RESUMEN
Glioblastoma is the most lethal tumor of the central nervous system, presenting a very poor prognostic, with a survival around 16 months. The interaction of mesenchymal stem cells and tumor cells has been studied, showing a bias in their role favoring or going against aggressiveness. Natural products such as flavonoids have showed their anticancer properties and the synergic potential with the activation of microenvironment cells to inhibit tumor progression. Agathisflavone is a flavonoid studied in neurodegenerative diseases and cancer. The present study investigated the effect of flavonoid in the viability of heterogeneous glioblastoma (GBM) cells considering a coculture or conditioned medium of mesenchymal stem cells (MSCs) effect, as well as the dose-dependent effect of this flavonoid in tumor migration and differentiation via STAT3. Agathisflavone (3-10 µM) induced dose-dependent toxicity to GL-15 and U373 human GBM cells, since 24 h after treatments. It was not toxic to human MSC but modified the pattern of interaction with GBM cells. Agathisflavone also inhibited migration and increased differentiation of human GBM cells, associated with the reduction on the expression of STAT3. These results demonstrate that the flavonoid agathisflavone had a direct anti-glioma effect. However, could be observed its effect in MSCs response that may have an impact in controlling GBM growth and aggressiveness, an important factor to consider for new therapies.
Asunto(s)
Antineoplásicos/farmacología , Biflavonoides/farmacología , Neoplasias Encefálicas/tratamiento farmacológico , Glioblastoma/tratamiento farmacológico , Células Madre Mesenquimatosas/metabolismo , Neoplasias Encefálicas/patología , Diferenciación Celular/efectos de los fármacos , Línea Celular Tumoral , Movimiento Celular/efectos de los fármacos , Proliferación Celular/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Técnicas de Cocultivo , Medios de Cultivo Condicionados/farmacología , Glioblastoma/patología , Humanos , Factor de Transcripción STAT3/metabolismoRESUMEN
This study describes the isolation and identification of apigenin-7-O-ghicopyranoside, a flavonoid isolated from the flowers of Bellis perennis L., Asteraceae, an species with a broad spectrum of biological activities. The in vitro antioxidant activity and the inhibition of the enzyme acetylcholinesterase were evaluated. The flavonoid showed strong in vitro antioxidant potential, because of the capacity of removal of hydroxyl radicals and nitric oxide, and also prevented the formation of thiobarbituric acid-reactive substances. These parameters were inhibited at the highest concentration of ApG at rates of 77.7%, 72% and 73.4%, respectively, in addition to inhibiting acetylcholinesterase, suggesting potential use in the treatment of neurodegenerative diseases.
Asunto(s)
Antioxidantes/farmacología , Asteraceae/química , Inhibidores de la Colinesterasa/farmacología , Flores/química , Extractos Vegetales/farmacología , Acetilcolinesterasa/efectos de los fármacos , Asteraceae/clasificación , Peroxidación de Lípido/efectos de los fármacos , Extractos Vegetales/aislamiento & purificaciónRESUMEN
Plectranthus spp (Lamiaceae) are plants of economic importance because they are sources of aromatic essential oils and are also cultivated and several species of this genus are used as folk medicines. This paper describes the effects of different concentrations of the 2,4-dichlorophenoxyacetic acid (2,4-D) and 1-naphthaleneacetic acid (NAA) on the induction of callus from nodal segments of Plectranthus ornatus Codd and in the production of volatile organic compounds (monoterpenes and sesquiterpenes). The 20 and 40 day calli were subjected to solid phase micro extraction (HS-SPME) and submitted to GCMS analysis. Variations in VOCs between the samples were observed and, a direct relationship was observed between of the major constituent detected (α-terpinyl acetate) and the monoterpenes α-thujene, α-pinene, ß-pinene, camphene, sabinene and α-limonene that were present in the volatile fractions. Besides α-terpinyl acetate, isobornyl acetate and α-limonene were also major constituents. Variations were observed in VOCs in the analyzed periods. The best cultivation media for the production of VOCs was found to be MS0 (control). Moderate success was achieved by treatment with 2.68 µM and 5:37 µM NAA (Group 2). With 2,4-D (9.0 µM), only the presence of α-terpinyl acetate and isocumene were detected and, with 2.26 µM of 2,4-D was produced mainly α-terpinyl acetate, α-thujene and ß-caryophyllene (16.2%). The VOC profiles present in P. ornatus were interpreted using PCA and HCA. The results permitted us to determine the best cultivation media for VOC production and, the PCA and HCA analysis allowed us to recognize four groups among the different treatments from the compounds identified in this set of treatments.
Asunto(s)
Monoterpenos/aislamiento & purificación , Componentes Aéreos de las Plantas/química , Plectranthus/química , Sesquiterpenos/aislamiento & purificación , Compuestos Orgánicos Volátiles/aislamiento & purificación , Cromatografía de Gases y Espectrometría de Masas , Monoterpenos/química , Monoterpenos/metabolismo , Aceites Volátiles/química , Aceites Volátiles/aislamiento & purificación , Componentes Aéreos de las Plantas/crecimiento & desarrollo , Componentes Aéreos de las Plantas/metabolismo , Extractos Vegetales/química , Extractos Vegetales/aislamiento & purificación , Aceites de Plantas/química , Aceites de Plantas/aislamiento & purificación , Plectranthus/crecimiento & desarrollo , Plectranthus/metabolismo , Sesquiterpenos/química , Sesquiterpenos/metabolismo , Microextracción en Fase Sólida , Compuestos Orgánicos Volátiles/química , Compuestos Orgánicos Volátiles/metabolismoRESUMEN
CONTEXT: Cratylia mollis Martius ex Benth. and Cenostigma macrophyllum Tul. (Leguminosae) are both endemic Brazilian plants and they are used by the natives as medicinal plants, and the leaves of C. mollis are also employed as forage for cattle during the dry season of region. OBJECTIVE: Isolation of the compounds responsible for the acetylcholinesterase (AChE) inhibition from the CHCl3 active extract. MATERIALS AND METHODS: Two peptidic compounds were isolated by chromatographic techniques from the CHCl3 extract of the leaves of C. mollis and C. macrophyllum. They were identified by spectrometric data analysis (MS and NMR) and they were subjected to AChE inhibition employing Ellman's test. RESULTS: The peptides were identified as N-benzoylphenylalaninoyl-phenlyalaninolacetate (aurentiamide acetate) (1) and N-benzoylphenylalaninyl-N-benzoylphenylalaninate (2). Both peptides 1 and 2 exhibit AChE inhibition, with IC50 values equal to 111.34 µM and 137.6 µM, respectively. DISCUSSION AND CONCLUSION: Compound 1 (aurentiamide acetate) has rarely been isolated from the Leguminosae family, and N-benzoylphenylalaninyl-N-benzoylphenylalaninate (2) is a compound that has never previously been isolated from this family. Compound 1 is shown to be a potent inhibitor of AChE, with IC50 values similar to the physostigmine control (141.51 µM).
Asunto(s)
Acetilcolinesterasa/efectos de los fármacos , Inhibidores de la Colinesterasa/farmacología , Fabaceae/química , Extractos Vegetales/farmacología , Acetatos/administración & dosificación , Acetatos/aislamiento & purificación , Acetatos/farmacología , Acetilcolinesterasa/metabolismo , Compuestos de Bencilo/administración & dosificación , Compuestos de Bencilo/aislamiento & purificación , Compuestos de Bencilo/farmacología , Inhibidores de la Colinesterasa/aislamiento & purificación , Dipéptidos/administración & dosificación , Dipéptidos/aislamiento & purificación , Dipéptidos/farmacología , Concentración 50 Inhibidora , Espectroscopía de Resonancia Magnética , Espectrometría de Masas , Fisostigmina/farmacología , Extractos Vegetales/administración & dosificación , Extractos Vegetales/química , Hojas de la PlantaRESUMEN
Betulinic, ursolic and oleanolic acids isolated from the aerial parts of Eriope blanchetii (Lamiaceae) were subjected to different esterification reactions, yielding 12 C-3 position ester derivatives. All compounds were identified using spectroscopic techniques, such as IR, 1H-NMR and MS. The derivatives were further investigated for their antioxidant level, Artemia salina lethality and antimicrobial activity.
Asunto(s)
Ácido Oleanólico/farmacología , Triterpenos/farmacología , Animales , Artemia/efectos de los fármacos , Ésteres/química , Depuradores de Radicales Libres/química , Depuradores de Radicales Libres/farmacología , Lamiaceae/química , Resonancia Magnética Nuclear Biomolecular , Ácido Oleanólico/química , Triterpenos Pentacíclicos , Extractos Vegetales/química , Triterpenos/química , Ácido Betulínico , Ácido UrsólicoRESUMEN
Bergenin (1) is a C-glucoside of 4-O-methylgallic acid with known antiarthritic activity attributed to modulation of cytokine production. The present study was undertaken to evaluate whether 1 has antinociceptive properties in models of inflammatory pain and to investigate its possible mechanisms of action. Pretreatment with 1 (12.5-100 mg/kg, ip) produced a dose-related inhibition of acetic acid-induced writhing in mice. Furthermore, treatment with 1 (50 and 100 mg/kg) inhibited both the early and late phases in a formalin test. In addition, 1 (50 and 100 mg/kg, ip) inhibited mechanical hyperalgesia, edema, and paw production of hyperalgesic cytokines (TNF-α and IL-1ß) induced by complete Freund's adjuvant. However, the local production of IL-10, an anti-inflammatory cytokine, was not altered by 1 (100 mg/kg, ip). Treatment with 1 produced a similar profile of antinociception in wild-type and IL-10-deficient mice. Mice treated with 1 did not show any motor performance alterations or apparent systemic toxicity. The results presented herein demonstrate that bergenin has consistent antinociceptive and anti-inflammatory properties, acting by the inhibition of IL-1ß and TNF-α production, and suggest its potential for the control of inflammatory pain.
Asunto(s)
Analgésicos/farmacología , Antiinflamatorios no Esteroideos/farmacología , Benzopiranos/farmacología , Dolor/tratamiento farmacológico , Analgésicos/química , Animales , Antiinflamatorios no Esteroideos/química , Benzopiranos/química , Relación Dosis-Respuesta a Droga , Edema/inducido químicamente , Edema/tratamiento farmacológico , Ácido Gálico/análogos & derivados , Ácido Gálico/química , Interleucina-10/antagonistas & inhibidores , Interleucina-10/biosíntesis , Interleucina-10/genética , Masculino , Ratones , Dolor/inducido químicamente , Dimensión del Dolor , Factor de Necrosis Tumoral alfa/antagonistas & inhibidores , Factor de Necrosis Tumoral alfa/biosíntesisRESUMEN
Erythroxylaceae is a family composed of four genera, with Erythroxylum being the only one represented in the Neotropical region. Chemical studies indicate the presence of alkaloids, terpenes, flavonoids, and phenolic compounds as main compounds. The incorporation of cytotoxic activity assays of natural products using cell cultures assists in the selection of potential chemotherapeutic agents. In this work, we describe a revision of the cytotoxicity evaluation studies performed with extracts or pure substances obtained from Erythroxylum species through an integrative review. We found studies that evaluated the cytotoxic activity of 21 species of Erythroxylum against 45 different cell lines. The analysis of the chemical composition of these species shows that the metabolites present in each species influence their cytotoxic potential, especially the presence of disubstituted tropane alkaloid species with the highest cytotoxic potential. MTT and Sulforrodamine B assays were the main in vitro tests used for the evaluation of the cytotoxic activities. From the total species, less than 10% of the Erythroxylum species have already been evaluated for cytotoxic activity. Four of them showed high cytotoxic activity according to the criteria of the NCI plant screening program. Thus, this genus represents a potential source of natural products with antitumor activity.
Asunto(s)
Erythroxylaceae/química , Tropanos/farmacología , Antineoplásicos/farmacología , Productos Biológicos/farmacología , Extractos Vegetales/farmacologíaRESUMEN
Ryanodane diterpenes, named 14-O-methyl-ryanodanol and ryanodanol, were isolated from ripe fruit of Erythroxylum passerinum. Compound 2 was also found in the leaves of this species, while 1 was obtained from the leaves of E. nummularia. Compound 1 showed insecticidal activity against Aedes aegypti larvae.
Asunto(s)
Aedes/efectos de los fármacos , Diterpenos/química , Erythroxylaceae/química , Insecticidas/química , Animales , Diterpenos/aislamiento & purificación , Frutas/química , Insecticidas/aislamiento & purificación , Larva/efectos de los fármacos , Resonancia Magnética Nuclear Biomolecular , Extractos Vegetales/química , Hojas de la Planta/química , Pruebas de ToxicidadRESUMEN
Extracts of 32 plants from the Brazilian northeastern semi-arid region called Caatinga were evaluated through DPPH radical scavenging assay, beta-carotene bleaching, and brine shrimp lethality tests (BST). Among the extracts studied Byrsonima cf. gardneriana, Mascagnia coriacea, Cordia globosa, Diodia apiculata and Hypenia salzmannii showed the highest activities in DPPH radical scavenging test. In the beta-carotene bleaching test the highest activities were observed for Passiflora cincinnata, Chamaecrista repens, B. cf. gardneriana, Rollinia leptopetala, Serjania glabrata, Diospyros gaultheriifolia, C. globosa, Mimosa ophtalmocentra, M. coriacea and Lippia cf. microphylla. In contrast, R. leptopetala, Zornia cf. brasiliensis and Leonotis nepetifolia were the most active species in the BST.
Asunto(s)
Antioxidantes/farmacología , Depuradores de Radicales Libres/farmacología , Fitoterapia , Extractos Vegetales/farmacología , Plantas Medicinales , Animales , Antioxidantes/administración & dosificación , Antioxidantes/uso terapéutico , Artemia/efectos de los fármacos , Compuestos de Bifenilo , Brasil , Depuradores de Radicales Libres/administración & dosificación , Depuradores de Radicales Libres/uso terapéutico , Concentración 50 Inhibidora , Dosificación Letal Mediana , Medicina Tradicional , Picratos/química , Extractos Vegetales/administración & dosificación , Extractos Vegetales/uso terapéuticoRESUMEN
Two new isoflavonoids 7,8,4'-trimethoxyisoflavone and 7,8,4'-trimethoxyisoflavanone and calycosin (7,3'-hydroxy-4'-methoxyisoflavone) were isolated from the wood of the leguminous tree Bowdichia virgilioides by usual chromatographic procedures. Besides these compounds the pterocarpane (-)-maackianin, isoliquiritigenin (4,2',4'-trihydroxychalcone), and the hydrobenzylfurane derivative bowdenol were also obtained. The structures of these new compounds were determinated by MS and 1D and 2D NMR spectral analysis.
Asunto(s)
Fabaceae/química , Flavanonas/aislamiento & purificación , Isoflavonas/aislamiento & purificación , Flavanonas/química , Isoflavonas/química , Espectroscopía de Resonancia Magnética , Espectrometría de Masa por Ionización de ElectrosprayRESUMEN
This work describes the isolation and quantification of rotenoids from crude organic extracts of different parts of Clitoria fairchildiana R. A. Howard (Leguminosae) by HPLC-DAD. The lipid composition and the Artemia salina cytotoxic activities of the isolates were also conducted. Clitoriacetal (1), 6-deoxyclitoriacetal (2), stemonal and stemonone were isolated by chromatographic procedures and identified by usual spectroscopic and spectrometric techniques. Clitoriacetal and 6-deoxyclitoriacetal were not found in all parts of the plant, such as leaves and petals, but in the roots they occur in higher concentration. The activity against brine shrimp revealed that the root extract (LD50 = 158 ppm) was the more active.
Asunto(s)
Clitoria/química , Rotenona/análogos & derivados , Animales , Artemia , Clitoria/toxicidad , Lípidos/análisis , Rotenona/análisisRESUMEN
The bark of Mimosa tenuiflora (Willd.) Poiret (Leguminosae family), popularly known as "jurema preta" in Brazil, is used by the population of Contendas of Sincorá (Bahia State, Brazil) for the treatment of coughs and wound healing. Thus, the aim of this study was to evaluate the antinociceptive and anti-inflammatory activities of the bark ethanol extract (EEMT) and solvent soluble fractions (hexane-H, DCM-D, EtOAc-E and BuOH-B) of the extract in vivo. Additionally, we synthesized 5,7-dihidroxy-4'-methoxyflavanone (isosakuranetin) and isolated the compound sakuranetin, and both compounds were also tested. The anti-inflammatory and antinociceptive assays performed were: writhing test; nociception induced by intraplantar formalin injection; leukocyte recruitment to the peritoneal cavity; evaluation of vascular permeability (Evans blue test); and evaluation of mechanical hypernociception (von Frey test). Production of TNF-α, IL-10, myeloperoxidase and the expression of ICAM-1 were also evaluated. Statistical analysis was performed by one-way ANOVA followed by the Bonferroni post-test (n = 8), with P < 0.05. The EEMT showed antinociceptive activities in writhing test (100-200 mg/kg), in the second phase of the formalin test (50-200 mg/kg), and in mechanical hypernociception (100 mg/kg). EEMT showed an anti-inflammatory effect by reducing neutrophil migration to the peritoneal cavity and in the plantar tissue detected by the reduction of myeloperoxidase activity (100 mg/kg), reduction of IL-10 levels and expression of ICAM-1 in the peritoneal exudate and the mesentery (100 mg/kg), respectively. The four soluble EEMT fractions showed good results in tests for antinociceptive (H, D, E, B) and anti-inflammation (H, D, E). Only sakuranetin showed reduction of the writhing and neutrophil migration (200 mg/kg). Thus, the EEMT and soluble fractions of M. tenuiflora bark demonstrated great antinociceptive and anti-inflammatory activities, as also sakuranetin. More studies should be conducted to elucidate the mechanism of action of this compound. To the best of our knowledge, this is the first report on the antinociceptive activity of the M. tenuiflora fractions and the bioactive isolated compound sakuranetin in vivo.
Asunto(s)
Analgésicos/farmacología , Antiinflamatorios/farmacología , Flavonas/farmacología , Mimosa/química , Extractos Vegetales/farmacología , Analgésicos/química , Analgésicos/aislamiento & purificación , Animales , Antiinflamatorios/química , Antiinflamatorios/aislamiento & purificación , Permeabilidad Capilar/efectos de los fármacos , Quimiotaxis de Leucocito/efectos de los fármacos , Citocinas/biosíntesis , Edema/inducido químicamente , Edema/tratamiento farmacológico , Flavonas/química , Flavonas/aislamiento & purificación , Expresión Génica , Hiperalgesia/inducido químicamente , Hiperalgesia/tratamiento farmacológico , Molécula 1 de Adhesión Intercelular/genética , Molécula 1 de Adhesión Intercelular/metabolismo , Masculino , Ratones , Neutrófilos/efectos de los fármacos , Neutrófilos/fisiología , Peroxidasa/metabolismo , Extractos Vegetales/químicaRESUMEN
An unusually substituted coumarin, named poligalen, was isolated from a chloroform extract of the aerial parts of Polygala boliviensis. This coumarin was identified by one- and two-dimensional NMR techniques, and the structure of the compound was confirmed by X-ray diffraction. Poligalen exhibits immunomodulatory effects, reducing the levels of IL-6 and TNF after LPS stimulation in peritoneal macrophages. However, poligalen potentiates NF-kB activation.
Asunto(s)
Cumarinas/química , Interleucina-6/metabolismo , Macrófagos Peritoneales/efectos de los fármacos , Polygala/química , Factor de Necrosis Tumoral alfa/metabolismo , Animales , Cumarinas/aislamiento & purificación , Regulación hacia Abajo , Masculino , Ratones , Ratones Endogámicos C57BL , Estructura Molecular , FN-kappa B/metabolismo , Componentes Aéreos de las Plantas/química , Cultivo Primario de Células , Células RAW 264.7RESUMEN
The new triterpene Delta1-lupenone (1), together with lupeol, beta-amyrin and betulin were isolated from the wood of Byrsonima microphylla (Malpighiaceae). The new compounds 3-hydroxy-2-methoxy-8,8,10-trimethyl-8H-antracen-1,4,5-trione (2), 3,7-dihydroxy-2-methoxy-8,8,10-trimethyl-7,8-dihydro-6H-antracen-1,4,5-trione (3), (2S*,10aR*)-2,8-dihydroxy-6-methoxy-1,1,7-trimethyl-2,3,10, 10a-tetrahydro-1H-fenantren-9-one (4) and (2S,3S)-3'-hydroxy-4',5,7-trimethoxy-flavan-3-ol (5) were also isolated through monitored TLC using the antioxidant beta-carotene reagent. The antioxidant potential of the compounds 2-5 was measured and none of them showed activity. The structures of these compounds were elucidated by chemical and spectroscopic analysis based on NMR techniques (1H, 13C NMR, COSY, nOe difference, HMQC and HMBC), UV and MS.
Asunto(s)
Catequina/aislamiento & purificación , Malpighiaceae/química , Naftoquinonas/aislamiento & purificación , Triterpenos/aislamiento & purificación , Catequina/química , Radicales Libres/química , Espectroscopía de Resonancia Magnética , Estructura Molecular , Naftoquinonas/química , Tallos de la Planta/química , Triterpenos/químicaRESUMEN
The ethyl acetate extract of leaves of Moldenhawera nutans Queiroz & Alkin (Leguminosae) furnished, besides methyl gallate and gallic acid, the flavonols named laricetrin, laricetrin 3-glucoside and laricetrin 3-galactoside as well as the new one named laricetrin 5-galloyl-3-beta-D-xylopyranoside. It also was isolated from the hexane extract: beta-sitosterol, lupenone, beta-amyrinone, alpha-amyrinone, lupeol, beta-amyrin, alpha-amyrin and alpha-tocopherol. The antioxidant activities of flavonoids were measured through DPPH radical scavenging and inhibition of auto-oxidation of beta-carotene methods. The structures of the compounds were determined by analyses of spectral data. This is the first report dealing with phytochemical studies of leaves of M. nutans. In addition this current work describes the unequivocal attribution of 1H NMR and 13C NMR data of laricetrin.
Asunto(s)
Antioxidantes/química , Extractos Vegetales/química , Hojas de la Planta/química , Antioxidantes/aislamiento & purificación , Antioxidantes/farmacología , Brasil , Depuradores de Radicales Libres , Espectroscopía de Resonancia Magnética , Extractos Vegetales/aislamiento & purificación , Extractos Vegetales/farmacología , Tocoferoles/química , Tocoferoles/aislamiento & purificación , Clima TropicalRESUMEN
Phytochemical investigation of the trunk of Schinopsis brasiliensis afforded a new alkyl phenol, methyl 6-eicosanyl-2-hydroxy-4-methoxybenzoate, besides an unusual steroid 5alpha,8alpha-epidioxyergosta-6,22-dien-3-beta-ol. The compounds were characterised by spectroscopy data analysis.
Asunto(s)
Anacardiaceae/química , Benzoatos/química , Eicosanoides/química , Ergosterol/análogos & derivados , Ergosterol/química , Estructura MolecularRESUMEN
From the fruits of Allophylus laevigatus a new sesquiterpene, 11-acetoxy-4 alpha-methoxyeudesmane, was isolated alongwith the known compounds carissone and apigenin-8-C-beta-rhamnopyranoside. The flavone showed no antioxidant activity in the autoxidation of beta-carotene assay.
Asunto(s)
Fitoterapia , Extractos Vegetales/química , Sapindaceae , Sesquiterpenos/química , Frutas , Humanos , Espectroscopía de Resonancia MagnéticaRESUMEN
Neoflavonoids comprise a group of natural compounds with varied chemical structures and promising pharmacological properties, including antioxidant capacity. This work describes an evaluation of the in vitro antioxidant capacity of a new coumarin derivative, i.e., 7-acetoxy-4-aryl-3,4-dihydrocoumarin, in terms of its ability to quench the 2,2- diphenyl-1-picrylhydrazyl (DPPHâ¢), 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTSâ¢+), hydroxyl (OHâ¢) and superoxide anion (O2(â¢-)) radicals, as well as its capacity to initiate electron transfer by reducing potential and inhibit lipid peroxidation by TBARS (thiobarbituric acid reactive substances) method. In addition, the antioxidant capacity of 7-acetoxy-4-aryl-3,4-dihydrocoumarin was evaluated against oxidative damage induced by hydrogen peroxide in erythrocyte suspensions and S. cerevisiae strains. In all methodologies investigated, high antioxidant capacities above 65% were demonstrated by 7-acetoxy-4-aryl-3,4-dihydrocoumarin against the DPPH(â¢), ABTS(â¢+), OH(â¢) and O2(â¢-) radicals. The ability of 7-acetoxy-4-aryl-3,4-dihydrocoumarin to inhibit oxidative damage induced by hydrogen peroxide in erythrocytes and S. cerevisiae strains demonstrates the importance of this compound in the protection against oxidative stress at the cellular level. Thus, the results obtained in this study suggest that 7-acetoxy-4-aryl-3,4-dihydrocoumarin can assist the development of new antioxidant products for possible use in the prevention or reduction of diseases related to oxidative stress.
Asunto(s)
Antioxidantes/farmacología , Cumarinas/farmacología , Eritrocitos/efectos de los fármacos , Radicales Libres/química , Radicales Libres/metabolismo , Estrés Oxidativo/efectos de los fármacos , Saccharomyces cerevisiae/efectos de los fármacos , Animales , Antioxidantes/síntesis química , Antioxidantes/química , Benzotiazoles/química , Compuestos de Bifenilo/química , Cumarinas/síntesis química , Cumarinas/química , Relación Dosis-Respuesta a Droga , Eritrocitos/metabolismo , Hemólisis/efectos de los fármacos , Peroxidación de Lípido/efectos de los fármacos , Picratos/química , Ratas , Saccharomyces cerevisiae/metabolismo , Ácidos Sulfónicos/químicaRESUMEN
The present study primarily aims to identify the relative density and the fatty acids (methyl esters) content present in the standardized ethanol extract of leaves of M. glomerata (EPMG). Meanwhile, in a second moment, this study evaluated the effects of the EPMG on the levels of amino acids in the hippocampus, and the mechanism of sedative and anxiolytic action. Adult mice were treated with doses of 200, 300, and 400 mg/kg and evaluated in open field, elevated plus-maze, light dark, and rotarod tests. Moreover, in the behavioral tests diazepam (GABAergic anxiolytic, 2 mg/kg) as positive control and flumazenil (GABA antagonist, 2.5 mg/kg) were used to identify mechanism of sedative and anxiolytic action produced by EPMG. The EPMG is constituted by the following compounds: methyl cinnamate, 2H-1-benzopyran-2-one, (2-hydroxyphenyl)methyl propionate, (Z)-methyl-hexadec-7-enoate, methyl hexadecanoate, hexadecanoic acid, (Z)-methyl-octadec-9-enoate, octadecanoic acid, and squalene. This extract demonstrated anxiolytic effects, which may be mediated by GABAergic system, and was able to increase GABA levels and reduce of glutamate and aspartate concentrations in mice hippocampus, which can directly and/or indirectly assist in their anxiolytic effect. Although more studies are needed, the EPMG could represent an interesting therapeutical strategy in the treatment of anxiety.
RESUMEN
Flavanones (hesperidin, naringenin, naringin, and poncirin) in industrial, hand-squeezed orange juices and from fresh-in-squeeze machines orange juices were determined by HPLC/DAD analysis using a previously described liquid-liquid extraction method. Method validation including the accuracy was performed by using recovery tests. Samples (36) collected from different Brazilian locations and brands were analyzed. Concentrations were determined using an external standard curve. The limits of detection (LOD) and the limits of quantification (LOQ) calculated were 0.0037, 1.87, 0.0147, and 0.0066 mg 100 g(-1) and 0.0089, 7.84, 0.0302, and 0.0200 mg 100 g(-1) for naringin, hesperidin, poncirin, and naringenin, respectively. The results demonstrated that hesperidin was present at the highest concentration levels, especially in the industrial orange juices. Its average content and concentration range were 69.85 and 18.80-139.00 mg 100 g(-1). The other flavanones showed the lowest concentration levels. The average contents and concentration ranges found were 0.019, 0.01-0.30, and 0.12 and 0.1-0.17, 0.13, and 0.01-0.36 mg 100 g(-1), respectively. The results were also evaluated using the principal component analysis (PCA) multivariate analysis technique which showed that poncirin, naringenin, and naringin were the principal elements that contributed to the variability in the sample concentrations.