Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Más filtros

Banco de datos
Tipo del documento
País de afiliación
Intervalo de año de publicación
1.
J Chromatogr A ; 1218(27): 4160-6, 2011 Jul 08.
Artículo en Inglés | MEDLINE | ID: mdl-21129749

RESUMEN

Nanoparticles are not typically ready-to-use for in vitro cell culture assays. Prior to their use in assays, powder samples containing nanoparticles must be dispersed, de-agglomerated, fractionated by size, and characterized with respect to size and size distribution. For this purpose we report exemplarily on polyphosphate-stabilized iron oxide nanoparticles in aqueous suspension. Fractionation and online particle size analysis was performed in a time-saving procedure lasting 50 min by combining asymmetrical flow field-flow fractionation (A4F) and small-angle X-ray scattering (SAXS). Narrowly distributed nanoparticle fractions with radii of gyration (R(g)) from 7 to 21 nm were obtained from polydisperse samples. The A4F-SAXS combination is introduced for the preparation of well-characterized sample fractions originating from a highly polydisperse system as typically found in engineered nanoparticles. A4F-SAXS processed particles are ready-to-use for toxicological studies. The results of preliminary tests of the effects of fractionated iron oxide nanoparticles with a R(g) of 15 nm on a human colon model cell line are reported.


Asunto(s)
Compuestos Férricos/química , Fraccionamiento de Campo-Flujo/métodos , Nanopartículas del Metal/química , Dispersión del Ángulo Pequeño , Difracción de Rayos X/métodos , Células CACO-2 , Proliferación Celular/efectos de los fármacos , Compuestos Férricos/toxicidad , Humanos , Nanopartículas del Metal/toxicidad , Nanopartículas del Metal/ultraestructura , Microscopía Electrónica de Transmisión , Modelos Biológicos , Tamaño de la Partícula , Espectrofotometría Ultravioleta
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA