1.
Mol Cell Biol
; 11(6): 3374-8, 1991 Jun.
Artículo
en Inglés
| MEDLINE
| ID: mdl-1645450
RESUMEN
The hygromycin phosphotransferase gene was fused in-frame with the herpes simplex virus type 1 thymidine kinase gene. The resulting fusion gene (termed HyTK) confers hygromycin B resistance for dominant positive selection and ganciclovir sensitivity for negative selection and provides a means by which these selectable phenotypes may be expressed and regulated as a single genetic entity.