RESUMEN
The comparative analysis of genetic differentiations between three species of Bovinae--Bos taurus, Bison bonasus, Bison bison with the use of different types of molecular-genetic markers--genetical-biochemical (35 loci) and DNA markers (RAPD-PCR, ISSR-PCR) was carried out. It was shown, that the evaluation of interspecies genetic interrelations was connected more with the determined molecular-genetic markers (loci), included in analysis, than with the marker's belonging to certain type (protein polymorphism, variability of DNA repeat distributions).
Asunto(s)
Bison/genética , Proteínas Sanguíneas/genética , Bovinos/genética , Polimorfismo Genético/genética , Alelos , Animales , Bison/sangre , Bovinos/sangre , Electroforesis en Gel de Poliacrilamida , Electroforesis en Gel de Almidón , Marcadores Genéticos/genética , Reacción en Cadena de la Polimerasa/métodos , Reacción en Cadena de la Polimerasa/estadística & datos numéricos , Técnica del ADN Polimorfo Amplificado Aleatorio , Secuencias Repetitivas de Ácidos Nucleicos , Especificidad de la EspecieRESUMEN
We have developed diagnostic system for revealing 35S promoter CaMV and transgene CryIIIA in transgenic potato NL Russet Burbank and NL Superior companies Monsanto. Using the data about nucleotide sequences of 35S promoter (AF234316 GenBank) and gene CryIIIA (X70979 GenBank) oligonucleotides primers were synthesized for polymerase chain reaction (PCR). The technique of genomic DNA isolation from plant material, suitable for PCR has been optimized. Using Touchdown PCR method presence of specific transgene in a Bt-potato was shown. This method can be used in routine laboratory practice for revealing 35S promoter in genetically modified plants and for identification of specific transgene CryIIIA in the transformed Bt-plants.
Asunto(s)
Toxinas Bacterianas , Genes de Plantas , Plantas Modificadas Genéticamente/genética , Solanum tuberosum/genética , Toxinas de Bacillus thuringiensis , Proteínas Bacterianas/genética , Cartilla de ADN/biosíntesis , Endotoxinas/genética , Proteínas Hemolisinas , Virus del Mosaico/genética , Reacción en Cadena de la Polimerasa/métodos , Regiones Promotoras Genéticas , TransgenesRESUMEN
Localization of virus RNA in stroma of Capsicum anuum L. chloroplasts was determined by the PCR method. Accumulation of virus protein in the membranes and stroma of infected pepper chloroplasts has been studied. It is concluded that the virus protein synthesis takes place in the pepper chloroplasts.