[Fornix reconstruction with conjunctival inclusion cyst marsupialization in severe symblepharon].

Objective: To report the efficacy of fornix reconstruction with conjunctival inclusion cyst marsupialization in severe symblepharon. Methods: Retrospective cases series study. Four patients (4 eyes) with Conjunctival Inclusion Cyst Marsupialization and severe Symblepharon during June 2016 to June 2018 at the PLA General Hospital were included. All of them were treated with Conjunctival Inclusion Cyst Marsupialization. The morphology, depth of the conjunctival sac and the degree of symblepharon before and after treatment were compared to observe the surgical results. Results: After operation, the conjunctival sacs were deepened, and the average depth was 4.75 mm. After follow-up, 2 cases of conjunctival sac were basically normal, and 2 cases of conjunctival sac became shallow, but still better than before surgery. Conclusions: For the patients with large Conjunctival Inclusion Cyst and severe symblepharon, the Conjunctival Inclusion Cyst Marsupialization have many positive effects of deepening conjunctival sac, relieving symblepharon, increasing the degree of eye movement, improving appearance and relieving dry eyes. At the same time, this operation can avoid recurrence due to incomplete cystectomy. (Chin J Ophthalmol, 2018, 54: 897-901).

Polymorphisms of the myostatin gene (MSTN) and its relationship with growth traits in goat breeds.

Mutations in the myostatin (MSTN) gene can inactivate its expression and result in a non-functional protein, which leads to dramatic muscularity and a "double-muscling" phenomenon in many species. Using gene sequencing and polymerase chain reaction-single-strand conformation polymorphism methods, polymorphisms of the MSTN gene were investigated as a candidate marker for growth in 288 goats. The results showed 2 novel single nucleotide polymorphisms: DQ167575 g.197G>A and 345A>T. Three potential genotypes (AA, AB, and BB) of substitution 197G>A in the 5'-untranslated region were detected in the 2 breeds. The polymorphism (CC and CD) of substitution 345A>T in exon I was segregated. The genetic diversity analysis revealed that Boer goat and Anhui white goat possessed intermediate genetic diversity in the P1 and P3 loci. Significant associations between the genotypes of the P3 locus and body weight, body length, and body height were observed in Boer goat and Anhui white goat (P < 0.05). It could be inferred that the MSTN gene may be a major gene or linked to the major gene affecting the goat growth traits. The polymorphic site could be a molecular marker-assisted selection program for body weight.