenzamide. We recorded the time the insects spent in the proximity of the host and determined if any of the evaluated oils served as kissing bug repellent. We found commercial essential oils and a blend that significantly reduced the time spent in the proximity of the host. The blend was effective for use by human males and females, repelling infected and non-infected insects. The study of essential oils as repellents of blood-sucking disease-vector insects could shed light on the development of new control strategies.

Contamination effects on sexual selection in wild dung beetles.

Sexual selection influences the expression of secondary sexual traits, which are costly to produce and maintain and are thus considered honest indicators of individual condition. Therefore, sexual selection could select for high-quality individuals able to respond to stressful conditions, with impacts on population-level fitness. We sampled dung beetles from 19 pastures and investigated if contamination by herbicides and veterinary drugs modifies male investment in sexually selected traits and has associated population-level effects. We measured horn size, condition dependence (i.e. size-corrected body mass) and allometry, besides abundance and sexual size dimorphism in three species: Copris incertus, Euoniticellus intermedius and Digitonthophagus gazella. In contrary to our expectations, horn size was independent of contamination and individual condition. However, strong positive allometric relationships were reduced by herbicide contamination for C. incertus and D. gazella and were increased by ivermectin for C. incertus, revealing differential investment in horn production according to body size in contaminated habitats. At the population level, large-horned C. incertus males were more abundant in contaminated pastures, potentially revealing a case of evolutionary rescue by sexual selection or a plastic response to higher population densities. Finally, chemical compounds affected the sexual size dimorphism of all three species, with potential effects on female fecundity or intrasexual selection. Together, our findings indicate that contamination interferes with sexual selection processes in the wild, opening new questions regarding the role of sexual selection in favouring species persistence in contaminated environments.

When is a male too hot? Fitness outcomes when mating with high temperature, sick males.

Infection may cause some insects to increase their body temperature to deal against pathogens successfully. However, one unclear aspect is whether females may use male temperature to discriminate sick from healthy partners. We tested this by using Tenebrio molitor beetles whose females use the intensity of male antennal and leg stroking that take place during mating, to discriminate among partners. We predicted that females will not choose males that are too hot and/or will penalize these male partners in terms of a reduced egg number and hatching success. We manipulated males by infecting them with an entomopathogenic fungus, and exposed these males versus control males to females. Infected and non-infected males increased their temperature during copulation but females did not choose on the basis of male's temperature or infection status. However, females laid fewer eggs and/or had a reduced hatching success after mating with infected males that had a body temperature higher than ca. 24 °C. We postulate that male temperature is another source of information involved in postcopulatory mate choice that, along with courtship traits, compose a multimodal signaling system.

Insect thermal limits in warm and perturbed habitats: Dragonflies and damselflies as study cases.

Disturbance (e.g. loss of plant cover) increases ambient temperature which can be lethal for ectotherm insects especially in hot places. We compared the thorax temperatures of 26 odonate species as a function of body size, habitat quality ("conserved" and cooler vs "perturbed" and warmer) and suborder (Anisoptera vs Zygoptera), as well as critical thermal maximum (CTmax) and as a function of habitat quality in Argia pulla (Zygoptera) and Orthemis ferruginea (Anisoptera). We expected thorax temperatures to differ between suborders based on their differences in body size and habitat quality status, and that populations in perturbed sites would have higher critical thermal maxima compared to those in conserved sites. This study was done in a tropical region with high ambient temperatures. Anisopterans had a higher body temperature than zygopterans, with no difference between habitats. Thoracic and air temperature were positively related, yet body temperatures were higher than the ambient temperature. A. pulla had higher CTmax in the perturbed sites, while O. ferruginea showed the opposite trend. Microenvironmental changes increase the ambient temperature, perhaps filtering insect species. The apparent resilience of odonates to disturbance should be examined more closely (using more species), especially in small species like the zygopterans which appear to be more strongly affected by ambient temperature.

Knowledge and perceptions about Covid-19 in the marginalized population of southeastern Mexico.

Not available.

Why do bugs perish? Range size and local vulnerability traits as surrogates of Odonata extinction risk.

Despite claims of an insect decline worldwide, our understanding of extinction risk in insects is incomplete. Using bionomic data of all odonate (603 dragonflies and damselflies) North American species, we assessed (i) regional extinction risk and whether this is related to local extirpation; (ii) whether these two patterns are similar altitudinally and latitudinally; and (iii) the areas of conservation concern. We used geographic range size as a predictor of regional extinction risk and body size, thermal limits and habitat association as predictors of local extirpation. We found that (i) greater regional extinction risk is related to narrow thermal limits, lotic habitat use and large body size (this in damselflies but not dragonflies); (ii) southern species are more climate tolerant but with more limited geographic range size than northern species; and (iii) two priority areas for odonate conservation are the cold temperate to sub-boreal northeastern USA and the transversal neo-volcanic system. Our approach can be used to estimate insect extinction risk as it compensates for the lack of abundance data.

Coinfection by Trypanosoma cruzi and a fungal pathogen increases survival of Chagasic bugs: advice against a fungal control strategy.

Triatomine bugs carry the parasitic protozoa Trypanosoma cruzi, the causal agent of Chagas disease. It is known that both the parasite and entomopathogenic fungi can decrease bug survival, but the combined effect of both pathogens is not known, which is relevant for biological control purposes. Herein, the survival of the triatomine Meccus pallidipennis (Stal, 1872) was compared when it was coinfected with the fungus Metarhizium anisopliae (Metschnikoff) and T. cruzi, and when both pathogens acted separately. The immune response of the insect was also studied, using phenoloxidase activity in the bug gut and hemolymph, to understand our survival results. Contrary to expectations, triatomine survival was higher in multiple than in single challenges, even though the immune response was lower in cases of multiple infection. We postulate that T. cruzi exerts a protective effect and/or that the insect reduced the resources allocated to defend itself against both pathogens. Based on the present results, the use of M. anisopliae as a control agent should be re-considered.

The cost of being a killer's accomplice: Trypanosoma cruzi impairs the fitness of kissing bugs.

Relatively little is known about the fitness effects and life history trade-offs in medically important parasites and their insect vectors. One such case is the triatomine bugs and the parasite Trypanosoma cruzi, the key actors in Chagas disease. Previous studies have revealed some costs but have not simultaneously examined traits related to development, reproduction, and survival or their possible trade-offs. In addition, these studies have not compared the effects of genetically different T. cruzi strains that differ in their weakening effects in their vertebrate hosts. We compared the body size of the bugs after infection, the number of eggs laid, hatching/non-hatching rate, hatching success, survival, and the resulting number of parasites in Meccus (Triatoma) pallidipennis bugs that were experimentally infected with two strains of T. cruzi (Chilpancingo [CH], the most debilitating in vertebrates; and Morelos [MO], the least debilitating) (both belonging to TcI group). Our results showed that infection affects size (MO < CH; MO and CH = control), number of eggs laid (MO and CH < control) hatching/non-hatching rate (MO < control < CH), hatching success (control < MO, CH = control = MO), and survival (Chilpancingo < Morelos < control). In addition, the CH strain produced more parasites than the MO strain. These results suggest that (a) infection costs depend on the parasite's origin, (b) the more debilitating effects of the CH strain are due to its increased proliferation in the host, and (c) differences in pathogenicity among T. cruzi strains can be maintained through their different effects on hosts' life history traits. Probably, the vectorial capacity mediated by a more aggressive strain could be reduced due to its costs on the triatomine, leading to a lower risk of vertebrate and invertebrate infection in natural populations.

Damselfly (Odonata: Calopterygidae) Population Decline in an Urbanizing Watershed.

Reduction of terrestrial vegetation and degradation of water quality are among the factors driving insect population decline in growing cities. In this study, we investigated the extent of habitat deterioration, behavioral and physiological responses, and fitness of a damselfly [Hetaerina americana (F.)] population in a semitropical region in central Mexico. The study population was located in a riverine area that crosses a small urban area (Tehuixtla city). We related two habitat variables (tree/shrub covered area and numbers of wastewater outlets) to presumable damselfly responses (larval and adult abundance, duration of adults exposed directly to sunlight, lipid content and muscle mass, and egg survival) over the years 2002 and 2016. We detected a reduction in terrestrial vegetation cover, an increase in wastewater outlets, and a decrease in larval and adult abundance. Adults were more exposed to sunlight in 2016 than in 2002 and showed a reduced lipid content and muscle mass in 2016. Egg survival also decreased. Although correlative, these results suggest impairment of damselfly condition (via lipid and muscle reduction) and fitness as urbanization increases.

New Entamoeba group in howler monkeys (Alouatta spp.) associated with parasites of reptiles.

Our knowledge of the parasite species present in wildlife hosts is incomplete. Protozoans such as amoebae of the genus Entamoeba infect a large variety of vertebrate species, including NHPs. However, traditionally, their identification has been accomplished through microscopic evaluation; therefore, amoeba species have not always been identified correctly. We searched for Entamoeba spp. using a fragment of the small subunit rDNA in free-ranging howler monkeys (Alouatta palliata and A. pigra) from southeast Mexico. One hundred fifty five samples were collected, with 46 from A. palliata and 109 from A. pigra and 8 of the total samples were positive. We detected a new clade of Entamoeba, which was separated from other described species but closer to E. insolita, as well as an unnamed sequence typically found in iguana species with low shared identity values (<90%). We designated this new clade as conditional lineage 8 (CL8) and we have shown that members of this group are not exclusive to reptiles.

Odonata (dragonflies and damselflies) as a bridge between ecology and evolutionary genomics.

Odonata (dragonflies and damselflies) present an unparalleled insect model to integrate evolutionary genomics with ecology for the study of insect evolution. Key features of Odonata include their ancient phylogenetic position, extensive phenotypic and ecological diversity, several unique evolutionary innovations, ease of study in the wild and usefulness as bioindicators for freshwater ecosystems worldwide. In this review, we synthesize studies on the evolution, ecology and physiology of odonates, highlighting those areas where the integration of ecology with genomics would yield significant insights into the evolutionary processes that would not be gained easily by working on other animal groups. We argue that the unique features of this group combined with their complex life cycle, flight behaviour, diversity in ecological niches and their sensitivity to anthropogenic change make odonates a promising and fruitful taxon for genomics focused research. Future areas of research that deserve increased attention are also briefly outlined.

Chemical symphony: Unraveling triatomine aggregation and attraction patterns for innovative pest control solutions.

Aggregation is a spatial distribution pattern where individuals can be grouped through interaction with particular signals or cues [e.g., chemical substances]. Numerous triatomine species exhibit attraction and aggregation around both conspecific and heterospecific feces. It remains unclear whether compounds released by feces function as signals (pheromones or synomones) or cues. Employing a bioassay that mimics field conditions, we investigated the response of Triatoma pallidipennis bugs to volatiles present in its feces and blends of these compounds. Our findings indicate that short-chain aldehydes, namely nonanal, octanal, heptanal, and hexanal, attract fifth-instar nymphs. Furthermore, insects responded to individual compounds as well as secondary, ternary, and quaternary blends. The most attractive blend consisted of all four aldehydes. Additionally, quaternary blends at various compound ratios attracted fifth-instar nymphs of T. phyllosoma and T. longipennis. We discuss the potential roles of these compounds as signals or cues and explore their application as bait in control programs.

Merging socioecological variables to predict risk of Chagas disease.

How far are we from predicting the occurrence of zoonotic diseases? In this paper we have made use of both socioecological and ecological variables to predict Chagas disease occurrence. Chagas disease involves, Trypanosoma cruzi, a complex life-cycle parasite which requires two hosts: blood-feeding triatomine insects and vertebrate hosts including humans. We have used a common risk assessment method combined with datasets that imply critical environmental and socioeconomic drivers of Chagas dynamics to predict the occurrence of this disease. We also carried out a network analysis to assess the interactions among triatomines and mammal host species given their human contact via whether hunted, domesticated or associated with anthropogenic landscapes in Mexico. We found that social backwardness variation, lack of health services and altitude had the largest relative influence Chagas events. Triatoma pallidipennis made use of the largest host diversity. Host species shared by the highest number of different triatomines were a woodrat, the highly appreciated bushmeat, and racoon. These results indicate both the predominance of socio-economic factors over ecological ones, and how close we are from predicting zoonotic diseases.

Vector mosquito distribution and richness are predicted by socio-economic, and ecological variables.

Mosquitoes of vectorial importance represent a ubiquitous and constant threat of potentially devastating arboviral outbreaks. Our ability to predict such outcomes is still restricted. To answer this, we have used an extensive data collection of 23 vector and 233 non-vector mosquito species distributed throughout the Mexican territory and linked them to social and environmental factors. Our aim was to predict vector and non-vector mosquitoes' distribution and species richness based on socioeconomic and environmental data. We found that lack of health services, human population variation, ecological degradation, and urban-rural categorization contributed significantly to explain the distribution of vector mosquitoes. mosquitoes. This phenomenon is probably attributed to the degradation of natural ecosystems as it creates favorable conditions for the proliferation of vector mosquitoes. The richness of vector mosquitoes was similarly explained by most of these variables as well as altitude. As for non-vector mosquitoes, social marginalization, ecological degradation, anthropogenic impact, and altitude explain species richness and distribution. These findings illustrate the complex interaction of environmental and socioeconomic factors behind the distribution of mosquitoes, and the potential for arboviral disease outbreaks. Areas with human populations at highest risk for mosquito-borne diseases should be primary targets for vector control.

Lessons to be popular: the chemical basis of aggregation in Trypanosoma cruzi-infected and non-infected Chagasic bugs.

Aggregation is one of the most remarkable behaviours in the animal kingdom-a process that is usually governed by pheromones. Triatomines are blood-sucking bugs that act as vectors of Trypanosoma cruzi, the etiological agent of Chagas disease in mammals, including humans. Triatomines usually gather in roosting refuges by using aggregation pheromones of unknown chemical structure. In terms of vector control, one option to reduce triatomine-human contact is via capturing the insects into traps baited with lures based on such aggregation pheromones. As a first step towards this aim, we elucidated the aggregation pheromone in the triatomine Triatoma pallidipennis, using T. cruzi-infected and non-infected bugs. We used different extraction techniques and gas chromatography coupled to mass spectrometry for the identification. Also, two different bioassays were implemented for evaluating the attractant and arrestant activity of the pheromone. We found that T. pallidipennis produced short-chain aldehydes as attractants, and nitrogen-derived compounds as arrestants. We detected differences in the production and perception of these compounds according to whether animals were infected or not. These findings show that T. cruzi may influence triatomine chemical ecology and are promising tools for triatomine control.

Thinking green: Insecticidal effect of biorational solutions against Triatoma pallidipennis Stal (Hemiptera: Triatominae).

The control of triatomine vectors depends almost exclusively on conventional insecticides. These compounds can, nevertheless, cause negative effects on environmental and human health as well as induce resistance in triatomines. Therefore, we need to look for more sustainable alternatives. Triatoma pallidipennis is one of the main chagasic vectors in Mexico. We evaluated the insecticidal effectiveness of two oils (neem and cinnamon), and two desiccants (potassium salts of fatty acids and diatomaceous earth), on 3rd instar nymphs of T. pallidipennis. The laboratory test involved direct exposure of the treatments to the insects. We found that diatomaceous earths caused 80 % mortality of nymphs after 30 days. Meanwhile, the cumulative mortality for the other treatments did not exceed 50 %. When applied to inert surfaces, the powder formulation of diatomaceous earth demonstrated greater effectiveness than the aqueous suspension. Thus, diatomaceous earth could be a promising alternative for an environmentally friendly control of triatomines.

Copulatory behavior in a pholcid spider: males use specialized genitalic movements for sperm removal and copulatory courtship.

Sexual selection may operate on pre-copulatory, copulatory, and post-copulatory traits. An example of a copulatory target of sexual selection is the genitalic movements a male performs during copulation. These movements may function either to prevent sperm competition or to influence a female's fertilization decision. Here we investigated how copulation duration, pedipalp movements, and abdominal movements that males of the pholcid spider Holocnemus pluchei produce during copulation influence sperm removal and/or patterns of successful sperm transfer. We compared mating events with virgin and mated females for differences in copulatory and post-copulatory behavior. We expected longer copulation duration, longer pedipalp movement duration, and more complex and frequent pedipalp and abdominal movements when males mated with mated females compared to virgin females. Except for abdominal movements, our results corroborated these predictions. Furthermore, when we investigated mating events with mated females, we observed sperm mass ejection from the female gonopore and physical removal of sperm by males' procursi. Females with interrupted second mating events showed a significant reduction of stored sperm masses compared to females with completed mating events. We suggest that males use alternating pedipalp movements to remove most of the rival sperm stored by mated females prior to sperm transfer. Copulation duration and pedipalp movements can be further used to transfer sperm and/or as a form of genitalic copulatory courtship.

Evolutionary ecology of Odonata: a complex life cycle perspective.

Most insects have a complex life cycle with ecologically different larval and adult stages. We present an ontogenetic perspective to analyze and summarize the complex life cycle of Odonata within an evolutionary ecology framework. Morphological, physiological, and behavioral pathways that generate carry-over effects across the aquatic egg and larval stages and the terrestrial adult stage are identified. We also highlight several mechanisms that can decouple life stages including compensatory mechanisms at the larval and adult stages, stressful and stochastic events during metamorphosis, and stressful environmental conditions at the adult stage that may overrule effects of environmental conditions in the preceding stage. We consider the implications of these findings for the evolution, selection, and fitness of odonates; underline the role of the identified numerical and carry-over effects in shaping population and metapopulation dynamics and the community structure across habitat boundaries; and discuss implications for applied conservation issues.

Research trends in mosquito studies in urban areas.

Urbanization is causing a significant impact on biodiversity and human health. The increase in vector-borne diseases in recent decades is linked to changes in the environment due to urbanization. We have reviewed published information on urban mosquitoes from around the globe to examine major study trends in terms of urbanization, and the type of arboviruses they vector. Our review showed a surge in research on urban mosquitoes in the past 15 years, with the majority of studies conducted in the Americas and focusing on Aedes aegypti and Ae. albopictus. However, the findings also highlight a lack of basic monitoring data on mosquito diversity and vector-borne diseases in many countries, which poses a challenge for disease control.

Copulatory courtship, body temperature and infection in Tenebrio molitor.

Ectothermic animals can raise their body temperature under varying circumstances. Two such situations occur during sexual activity (as metabolic rate rises during copulatory movements) and during infection (to control pathogens more effectively). We have investigated these two situations using Tenebrio molitor males. We recorded the copulatory courtship behavior of sick (= infected with Metharizium robertsii fungus) vs healthy males and its link with body temperature. We predicted a positive relation between copulatory courtship (measured as antennal and leg contact behavior) and body temperature, especially in sick males. We found that the intensity of contacts correlated with increased body temperature in sick males. Previous studies in this species indicated that partner females laid fewer eggs after mating with sick males above a certain male body temperature threshold. Thus, our present findings suggest that females may detect male infection via intensity of antennal-mediated courtship, body temperature or their combination. If this is the case, females may assess male cues directly related to health status such as body temperature.