RESUMEN
Smart buildings benefit from IEEE 802.15.4e time slotted channel hopping (TSCH) medium access for creating reliable and power aware wireless sensor and actuator networks (WSANs). As in these networks, sensors are supposed to communicate to each other and with actuators, IPv6 multicast forwarding is seen as a valuable means to reduce traffic. A promising approach to multicast, based on the Routing Protocol for Low Power and Lossy Networks (RPL) is Bidirectional Multicast RPL Forwarding (BMRF). This paper aimed to analyze the performance of BMRF over TSCH. The authors investigated how an adequate TSCH scheduler can help to achieve a requested quality of service (QoS). A theoretical model for the delay and energy consumption of BMRF over TSCH is presented. Next, BMRF's link layer (LL) unicast and LL broadcast forwarding modes were analyzed on restricted and realistic topologies. On topologies with increased interference, BMRF's LL broadcast on top of TSCH causes high energy consumption, mainly because of the amount of energy needed to run the schedule, but it significantly improves packet delivery ratio and delay compared to ContikiMAC under the same conditions. In most cases, the LL unicast was found to outperform the LL broadcast, but the latter can be beneficial to certain applications, especially those sensitive to delays.
RESUMEN
The crenarchaeon Sulfolobus acidocaldarius, growing optimally at temperatures between 75 and 80 °C, thrives in volcanic hot spring habitats that are typified by large temperature gradients, which impose frequent temperature stresses on the cells. Heat shock response is characterized by an upregulation of heat shock proteins, but similar to most (hyper-)thermophilic archaea, S. acidocaldarius seems to be able to bear supra-optimal temperatures with a restricted repertoire of chaperones. Here, we study the physiological consequences of continuous high-temperature stress and rapid heat shock for S. acidocaldarius. Growth experiments and cell viability assays demonstrate that temperatures of 85 °C and higher result in a decreased growth rate and, when the cells are rapidly subjected to a heat shock, a dynamic increase in mRNA levels of all relevant heat shock proteins and a subset of transcription regulators is observed. When exponentially growing cultures are exposed to a heat shock, the survival tipping point is situated around 90 °C, and the rate of heating determines whether cells are able to cope with this stress or whether the defense mechanism immediately fails, leading to extensive cell death. In conclusion, S. acidocaldarius does not seem to be better equipped to handle sudden supra-optimal temperature stress than mesophilic organisms.