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1.
Graefes Arch Clin Exp Ophthalmol ; 262(2): 469-476, 2024 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-37864637

RESUMEN

PURPOSE: To assess epiretinal membrane (ERM) formation, severity, and the associated risk factors after scleral buckling using en face optical coherence tomography (OCT) images. METHODS: Medical records of 61 consecutive patients (66 eyes) with rhegmatogenous retinal detachment who underwent scleral buckling were retrospectively reviewed. Posterior vitreous detachment (PVD) was determined based on B-scan OCT images. En face OCT images were used to visualize the ERM and retinal folds. ERM formation was identified by comparing en face images pre- and post-surgery. The maximum depth of the retinal folds (MDRF) was measured using en face imaging to objectively assess traction strength. RESULTS: ERM formation occurred in 15 (22.7%) eyes at the final visit; the foveal pit was preserved in all cases. Parafoveal retinal folds were present in 5 (7.6%) eyes, with a mean MDRF of 21.8 ± 12.6 µm. No significant difference was observed in best-corrected visual acuity (logarithm of the minimal angle of resolution) between the ERM formation (-0.019 ± 0.128) and non-ERM formation (-0.001 ± 0.213) groups at the final visit (P = 0.593; Mann-Whitney U test). Multivariate logistic regression analysis revealed that older age and the presence of PVD were significant risk factors for ERM formation (odds ratio 1.07, 95% confidence interval 1.01-1.14, P = 0.032; odds ratio 5.26, 95% confidence interval 1.06-26.10, P = 0.042; respectively). CONCLUSION: ERM occurred in 22.7% of cases but was mild and did not affect visual acuity. Older age and the presence of PVD are risk factors for ERM formation.


Asunto(s)
Membrana Epirretinal , Desprendimiento de Retina , Desprendimiento del Vítreo , Humanos , Curvatura de la Esclerótica , Desprendimiento de Retina/diagnóstico , Desprendimiento de Retina/etiología , Desprendimiento de Retina/cirugía , Membrana Epirretinal/diagnóstico , Membrana Epirretinal/etiología , Membrana Epirretinal/cirugía , Tomografía de Coherencia Óptica/métodos , Estudios Retrospectivos , Desprendimiento del Vítreo/cirugía , Vitrectomía/efectos adversos
2.
Graefes Arch Clin Exp Ophthalmol ; 262(10): 3099-3107, 2024 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-38625447

RESUMEN

PURPOSE: To investigate the influence of submacular hemorrhage (SMH) at baseline on long-term visual outcomes of patients with typical age-related macular degeneration (tAMD) and polypoidal choroidal vasculopathy (PCV) treated with intravitreal aflibercept (IVA). METHODS: In this retrospective study, eyes of treatment-naïve patients with tAMD and PCV who initiated IVA under a treat-and-extend regimen and were followed up for ≥ 5 years were classified into the tAMD-SMH ( +), tAMD-SMH (-), PCV-SMH ( +), and PCV-SMH (-) groups based on the presence of SMH at baseline. Best-corrected visual acuity (BCVA) changes and macular fibrosis and atrophy incidences were assessed. RESULTS: This study included 127 eyes (127 patients), including 51 with tAMD and 76 with PCV; 18 eyes had SMH at baseline. In the tAMD-SMH ( +) group (n = 6), the mean logMAR BCVA significantly deteriorated from 0.59 ± 0.45 at baseline to 0.88 ± 0.47 at the final visit (P = 0.024). No significant BCVA changes were observed in the tAMD-SMH (-) (n = 45), PCV-SMH ( +) (n = 12), or PCV-SMH (-) (n = 64) groups (all P > 0.05). The tAMD-SMH ( +) group showed a significantly higher incidence of macular fibrosis at the final visit than did the tAMD-SMH (-) group (P = 0.042). There was no influence of baseline SMH on the macular fibrosis incidence in eyes with PCV and the macular atrophy incidence in eyes with tAMD and PCV. CONCLUSION: The presence of SMH at baseline resulted in poorer long-term visual acuity in eyes with tAMD, even with aflibercept treatment. However, no such influence was observed in eyes with PCV.


Asunto(s)
Angiografía con Fluoresceína , Fondo de Ojo , Inyecciones Intravítreas , Pólipos , Receptores de Factores de Crecimiento Endotelial Vascular , Proteínas Recombinantes de Fusión , Tomografía de Coherencia Óptica , Agudeza Visual , Degeneración Macular Húmeda , Humanos , Receptores de Factores de Crecimiento Endotelial Vascular/administración & dosificación , Proteínas Recombinantes de Fusión/administración & dosificación , Estudios Retrospectivos , Masculino , Femenino , Anciano , Tomografía de Coherencia Óptica/métodos , Angiografía con Fluoresceína/métodos , Estudios de Seguimiento , Degeneración Macular Húmeda/tratamiento farmacológico , Degeneración Macular Húmeda/diagnóstico , Degeneración Macular Húmeda/fisiopatología , Pólipos/diagnóstico , Pólipos/tratamiento farmacológico , Pólipos/fisiopatología , Resultado del Tratamiento , Factores de Tiempo , Hemorragia Retiniana/diagnóstico , Hemorragia Retiniana/etiología , Hemorragia Retiniana/fisiopatología , Hemorragia Retiniana/tratamiento farmacológico , Coroides/irrigación sanguínea , Neovascularización Coroidal/tratamiento farmacológico , Neovascularización Coroidal/diagnóstico , Neovascularización Coroidal/fisiopatología , Neovascularización Coroidal/etiología , Inhibidores de la Angiogénesis/administración & dosificación , Inhibidores de la Angiogénesis/uso terapéutico , Mácula Lútea/patología , Factor A de Crecimiento Endotelial Vascular/antagonistas & inhibidores , Anciano de 80 o más Años , Persona de Mediana Edad , Vasculopatía Coroidea Polipoidea
3.
Retina ; 44(11): 1992-2000, 2024 11 01.
Artículo en Inglés | MEDLINE | ID: mdl-39024655

RESUMEN

PURPOSE: To analyze the pathophysiology of epiretinal membrane foveoschisis (FS) by evaluating the longitudinal changes in visual function and several optical coherence tomography parameters. METHODS: The medical records of 33 consecutive patients (35 eyes) with untreated epiretinal membrane foveoschisis were retrospectively reviewed. Best-corrected visual acuity, M-CHARTS score, and optical coherence tomography parameters including epiretinal membrane area, maximum depth of retinal folds, FS area, and FS circularity were evaluated. RESULTS: A wide range of FS area changes was observed at the final follow-up visit (59.68%-240.45% of the baseline FS area). In the FS enlargement group, best-corrected visual acuity and mean M-CHARTS scores significantly worsened and maximum depth of retinal folds significantly increased over time, whereas in the FS non-enlargement group, no significant change was observed in the best-corrected visual acuity, mean M-CHARTS scores, or maximum depth of retinal folds during the follow-up period. Multivariate logistic regression analyses revealed that maximum depth of retinal folds (odds ratio: 1.05, 95% confidence interval: 1.00-1.10, P = 0.048) and FS circularity (odds ratio: 0.91, 95% confidence interval: 0.83-1.00, P = 0.043) were significantly associated with FS enlargement. CONCLUSION: Epiretinal membrane foveoschisis encompasses diverse pathophysiologies. Since visual functions do not worsen in some cases, monitoring the changes in visual functions and retinal morphology over time is recommended to determine surgical indications.


Asunto(s)
Membrana Epirretinal , Retinosquisis , Tomografía de Coherencia Óptica , Agudeza Visual , Humanos , Membrana Epirretinal/fisiopatología , Membrana Epirretinal/diagnóstico , Tomografía de Coherencia Óptica/métodos , Agudeza Visual/fisiología , Masculino , Estudios Retrospectivos , Femenino , Anciano , Retinosquisis/fisiopatología , Retinosquisis/diagnóstico , Persona de Mediana Edad , Estudios de Seguimiento , Fóvea Central/fisiopatología , Fóvea Central/patología , Fóvea Central/diagnóstico por imagen , Anciano de 80 o más Años
4.
Retina ; 44(10): 1785-1792, 2024 10 01.
Artículo en Inglés | MEDLINE | ID: mdl-39287541

RESUMEN

PURPOSE: To investigate the characteristics of mixed pathophysiologies in lamellar macular holes (LMHs) and related diseases using multimodal optical coherence tomography. METHODS: Overall, 126 eyes diagnosed with LMH, epiretinal membrane foveoschisis, or macular pseudohole using the horizontal B-scan image according to the definition proposed by Hubschman et al in 2020 were analyzed using multimodal optical coherence tomography imaging including horizontal and vertical 5-line B-scan, radial scan, and macular three-dimensional volume scan images. If at least two diagnostic criteria for LMH, epiretinal membrane foveoschisis, or macular pseudohole were satisfied in these scans, the patient was diagnosed as having a "mixed type." Retinal traction force was quantitatively evaluated by measuring the maximum depth of the retinal folds using en-face images. RESULTS: Mixed types constituted 34.1% of the cases. The LMH-related mixed group demonstrated intermediate characteristics between the epiretinal membrane foveoschisis/macular pseudohole and true LMH groups in terms of retinal traction and LMH-specific features and had a significant positive correlation between the maximum depth of the retinal folds and mean M-CHARTS scores (P = 0.034). CONCLUSION: A thorough optical coherence tomography analysis is necessary to accurately diagnose LMH and related diseases. A significant positive correlation was observed between the maximum depth of the retinal folds and the degree of metamorphopsia in the LMH-related mixed group.


Asunto(s)
Membrana Epirretinal , Perforaciones de la Retina , Tomografía de Coherencia Óptica , Agudeza Visual , Humanos , Tomografía de Coherencia Óptica/métodos , Perforaciones de la Retina/diagnóstico , Perforaciones de la Retina/fisiopatología , Perforaciones de la Retina/diagnóstico por imagen , Femenino , Masculino , Anciano , Membrana Epirretinal/diagnóstico , Membrana Epirretinal/fisiopatología , Membrana Epirretinal/diagnóstico por imagen , Agudeza Visual/fisiología , Estudios Retrospectivos , Persona de Mediana Edad , Imagen Multimodal , Retinosquisis/diagnóstico , Retinosquisis/fisiopatología , Retinosquisis/diagnóstico por imagen , Mácula Lútea/diagnóstico por imagen , Mácula Lútea/patología , Anciano de 80 o más Años
5.
Acta Med Okayama ; 78(3): 295-300, 2024 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-38902219

RESUMEN

A Japanese woman in her 70s was referred to our hospital for the evaluation and treatment of high intraocular pressure (IOP) in her right eye. She had undergone bilateral cataract surgeries and the insertion of hydrophilic acrylic intraocular lenses (IOLs). We performed trabeculotomy and trabeculectomy to lower her right IOP; thereafter, a circular opacity was observed on the right eye's IOL surface. We removed the right IOL because that eye's vision had decreased due to IOL opacification. The analysis of the removed IOL revealed that the main opacity component was calcium phosphate. This is the first post-glaucoma-surgery IOL calcification case report.


Asunto(s)
Calcinosis , Glaucoma , Lentes Intraoculares , Humanos , Femenino , Lentes Intraoculares/efectos adversos , Calcinosis/etiología , Anciano , Glaucoma/cirugía , Glaucoma/etiología , Implantación de Lentes Intraoculares/efectos adversos , Complicaciones Posoperatorias/etiología
6.
Retina ; 43(4): 585-593, 2023 04 01.
Artículo en Inglés | MEDLINE | ID: mdl-36735920

RESUMEN

PURPOSE: To compare the effects of macular intraretinal hemorrhage (IRH) and macular hole (MH) on best-corrected visual acuity (BCVA) after displacement of submacular hemorrhage (SMH) due to retinal arterial macroaneurysm (RAM) rupture. METHODS: This multicenter retrospective study included 48 eyes with SMH due to RAM rupture. Cases underwent vitrectomy to displace SMH and were followed up for 6 months. We classified cases according to the presence of IRH and MH and compared the postoperative BCVA among the groups. RESULTS: We classified the eyes into IRH(+)MH(+) group (10 eyes), IRH(+)MH(-) group (23 eyes), and IRH(-)MH(-) group (15 eyes). The postoperative BCVA was significantly worse in the IRH(+)MH(+) and IRH(+)MH(-) groups than in the IRH(-)MH(-) group (0.91 ± 0.41 in logarithm of the minimal angle of resolution units, Snellen equivalent 20/163, 0.87 ± 0.45, 20/148, and 0.18 ± 0.21, 20/30, respectively; P < 0.001). The postoperative central retinal thickness was significantly lower in the IRH(+) group (IRH(+)MH(+) and IRH(+)MH(-) groups combined) than in the IRH(-) group (IRH(-)MH(-) group) (121.4 ± 70.1 µ m and 174.3 ± 32.9 µ m, respectively, P = 0.008). The postoperative external limiting membrane and ellipsoid zone continuities were significantly discontinuous in the IRH(+) group ( P < 0.001, P = 0.001, respectively). The multiple linear regression analysis showed that both IRH(+)MH(+) and IRH(+)MH(-) were associated with the postoperative BCVA (regression coefficient, 0.799 and 0.711, respectively; P < 0.001 for both). CONCLUSION: Both IRH and MH were poor prognostic indicators in cases with SMH due to RAM rupture.


Asunto(s)
Macroaneurisma Arterial de Retina , Perforaciones de la Retina , Humanos , Perforaciones de la Retina/diagnóstico , Perforaciones de la Retina/etiología , Perforaciones de la Retina/cirugía , Estudios Retrospectivos , Macroaneurisma Arterial de Retina/complicaciones , Macroaneurisma Arterial de Retina/diagnóstico , Hemorragia Retiniana/diagnóstico , Hemorragia Retiniana/etiología , Hemorragia Retiniana/cirugía , Pronóstico , Vitrectomía , Tomografía de Coherencia Óptica
7.
PLoS Genet ; 16(3): e1008543, 2020 03.
Artículo en Inglés | MEDLINE | ID: mdl-32134927

RESUMEN

Following fertilization of a mature oocyte, the formation of a diploid zygote involves a series of coordinated cellular events that ends with the first embryonic mitosis. In animals, this complex developmental transition is almost entirely controlled by maternal gene products. How such a crucial transcriptional program is established during oogenesis remains poorly understood. Here, we have performed an shRNA-based genetic screen in Drosophila to identify genes required to form a diploid zygote. We found that the Lid/KDM5 histone demethylase and its partner, the Sin3A-HDAC1 deacetylase complex, are necessary for sperm nuclear decompaction and karyogamy. Surprisingly, transcriptomic analyses revealed that these histone modifiers are required for the massive transcriptional activation of deadhead (dhd), which encodes a maternal thioredoxin involved in sperm chromatin remodeling. Unexpectedly, while lid knock-down tends to slightly favor the accumulation of its target, H3K4me3, on the genome, this mark was lost at the dhd locus. We propose that Lid/KDM5 and Sin3A cooperate to establish a local chromatin environment facilitating the unusually high expression of dhd, a key effector of the oocyte-to-zygote transition.


Asunto(s)
Proteínas de Drosophila/genética , Histona Demetilasas/genética , Oocitos/fisiología , Cigoto/fisiología , Animales , Núcleo Celular/genética , Cromatina/genética , Ensamble y Desensamble de Cromatina/genética , Drosophila/genética , Femenino , Regulación del Desarrollo de la Expresión Génica/genética , Histonas/genética , Masculino , Oogénesis/genética , Espermatozoides/fisiología , Transcripción Genética/genética
8.
Acta Med Okayama ; 77(4): 433-437, 2023 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-37635145

RESUMEN

A 23-year-old woman presented with a 1-month history of visual abnormalities in her right eye. A visual field test revealed temporal abnormalities in the right eye. Optical coherence tomography revealed an indistinct ellipsoid zone (EZ) on the B-scan image and hyporeflective areas in the EZ layer on the en face image in the right eye. We diagnosed the patient with acute zonal occult outer retinopathy. Visual field tests and B-scan images improved to almost normal at 6 months, but hyporeflective areas remained on the en face images. Thus, en face images may be more sensitive at detecting abnormalities in the outer retina than other modalities.


Asunto(s)
Síndromes de Puntos Blancos , Humanos , Femenino , Adulto Joven , Adulto , Estudios de Seguimiento , Síndromes de Puntos Blancos/diagnóstico , Tomografía de Coherencia Óptica , Retina/diagnóstico por imagen
9.
Retina ; 42(4): 704-711, 2022 04 01.
Artículo en Inglés | MEDLINE | ID: mdl-34983900

RESUMEN

PURPOSE: To establish an objective and quantitative biomarker of metamorphopsia in epiretinal membranes (ERMs) and determine the optimal timing for ERM surgery. METHODS: Retrospectively, 172 eyes with ERM were reviewed. Retinal folds because of tangential traction by ERM were visualized by en-face optical coherence tomography. The maximum depth of retinal folds (MDRF) within the parafovea was quantified. Metamorphopsia was quantified by M-CHARTS. The change in the distance between the retinal vessels after ERM surgery and the preoperative total depth of retinal folds between the vessels were quantified using en-face optical coherence tomography and optical coherence tomography angiography. RESULTS: Significant correlations were observed between preoperative MDRF and M-CHARTS scores before and at 6 months after surgery (r = 0.617 and 0.460, respectively; P < 0.001) and change in the distance between the retinal vessels after ERM surgery and preoperative total depth of retinal folds between the vessels (r = 0.471; P = 0.013). The preoperative MDRF values at which M-CHARTS scores were 0.5 before and 6 months after the surgery were 69 µm and 118 µm, respectively. CONCLUSION: The MDRF is an objective and quantitative biomarker of metamorphopsia in ERM. To maintain patients' quality of vision, ERM surgery may be performed when the preoperative MDRF ranges between 69 µm and 118 µm.


Asunto(s)
Membrana Epirretinal , Biomarcadores , Membrana Epirretinal/complicaciones , Membrana Epirretinal/cirugía , Humanos , Retina , Estudios Retrospectivos , Tomografía de Coherencia Óptica/métodos , Trastornos de la Visión/etiología , Agudeza Visual , Vitrectomía/métodos
10.
Acta Med Okayama ; 76(3): 349-354, 2022 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-35790368

RESUMEN

An 18-year-old male high school student presented to our clinic with bilateral blunt ocular trauma caused by an exercise resistance band (ERB) during a muscle-building exercise. Best-corrected decimal visual acuities (BCVAs) for right and left eyes were light perception and 0.15, respectively. The right eye was operated 10 days after injury for persistent vitreous hemorrhage, and the left eye 5 months later because of macular hole onset. After 36 months, the right eye showed extensive retinal degeneration (BCVA 0.04), and the left eye macular hole closure (BCVA 1.2). ERBs should be used cautiously as they can cause serious ocular trauma.


Asunto(s)
Lesiones Oculares , Perforaciones de la Retina , Heridas no Penetrantes , Adolescente , Lesiones Oculares/etiología , Humanos , Masculino , Músculos , Natación , Agudeza Visual , Heridas no Penetrantes/complicaciones
11.
Graefes Arch Clin Exp Ophthalmol ; 259(9): 2503-2512, 2021 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-33710473

RESUMEN

PURPOSE: To investigate epiretinal membrane (ERM) formation using en face optical coherence tomography (OCT) after vitrectomy for rhegmatogenous retinal detachment (RRD). METHODS: We retrospectively reviewed the medical records of 64 consecutive eyes (64 patients) with RRD treated by vitrectomy without ERM and internal limiting membrane peeling. ERMs and retinal folds were detected by B-scan and en face imaging. The maximum depth of retinal folds (MDRF) was quantified using en face imaging. ERM severity was staged using B-scan imaging. Main outcome measures were ERM detection rate with B-scan and en face imaging, MDRF, ERM staging, postoperative best-corrected visual acuity (BCVA; logarithm of the minimum angle of resolution), and risk factors for ERM formation. RESULTS: The detection rate for ERM formation was significantly higher with en face imaging (70.3%) than with B-scan imaging (46.9%; P = 0.007). There was no significant difference in postoperative BCVA between eyes with ERM formation (0.06 ± 0.26) and those without ERM formation (0.01 ± 0.14; P = 0.298). Forty of 45 (88.9%) eyes with ERM formation were classified as stage 1. Twenty-seven of 45 (60.0%) eyes with ERM formation developed parafoveal retinal folds. The mean MDRF was 27.4 ± 32.2 µm. Multiple retinal breaks and a maximum retinal break size of ≥ 2 disc diameters were significantly associated with ERM formation (P = 0.033 and P = 0.031, respectively). CONCLUSION: Although ERM formation was observed in 70.3% patients after RRD repair, the formed ERM was not severe and had minimal impact on the postoperative visual acuity.


Asunto(s)
Membrana Epirretinal , Desprendimiento de Retina , Membrana Epirretinal/diagnóstico , Membrana Epirretinal/cirugía , Humanos , Desprendimiento de Retina/diagnóstico , Desprendimiento de Retina/cirugía , Estudios Retrospectivos , Tomografía de Coherencia Óptica , Vitrectomía
12.
Acta Med Okayama ; 75(3): 391-395, 2021 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-34176945

RESUMEN

A 58-year-old Japanese man underwent vitrectomy for rhegmatogenous retinal detachment (RRD) in 2002. Twelve years later, optical coherence tomography revealed the development of a lamellar macular hole; the visual acuity was 20/200. Two years later, because metamorphopsia and the foveal retina thinning were aggravated, epiretinal proliferation embedding was performed to restore the foveal structure by transplanting glial cells to the foveal cavity. The patient was followed-up for 4 years, and his macular morphology and visual acuity (20/66) improved. No complications occurred. This appears to be the first report of epiretinal proliferation embedding for a lamellar macular hole post-RRD repair.


Asunto(s)
Proliferación Celular , Membrana Epirretinal/cirugía , Perforaciones de la Retina/cirugía , Humanos , Masculino , Persona de Mediana Edad , Recurrencia , Desprendimiento de Retina/cirugía , Perforaciones de la Retina/diagnóstico por imagen , Perforaciones de la Retina/patología , Tomografía de Coherencia Óptica , Vitrectomía/efectos adversos
13.
Acta Med Okayama ; 75(2): 249-253, 2021 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-33953434

RESUMEN

A 79-year-old woman presented to our hospital with a 10-day history of gradually worsening binocular vision and severe backache. Further investigations revealed poor bilateral best-corrected visual acuities (BCVA), bilateral vitreous opacities, gray-white lesions scattered throughout the retina, and a left iliopsoas abscess on CT that later grew out methicillin-sensitive S. aureus. The abscess was drained and intravenous antibiotics were initiated, but the left eye additionally required intravitreal vancomycin. BCVA for both eyes normalized within 1 year. Intramuscular abscess should be considered as a possible primary lesion in cases of endogenous bacterial endophthalmitis.


Asunto(s)
Endoftalmitis/diagnóstico , Infecciones Bacterianas del Ojo/diagnóstico , Absceso del Psoas/diagnóstico , Anciano , Femenino , Humanos , Staphylococcus aureus Resistente a Meticilina/aislamiento & purificación , Infecciones Estafilocócicas/diagnóstico
14.
Acta Med Okayama ; 75(6): 741-744, 2021 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-34955543

RESUMEN

A 65-year-old man presented with a 1-week history of left eye distortion. An elevated choroidal lesion covering 6 disc diameters was found in the posterior retina of the left eye. Systemic examination revealed sublingual gland carcinoma and multiple lung metastases, and the diagnosis was choroidal metastasis from sublingual gland carcinoma. Following chemotherapy and radiation therapy, the choroidal lesion shrunk and the patient's visual acuity improved. The patient died 23 months after his first visit. To the best of our knowledge, this is the first reported case of choroidal metastasis from sublingual gland carcinoma.


Asunto(s)
Carcinoma/patología , Neoplasias de la Coroides/secundario , Neoplasias de la Glándula Sublingual/patología , Anciano , Carcinoma/diagnóstico , Resultado Fatal , Humanos , Neoplasias Pulmonares/secundario , Masculino , Glándula Sublingual/patología , Neoplasias de la Glándula Sublingual/diagnóstico
15.
Mol Cell ; 45(4): 494-504, 2012 Feb 24.
Artículo en Inglés | MEDLINE | ID: mdl-22365829

RESUMEN

Cell cycle-dependent expression of canonical histone proteins enables newly synthesized DNA to be integrated into chromatin in replicating cells. However, the molecular basis of cell cycle-dependency in the switching of histone gene regulation remains to be uncovered. Here, we report the identification and biochemical characterization of a molecular switcher, HERS (histone gene-specific epigenetic repressor in late S phase), for nucleosomal core histone gene inactivation in Drosophila. HERS protein is phosphorylated by a cyclin-dependent kinase (Cdk) at the end of S-phase. Phosphorylated HERS binds to histone gene regulatory regions and anchors HP1 and Su(var)3-9 to induce chromatin inactivation through histone H3 lysine 9 methylation. These findings illustrate a salient molecular switch linking epigenetic gene silencing to cell cycle-dependent histone production.


Asunto(s)
Proteínas de Drosophila/fisiología , Drosophila/genética , Epigénesis Genética , Regulación de la Expresión Génica , Silenciador del Gen , Histonas/genética , Proteínas Represoras/fisiología , Animales , Ciclo Celular , Proteínas de Drosophila/genética , Proteínas de Drosophila/metabolismo , Fosforilación , Regiones Promotoras Genéticas , Proteínas Represoras/genética , Proteínas Represoras/metabolismo , Fase S
16.
Retina ; 40(5): 989-997, 2020 May.
Artículo en Inglés | MEDLINE | ID: mdl-30649079

RESUMEN

PURPOSE: To investigate the clinical course of submacular hemorrhage associated with ruptured retinal arterial macroaneurysm using swept-source optical coherence tomography. METHODS: This study included 23 eyes of 23 consecutive patients diagnosed with submacular hemorrhage associated with ruptured retinal arterial macroaneurysm. Cases underwent displacement of submacular hemorrhage (vitrectomy + subretinal injection of tissue plasminogen activator + air tamponade) and were followed up for 6 months after surgery. Localization of the preoperative hemorrhage and its effect on preoperative and postoperative best-corrected visual acuity, central retinal thickness, and continuity of the ellipsoid zone were measured. RESULTS: Macular intraretinal hemorrhage (IRH) was observed in 17 eyes (73.9%, IRH [+] group) and was not observed in 6 eyes (26.1%, IRH [-] group). The IRH (+) group showed worse postoperative best-corrected visual acuity values compared with the IRH (-) group (0.89 ± 0.47 in logarithm of the minimal angle of resolution units, Snellen equivalent 20/155 and 0.16 ± 0.23, 20/29, respectively; P < 0.01), smaller central retinal thickness values (97.7 ± 53.5 µm, 173.0 ± 32.3 µm, respectively; P < 0.01), and a higher rate of ellipsoid zone disruption (100%, 33.3%, respectively; P < 0.01). CONCLUSION: Patients with preoperative macular IRH showed lower postoperative visual acuity and worse macular contour after submacular hemorrhage displacement compared with patients without macular IRH.


Asunto(s)
Aneurisma Roto/complicaciones , Angiografía con Fluoresceína/métodos , Retina/patología , Macroaneurisma Arterial de Retina/complicaciones , Hemorragia Retiniana/diagnóstico , Tomografía de Coherencia Óptica/métodos , Agudeza Visual , Anciano de 80 o más Años , Aneurisma Roto/diagnóstico , Femenino , Estudios de Seguimiento , Fondo de Ojo , Humanos , Masculino , Pronóstico , Macroaneurisma Arterial de Retina/diagnóstico , Hemorragia Retiniana/etiología , Estudios Retrospectivos
17.
Retina ; 40(7): 1262-1271, 2020 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-31136461

RESUMEN

PURPOSE: To evaluate the relationship between retinal traction caused by epiretinal membrane and visual functions. METHODS: In this institutional study, en face swept-source optical coherence tomography images of 141 eyes of 130 patients with epiretinal membrane were analyzed to investigate maximum depth of retinal folds, which represents retinal traction strength and the distribution pattern of retinal folds. We investigated the relationships between the maximum depth and distribution pattern of retinal folds and visual functions as well as the effects of membrane peeling. RESULTS: Maximum retinal fold depth was significantly correlated with the metamorphopsia score (P < 0.001). Fifteen eyes showed retinal folds radially extending from the macular epiretinal membrane (radiating folds group), whereas 126 eyes showed a multidirectional pattern of retinal folds (multidirectional folds group). The radiating folds group showed a significantly lower metamorphopsia score (P = 0.014). Multiple regression analysis revealed that the metamorphopsia score was significantly related to maximum retinal fold depth (P = 0.003), distribution pattern (P = 0.015), and central retinal thickness (P < 0.001). One month after membrane peeling, parafoveal retinal folds resolved completely in all cases, and both visual acuity (P < 0.001) and average metamorphopsia score (P = 0.036) were significantly improved. CONCLUSION: Both the strength and the distribution pattern of retinal traction are significantly related to metamorphopsia in epiretinal membrane patients.


Asunto(s)
Membrana Epirretinal/diagnóstico , Mácula Lútea/patología , Tomografía de Coherencia Óptica/métodos , Trastornos de la Visión/diagnóstico , Agudeza Visual/fisiología , Anciano , Membrana Epirretinal/complicaciones , Membrana Epirretinal/fisiopatología , Femenino , Estudios de Seguimiento , Humanos , Masculino , Pronóstico , Estudios Retrospectivos , Trastornos de la Visión/etiología , Trastornos de la Visión/fisiopatología
18.
Retina ; 40(10): 1873-1880, 2020 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-31764614

RESUMEN

PURPOSE: To visualize foveal microstructures in macula-off rhegmatogenous retinal detachment using swept source optical coherence tomography preoperatively and postoperatively and to investigate the relationship between foveal microstructures and postoperative visual acuity. METHODS: We retrospectively analyzed 42 eyes of 42 consecutive patients diagnosed with macula-off rhegmatogenous retinal detachment who underwent anatomically successful repair surgery and were followed up for 6 months. We used swept source optical coherence tomography to investigate the relationship between preoperative and postoperative continuity of both the external limiting membrane (ELM) and ellipsoid zone (Ez) and preoperative and postoperative best-corrected visual acuity. RESULTS: Both preoperative ELM and Ez were continuous in 9 eyes (21%; ELM+/Ez+ eyes), only the ELM was continuous in 25 eyes (60%; ELM+/Ez- eyes), and neither was continuous in 8 eyes (19%; ELM-/Ez- eyes). Postoperative best-corrected visual acuity in ELM+/Ez+ eyes (-0.05 ± 0.04 logarithm of the minimum angle of resolution units, Snellen equivalent 20/18) was significantly better than that in both ELM+/Ez- (0.16 ± 0.16, 20/29; P = 0.03) and ELM-/Ez- (0.86 ± 0.37, 20/145; P < 0.001) eyes. Postoperative best-corrected visual acuity was significantly better in ELM+/Ez- than in ELM-/Ez- eyes (P < 0.001). CONCLUSION: In macula-off rhegmatogenous retinal detachment, preoperative continuity of the ELM and Ez may be a predictor of postoperative best-corrected visual acuity.


Asunto(s)
Fóvea Central/diagnóstico por imagen , Desprendimiento de Retina/diagnóstico por imagen , Agudeza Visual/fisiología , Adulto , Anciano , Membrana Basal/diagnóstico por imagen , Endotaponamiento , Femenino , Fóvea Central/fisiopatología , Humanos , Masculino , Persona de Mediana Edad , Periodo Preoperatorio , Desprendimiento de Retina/fisiopatología , Desprendimiento de Retina/cirugía , Estudios Retrospectivos , Curvatura de la Esclerótica , Tomografía de Coherencia Óptica , Vitrectomía
19.
BMC Ophthalmol ; 20(1): 427, 2020 Oct 23.
Artículo en Inglés | MEDLINE | ID: mdl-33097007

RESUMEN

BACKGROUND: Perfluorocarbon liquid (PFCL) is an effective surgical adjuvant in performing vitrectomy for severe vitreoretinal pathologies such as proliferative vitreoretinopathy and giant retinal tears. However, subretinal retention of PFCL can occur postoperatively and retained PFCL causes severe visual disorders, particularly when PFCL was retained under the fovea. Although several procedures have been proposed for subfoveal PFCL removal, such as direct aspiration or submacular injection of balanced salt solution (BSS) to dislodge the subfoveal PFCL, the retinal damage associated with these procedures has been a major problem. Here, we report a case of subfoveal retention of PFCL for which we performed a novel surgical technique that attempts to minimize retinal damage. CASE PRESENTATION: A 69-year-old man presented with subfoveal retained PFCL after surgery for retinal detachment. To remove the retained PFCL, the internal limiting membrane overlying the subretinal injection site is first peeled to allow low-pressure (8 psi) transretinal BSS infusion, using a 41-gauge cannula, to slowly detach the macula. A small drainage retinotomy is created with the diathermy tip at the inferior position of the macular bleb, sized to be slightly wider than that of the PFCL droplet. The head of the bed is then raised, and the surgeon gently vibrates the patient's head to release the PFCL droplet to allow it to migrate inferiorly towards the drainage retinotomy. The bed is returned to the horizontal position, and the PFCL, now on the retinal surface, can be aspirated. The subfoveal PFCL is removed while minimizing iatrogenic foveal and macular damage. One month after PFCL removal, the foveal structure showed partial recovery on optical coherence tomography, and BCVA improved to 20/40. CONCLUSION: Creating a macular bleb with low infusion pressure and using vibrational forces and gravity to migrate the PFCL towards a retinotomy can be considered as a relatively atraumatic technique to remove subfoveal retained PFCL.


Asunto(s)
Fluorocarburos , Desprendimiento de Retina , Perforaciones de la Retina , Anciano , Humanos , Masculino , Desprendimiento de Retina/cirugía , Perforaciones de la Retina/cirugía , Vibración , Vitrectomía
20.
J Biol Chem ; 293(6): 2206-2218, 2018 02 09.
Artículo en Inglés | MEDLINE | ID: mdl-29233889

RESUMEN

Feedback control is a key mechanism in signal transduction, intimately involved in regulating the outcome of the cellular response. Here, we report a novel mechanism by which PHLDA1, Pleckstrin homology-like domain, family A, member 1, negatively regulates ErbB receptor signaling by inhibition of receptor oligomerization. We have found that the ErbB3 ligand, heregulin, induces PHILDA1 expression in MCF-7 cells. Transcriptionally-induced PHLDA1 protein directly binds to ErbB3, whereas knockdown of PHLDA1 increases complex formation between ErbB3 and ErbB2. To provide insight into the mechanism for our time-course and single-cell experimental observations, we performed a systematic computational search of network topologies of the mathematical models based on receptor dimer-tetramer formation in the ErbB activation processes. Our results indicate that only a model in which PHLDA1 inhibits formation of both dimers and tetramer can explain the experimental data. Predictions made from this model were further validated by single-molecule imaging experiments. Our studies suggest a unique regulatory feature of PHLDA1 to inhibit the ErbB receptor oligomerization process and thereby control the activity of receptor signaling network.


Asunto(s)
Receptor ErbB-3/metabolismo , Factores de Transcripción/metabolismo , Humanos , Células MCF-7 , Modelos Químicos , Neurregulina-1/metabolismo , Multimerización de Proteína , Transducción de Señal , Imagen Individual de Molécula , Análisis de la Célula Individual , Factores de Transcripción/fisiología , Transcripción Genética
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