RESUMEN
To assess the effect of aluminium intoxication in tissues of experimental animals on Ca, Mg, Zn, Cu, and Fe concentration, aluminium nitrate was administered intraperitoneally to mice at a daily dose of 0.27 mMol/kg for 5 weeks. Concentration of Al, Ca, Mg, Zn, Cu, and Fe were analyzed by atomic absorption spectrometry. The Al content in liver and tibia was significantly higher in treated mice in comparison with control group. In Al loaded tissues the significant increase of all tested essential elements was found. To evaluate the results of DFO treatment on essential elements, mice received 6 times intraperitoneally 3.5 mMol/kg of DFO. This treatment had generally no effect on reduction of Al concentration in tibia and liver, as well as on changes in essential elements concentration.
Asunto(s)
Compuestos de Aluminio/toxicidad , Deferoxamina/farmacología , Metales/metabolismo , Nitratos/toxicidad , Animales , Calcio/metabolismo , Cobre/metabolismo , Inyecciones Intraperitoneales , Hierro/metabolismo , Hígado/química , Magnesio/metabolismo , Metales/análisis , Ratones , Tibia/química , Zinc/metabolismoRESUMEN
The present work contains information about proper husbandry and care of laboratory animals, microbiological monitoring of their health and protecting them against suffering and distress. The author also gives some advice on the improvement and unification of experimental research results through the standardisation of laboratory animals used for the experiments as well as imposing proper conditions for animal husbandry.
Asunto(s)
Crianza de Animales Domésticos , Animales de Laboratorio , Anestesia , Bienestar del Animal/legislación & jurisprudencia , Animales , Animales de Laboratorio/clasificación , Animales de Laboratorio/genética , Animales de Laboratorio/microbiología , Recolección de Muestras de Sangre , Eutanasia , Femenino , Control de Infecciones , Masculino , Ratones , Ratas , Proyectos de Investigación/legislación & jurisprudencia , Estrés Fisiológico/prevención & controlRESUMEN
Congenital rubella is a clinically serious problem, which is due to maternal infection with rubella virus during the first trimester of pregnancy. It may result in spontaneous abortion or in fetal infection leading to fetal birth defect. Maternal anti-rubella antibodies have a protective effect and they may prevent congenital rubella. Vaccination of 13-year-old girls in Poland was introduced in 1989. Vaccination coverage is above 95%. The presence of anti-rubella antibodies in maternal blood >15 IU/ml is indicative for immunity to rubella. Thus, the aim of the study was the evaluation of anti-rubella antibody levels in women 15-30 years old. Samples of that sera were collected from six provinces in Poland. We have observed the decrease of anti-rubella antibody titer together with increasing age of tested women. Among 1289 investigated women 135 [10.5%] did not have protective levels of anti-rubella antibodies. We have noticed large decline in protective anti-rubella antibodies in young women (19-22 years of age), who are just entering their prime childbearing years.