Radiative cooling of cationic carbon clusters, CN+, N = 8, 10, 13-16.

The radiative cooling of highly excited carbon cluster cations of sizes N = 8, 10, 13-16 has been studied in an electrostatic storage ring. The cooling rate constants vary with cluster size from a maximum at N = 8 of 2.6 × 104 s-1 and a minimum at N = 13 of 4.4 × 103 s-1. The high rates indicate that photon emission takes place from electronically excited ions, providing a strong stabilizing cooling of the molecules.

Prevalence of Mycoplasma pneumoniae: a cause for community-acquired infection among pediatric populaztion.

BACKGROUND: Atypical pneumonia caused by Mycoplasma pneumoniae is a leading cause of mortality among the pediatric age group. OBJECTIVES: Our study was designed to know the prevalence of M. pneumoniae in children with community-acquired pneumonia and the involvement in the cytoadherence to the respiratory epithelium by M. pneumoniae using electron microscopy and immuno-gold labeling technique. MATERIALS AND METHODS: A total of 152 children of 1 month to 12 years of age of both sexes attending Hebei Provincial People's Hospital, Shijiazhuang, Hebei with diagnosed pneumonia were included in the study. RESULTS: Out of 152 children 84 (55.3%) were males, and 68 (44.7%) were females. The mean age of the patients in the control group (50 patients) was 18.5 ± 3 months with 31 (62%) males and 19 (38%) females. IgM antibodies against M. pneumoniae were positive in 84 (55.3%) males and 68 (44.7%) females. Out of 50 patients 9 (18%) were found to positive for IgM M. pneumoniae antibodies of which four (44.4%) males and 5 (55.5%) females were positive. Our study observed that the gold particles were clustered on the filamentous extension of the tip of the cells. Out of 152 serum samples subjected to particle agglutination assay 138 (90.7%) were positive 1:320 titer, 9 were >1:80 and 3 showed titer was >1:40. CONCLUSION: We suggest that clinicians should consider empirical therapy of broad spectrum antibiotics therapy to cover these atypical pathogens to reduce the severity before obtaining the serological results. From our study, we also suggest electron microscopic and biochemical studies for better diagnosis of these pathogens.

Effects of mycophenolate mofetil on the expression of monocyte chemoattractant protein-1 and fibronectin in high glucose cultured human mesangial cells.

The effects of high glucose on the expression of monocyte chemoattractant protein-1 (MCP-1) and the main component of the extracellular matrix, fibronectin (FN), were explored in human mesangial cells (HMCs), along with the intervention effects of mycophenolate mofetil (MMF) on these indicators. Cultured HMCs were divided into five groups: 1) normal control group (5 mM glucose); 2) high glucose group (30 mM glucose); 3) mannitol osmotic pressure control group (5 mM glucose + 25 mM mannitol); 4) high glucose + MMF-10 group (30 mM glucose + 10 µg/mL MMF); 5) high glucose + MMF-100 group (30 mM glucose + 100 µg/mL MMF). At 24, 48, and 72 h, reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay methods were used to detect the effects of MMF on MCP-1 mRNA and protein and FN expression in HMCs under high glucose conditions. MCP-1 mRNA and protein expressions and FN secretion significantly increased in HMCs of the high glucose group compared with the normal control group (P < 0.01), with the highest expression observed at 48 h. MMF could reduce the MCP-1 mRNA and protein and FN expression levels (P < 0.01), and the inhibition occurred in a dose- and time-dependent manner (P < 0.05). In conclusion, MMF could inhibit MCP-1 expression and the secretion of FN, indicating that it may delay the progression of glomerulosclerosis and interstitial fibrosis in diabetic nephropathy to ultimately achieve protective effects on the kidney.

[Incidence and prognosis of olfactory and gustatory dysfunctions related to infection of SARS-CoV-2 Omicron strain: a national multi-center survey of 35 566 population].

Objective: This cross-sectional investigation aimed to determine the incidence, clinical characteristics, prognosis, and related risk factors of olfactory and gustatory dysfunctions related to infection with the SARS-CoV-2 Omicron strain in mainland China. Methods: Data of patients with SARS-CoV-2 from December 28, 2022, to February 21, 2023, were collected through online and offline questionnaires from 45 tertiary hospitals and one center for disease control and prevention in mainland China. The questionnaire included demographic information, previous health history, smoking and alcohol drinking, SARS-CoV-2 vaccination, olfactory and gustatory function before and after infection, other symptoms after infection, as well as the duration and improvement of olfactory and gustatory dysfunction. The self-reported olfactory and gustatory functions of patients were evaluated using the Olfactory VAS scale and Gustatory VAS scale. Results: A total of 35 566 valid questionnaires were obtained, revealing a high incidence of olfactory and taste dysfunctions related to infection with the SARS-CoV-2 Omicron strain (67.75%). Females(χ2=367.013, P<0.001) and young people(χ2=120.210, P<0.001) were more likely to develop these dysfunctions. Gender(OR=1.564, 95%CI: 1.487-1.645), SARS-CoV-2 vaccination status (OR=1.334, 95%CI: 1.164-1.530), oral health status (OR=0.881, 95%CI: 0.839-0.926), smoking history (OR=1.152, 95%CI=1.080-1.229), and drinking history (OR=0.854, 95%CI: 0.785-0.928) were correlated with the occurrence of olfactory and taste dysfunctions related to SARS-CoV-2(above P<0.001). 44.62% (4 391/9 840) of the patients who had not recovered their sense of smell and taste also suffered from nasal congestion, runny nose, and 32.62% (3 210/9 840) suffered from dry mouth and sore throat. The improvement of olfactory and taste functions was correlated with the persistence of accompanying symptoms(χ2=10.873, P=0.001). The average score of olfactory and taste VAS scale was 8.41 and 8.51 respectively before SARS-CoV-2 infection, but decreased to3.69 and 4.29 respectively after SARS-CoV-2 infection, and recovered to 5.83and 6.55 respectively at the time of the survey. The median duration of olfactory and gustatory dysfunctions was 15 days and 12 days, respectively, with 0.5% (121/24 096) of patients experiencing these dysfunctions for more than 28 days. The overall self-reported improvement rate of smell and taste dysfunctions was 59.16% (14 256/24 096). Gender(OR=0.893, 95%CI: 0.839-0.951), SARS-CoV-2 vaccination status (OR=1.334, 95%CI: 1.164-1.530), history of head and facial trauma(OR=1.180, 95%CI: 1.036-1.344, P=0.013), nose (OR=1.104, 95%CI: 1.042-1.171, P=0.001) and oral (OR=1.162, 95%CI: 1.096-1.233) health status, smoking history(OR=0.765, 95%CI: 0.709-0.825), and the persistence of accompanying symptoms (OR=0.359, 95%CI: 0.332-0.388) were correlated with the recovery of olfactory and taste dysfunctions related to SARS-CoV-2 (above P<0.001 except for the indicated values). Conclusion: The incidence of olfactory and taste dysfunctions related to infection with the SARS-CoV-2 Omicron strain is high in mainland China, with females and young people more likely to develop these dysfunctions. Active and effective intervention measures may be required for cases that persist for a long time. The recovery of olfactory and taste functions is influenced by several factors, including gender, SARS-CoV-2 vaccination status, history of head and facial trauma, nasal and oral health status, smoking history, and persistence of accompanying symptoms.

Intensity modulated radiation therapy (IMRT) surface dose measurements using a PTW advanced Markus chamber.

A new PTW advanced Markus ionization chamber has been implemented in IMRT fields, to measure surface dose at ICRU and ICRP reference depth of 0.07 mm [ICRU Report 39, Determination of dose equivalents resulting from external radiation sources, 1985; ICRP Publication 60, 1990 recommendations of the International Commission on Radiological Protection, 1991]. This chamber has a small radius with a revised guard ring design, therefore less prone to surface over-response effects. The over response correction for advanced Markus chamber is 3.3%, which is significantly smaller than 10.1% which was the original Markus chamber over response. After over response correction, the surface dose can be accurately measured by either data extrapolation or by adding one layer of plastic sheet protector to the top of Markus chamber. The surface dose measurements for small fields, e.g 3 x 3 cm, the polarity effect of advanced Markus chamber is 12%, which is significantly higher than the 5% polarity effect of the original Markus. For a 12 x 12 cm field size, surface dose (at 0.07 mm) measured by advanced Markus chamber is 19.8% for open field and 19.2% for an unmodulated step-and-shoot IMRT field. The variation in surface dose due to intensity modulated IMRT fields has also been investigated. For an intensity modulated, step-wedge IMRT field, surface dose varies from 15.7 + or - 1% for the highest intensity segment to 26.9 + or - 1% for the lowest intensity segment. The results of chamber measurements have been compared against EBT type GAFCHROMIC film results.

[Effect of sublingual dust mite drops on single or multiple allergens allergic rhinitis in children].

Objective:Evaluation of the efficacy of standardized dust mite drops in sublingual immunotherapy(SLIT) for allergic rhinitis in children.Method:A retrospective analysis of 174 children who received SLIT with standardized dermatophagoides farinae drops for 2 years.These patients had been divided into two groups:monoasensitized group(n=61) and polysensitized group(n=113).The total medication score(TMS),total nasal symptoms score(TNSS) and inflammatory factors were evaluated before and after SLIT treatment.Result:①After SLIT treatment for 2.0 year,the TNSS in the monosensitized group is(11.27±1.46) and(3.48±1.50),polyasensitized group is (11.54±1.50) and (3.59±1.56),there are significant difference of TNSS between two groups(P<0.01).But the improvements of the TNSS between the two groups have no significant difference(P>0.05),the monosensitized group is(7.68±3.23); polysensitized group is (8.14±2.56). ②Two groups of children with TMS before and after treatment were obviously improved, monosensitized group is (1.67±0.43) and (0.52±0.40),polysensitized group is(1.64±0.44) and (0.55±0.41). There are significant difference of TMS between two groups(P<0.01).But the improvements of the TMS between the two groups have no obvious difference(P>0.05),the monsensitized group is(1.16±0.61); polysensitized group is(1.28±0.55).③Specific IgG4 serum is increased after treatment(P<0.01).④After immunotherapy,the expression of IL4 and IL-17α is downregulated, IL-2 and TGF-ß1 is up-regulated.Conclusion:①Dust mites under the SLIT,can significantly improve the monosensitized and polyasensitized allergic children nasal symptoms,reduce the drug use, and two groups have the equivalent effect.②Dust mite drops SLIT,can be used to the monosensitized and polyasensitized allergic children.③The rise of dust mites specific IgG4 can be used as immunotherapy effective predictors.④After immunotherapy, Thl/Th2 /Thl7 and Treg can be rebalanced.

Cold-Specific Induction of a Dehydrin Gene Family Member in Barley.

An interval on barley (Hordeum vulgare L.) chromosome 7 accounting for significant quantitative trait locus effects for winter hardiness were detected in a winter (Dicktoo) x spring (Morex) barley population (P.M. Hayes, T. Blake, T.H.H. Chen, S. Tragoonrung, F. Chen, A. Pan, and B. Liu [1993] Genome 36: 66-71). Two members of the barley dehydrin gene family, Dhn1 and Dhn2, were located within the region defining the winter hardiness quantitative trait locus effect (A. Pan, P.M. Hayes, F. Chen, T. Blake, T.H.H. Chen, T.T.S. Wright, I. Karsai, Z. Bedo [1994] Theor Appl Genet 89: 900-910). To investigate the possible role of Dhn1 and Dhn2 in winter hardiness, we examined the expression pattern of six barley dehydrin gene family members in shoot tissue in response to cold temperature. Incubation of 3-week-old barley plants at 2[deg]C resulted in a rapid induction of a single 86-kD polypeptide that was recognized by an antiserum against a peptide conserved in the dehydrin gene family. Northern blot analysis confirmed the induction of an mRNA corresponding to Dhn5. The expression patterns of cold-induced dehydrins in shoot tissue for Dicktoo and Morex were identical under the conditions studied, in spite of the known phenotypic differences in their winter hardiness. These results, together with the allelic structure of selected high- and low-survival lines, suggest that the Dicktoo alleles at the Dhn1 and Dhn2 may not be the primary determinants of winter hardiness in barley.

Physiological integration enhanced the tolerance of Cynodon dactylon to flooding.

Many flooding-tolerant species are clonal plants; however, the effects of physiological integration on plant responses to flooding have received limited attention. We hypothesise that flooding can trigger changes in metabolism of carbohydrates and ROS (reactive oxygen species) in clonal plants, and that physiological integration can ameliorate the adverse effects of stress, subsequently restoring the growth of flooded ramets. In the present study, we conducted a factorial experiment combining flooding to apical ramets and stolon severing (preventing physiological integration) between apical and basal ramets of Cynodon dactylon, which is a stoloniferous perennial grass with considerable flooding tolerance. Flooding-induced responses including decreased root biomass, accumulation of soluble sugar and starch, as well as increased activity of superoxide dismutase (SOD) and ascorbate peroxidase (APX) in apical ramets. Physiological integration relieved growth inhibition, carbohydrate accumulation and induction of antioxidant enzyme activity in stressed ramets, as expected, without any observable cost in unstressed ramets. We speculate that relief of flooding stress in clonal plants may rely on oxidising power and electron acceptors transferred between ramets through physiological integration.

RAPD markers associated with salt tolerance in an interspecific cross of tomato (Lycopersicon esculentum×L. pennellii).

This study was conducted to identify randomly amplified polymorphic DNA (RAPD) markers associated with quantitative trait loci (QTLs) conferring salt tolerance during germination in tomato. Germination response of an F2 population (2000 individuals) of a cross between UCT5 (Lycopersicon esculentum, salt-sensitive) and LA716 (L. pennellii, salt-tolerant) was evaluated at a salt-stress level of 175 mM NaCl+17.5 mM CaCl2 (water potential ca. -9.5 bars). Germination was scored visually as radicle protrusion at 6-h intervals for 30 consecutive days. Individuals at both extremes of the response distribution (i.e., salt-tolerants and salt-sensitives) were selected. The selected individuals were genotyped for 53 RAPD markers and allele frequencies at each marker locus were determined. The linkage association among the markers was determined using a "MAPMAKER" program. Trait-based marker analysis (TBA) identified 13 RAPD markers at eight genomic regions that were associated with QTLs affecting salt tolerance during germination in tomato. Of these genomic regions, five included favorable QTL alleles from LA716, and three included favorable alleles from UCT5. The approximate effects of individual QTLs ranged from 0.46 to 0.82 phenotypic standard deviation. The results support our previous suggestion that salt tolerance during germination in tomato is polygenically controlled. The identification of favorable QTLs in both parents suggests the likelihood of recovering transgressive segregants in progeny derived from these genotypes. Results from this study are discussed in relation to using marker-assisted selection in breeding for salt tolerance.

Effects of active immunization against GnRH on serum LH, inhibin A, sexual development and growth rate in Chinese female pigs.

Surgical castration of young female pigs is common practice in Chinese pig farming today. The purpose of the present study is to investigate anti-GnRH immunization as a practical alternative to surgical castration for female pigs. Thirty-six Chinese female crossbred pigs (Chinese Yanan x Yorkshire) were selected from 12 litters, three pigs from each litter, at the age of 10-13 weeks. One pig from each litter was immunized with 62.5 microg D-Lys6-GnRH-tandem-dimer peptide conjugated to ovalbumin in Specol adjuvant at Week 0 (0 week post-vaccination, wpv), and a booster vaccination was given 8 weeks later (8 wpv). Its intact and castrate littermates (surgically castrated at the time of weaning, i.e. at 6 weeks of age) were administered the vehicle and served as controls. Antibody titers, serum LH and inhibin A were determined at the day of first vaccination, every 4 weeks thereafter and at the day of slaughter (18 wpv). At slaughter, ovaries were inspected for the presence of follicles and corpora lutea, and ovarian and uterine weights were recorded. Ten of twelve immunized pigs responded well to the immunization (immunocastrated animals), while the remaining two pigs responded poorly (nonresponders). Antibody titres in immunocastrated animals steadily increased after immunization, became maximal at 12 wpv and remained high until slaughter. Serum LH levels were reduced (P < 0.05) in immunocastrated pigs as compared to intact controls and surgical castrates. Serum inhibin A levels decreased after vaccination, and equaled surgical castrate levels from 8 wpv until the end of the experiment. Ovarian and uterine weights (1.3 +/- 0.2 and 43.9 +/- 11.4 g, respectively; mean +/- S.E.M.) were significantly lower (P < 0.05) in immunocastrates than in intact controls (9.4 +/- 1.1 and 390.9 +/- 67.2 g, respectively). Antibody titers were significantly lower (P < 0.05) in nonresponders than in immunocastrated pigs from 12 wpv to slaughter. Ovarian and uterine weights were similar in nonresponders and in intact controls. Macroscopically, no follicular structures were found in ovaries of immunocastrated pigs, while large follicles or corpora lutea were observed in the ovaries of both nonresponders and intact controls. Although not significant, immunocastrates had a numerically higher average daily gain than surgical castrates and intact controls (0.74 +/- 0.04 versus 0.66 +/- 0.04 versus 0.66 +/- 0.03 kg per day, respectively; mean +/- S.E.M., P = 0.09). Results obtained in the present study demonstrate that anti-GnRH immunization can be an attractive alternative to surgical castration for Chinese crossbred female pigs. Our results also question the beneficial effect of surgical castration on growth as compared to intact controls.

[Study on the transperitoneal sterilization of the fallopian tube with silicon rubber plug and its reversibility].

PIP: A blocking device made of silicon rubber was adopted for tubal blocking by transperitoneal manipulation on 30 domestic rabbits with an observation period of 3-24 months. 100% success with sterilization was obtained. Removal of the plug transperitoneally was carried out on 16 rabbits with successful sterilization and 15 of the 16 were pregnant within 2 months. Successful rates of pregnancy amounted to 93.75%. Histological study revealed that the continuity of the tubal lumina was maintained by the use of this procedure with very little damage to the tissues, and that whatever damaged tissue there was, it would be completely recovered after removal of the plug. Thus, the reversibility of the procedure is clear. This blocking procedure with silastic rubber plugs was utilized among 232 women in clinical trials beginning in June, 1984. The longest observation period was 42 months, the shortest 12. 1 patient did not have a successful procedure, thus bringing the sterilization rate down to 99.57%. In May, 1987, plugs were removed from 8 women who had been successfully sterilized for 1 year; of these 8 women, 7 have achieved an intrauterine pregnancy within 1 year. (author's modified)^ieng

A comparison of Hordeum bulbosum-mediated haploid production efficiency in barley using in vitro floret and tiller culture.

A high efficiency of Hordeum bulbosum-mediated haploid production in barley has been achieved using a floret culture technique in which florets pollinated with Hordeum bulbosum are cultured on modified N6 media containing 0.5 mg/l kinetin and 1.2 mg/l2,4-D. Cultures were maintained at 25 °C with a 16 h photoperiod for 9 days before embryo rescue. In a comparison of haploid production efficiency using five F1 hybrids from winter x winter and winter x spring barley crosses, 41.6 haploid plants/100 florets pollinated were produced using floret culture. Using detached tiller culture, 13.5 haploid plants/100 florets pollinated were produced. Higher efficiencies achieved with floret culture are attributed to the formation of larger, differentiated embryos. Such embryos lead to higher frequencies of plant regeneration. The F1 from a winter x winter cross was inferior in haploid production compared to F1s from winter x spring crosses. No genotype x technique interaction was observed.

[Vasomotor rhinitis following endoscopic sinus surgery: 5 cases report].

OBJECTIVE: To investigate the possibility of endoscopic sinus surgery causing vasomotor rhinitis. METHOD: A retrospective study of 300 patients who received endoscopic sinus surgery. 5 cases out of 300 suffered from vasomotor rhinitis 1-3 weeks following endoscopic sinus surgery. Radio frequency cautery of agger nasi mucous was applied to all 5 patients. RESULT: After 4-6 months of follow-up, 4 cases out of 5 with vasomotor rhinitis were effective and 1 improved. CONCLUSION: Endoscopic sinus surgery may be causative factor for vasomotor rhinitis.

[The expression of transforming growth factor alpha and its receptor in nasal polyps].

OBJECTIVE: To assess the possible role of expression of TGF alpha and EGFR in nasal polyps and its relationship with PCNA labeling index. METHOD: Specimens from 20 patients of nasal polyps were studied with immunohistochemical technique. RESULT: The expression of TGF alpha, EGFR and PCNA were increased in the epithelium, gland cells and inflammatory cells of nasal polyps. There was a close correlation between the intensities of TGF alpha, EGFR and PCNA. CONCLUSION: TGF alpha may play a key role in epithelial cell proliferation in nasal polyps.