Clinical value of the expression levels of tumor protein D52 and miR-133a on prognosis assessment of pancreatic cancer surgery.

Objective: To investigate the clinical value of the expression levels of tumor protein D52 (TPD52) and miR-133a on the prognosis assessment of pancreatic cancer surgery. Methods: This was a retrospective study. Ninety-seven patients who underwent radical surgery for pancreatic cancer in Cangzhou Central Hospital from January 2018 to January 2022 were selected and divided into four groups: TPD52 high expression group, TPD52 low expression group, miR-133a high expression group and miR-133a low expression group. The relationship between the expression levels of TPD52 and miR-133a and the clinicopathological features of patients with pancreatic cancer was analyzed. The COX regression model was used to analyze the risk factors affecting the prognosis of patients with pancreatic cancer. Results: The high expression rate of TPD52 and the low expression rate of miR-133a in pancreatic cancer tissues were higher than those in normal paracancerous tissues(P<0.05). Based on the comparison of prognosis and survival, the median survival time of patients with high expression of TPD52 and low expression of miR-133a was lower than that of patients with low expression of TPD52 and high expression of miR-133a, with a statistically significant difference(P<0.05). Moreover, multivariate Cox regression analysis showed that low differentiation of pancreatic cancer, III-IV stage of TNM, high expression of TPD52, as well as low expression of miR-133a were independent risk factors for postoperative survival of patients with pancreatic cancer(P<0.05). Conclusion: TPD52 is expressed at a high level whereas miR-133a at a low level in pancreatic cancer tissues, both of which together with low differentiation of pancreatic cancer and III-IV stage of TNM constitute independent risk factors affecting the surgical prognosis of patients with pancreatic cancer.

[Spatiotemporal Response of Ecosystem Service Value to Land Use Change in the Lanzhou-Xining Urban Agglomeration over the Past 20 Years].

With rapid urbanization and human activities exacerbating threats to the degradation of various ecosystem services in modern urban agglomerations, the exploration of the state of ecological security at the scale of urban agglomerations is of great significance. This study considered the Lanzhou-Xining Urban Agglomeration as the research area, based on the land use data in 2000, 2005, 2010, 2015, and 2020. At the same time, the landscape ecological risk index was introduced. The land use change characteristics of the Lanzhou-Xining Urban Agglomeration were analyzed by using the land use transfer matrix, the value per unit area equivalent factor method, and the bivariate spatial autocorrelation analysis method to elucidate the impacts of the changes in the ecological risk index induced by the land use transition on the value of ecosystem services. This study analyzed the land use change characteristics of the Lanzhou-Xining Urban Agglomeration and elucidated the impacts of changes in the ecological risk index on the value of ecosystem services caused by land use transformation. The results showed that:① During the period from 2000 to 2020, the land use types of the Lanzhou-Xining Urban Agglomeration were mainly dominated by grassland, cropland, and forest land. The construction land area had expanded significantly mainly from cropland and grassland, and the six land use types had strong cross-transformation. The total area of land use change was 6 646.05 km2. ② In terms of spatial changes, the spatial pattern of ecosystem service value in the Lanzhou-Xining Urban Agglomeration had not undergone obvious transformation. However, the regional variability was significant, generally showing the distribution characteristics of high in the northwest and low in the southeast. ③From the perspective of temporal change, the value of ecosystem services in the Lanzhou-Xining Urban Agglomeration showed an upward trend, with the total flow of value increasing from 186.459 billion yuan to 192.156 billion yuan, with a total value-added of 5.697 billion yuan. ④ There was a rising trend in the overall ecological risk index of the Lanzhou-Xining Urban Agglomeration over the past 20 years. Low ecological risk areas and lower ecological risk areas dominated the ecological risk areas. There was a significant positive correlation between the value of ecosystem services and the ecological risk index. This study aimed to reveal the understanding of the impacts of land-use practices on ecosystem service values and ecological risks, to provide important references for regional ecological risk management and land-use policy formulation, and thus to promote the high-quality development of the ecological environment in the Yellow River Basin.

[Temporal and Spatial Distributions of O3 Concentration and Potential Source Area Analysis of Hexi Corridor Based on Satellite and Ground Monitoring].

Based on 2005-2020 O3 column concentration data of OMI remote sensing satellite, combined with air pollutant data from 10 nationally controlled environmental automatic monitoring stations in the Hexi Corridor and global data assimilation system meteorological data, we used Kriging interpolation, correlation analysis, and backward trajectory (HYSPLIT) models to explore the temporal and spatial distribution characteristics, meteorological factors, transmission paths, and potential sources of O3 in the Hexi Corridor. The results showed the following:① in terms of temporal distribution, O3 column concentration showed an upward trend in 2005-2010 and 2014-2020 and downward trend in 2010-2014; the maximum and minimum values were reached in 2010 and 2014 (332.31 DU and 301.00 DU), respectively, and seasonal changes showed that those in spring and winter were significantly higher than those in summer and autumn. ② In terms of spatial distribution, O3 column concentration showed a latitudinal band distribution characteristic of increasing from southwest to northeast; the high-value areas were primarily distributed in urban areas with low terrain, and the median zone was latitudinally striped with the basic alignment of the Qilian foothills. ③ The analysis of meteorological conditions revealed that temperature, wind speed, and sunshine hours were positively correlated with O3, and relative humidity was negatively correlated with O3. ④ By simulating the airflow transportation trajectory of the receiving point in Wuwei City, it was found that the direction of the O3 conveying path was relatively singular; the dominant airflow in each season was primarily in the west and northwest; and the proportions were 71.62%, 66.85%, 61.22%, and 77.78%, respectively. There were certain seasonal differences in the source areas of O3 potential contribution:the high-value areas of O3 potential sources in spring, summer, and autumn were distributed in Baiyin City and Lanzhou City, which were southeast wind sources, and the high-value areas in winter were distributed between the Badain Jaran Desert and the Tengger Desert, which was the north wind source.

[Effects of Greenhouse and Open-field Cultivation on Heavy Metal Uptake During Carrot Growth].

Clarifying the absorption dynamics of heavy metal(loid)s by crops under different cultivation methods is critical for risk management and control for heavy metal pollution. Here, taking carrots as an example, the pH, cation exchange capacity (CEC), and contents of heavy metals in soil and carrots were analyzed. We compared the absorption and transport characteristics of six metal(loid)s (As, Cd, Cr, Cu, Pb, and Zn) during the three key growth periods under greenhouse and open-field cultivation methods. In addition, the effects of planting methods on carrot biomass and heavy metal content over time were studied, and a health risk assessment was conducted. The results showed that the greenhouse and open-field cultivation methods had the following in common:① As the carrots continuously grew, the metal uptake and biomass in the belowground part (edible part) and the metal(loid) concentrations in the aboveground and belowground parts both showed trends of increasing first and then decreasing or stabilizing. ② The absorption of As, Cd, Cr, and Pb in carrots was mostly accumulated in the aboveground part. ③ The content of Cd in the edible part exceeded the standard, and the total target hazard quotient was>1, indicating potential adverse health risks, most of which were contributed by As and Cd. Compared to that under open-field, the short-term greenhouse cultivation had a harvest time approximately 15 d earlier. The As, Cd, and Pb concentrations in the aboveground part during the seedling and fast-growing periods were significantly lower in the greenhouse than that in the open-field (P<0.05) but did not differ between greenhouse and open-field (except As) at the mature stage. The concentrations of As and Cd in the belowground part of greenhouse carrots were lower in the greenhouse than those of carrots grown in the open-field at the seedling stage (P<0.05). The absorption of As and Cd was decreased significantly from 0 to 95 d (P<0.05), and there was no significant difference in the metal concentration and absorption in the rest of the growth stages. The bioconcentration factors (BCF) of As, Cd, and Pb in the aboveground part in all stages and those of As and Cd in the belowground part in the seedling stage were lower in the greenhouse than that in the open-field. This study emphasizes the possibility of short-term changes in cultivation mode to reduce the risks of metal(loid)s in contaminated farmland vegetables.

LncRNA DANCR regulates the growth and metastasis of oral squamous cell carcinoma cells via altering miR-216a-5p expression.

The study aims to investigate how DANCR can alter the growth and metastasis of oral squamous cell carcinoma (OSCC) cells by regulating miR-216a-5p. The expression of DANCR and miR-216a-5p in OSCC patients and cells were measured. SCC15 and CAL-27 cells were selected to divide into Control, sh-NC, DANCR shRNA, DANCR, miR-216a-5p mimic, and DANCR + miR-216a-5p mimic groups. Dual-luciferase reporter gene assay was performed for the verification of the targeting relationship between miR-216a-5p and DANCR/Bcl-2/KLF12. We also quantified the abilities of OSCC cells regarding proliferation, invasion, migration and apoptosis, and the expression levels of apoptosis-related proteins were measured. Finally, the tumor-bearing nude mice were established to verify the effect of DANCR in vivo. Up-regulated DANCR expression and down-regulated miR-216a-5p expression were observed in both OSCC tissues and cells, and they were proven strongly correlated to the histological grade, clinical staging and lymph node metastasis of OSCC patients. Dual-luciferase reporter gene assay showed a target relationship between DANCR and miR-216a-5p, as well as between miR-216a-5p and Bcl-2/KLF12. Both DANCR shRNA and miR-216a-5p mimic decreased proliferative, migration and invasive abilities of OSCC cells with increased cell apoptosis. However, DANCR group showed completely opposite trends. Moreover, miR-216a-5p mimic could reverse the role of DANCR in promoting tumor growth. In-vivo experiment confirmed the inhibitory role of DANCR shRNA in tumor growth and metastasis. We concluded that DANCR may promote the growth and metastasis of OSCC cells and suppress OSCC cell apoptosis by sponging miR-216a-5p.

[Lymph nodes amyloidosis of cervical: a case report].

There were many masses on a patient's cervical region which caused significant respiratory compression. After aspiration and incisional biopsy, the results confirmed the diagnosis of local lymph nodes amyloidosis.