Effect of Solanum lycopersicum and Citrus limon-Derived Exosome-Like Vesicles on Chondrogenic Differentiation of Adipose-Derived Stem Cells.

Articular cartilage defect treatment is a very important problem because its therapeutic options are not successful enough. Due to the weak self-repairing capacity of the avascular cartilage, even minor damage can progress and cause joint damage leading to osteoarthritis. Although various treatment strategies have been developed to repair damaged cartilage, cell- and exosome-based therapies are promising. Plant extracts have been used for decades, and their effects on cartilage regeneration have been studied. Exosome-like vesicles, which are secreted by all living cells, are involved in cell-to-cell communication and cell homeostasis. The differentiation potential of exosome-like vesicles isolated from S. lycopersicum and C. limon, which are known to have anti-inflammatory and antioxidant properties, was investigated in the differentiation of human adipose-derived mesenchymal stem cells (hASCs) into chondrocytes. In order to obtain tomato-derived exosome-like vesicles (TELVs) and lemon-derived exosome-like vesicles (LELVs) Aquous Two- Phase system was performed. Characterisation of isolated vesicles based on size, shape were achived via Zetasizer, NTA FAME analysis, and SEM techniques. These results showed that TELVs and LELVs increased cell viability and did not show any toxic effects on stem cells. Although TELVs triggered chondrocyte formation, LELVs downregulated. The expression of ACAN, SOX9, and COMP, known as chondrocyte markers, was increased by TELV treatment. In addition, protein expression of the two most important proteins, COL2 and COLXI, found in the extracellular matrix of cartilage, increased. These findings suggest that TELVs can be used for cartilage regeneration, and may be a novel and promising treatment for osteoarthritis.

Is Mediolateral Translation of Femur Caused by Total Knee Arthroplasty Being Ignored?

Purpose: This study aims to show the change in overall congruency due to mediolateral translation after total knee arthroplasty compared with normal knee anatomy. Methods: This study was performed in two parts. In part 1, the relationship between femur and tibia was defined by new parameters on the antero-posterior radiographs of 84 patients. In part 2, this relationship was evaluated on the postoperative radiographs of 136 total knee arthroplasty patients. Two parallel lines to the tibial anatomical axis were drawn tangent to the most lateral and most medial parts of the tibial plateau. After creating medial and lateral tangential lines, the distance between the most lateral point of the lateral femoral epicondyle and lateral tangential line and the most medial point of the medial femoral epicondyle and medial tangential line was measured. Another new parameter described in the study is epicondylar distance ratio. The ratios between the shortest distance between tibial anatomical axis and lateral femoral epicondyle and the distance between tibial anatomical axis and medial femoral epicondyle were defined. Results: It was found that the lateral tangent was not superposed in any measurement to the femoral lateral condyle, the closest tangent was passed, and the mean lateral space distance was 1.8 mm (SD 1.5, 95% CI 0-5.3 mm). The medial tangent was passed from the lateral to the femoral medial epicondyle, and the medial crossing distance was 8.5 mm (SD 5.7, 95% CI 5-14 mm). Epicondylar distance ratio used as the second measurement was 0.8 (0.5-0.9). After total knee arthroplasty measurements showed that the line passing through the lateral tibia crossed the lateral epicondyle of the femur and intersected at an average distance of 4.3 mm (SD 4.1, 95% CI 1-11.2 mm). Conclusions: There is a coronal plane congruence between tibia and femur in the healthy knees, which get changed after total knee arthroplasty.