[New clone of ST-187 Acinetobacter baumannii responsible for an outbreak in an intensive care unit]. / Nuevo clon de Acinetobacter baumannii ST187 responsable de un brote en una unidad de cuidados intensivos.

INTRODUCTION: An ICU-outbreak caused by a novel Acinetobacter baumannii clone is described. METHODS: An active search of carriers and environmental reservoirs was carried out. Carbapenemases genes were studied using multiplex-PCR and genotypic analysis by rep-PCR, PFGE and MLST. RESULTS: A total 26 infected patients and 10 carriers were identified. A.baumannii was recovered from infusion pumps, walls, floor and washbasins. Phenotypic/genotypic analysis showed clonal expansion of a unique clone ST-187 producer of type OXA-24 and OXA-51 carbapenemases. DISCUSSION: This is the first outbreak caused by ST-187 (ECI/GCI) multiresistant A.baumannii.

Methicillin-resistant Staphylococcus aureus in swine housed indoors in Galicia, Spain.

INTRODUCTION: Livestock are known reservoirs of methicillin-resistant Staphylococcus aureus (MRSA) and this constitutes an important public health issue. The prevalence of nasal MRSA carriers in swine housed indoors in Galicia, Spain, was studied. METHODS: 197 samples from swine aged three, eight, 12, 16 and 24 weeks, and from adult pigs, were obtained from four farms. The cleaning procedures implemented to clean the barns and antimicrobial consumption were analyzed. Antimicrobial susceptibility and antimicrobial resistance genes were studied. PFGE, spa typing and MLST were used to classify the isolates. SCCmec, agr and pvl were analyzed. RESULTS: MRSA prevalence was 12.7%. Swine younger than 16 weeks had a higher colonization rate; 22.9% vs 3.5% (OR, 8.16; 95% CI, 2.47-29.79; p<0.01). The only farm found to be MRSA-free used disinfectants as part of its cleaning procedure. All MRSA were tetracycline-resistant (identifying the tetK and tetM genes), 80% were resistant to erythromycin and clindamycin and 16% were only clindamycin-resistant. The ermC and vgaA genes were identified in these two phenotypes. A single genotype (PFGE type A) and ST398 - spa t011 (84%) and t1451 (16%) were identified. SCCmec type V and agrI were identified in all isolates, and all were pvl-negative. CONCLUSION: A correlation between swine age and MRSA colonization was observed. Appropriate cleaning procedures could have an impact on MRSA colonization in farming. Resistance to antibiotics used in human health was identified. Clinicians should be aware if their patients have come into contact with farm animals.

HLA-DQ B1*0201 and A1*0102 Alleles Are Not Responsible for Antituberculosis Drug-Induced Hepatotoxicity Risk in Spanish Population.

AIMS: To evaluate the role of human leukocyte antigen (HLA) class II DQB1*0201 and DQA1*0102 in the risk of antituberculosis drug (ATD)-induced hepatotoxicity (ATDH) in a cohort of tuberculosis patients of Caucasian origin from Spain. METHODS: Matched case-control study including active tuberculosis (TB) patients from Spain (Caucasian) treated with first-line ATD (Isoniazid, Rifampin, and Pyrazinamide). Presence or absence of HLA class II DQB1*0201 and DQA1*0102 alleles were compared between cases and controls. RESULTS: We included 110 TB patients, 55 ATDH cases, and 55 sex-matched controls. The analysis of the presence of HLA-DQB1*0201 and HLA-DQA*0102 did not show significative differences between both groups [presence of HLA-DQB1*0201 53.6% of the cases vs. 45.4% of the controls, OR: 1.63 95% CI (0.62-4.52) p = 0.38; presence of HLA-DQA*0102 7.5% of cases vs. 20% of controls, OR: 0.36 95% CI (0.08-1.23) p = 0.12]. After multivariate logistic regression analysis including in the model, other potential risk factors of hepatotoxicity HLA class II DQB1*0201 and DQA1*0102 alleles were not found significantly associated with the risk of development ATDH. We could not demonstrate an association between HLA-DQA1*0102 and HLA-DQB1*0201 with the risk of ATDH in this Caucasian population of Spanish origin.

Methicillin-resistant Staphylococcus aureus in swine housed indoors in Galicia, Spain / Staphylococcus aureus resistente a la meticilina en cerdos estabulados en Galicia, España

INTRODUCTION: Livestock are known reservoirs of methicillin-resistant Staphylococcus aureus (MRSA) and this constitutes an important public health issue. The prevalence of nasal MRSA carriers in swine housed indoors in Galicia, Spain, was studied. METHODS: 197 samples from swine aged three, eight, 12, 16 and 24 weeks, and from adult pigs, were obtained from four farms. The cleaning procedures implemented to clean the barns and antimicrobial consumption were analyzed. Antimicrobial susceptibility and antimicrobial resistance genes were studied. PFGE, spa typing and MLST were used to classify the isolates. SCCmec, agr and pvl were analyzed. RESULTS: MRSA prevalence was 12.7%. Swine younger than 16 weeks had a higher colonization rate; 22.9% vs 3.5% (OR, 8.16; 95% CI, 2.47-29.79; p < 0.01). The only farm found to be MRSA-free used disinfectants as part of its cleaning procedure. All MRSA were tetracycline-resistant (identifying the tetK and tetM genes), 80% were resistant to erythromycin and clindamycin and 16% were only clindamycin-resistant. The ermC and vgaA genes were identified in these two phenotypes. A single genotype (PFGE type A) and ST398 - spa t011 (84%) and t1451 (16%) were identified. SCCmec type V and agrI were identified in all isolates, and all were pvl-negative. CONCLUSION: A correlation between swine age and MRSA colonization was observed. Appropriate cleaning procedures could have an impact on MRSA colonization in farming. Resistance to antibiotics used in human health was identified. Clinicians should be aware if their patients have come into contact with farm animals

INTRODUCCIÓN: Los animales de granja son reservorios de Staphylococcus aureus resistente a la meticilina (SARM), y constituyen un problema de salud pública. Se estudia la prevalencia de portadores nasales de SARM en cerdos estabulados en Galicia, España. MÉTODOS: En 4 explotaciones se obtuvieron 197 muestras de cerdos con edades en semanas de 3, 8, 12, 16, 24 y adultos. Se analizaron los métodos empleados para limpiar los establos y el consumo de antimicrobianos. Se estudió la resistencia a antimicrobianos, y los genes involucrados en esta. Los aislamientos fueron clasificados mediante PFGE, spa y MLST. Se analizaron SCCmec, agr y pvl. RESULTADOS: La prevalencia de SARM fue del 12,7%. Los cerdos de <16 semanas presentaron las frecuencias de colonización más elevadas 22,9 vs. 3,5% (OR: 8,16; IC 95%: 2,47-29,79; p < 0,01). En la única explotación libre de SARM se empleaban desinfectantes en la limpieza. Todos los SARM fueron resistentes a tetraciclina identificándose los genes tetK y tetM, el 80% fueron resistentes a eritromicina y clindamicina, y el 16% fueron únicamente resistentes a clindamicina. Se identificaron los genes ermC y vgaA en estos 2 fenotipos. Se identificó un único genotipo (PFGE-A) y ST398, siendo spa t011 (84%) y t1451 (16%). En todos los aislamientos se identificó SCCmec V y agrI, siendo estos pvl negativos. CONCLUSIONES: Se observó la asociación entre edad y colonización SARM. La limpieza adecuada podría modificar la colonización por SARM. Se detectaron resistencias a antibióticos empleados en humanos. Los médicos deberían conocer si los pacientes tienen contacto con animales de granja

Nuevo clon de Acinetobacter baumannii ST187 responsable de un brote en una unidad de cuidados intensivos / New clone of ST-187 Acinetobacter baumannii responsible for an outbreak in an intensive care unit

INTRODUCCIÓN: Se describe un brote causado por un nuevo clon de Acinetobacter baumannii multirresistente (ABMR). MÉTODOS: Se realizó una búsqueda activa de portadores y de reservorios ambientales, detección de carbapenemasas por PCR-multiplex y análisis genotípico por rep-PCR, PFGE y MLST. RESULTADOS: Se identificaron 26 pacientes infectados y 10 colonizados. A. baumannii se recuperó de bombas de infusión, paredes, suelo y lavamanos. El estudio feno/genotípico mostró la expansión clonal de un único clon ST-187 productor de carbapenemasas tipo OXA-24 y OXA-51. DISCUSIÓN: Se trata del primer brote causado por A. baumannii multirresistente ST-187 (EC I/GC I)

INTRODUCTION: An ICU-outbreak caused by a novel Acinetobacter baumannii clone is described. METHODS: An active search of carriers and environmental reservoirs was carried out. Carbapenemases genes were studied using multiplex-PCR and genotypic analysis by rep-PCR, PFGE and MLST. RESULTS: A total 26 infected patients and 10 carriers were identified. A. baumannii was recovered from infusion pumps, walls, floor and washbasins. Phenotypic/genotypic analysis showed clonal expansion of a unique clone ST-187 producer of type OXA-24 and OXA-51 carbapenemases. DISCUSSION: This is the first outbreak caused by ST-187 (ECI/GCI) multiresistant A. baumannii