Stable carbon and nitrogen isotope inter- and intra-individual dietary reconstruction from the late 14th to early 18th century site of Alytus, Lithuania.

OBJECTIVES: The Baltic region, particularly Lithuania, was a politically vibrant area of Eastern Europe during the medieval and early modern period. To better understand the diet of Lithuanians during the late 14th to early 18th century, we examine stable carbon and nitrogen isotope ratios from bone and dentin samples from the site of Alytus. We investigate possible dietary differences based on sex, age, and religious practice, as well as dietary changes throughout an individual's lifetime, within the broader European milieu. MATERIALS AND METHODS: Stable carbon and nitrogen isotope analysis was conducted on bone (n = 35) and dentin (n = 38) collagen samples from a total of 39 individuals buried in the cemetery at Alytus (late 14th to early 18th centuries). RESULTS: Results indicate individuals at Alytus consumed a C3 terrestrial based diet. The δ13 C and δ15 N values are not significantly different between bone and dentin, and did not vary by sex. DISCUSSION: The diet at Alytus was homogeneous between males and females and between tissue types. The lack of evidence indicating substantial consumption of fish is unexpected given widespread Catholic fasting practices and marine resource trade throughout Europe. Comparisons with other populations indicate that individuals from Alytus differ in diet from contemporaneous Polish-Lithuanian Commonwealth elites. Comparison of the diets of non-elite individuals in the Eastern Baltic region also reveals dietary variability.

Reconstructing diet in Napoleon's Grand Army using stable carbon and nitrogen isotope analysis.

OBJECTIVES: Historical evidence has provided information regarding disease and mortality in Napoleon Bonaparte's Grand Army, but dietary information beyond individual soldier accounts remains scarce. The purpose of this research is to reconstruct the diets of Napoleon's multiethnic army who were associated with the Russian Campaign of 1812. MATERIALS AND METHODS: We conducted stable carbon and nitrogen isotope ratio analysis on femoral bone collagen of 78 individuals recovered from a salvage excavation at the mass gravesite of Siaures miestelis in Vilnius, Lithuania. These individuals were later discovered to be Napoleonic soldiers and camp followers who participated in the 1812 Russian Campaign. RESULTS: Stable carbon isotope ratios range from -19.2‰ to -11.8‰, with a mean of -17.8‰ ± 1.5‰ (1 σ). Stable nitrogen isotope ratios range from 7.1‰ to 13.6‰, with a mean of 10.5‰ ± 1.4‰ (1 σ). Both δ13 C and δ15 N values show a wide range of variation. DISCUSSION: Stable isotope data indicate considerable dietary variation in this population associated with a multiethnic and socially stratified military population. Diets ranged from predominantly C3 -based to predominantly C4 -based, with varying inputs of terrestrial, freshwater, and marine animal protein. Comparison with other European populations further denotes the exceptional range of dietary variation of soldiers and camp followers in Napoleon's army.

Growth of the pectoral girdle in a sample of juveniles from the kellis 2 cemetery, Dakhleh Oasis, Egypt.

OBJECTIVES: This study investigates growth patterns in the scapula and clavicle in a cross-sectional juvenile skeletal sample ranging from 20 weeks gestation to 8.5 years of age from the Kellis 2 cemetery, Dakhleh Oasis, Egypt. The primary goal is to quantify growth patterns and growth velocities in the scapula and clavicle to better understand the development of the pectoral girdle. METHODS: A series of low-order polynomial regression models was used to examine growth curves in clavicle diaphyseal length, scapular height, and scapular width. Incremental growth and relative percent increase were examined among successive age groups as a proxy measure of growth velocity. Scapular body proportions were assessed with the scapular index and compared across age groups using a Kruskal-Wallis test with post-hoc tests. RESULTS: A third-order polynomial best describes growth in clavicle diaphyseal length and scapular height, and a second-order polynomial best describes growth in scapular width. Growth velocity patterns are similar among clavicle diaphyseal length, scapular height, and scapular width particularly from birth until the end of early childhood. Clavicle diaphyseal length decelerates during middle childhood while scapular height and width accelerate during this time. With increasing age, the scapular body proportionately increases more in height than in width. The relatively narrow scapular body characteristic of adult scapulae is first evident during early childhood. CONCLUSIONS: Changes in scapular body shape during ontogeny may be a reflection of the greater alterations taking place in the integrated morphology of the pectoral girdle during the biomechanical shift from crawling to bipedalism. Am. J. Hum. Biol. 28:636-645, 2016. © 2016 Wiley Periodicals, Inc.

An exploration of adult body shape and limb proportions at Kellis 2, Dakhleh Oasis, Egypt.

Several studies have shown that the human body generally conforms to the ecogeographical expectations of Bergmann's and Allen's rules; however, recent evidence suggests that these expectations may not hold completely for some populations. Egypt is located at the crossroads of Sub-Saharan Africa, Southern Europe, and the Near East, and gene flow among groups in these regions may confound ecogeographical patterning. In this study, we test the fit of the adult physique of a large sample (N = 163) of females and males from the Kellis 2 cemetery (Dakhleh Oasis, Egypt) against ecogeographical predictions. Body shape (i.e., body mass relative to stature) was assessed by the femur head diameter to bicondylar femur length index (FHD/BFL), and brachial and crural indices were calculated to examine intralimb proportions. Body shape in the Kellis 2 sample is not significantly different from high-latitude groups and a Lower Nubian sample, and intralimb proportions are not significantly different from mid-latitude and other low-latitude groups. This study demonstrates the potential uniqueness of body shape and intralimb proportions in an ancient Egyptian sample, and further highlights the complex relationship between ecogeographic patterning and adaptation.

Ontogenetic changes in intralimb proportions in a Romano-Christian period sample from the Dakhleh Oasis, Egypt.

OBJECTIVES: The purpose of this study is to document the appearance of adult patterns in intralimb indices during ontogeny in a skeletal sample from the Kellis 2 cemetery, Dakhleh Oasis, Egypt. In addition, this study explores evolvability in intralimb indices to understand relative differences in sensitivity to ecogeographic variables. METHODS: Brachial and crural indices were compared across age cohorts with Welch's ANOVA tests and post-hoc Dunnett-Tukey-Kramer (DTK) pairwise multiple comparison tests. Spearman's rank correlation coefficients were used to examine developmental conservation and evolvability in intralimb proportions. RESULTS: Brachial and crural indices are greatest in the fetus/perinate cohort as compared to all other cohorts, decrease during infancy and early childhood, and increase during middle/late childhood. The adult pattern in the brachial index is first evident in infancy, but is not maintained throughout development. Conversely, the adult pattern in the crural index appears during early childhood and is maintained throughout development. The brachial index shows a higher degree of evolvability than the crural index in utero. CONCLUSIONS: The shifting pattern in intralimb proportions during development in the Kellis 2 sample is similar to that previously reported from globally diverse samples, which likely reflects the differential growth acceleration of proximal and distal intralimb skeletal elements during ontogeny. The brachial index may be more responsive to climatic conditions while the crural index may be more conserved due to functional demands. The data indicate that Kellis 2 juveniles were under strong selective pressures from climatic factors.

Determination of sex from the hyoid bone.

This article explores size differences related to sex in the hyoid bones from the Robert J. Terry Anatomical Collection. A series of measurements were taken from 398 hyoids, both fused and unfused. The inclusion of unfused hyoids in the study provides the opportunity to investigate previously unknown size differences between sexes as well as to determine their utility in determining sex. Two-way ANOVA was used to explore differences in hyoid size as related to ancestry and sex. Discriminant function analysis was employed to test the ability of the hyoids to be classified by sex. Six discriminant function equations ranging in accuracy from 82% to 85% are provided, each of which is more accurate than many of the discriminant functions developed in past hyoid research, are simple to use, and can be used to estimate the sex of a hyoid regardless of its state of fusion. In addition to providing further information about the morphological form of the hyoid, these analyses provide a method that can be easily employed to assess sex of the individual from the hyoid bone.

Shattered lives and broken childhoods: Evidence of physical child abuse in ancient Egypt.

Much can be learned about cultural attitudes of violence towards children from the analyses of their skeletal remains and mortuary patterns of the communities in which they lived and died. A bioarchaeological approach integrating biological, socio-cultural, and physical environments is used in analyzing the remains of a 2-3-year-old child from Kellis 2, a Romano-Christian period cemetery in the Dakhleh Oasis, Egypt. The skeletal remains of this individual show an unusual pattern of trauma and healing events, possibly indicating multiple episodes of non-accidental trauma. Macroscopic, radiographic, and histologic analyses show the extent of the skeletal trauma and healing, while stable carbon and nitrogen analyses of bone and hair reveal metabolic disturbances and changes in diet correlated with these traumatic events. Results from the differential diagnosis demonstrate that this individual exhibits skeletal fracture and healing patterns consistent with repeated non-accidental trauma, which may or may not have resulted in death. In addition, this individual may also represent the earliest documented case of violence against children from an archaeological context.

Exploring ancestral variation of the hyoid.

This study utilizes metric analysis to examine size and shape variation between hyoids of Africans and Europeans in the Robert J. Terry Anatomical Collection. A total of 200 fused and unfused hyoids were measured and three statistical methods were employed to explore variation between ancestries. First, independent sample t-tests showed that some significant size differences do occur between ancestries. Second, to examine shape variation, skeletal measurements were regressed on the geometric mean using least squares linear regression with the residuals used to evaluate size-corrected shape differences. Finally, discriminant function analysis was used to develop two functions for ancestry prediction with overall accuracies of 73% and 77%. Results of the analyses suggest hyoid size and shape differences do occur between ancestries, notably that European hyoids are broader than African hyoids, while the African hyoid is longer than Europeans.

Osteogenesis imperfecta in the archeological record: An example from the Dakhleh Oasis, Egypt.

The present study evaluates a fetal skeleton (B532) from the Romano-Byzantine period Kellis 2 cemetery (circa A.D. 50-A.D. 450), in the Dakhleh Oasis, Egypt. This skeleton displays abnormal skeletal characteristics consisting of severe bowing of the long bones. Differential diagnoses using macroscopic and radiographic analyses indicate that this individual's pathological condition was caused by osteogenesis imperfecta (IO), possibly Type IIB/III, or Type IV OI, corroborating with characteristics reported in the clinical literature. Due to the severe bowing of the long bones, traditional aging methods could not be used for this individual. Baysian estimates of age indicate this individual was a fetus of approximately 38 weeks gestation. Although this condition has been diagnosed previously in the archeological literature, this burial represents the youngest aged example of osteogenesis imperfecta reported to date.

The effects of household corrosive chemicals on human dentition.

There is a gap in the literature concerning the chemical effects that household products may produce on human remains. The present study examines the effects of household chemical products on teeth. A total of eight chemicals were utilized for this experiment. The corrosive chemical categories include: hydrochloric acid, sulfuric acid, phosphoric acid, and sodium hydroxide. Two products with each chemical were used, each representing varying concentrations of the corrosive product. Two human teeth were allocated for emergence in the chemical throughout a 24-h period of exposure. Results demonstrate hydrochloric acid as the most detrimental chemical to the dental samples. Sulfuric acid enacted minimal alterations to the teeth, although some etching and discoloration were noticeable. Phosphoric acid resulted in variable changes of the organic and inorganic contents of teeth. Lastly, exposure of sodium hydroxide resulted in little to no change. As hypothesized, distinct effects are observable of each chemical.

Reconstructing infant weaning histories at Roman period Kellis, Egypt using stable isotope analysis of dentition.

Studies of infant feeding and weaning patterns in past populations that rely on a cross-sectional approach must make the assumption that no infant mortality bias exists. Previous investigations of infant weaning patterns at the Dakhleh Oasis, Egypt, relied on cross-sectional isotope data. In this study, we re-examine this weaning pattern, using a simulated longitudinal approach, which does not require any assumptions regarding potential infant mortality biases. This involves examining the dental isotopic signatures of individuals who survived the weaning process. Stable isotope signatures from juveniles and adults (102 individuals, 297 teeth) were examined to reconstruct the weaning history of those that survived the weaning process. Both deciduous and permanent teeth were sampled. Homogenized enamel and dentin samples were isolated from each tooth and analyzed for delta(13)C(ap) and delta(18)O(ap) from the enamel and delta(15)N(coll) and delta(13)C(coll) from dentin collagen. We investigate differences between in utero versus postbirth, preweaning versus postweaning, and juvenile versus adult stable isotope values as reflected in the dentition. A random permutation procedure was used to test for statistically significant differences in stable isotope values between tooth types. Statistically significant differences were observed in all stable isotopes between permanent and deciduous teeth, and between early and later forming permanent teeth in delta(13)C(ap) and delta(15)N(coll) isotopes. These results indicate dietary change between in utero and postbirth, and changes occurring during the weaning period. These results provide a more comprehensive picture of infant weaning practices at Kellis and provide further support that complete weaning occurred by 3 years of age.