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1.
Cell ; 168(1-2): 295-310.e19, 2017 Jan 12.
Artículo en Inglés | MEDLINE | ID: mdl-28041852

RESUMEN

The deep dorsal horn is a poorly characterized spinal cord region implicated in processing low-threshold mechanoreceptor (LTMR) information. We report an array of mouse genetic tools for defining neuronal components and functions of the dorsal horn LTMR-recipient zone (LTMR-RZ), a role for LTMR-RZ processing in tactile perception, and the basic logic of LTMR-RZ organization. We found an unexpectedly high degree of neuronal diversity in the LTMR-RZ: seven excitatory and four inhibitory subtypes of interneurons exhibiting unique morphological, physiological, and synaptic properties. Remarkably, LTMRs form synapses on between four and 11 LTMR-RZ interneuron subtypes, while each LTMR-RZ interneuron subtype samples inputs from at least one to three LTMR classes, as well as spinal cord interneurons and corticospinal neurons. Thus, the LTMR-RZ is a somatosensory processing region endowed with a neuronal complexity that rivals the retina and functions to pattern the activity of ascending touch pathways that underlie tactile perception.


Asunto(s)
Médula Espinal/citología , Médula Espinal/metabolismo , Sinapsis , Animales , Axones/metabolismo , Dendritas/metabolismo , Interneuronas/citología , Interneuronas/metabolismo , Mecanorreceptores/metabolismo , Ratones , Biología Molecular/métodos , Vías Nerviosas , Percepción del Tacto
2.
Cell ; 167(1): 60-72.e11, 2016 Sep 22.
Artículo en Inglés | MEDLINE | ID: mdl-27641503

RESUMEN

The frequency of human social and emotional disorders varies significantly between males and females. We have recently reported that oxytocin receptor interneurons (OxtrINs) modulate female sociosexual behavior. Here, we show that, in male mice, OxtrINs regulate anxiety-related behaviors. We demonstrate that corticotropin-releasing-hormone-binding protein (CRHBP), an antagonist of the stress hormone CRH, is specifically expressed in OxtrINs. Production of CRHBP blocks the CRH-induced potentiation of postsynaptic layer 2/3 pyramidal cell activity of male, but not female, mice, thus producing an anxiolytic effect. Our data identify OxtrINs as critical for modulation of social and emotional behaviors in both females and males and reveal a molecular mechanism that acts on local medial prefrontal cortex (mPFC) circuits to coordinate responses to OXT and CRH. They suggest that additional studies of the impact of the OXT/OXTR and CRHBP/CRH pathways in males and females will be important in development of gender-specific therapies.


Asunto(s)
Ansiedad/psicología , Proteínas Portadoras/metabolismo , Hormona Liberadora de Corticotropina/metabolismo , Interneuronas/metabolismo , Oxitocina/metabolismo , Corteza Prefrontal/metabolismo , Receptores de Oxitocina/metabolismo , Caracteres Sexuales , Animales , Ansiedad/metabolismo , Conducta Animal , Femenino , Potenciación a Largo Plazo , Masculino , Redes y Vías Metabólicas , Ratones , Factores Sexuales
3.
Genes Dev ; 37(19-20): 883-900, 2023 10 01.
Artículo en Inglés | MEDLINE | ID: mdl-37890975

RESUMEN

Loss-of-function mutations in MECP2 cause Rett syndrome (RTT), a severe neurological disorder that mainly affects girls. Mutations in MECP2 do occur in males occasionally and typically cause severe encephalopathy and premature lethality. Recently, we identified a missense mutation (c.353G>A, p.Gly118Glu [G118E]), which has never been seen before in MECP2, in a young boy who suffered from progressive motor dysfunction and developmental delay. To determine whether this variant caused the clinical symptoms and study its functional consequences, we established two disease models, including human neurons from patient-derived iPSCs and a knock-in mouse line. G118E mutation partially reduces MeCP2 abundance and its DNA binding, and G118E mice manifest RTT-like symptoms seen in the patient, affirming the pathogenicity of this mutation. Using live-cell and single-molecule imaging, we found that G118E mutation alters MeCP2's chromatin interaction properties in live neurons independently of its effect on protein levels. Here we report the generation and characterization of RTT models of a male hypomorphic variant and reveal new insight into the mechanism by which this pathological mutation affects MeCP2's chromatin dynamics. Our ability to quantify protein dynamics in disease models lays the foundation for harnessing high-resolution single-molecule imaging as the next frontier for developing innovative therapies for RTT and other diseases.


Asunto(s)
Cromatina , Síndrome de Rett , Femenino , Humanos , Masculino , Ratones , Animales , Cromatina/metabolismo , Encéfalo/metabolismo , Proteína 2 de Unión a Metil-CpG/genética , Síndrome de Rett/genética , Mutación , Neuronas/metabolismo
4.
Cell ; 159(2): 295-305, 2014 Oct 09.
Artículo en Inglés | MEDLINE | ID: mdl-25303526

RESUMEN

Human imaging studies have revealed that intranasal administration of the "prosocial" hormone oxytocin (OT) activates the frontal cortex, and this action of OT correlates with enhanced brain function in autism. Here, we report the discovery of a population of somatostatin (Sst)-positive, regular spiking interneurons that express the oxytocin receptor (OxtrINs). Silencing of OxtrINs in the medial prefrontal cortex (mPFC) of female mice resulted in loss of social interest in male mice specifically during the sexually receptive phase of the estrous cycle. This sociosexual deficit was also present in mice in which the Oxtr gene was conditionally deleted from the mPFC and in control mice infused with an Oxtr antagonist. Our data demonstrate a gender-, cell type-, and state-specific role for OT/Oxtr signaling in the mPFC and identify a latent cortical circuit element that may modulate other complex social behaviors in response to OT.


Asunto(s)
Interneuronas/metabolismo , Corteza Prefrontal/metabolismo , Receptores de Oxitocina/metabolismo , Conducta Sexual Animal , Animales , Ciclo Estral , Femenino , Masculino , Ratones , Oxitocina/metabolismo , Corteza Prefrontal/citología , Caracteres Sexuales , Conducta Social
5.
Cell ; 156(3): 522-36, 2014 Jan 30.
Artículo en Inglés | MEDLINE | ID: mdl-24485458

RESUMEN

The extended amygdala has dominated research on the neural circuitry of fear and anxiety, but the septohippocampal axis also plays an important role. The lateral septum (LS) is thought to suppress fear and anxiety through its outputs to the hypothalamus. However, this structure has not yet been dissected using modern tools. The type 2 CRF receptor (Crfr2) marks a subset of LS neurons whose functional connectivity we have investigated using optogenetics. Crfr2(+) cells include GABAergic projection neurons that connect with the anterior hypothalamus. Surprisingly, we find that these LS outputs enhance stress-induced behavioral measures of anxiety. Furthermore, transient activation of Crfr2(+) neurons promotes, while inhibition suppresses, persistent anxious behaviors. LS Crfr2(+) outputs also positively regulate circulating corticosteroid levels. These data identify a subset of LS projection neurons that promote, rather than suppress, stress-induced behavioral and endocrinological dimensions of persistent anxiety states and provide a cellular point of entry to LS circuitry.


Asunto(s)
Ansiedad/fisiopatología , Hipotálamo/metabolismo , Tabique del Cerebro/fisiología , Corticoesteroides/metabolismo , Amígdala del Cerebelo/metabolismo , Animales , Conducta Animal , Femenino , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Transgénicos , Neuronas/fisiología , Receptores de Hormona Liberadora de Corticotropina/metabolismo , Estrés Fisiológico
6.
Cell ; 151(7): 1417-30, 2012 Dec 21.
Artículo en Inglés | MEDLINE | ID: mdl-23260135

RESUMEN

The high level of 5-hydroxymethylcytosine (5hmC) present in neuronal genomes suggests that mechanisms interpreting 5hmC in the CNS may differ from those present in embryonic stem cells. Here, we present quantitative, genome-wide analysis of 5hmC, 5-methylcytosine (5mC), and gene expression in differentiated CNS cell types in vivo. We report that 5hmC is enriched in active genes and that, surprisingly, strong depletion of 5mC is observed over these regions. The contribution of these epigenetic marks to gene expression depends critically on cell type. We identify methyl-CpG-binding protein 2 (MeCP2) as the major 5hmC-binding protein in the brain and demonstrate that MeCP2 binds 5hmC- and 5mC-containing DNA with similar high affinities. The Rett-syndrome-causing mutation R133C preferentially inhibits 5hmC binding. These findings support a model in which 5hmC and MeCP2 constitute a cell-specific epigenetic mechanism for regulation of chromatin structure and gene expression.


Asunto(s)
Cerebelo/metabolismo , Citosina/análogos & derivados , Epigénesis Genética , Proteína 2 de Unión a Metil-CpG/metabolismo , 5-Metilcitosina/análogos & derivados , Animales , Cerebelo/citología , Cromatina/metabolismo , Citosina/metabolismo , Humanos , Ratones , Ratones Noqueados , Neuroglía/metabolismo , Neuronas/metabolismo , Células de Purkinje/metabolismo , Síndrome de Rett/metabolismo
7.
Cell ; 149(5): 1152-63, 2012 May 25.
Artículo en Inglés | MEDLINE | ID: mdl-22632977

RESUMEN

Our understanding of current treatments for depression, and the development of more specific therapies, is limited by the complexity of the circuits controlling mood and the distributed actions of antidepressants. Although the therapeutic efficacy of serotonin-specific reuptake inhibitors (SSRIs) is correlated with increases in cortical activity, the cell types crucial for their action remain unknown. Here we employ bacTRAP translational profiling to show that layer 5 corticostriatal pyramidal cells expressing p11 (S100a10) are strongly and specifically responsive to chronic antidepressant treatment. This response requires p11 and includes the specific induction of Htr4 expression. Cortex-specific deletion of p11 abolishes behavioral responses to SSRIs, but does not lead to increased depression-like behaviors. Our data identify corticostriatal projection neurons as critical for the response to antidepressants, and suggest that the regulation of serotonergic tone in this single cell type plays a pivotal role in antidepressant therapy.


Asunto(s)
Antidepresivos/metabolismo , Depresión/tratamiento farmacológico , Neuronas/citología , Corteza Prefrontal/citología , Inhibidores Selectivos de la Recaptación de Serotonina/metabolismo , Animales , Antidepresivos/farmacología , Modelos Animales de Enfermedad , Humanos , Ratones , Ratones Noqueados , Ratones Transgénicos , Mutación , Corteza Prefrontal/metabolismo , Células Piramidales/metabolismo , Proteínas S100/genética , Proteínas S100/metabolismo , Inhibidores Selectivos de la Recaptación de Serotonina/farmacología
8.
Cell ; 147(7): 1615-27, 2011 Dec 23.
Artículo en Inglés | MEDLINE | ID: mdl-22196735

RESUMEN

Innocuous touch of the skin is detected by distinct populations of neurons, the low-threshold mechanoreceptors (LTMRs), which are classified as Aß-, Aδ-, and C-LTMRs. Here, we report genetic labeling of LTMR subtypes and visualization of their relative patterns of axonal endings in hairy skin and the spinal cord. We found that each of the three major hair follicle types of trunk hairy skin (guard, awl/auchene, and zigzag hairs) is innervated by a unique and invariant combination of LTMRs; thus, each hair follicle type is a functionally distinct mechanosensory end organ. Moreover, the central projections of Aß-, Aδ-, and C-LTMRs that innervate the same or adjacent hair follicles form narrow LTMR columns in the dorsal horn. These findings support a model of mechanosensation in which the activities of Aß-, Aδ-, and C-LTMRs are integrated within dorsal horn LTMR columns and processed into outputs that underlie the perception of myriad touch sensations.


Asunto(s)
Cabello/fisiología , Mecanorreceptores/fisiología , Fenómenos Fisiológicos de la Piel , Piel/inervación , Animales , Axones/fisiología , Ratones , Neuronas/fisiología , Umbral Sensorial , Piel/citología , Médula Espinal/fisiología
9.
Nature ; 586(7829): 407-411, 2020 10.
Artículo en Inglés | MEDLINE | ID: mdl-33029009

RESUMEN

To survive in a dynamic environment, animals need to identify and appropriately respond to stimuli that signal danger1. Survival also depends on suppressing the threat-response during a stimulus that predicts the absence of threat (safety)2-5. An understanding of the biological substrates of emotional memories during a task in which animals learn to flexibly execute defensive responses to a threat-predictive cue and a safety cue is critical for developing treatments for memory disorders such as post-traumatic stress disorder5. The centrolateral amygdala is an important node in the neuronal circuit that mediates defensive responses6-9, and a key brain area for processing and storing threat memories. Here we applied intersectional chemogenetic strategies to inhibitory neurons in the centrolateral amygdala of mice to block cell-type-specific translation programs that are sensitive to depletion of eukaryotic initiation factor 4E (eIF4E) and phosphorylation of eukaryotic initiation factor 2α (p-eIF2α). We show that de novo translation in somatostatin-expressing inhibitory neurons in the centrolateral amygdala is necessary for the long-term storage of conditioned-threat responses, whereas de novo translation in protein kinase Cδ-expressing inhibitory neurons in the centrolateral amygdala is necessary for the inhibition of a conditioned response to a safety cue. Our results provide insight into the role of de novo protein synthesis in distinct inhibitory neuron populations in the centrolateral amygdala during the consolidation of long-term memories.


Asunto(s)
Amígdala del Cerebelo/citología , Amígdala del Cerebelo/fisiología , Emociones , Memoria/fisiología , Inhibición Neural , Neuronas/fisiología , Animales , Condicionamiento Psicológico , Señales (Psicología) , Factor 2 Eucariótico de Iniciación/metabolismo , Factor 4E Eucariótico de Iniciación/metabolismo , Miedo/fisiología , Femenino , Proteínas de Unión al GTP Heterotriméricas/metabolismo , Masculino , Ratones , Biosíntesis de Proteínas , Caperuzas de ARN/genética , Caperuzas de ARN/metabolismo , Transducción de Señal , Somatostatina/metabolismo
10.
Nature ; 587(7833): 258-263, 2020 11.
Artículo en Inglés | MEDLINE | ID: mdl-33116307

RESUMEN

The anterolateral pathway consists of ascending spinal tracts that convey pain, temperature and touch information from the spinal cord to the brain1-4. Projection neurons of the anterolateral pathway are attractive therapeutic targets for pain treatment because nociceptive signals emanating from the periphery are channelled through these spinal projection neurons en route to the brain. However, the organizational logic of the anterolateral pathway remains poorly understood. Here we show that two populations of projection neurons that express the structurally related G-protein-coupled receptors (GPCRs) TACR1 and GPR83 form parallel ascending circuit modules that cooperate to convey thermal, tactile and noxious cutaneous signals from the spinal cord to the lateral parabrachial nucleus of the pons. Within this nucleus, axons of spinoparabrachial (SPB) neurons that express Tacr1 or Gpr83 innervate distinct sets of subnuclei, and strong optogenetic stimulation of the axon terminals induces distinct escape behaviours and autonomic responses. Moreover, SPB neurons that  express Gpr83 are highly sensitive to cutaneous mechanical stimuli and receive strong synaptic inputs from both high- and low-threshold primary mechanosensory neurons. Notably, the valence associated with activation of SPB neurons that express Gpr83 can be either positive or negative, depending on stimulus intensity. These findings reveal anatomically, physiologically and functionally distinct subdivisions of the SPB tract that underlie affective aspects of touch and pain.


Asunto(s)
Vías Nerviosas , Dolor/fisiopatología , Médula Espinal/citología , Médula Espinal/fisiología , Tacto/fisiología , Animales , Axones/metabolismo , Femenino , Masculino , Mecanotransducción Celular , Ratones , Filosofía , Receptores Acoplados a Proteínas G/genética , Células Receptoras Sensoriales/metabolismo , Piel/inervación , Sinapsis/metabolismo
11.
Proc Natl Acad Sci U S A ; 119(49): e2211454119, 2022 12 06.
Artículo en Inglés | MEDLINE | ID: mdl-36442105

RESUMEN

Neuromodulatory substances can be released from distal afferents for communication between brain structures or produced locally to modulate neighboring circuit elements. Corticotropin-releasing hormone (CRH) from long-range neurons in the hypothalamus projecting to the medial prefrontal cortex (mPFC) has been shown to induce anxiety-like behaviors. However, the role of CRH produced in the mPFC has not been investigated. Here we demonstrate that a specific class of mPFC interneurons that express CRH (CrhINs) releases CRH upon high-frequency stimulation to enhance excitability of layer 2/3 pyramidal cells (L2/3 PCs) expressing the CRH receptors. When stimulated at low frequency, CrhINs release GABA resulting in the inhibition of oxytocin receptor-expressing interneurons (OxtrINs) and L2/3 PCs. Conditional deletion of CRH in mPFC CrhINs and chemogenetic activation of CrhINs have opposite effects on novelty exploration in male but not in female mice, and do not affect anxiety-related behaviors in either males or females. Our data reveal that CRH produced by local interneurons in the mPFC is required for sex-specific novelty exploration and suggest that our understanding of complex behaviors may require knowledge of local and remote neuromodulatory action.


Asunto(s)
Hormona Liberadora de Corticotropina , Corteza Prefrontal , Femenino , Masculino , Animales , Ratones , Hormona Liberadora de Corticotropina/genética , Receptores de Hormona Liberadora de Corticotropina , Células Piramidales , Interneuronas
12.
Proc Natl Acad Sci U S A ; 119(21): e2122544119, 2022 05 24.
Artículo en Inglés | MEDLINE | ID: mdl-35588456

RESUMEN

Environmental perturbations during the first years of life are a major factor in psychiatric diseases. Phencyclidine (PCP), a drug of abuse, has psychomimetic effects, and neonatal subchronic administration of PCP in rodents leads to long-term behavioral changes relevant for schizophrenia. The cerebellum is increasingly recognized for its role in diverse cognitive functions. However, little is known about potential cerebellar changes in models of schizophrenia. Here, we analyzed the characteristics of the cerebellum in the neonatal subchronic PCP model. We found that, while the global cerebellar cytoarchitecture and Purkinje cell spontaneous spiking properties are unchanged, climbing fiber/Purkinje cell synaptic connectivity is increased in juvenile mice. Neonatal subchronic administration of PCP is accompanied by increased cFos expression, a marker of neuronal activity, and transient modification of the neuronal surfaceome in the cerebellum. The largest change observed is the overexpression of Ctgf, a gene previously suggested as a biomarker for schizophrenia. This neonatal increase in Ctgf can be reproduced by increasing neuronal activity in the cerebellum during the second postnatal week using chemogenetics. However, it does not lead to increased climbing fiber/Purkinje cell connectivity in juvenile mice, showing the complexity of PCP action. Overall, our study shows that administration of the drug of abuse PCP during the developmental period of intense cerebellar synaptogenesis and circuit remodeling has long-term and specific effects on Purkinje cell connectivity and warrants the search for this type of synaptic changes in psychiatric diseases.


Asunto(s)
Alucinógenos , Fenciclidina , Células de Purkinje , Esquizofrenia , Animales , Factor de Crecimiento del Tejido Conjuntivo/genética , Factor de Crecimiento del Tejido Conjuntivo/metabolismo , Modelos Animales de Enfermedad , Alucinógenos/administración & dosificación , Alucinógenos/efectos adversos , Ratones , Neuronas/efectos de los fármacos , Neuronas/metabolismo , Fenciclidina/administración & dosificación , Fenciclidina/efectos adversos , Proteínas Proto-Oncogénicas c-fos/metabolismo , Células de Purkinje/efectos de los fármacos , Células de Purkinje/fisiología , Células de Purkinje/ultraestructura , Receptores de Fenciclidina/agonistas , Esquizofrenia/inducido químicamente , Esquizofrenia/patología , Sinapsis/efectos de los fármacos , Sinapsis/ultraestructura
13.
Mol Psychiatry ; 27(4): 2068-2079, 2022 04.
Artículo en Inglés | MEDLINE | ID: mdl-35177825

RESUMEN

Forebrain dopamine-sensitive (dopaminoceptive) neurons play a key role in movement, action selection, motivation, and working memory. Their activity is altered in Parkinson's disease, addiction, schizophrenia, and other conditions, and drugs that stimulate or antagonize dopamine receptors have major therapeutic applications. Yet, similarities and differences between the various neuronal populations sensitive to dopamine have not been systematically explored. To characterize them, we compared translating mRNAs in the dorsal striatum and nucleus accumbens neurons expressing D1 or D2 dopamine receptor and prefrontal cortex neurons expressing D1 receptor. We identified genome-wide cortico-striatal, striatal D1/D2 and dorso/ventral differences in the translating mRNA and isoform landscapes, which characterize dopaminoceptive neuronal populations. Expression patterns and network analyses identified novel transcription factors with presumptive roles in these differences. Prostaglandin E2 (PGE2) was a candidate upstream regulator in the dorsal striatum. We pharmacologically explored this hypothesis and showed that misoprostol, a PGE2 receptor agonist, decreased the excitability of D2 striatal projection neurons in slices, and diminished their activity in vivo during novel environment exploration. We found that misoprostol also modulates mouse behavior including by facilitating reversal learning. Our study provides powerful resources for characterizing dopamine target neurons, new information about striatal gene expression patterns and regulation. It also reveals the unforeseen role of PGE2 in the striatum as a potential neuromodulator and an attractive therapeutic target.


Asunto(s)
Dinoprostona , Misoprostol , Animales , Cuerpo Estriado/metabolismo , Dinoprostona/metabolismo , Dinoprostona/farmacología , Dopamina/metabolismo , Neuronas Dopaminérgicas/metabolismo , Exones , Expresión Génica , Ratones , Misoprostol/metabolismo , Misoprostol/farmacología , ARN Mensajero/metabolismo , Receptores de Dopamina D1/metabolismo
14.
Cell ; 135(4): 738-48, 2008 Nov 14.
Artículo en Inglés | MEDLINE | ID: mdl-19013281

RESUMEN

The cellular heterogeneity of the brain confounds efforts to elucidate the biological properties of distinct neuronal populations. Using bacterial artificial chromosome (BAC) transgenic mice that express EGFP-tagged ribosomal protein L10a in defined cell populations, we have developed a methodology for affinity purification of polysomal mRNAs from genetically defined cell populations in the brain. The utility of this approach is illustrated by the comparative analysis of four types of neurons, revealing hundreds of genes that distinguish these four cell populations. We find that even two morphologically indistinguishable, intermixed subclasses of medium spiny neurons display vastly different translational profiles and present examples of the physiological significance of such differences. This genetically targeted translating ribosome affinity purification (TRAP) methodology is a generalizable method useful for the identification of molecular changes in any genetically defined cell type in response to genetic alterations, disease, or pharmacological perturbations.


Asunto(s)
Encéfalo/metabolismo , Técnicas Genéticas , Biosíntesis de Proteínas , Animales , Sistema Nervioso Central/metabolismo , Cromosomas Artificiales Bacterianos/metabolismo , Cocaína/farmacología , Inhibidores de Captación de Dopamina/farmacología , Proteínas Fluorescentes Verdes/metabolismo , Inmunohistoquímica/métodos , Ratones , Ratones Transgénicos , Modelos Biológicos , Neuronas/metabolismo , Ribosomas/metabolismo
15.
Cell ; 135(4): 749-62, 2008 Nov 14.
Artículo en Inglés | MEDLINE | ID: mdl-19013282

RESUMEN

Comparative analysis can provide important insights into complex biological systems. As demonstrated in the accompanying paper, translating ribosome affinity purification (TRAP) permits comprehensive studies of translated mRNAs in genetically defined cell populations after physiological perturbations. To establish the generality of this approach, we present translational profiles for 24 CNS cell populations and identify known cell-specific and enriched transcripts for each population. We report thousands of cell-specific mRNAs that were not detected in whole-tissue microarray studies and provide examples that demonstrate the benefits deriving from comparative analysis. To provide a foundation for further biological and in silico studies, we provide a resource of 16 transgenic mouse lines, their corresponding anatomic characterization, and translational profiles for cell types from a variety of central nervous system structures. This resource will enable a wide spectrum of molecular and mechanistic studies of both well-known and previously uncharacterized neural cell populations.


Asunto(s)
Encéfalo/metabolismo , Técnicas Genéticas , Biosíntesis de Proteínas , Animales , Sistema Nervioso Central/metabolismo , Cromosomas Artificiales Bacterianos/metabolismo , Proteínas Fluorescentes Verdes/metabolismo , Inmunohistoquímica/métodos , Ratones , Ratones Transgénicos , Modelos Biológicos , Neuronas/metabolismo , Análisis de Secuencia por Matrices de Oligonucleótidos , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Ribosomas/metabolismo
16.
Proc Natl Acad Sci U S A ; 117(10): 5502-5509, 2020 03 10.
Artículo en Inglés | MEDLINE | ID: mdl-32098843

RESUMEN

The habenula, an ancient small brain area in the epithalamus, densely expresses nicotinic acetylcholine receptors and is critical for nicotine intake and aversion. As such, identification of strategies to manipulate habenular activity may yield approaches to treat nicotine addiction. Here we show that GPR151, an orphan G-protein-coupled receptor (GPCR) highly enriched in the habenula of humans and rodents, is expressed at presynaptic membranes and synaptic vesicles and associates with synaptic components controlling vesicle release and ion transport. Deletion of Gpr151 inhibits evoked neurotransmission but enhances spontaneous miniature synaptic currents and eliminates short-term plasticity induced by nicotine. We find that GPR151 couples to the G-alpha inhibitory protein Gαo1 to reduce cyclic adenosine monophosphate (cAMP) levels in mice and in GPR151-expressing cell lines that are amenable to ligand screens. Gpr151- knockout (KO) mice show diminished behavioral responses to nicotine and self-administer greater quantities of the drug, phenotypes rescued by viral reexpression of Gpr151 in the habenula. These data identify GPR151 as a critical modulator of habenular function that controls nicotine addiction vulnerability.


Asunto(s)
Habénula/fisiología , Plasticidad Neuronal/fisiología , Nicotina/metabolismo , Agonistas Nicotínicos/metabolismo , Receptores Acoplados a Proteínas G/fisiología , Trastornos Relacionados con Sustancias/metabolismo , Animales , Células CHO , Cricetulus , Subunidades alfa de la Proteína de Unión al GTP Gi-Go/metabolismo , Habénula/metabolismo , Humanos , Ratones Noqueados , Plasticidad Neuronal/efectos de los fármacos , Plasticidad Neuronal/genética , Nicotina/administración & dosificación , Agonistas Nicotínicos/administración & dosificación , Receptores Acoplados a Proteínas G/genética , Transmisión Sináptica/genética , Transmisión Sináptica/fisiología
17.
Mol Psychiatry ; 26(11): 7029-7046, 2021 11.
Artículo en Inglés | MEDLINE | ID: mdl-34099874

RESUMEN

The subthalamic nucleus (STN) is a component of the basal ganglia and plays a key role to control movement and limbic-associative functions. STN modulation with deep brain stimulation (DBS) improves the symptoms of Parkinson's disease (PD) and obsessive-compulsive disorder (OCD) patients. However, DBS does not allow for cell-type-specific modulation of the STN. While extensive work has focused on elucidating STN functionality, the understanding of the role of specific cell types is limited. Here, we first performed an anatomical characterization of molecular markers for specific STN neurons. These studies revealed that most STN neurons express Pitx2, and that different overlapping subsets express Gabrr3, Ndnf, or Nos1. Next, we used optogenetics to define their roles in regulating locomotor and limbic functions in mice. Specifically, we showed that optogenetic photoactivation of STN neurons in Pitx2-Cre mice or of the Gabrr3-expressing subpopulation induces locomotor changes, and improves locomotion in a PD mouse model. In addition, photoactivation of Pitx2 and Gabrr3 cells induced repetitive grooming, a phenotype associated with OCD. Repeated stimulation prompted a persistent increase in grooming that could be reversed by fluoxetine treatment, a first-line drug therapy for OCD. Conversely, repeated inhibition of STNGabrr3 neurons suppressed grooming in Sapap3 KO mice, a model for OCD. Finally, circuit and functional mapping of STNGabrr3 neurons showed that these effects are mediated via projections to the globus pallidus/entopeduncular nucleus and substantia nigra reticulata. Altogether, these data identify Gabrr3 neurons as a key population in mediating the beneficial effects of STN modulation thus providing potential cellular targets for PD and OCD drug discovery.


Asunto(s)
Trastorno Obsesivo Compulsivo , Enfermedad de Parkinson , Núcleo Subtalámico , Animales , Ratones , Proteínas del Tejido Nervioso , Neuronas/fisiología , Trastorno Obsesivo Compulsivo/terapia , Enfermedad de Parkinson/terapia
18.
Mol Psychiatry ; 26(6): 2334-2349, 2021 06.
Artículo en Inglés | MEDLINE | ID: mdl-33441982

RESUMEN

Serotonin receptor 4 (5-HT4R) plays an important role in regulating mood, anxiety, and cognition, and drugs that activate this receptor have fast-acting antidepressant (AD)-like effects in preclinical models. However, 5-HT4R is widely expressed throughout the central nervous system (CNS) and periphery, making it difficult to pinpoint the cell types and circuits underlying its effects. Therefore, we generated a Cre-dependent 5-HT4R knockout mouse line to dissect the function of 5-HT4R in specific brain regions and cell types. We show that the loss of functional 5-HT4R specifically from excitatory neurons of hippocampus led to robust AD-like behavioral responses and an elevation in baseline anxiety. 5-HT4R was necessary to maintain the proper excitability of dentate gyrus (DG) granule cells and cell type-specific molecular profiling revealed a dysregulation of genes necessary for normal neural function and plasticity in cells lacking 5-HT4R. These adaptations were accompanied by an increase in the number of immature neurons in ventral, but not dorsal, dentate gyrus, indicating a broad impact of 5-HT4R loss on the local cellular environment. This study is the first to use conditional genetic targeting to demonstrate a direct role for hippocampal 5-HT4R signaling in modulating mood and anxiety. Our findings also underscore the need for cell type-based approaches to elucidate the complex action of neuromodulatory systems on distinct neural circuits.


Asunto(s)
Ansiedad , Hipocampo , Animales , Giro Dentado/metabolismo , Hipocampo/metabolismo , Ratones , Neuronas/metabolismo , Receptores de Serotonina , Receptores de Serotonina 5-HT4/genética , Receptores de Serotonina 5-HT4/metabolismo
19.
Genes Dev ; 27(5): 565-78, 2013 Mar 01.
Artículo en Inglés | MEDLINE | ID: mdl-23431030

RESUMEN

Hypocretin (orexin; Hcrt)-containing neurons of the hypothalamus are essential for the normal regulation of sleep and wake behaviors and have been implicated in feeding, anxiety, depression, and reward. The absence of these neurons causes narcolepsy in humans and model organisms. However, little is known about the molecular phenotype of these cells; previous attempts at comprehensive profiling had only limited sensitivity or were inaccurate. We generated a Hcrt translating ribosome affinity purification (bacTRAP) line for comprehensive translational profiling of all ribosome-bound transcripts in these neurons in vivo. From this profile, we identified >6000 transcripts detectably expressed above background and 188 transcripts that are highly enriched in these neurons, including all known markers of the cells. Blinded analysis of in situ hybridization databases suggests that ~60% of these are expressed in a Hcrt marker-like pattern. Fifteen of these were confirmed with double labeling and microscopy, including the transcription factor Lhx9. Ablation of this gene results in a >30% loss specifically of Hcrt neurons, without a general disruption of hypothalamic development. Polysomnography and activity monitoring revealed a profound hypersomnolence in these mice. These data provide an in-depth and accurate profile of Hcrt neuron gene expression and suggest that Lhx9 may be important for specification or survival of a subset of these cells.


Asunto(s)
Perfilación de la Expresión Génica , Regulación de la Expresión Génica , Péptidos y Proteínas de Señalización Intracelular , Neuronas/metabolismo , Neuropéptidos , Sueño/fisiología , Animales , Femenino , Hipotálamo/citología , Hipotálamo/metabolismo , Proteínas con Homeodominio LIM/genética , Proteínas con Homeodominio LIM/metabolismo , Masculino , Ratones , Ratones Endogámicos C57BL , Orexinas , Regiones Promotoras Genéticas/genética , Análisis por Matrices de Proteínas , Sueño/genética , Factores de Transcripción/genética , Factores de Transcripción/metabolismo
20.
Proc Natl Acad Sci U S A ; 114(37): E7812-E7821, 2017 09 12.
Artículo en Inglés | MEDLINE | ID: mdl-28847947

RESUMEN

5-hydroxymethylcytosine (5hmC) occurs at maximal levels in postmitotic neurons, where its accumulation is cell-specific and correlated with gene expression. Here we demonstrate that the distribution of 5hmC in CG and non-CG dinucleotides is distinct and that it reflects the binding specificity and genome occupancy of methylcytosine binding protein 2 (MeCP2). In expressed gene bodies, accumulation of 5hmCG acts in opposition to 5mCG, resulting in "functional" demethylation and diminished MeCP2 binding, thus facilitating transcription. Non-CG hydroxymethylation occurs predominantly in CA dinucleotides (5hmCA) and it accumulates in regions flanking active enhancers. In these domains, oxidation of 5mCA to 5hmCA does not alter MeCP2 binding or expression of adjacent genes. We conclude that the role of 5-hydroxymethylcytosine in postmitotic neurons is to functionally demethylate expressed gene bodies while retaining the role of MeCP2 in chromatin organization.


Asunto(s)
5-Metilcitosina/análogos & derivados , Metilación de ADN , Neuronas/metabolismo , 5-Metilcitosina/metabolismo , Animales , Citosina/metabolismo , Desmetilación , Epigénesis Genética , Expresión Génica , Proteína 2 de Unión a Metil-CpG/genética , Proteína 2 de Unión a Metil-CpG/metabolismo , Ratones , Mitosis/fisiología , Regiones Promotoras Genéticas
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