SIRT1/Atg5/autophagy are involved in the antiatherosclerosis effects of ursolic acid.

The purpose of this study was to investigate the antiatherosclerosis effects of ursolic acid (UA) in high-fat diet-fed quails (Coturnix coturnix) and potential mechanism. Quails were treated with high-fat diet (14 % pork oil, 1 % cholesterol w/w) with or without UA (50, 150, or 300 mg/kg/day) for 10 weeks. Serum lipid profile was assessed at 0, 4.5, and 10 weeks. After 10 weeks, serum antioxidant status and morphology of aorta were assessed. Additionally, human umbilical vein endothelial cells (HUVECs) were exposed to 100 µg/ml oxidized low-density lipoprotein (ox-LDL) for 24 h, with or without pretreatment with UA (5, 10 or 20 µM) for 16 h, autophagy inhibitor 3-MA 5 mM for 2 h, or SIRT1 inhibitor EX-527 10 µM for 2 h. Cell viability and oxidative stress status were assessed and autophagy status was determined. Acetylation of lysine residue on Atg5 was assessed with immunoprecipitation. In results, high-fat diet negatively affected serum lipid profile and antioxidant status in quails and induced significant histological changes. Cotreatment with UA remarkably alleviated such changes. In HUVECs, ox-LDL treatment induced significant cytotoxicity along with oxidative stress, while UA cotreatment alleviated such changes significantly. UA treatment induced autophagy, enhanced SIRT1 expression, and decreased acetylation of lysine residue on Atg5. Cotreatment with 3-MA or EX-527 effectively abolished UA's protective effects. In summary, UA exerted antiatherosclerosis effects in quails and protected HUVECs from ox-LDL induced cytotoxicity, and the mechanism is associated with increased SIRT1 expression, decreased Atg5 acetylation on lysine residue, and increased autophagy.

The effect of the social support on PTSD and PTG about university student volunteers in the prevention and controlling of coronavirus: with coping style as the intermediary.

To investigate the relationship among post-traumatic stress disorder (PTSD), posttraumatic growth (PTG), social support, and coping style of university student volunteers in the prevention and control of the coronavirus in 2020, a total of 2,990 university student volunteers (students who are enrolled in a university and involved in volunteer activities) from 20 universities in Sichuan Province participated in the prevention and control of the epidemic were investigated when March 20-31, 2020 when the coronavirus first occurred using the post-traumatic stress disorder questionnaire, posttraumatic growth questionnaire, university student social support questionnaire and coping style questionnaire. The results showed that (1) 7.06% of university student volunteers had some degree of PTSD symptoms (the total PCL-C score was 38-49), and 2.88% had obvious PTSD symptoms, (2) PTSD level of university student volunteers was significantly positively correlated with negative coping style, and significantly negatively correlated with social support and positive coping style; on the contrary, the PTG level is significantly positively correlated with social support and positive coping styles, and (3) Positive coping style plays a partial mediating role in the influence of social support on PTG; in the influence of social support on PTSD, the mediating effect of positive or negative coping style was not significant. These results show that in the prevention and control of the coronavirus, the positive coping style and social support of university student volunteers can positively predict the PTG level of them, while the negative coping style can positively predict the severity of their PTSD symptoms. Among them, a positive coping style plays a partial mediating role in the influence of social support on the PTG level.