RESUMEN
Some recent studies have reviewed the occurrence and phytotoxicity of micro/nanoplastics, but their distribution in the soil environment, mechanisms of uptake by roots and the mode of action are unclear. Thus, this review comprehensively represents the relative abundance of micro/nanoplastics in different soil types and their toxicities in plants with insights into their partitioning to different soil matrices, uptake mechanisms, and the mode of action. Partitioning of micro/nanoplastics to different soil matrices (like-soil particles, naturally occurring soil organic matters, pore waters and soil fauna) could modify their bioavailability to plants. The small micro/nanoplastic particles can be taken up by roots through the apoplastic and symplastic pathways. In this regard, cellular endocytosis and aquaporin might play a significant role. The shape of the polymers can also regulate their uptake, and the polymers with spherical shapes are more easily absorbed by roots than the polymers with other shapes. Bioaccumulation of micro/nanoplastic induces oxidative stress, which, in turn, causes alterations of gene expressions and different metabolic pathways responsible for plant growth, biomass production and synthesis of secondary metabolites.
Asunto(s)
Contaminantes del Suelo , Suelo , Microplásticos , Raíces de Plantas/metabolismo , Plantas/metabolismo , Contaminantes del Suelo/metabolismoRESUMEN
Escalated production of plastic, their worldwide distribution and persistent nature finally results into their environmental accumulation causing severe threats to the ecological environment and biotic health. Thus, development of suitable measurements for environmental remediation of plastic may be an urgent issue in this plastic age. Some recent reviews have categorized the microbial species able to degrade different plastic polymers and the different factors effecting bio-degradation of plastic are poorly understood. This review comprehensively discusses bio-degradation of traditional and biodegradable plastic polymers both in natural and biological environment (gut microbes and fungi) to understand different factors regulating their degradation, and also shows how degradation of plastic polymers under abiotic factors influence subsequent biological degradation. Different physicochemical modifications like - breaking large polymers into small fragments by pre-treatment, functional groups enrichment, identifying potent microbial species (consortia) and engineering microbial enzymes might be crucial for bio-degradations of plastic. Effects of micro/nanoplastic and other chemical intermediates, formed during the bio-degradation of plastic, on species composition, abundance, growth, metabolism and enzymatic systems of microbes involved in the bio-degradation of plastic should be determined in future research.
Asunto(s)
Plásticos , Polímeros , Biodegradación Ambiental , HongosRESUMEN
Plastic pollution has become a global concern for ecosystem health and biodiversity conservation. Concentrations of plastics are manifold higher in the terrestrial system than the aquatic one. Micro/nanoplastics (M/NP) have the ability to alter soil enzymatic system, soil properties and also affect soil borne microorganisms and earthworms. Despite, the knowhow regarding modulatory effects of plastics are acquired from the study on aquatic system and reports on their phytotoxic potentials are limited. The presence of cell wall that could restrict M/NP invasion into plant roots might be the putative cause of this limitation. M/NP inhibit plant growth, seed germination and gene expression; and they also induce cytogenotoxicity by aggravating reactive oxygen species generation. Dynamic behavior of cell wall; the pores formed either by cell wall degrading enzymes or by plant-pathogen interactions or by mechanical injury might facilitate the entry of into roots M/NP. This review also provides a possible mechanism of large sized microplastics-induced phytotoxicity especially for those that cannot pass through cell wall pores. As M/NP affect soil microbial community and soil parameters, it is hypothesized that they could have the potential to affect N2 fixation and research should be conducted in this direction. Reports on M/NP-induced toxicity mainly focused only on one polymer type (polystyrene) in spite of the toxicological relevancies of other polymer types like polyethylene, polypropylene etc. So, the assessment of phytotoxic potential of M/NP should be done using other plastic polymers in real environment as they are known to intract with other environmental stressors as well as can alter the the soil-microbe-plant interaction.
Asunto(s)
Ecosistema , Plásticos , Contaminación Ambiental , Microplásticos , SueloRESUMEN
Tin-based TMDCs are gaining traction in optoelectronics due to their eco-friendliness and easy synthesis, contrasting Mo/W-based counterparts. This study pioneers the solvothermal synthesis of highly crystalline SnSSe alloy, akin to Janus structures, bridging a notable research gap. By integrating SnS2/SnSSe materials onto a GaN platform, a synergistic heterojunction is created, enhancing light absorption and the electron-hole pair separation efficiency, demonstrating a self-powered photodetection. The GaN/SnS2/SnSSe heterojunction showcases a staircase-like (type-II) band alignment and exceptional performance metrics: high photoresponsivity of 314.96 A W-1, specific detectivity of 2.0 × 1014 jones, and external quantum efficiency of 10.7 × 104% under 365 nm illumination at 150 nW cm-2 intensity and 3 V bias. Notably, the device displays intensity-dependent photocurrent and photoswitching behaviors without external bias, highlighting its unique self-powered attributes. This study underscores SnS2's significance in optoelectronics and explores SnSSe integration into van der Waals heterostructures, promising advanced photodetection devices and bias-free optoelectronics.
RESUMEN
Broadband photodetection including deep UV using Si is technically challenging due to its negligible optical absorption at 254 nm and the requirement of heterogeneous integration with very high bandgap photoactive materials. However, monolithic integration of high-bandgap semiconductors on Si is not possible due to CMOS fabrication incompatibility. Comprehensive experimental studies to achieve broadband photodetection including deep UV on Si are lacking in the literature. Here for the first time we have investigated 2D/0D heterojunctions of WS2/WO3 on a Si platform both experimentally and theoretically and established the charge transfer mechanism between them. Transient photocarrier decay experiments demonstrate effective quenching of excited photocarriers generated in WO3/WS2, signifying its utility in facilitating carrier transport, which is further evidenced by charge density calculation from DFT simulation. Our designed vertically aligned p-Si/WS2/WO3 heterojunction-based photodetector exhibits an excellent photosensitivity performance with a broad spectral response ranging from deep ultraviolet (254 nm) to near infrared (940 nm) wavelengths, and it not only provides a peak responsivity of 251 A W-1 and a specific detectivity of 1.89 × 014 Jones, but also possesses a rapid response speed with a rise/fall time of 0.64/0.48 s at 365 nm with a bias of 2 volt.
RESUMEN
Plastic pollution has been reported to negatively impact global biodiversity and ecosystem health. However, the molecular mechanisms of nano-plastics in plants are unidentified, especially their negative impacts on genomic stability. This study for the first time showed that nano-polystyrene leads to cell death in plants by subjugating the cellular antioxidant defence mechanisms through the aggravated production of ROS, which in turn could induce the DNA damage impairing the genetic regulation of the corresponding DNA repair pathway. To validate the proposed hypothesis, the DNA damage potential of nano-polystyrene and the expression levels of key genetic regulators of the DNA damage repair pathway (such as - CYCA/B, CDKA, SOG1, MYB transcription factors, and RAD51) have been assessed in onion roots after 72 h exposure with three ecologically relevant concentrations (25, 50, and 100 µg ml-1) of 100 nm nano-polystyrene. In addition, imbalance in redox homeostasis (oxidative stress), cell viability, and nuclear aberrations such as - the frequency of micronucleus and bi-nucleate cells that are directly linked to the DNA damages have been checked to point out the cause and effect of nano-polystyrene-induced DNA damage. Results showed a significant increase in oxidative stress in each treatment concentrations of nano-polystyrene. However, ROS generated at 100 µg ml-1 nano-polystyrene dose subdues the antioxidant defence system and induces cell death. These observations may be ascribed to the accumulation damaged DNA and the down-regulation of repair pathway-associated genes, as observed in this treatment group. Conversely, the observed DNA damage and the reduced expressions of genes would be a mere consequence of reduced cellular viability.
Asunto(s)
Cebollas , Poliestirenos , Poliestirenos/metabolismo , Especies Reactivas de Oxígeno/metabolismo , Antioxidantes/metabolismo , Ecosistema , Daño del ADN , Estrés Oxidativo , Muerte CelularRESUMEN
Melatonin is an important biomolecule found in diverse groups of organisms. Under different abiotic stresses, the synthesis of melatonin is markedly increased suggesting pivotal roles of melatonin in plants enduring stresses. Being an endogenous signaling molecule with antioxidant activity, melatonin alters many physiological responses and is found to be involved in regulating DNA damage responses. However, the molecular mechanisms of melatonin in response to DNA damage have not yet been studied. The present review aims to provide insights into the molecular mechanisms of melatonin in response to DNA damage in plants. We propose that the MAP kinase pathway is involved in regulating melatonin dependent response of plants under DNA damage stress. Where melatonin might activate MAPK via H2 O2 or Ca2+ dependent pathways. The activated MAPK in turn might phosphorylate and activate SOG1 and repressor type MYBs to mitigate DNA damage under abiotic stress.
Asunto(s)
Melatonina , Antioxidantes/farmacología , Daño del ADN , Regulación de la Expresión Génica de las Plantas , Melatonina/metabolismo , Melatonina/farmacología , Proteínas Quinasas Activadas por Mitógenos/genética , Proteínas Quinasas Activadas por Mitógenos/metabolismo , Estrés Fisiológico/genéticaRESUMEN
Many studies have investigated the negative impacts of microplastics on teleost fishes with very little or no evidence of their mechanism of action. This scenario entreats us to investigate the toxicities of nanopolystyrene in zebrafish oocyte with emphasis on the mechanism of action. In the present study, the cellular levels of mRNA transcripts of different genetic markers (such as: sod, gpx, nrf2, inos, ucp2, and atp6 (redox-sensitive markers); nfkß, tnfα, il-10, ikß, gdf9, and bmp15 (immune markers); gadd45, rad51, p53 and bcl2 (DNA damage and apoptotic)) have been quantified by real-time PCR after 6 h of incubation of isolated oocyte with different doses of nanopolystyrene viz. P0 (control i.e. no polystyrene in culture medium), P1 (100 ng/ml), and P2 (400 ng/ml). Results showed that both the treatment concentrations of nanopolystyrene induce oxidative stress with % DPPH = 30.75, 31.61, and 32.43% for P0, P1, and P2, respectively. Increase in oxidative stress in oocytes with increasing doses of nanopolystyrene was also observed in TBARS assay with MDA content 0.12 and 0.21 µM for P1 and P2, respectively as compaired to the control 0.08 µM. This increased oxidative stress can regulate the expression pattern (upregulation/downregulation) of selected genes leading to different toxic effects like - oxidative stress, immunotoxicity, and apoptosis in oocytes, which suggests the impairment of reproductive functions by nanopolystyrene.
Asunto(s)
Plásticos , Pez Cebra , Animales , Microplásticos , Oocitos/metabolismo , Estrés Oxidativo , Plásticos/metabolismo , Pez Cebra/metabolismoRESUMEN
Cyanotoxins (CTX) and micro/nanoplastics (M/NP) are ubiquitously distributed in every environmental compartment. But the distribution, abundance and associated ecological risks of CTX are still poorly understood in soil system. On the other hand, M/NP could serve as vectors for persistent organic/inorganic pollutants in the natural environment through the sorption of pollutants onto them. Thus, co-occurrence of CTX and M/NP in soils suggests the sorption of CTX onto M/NP. So, major aim of this review is to understand the relevance of CTX and M/NP in soils as co-contaminants, possible interactions between them and ecological risks of CTX in terms of phytotoxicity. In this study, we comprehensively discuss different sources and fate of CTX and the sorption of CTX onto M/NP in soil system, considering the partition coefficient of different phases of soil and mass balance. Phytotoxicity of CTX, CTX mixture and co-contaminants has also been discussed with insights on the mechanism of action. This study indicates the need for the evaluation of sorption between co-contaminants, especially CTX and M/NP, and their phytotoxicity assessment using environmentally relevant concentrations.
Asunto(s)
Contaminantes Ambientales , Contaminantes del Suelo , Adsorción , Microplásticos , Plásticos , Suelo , Contaminantes del Suelo/análisis , Contaminantes del Suelo/toxicidadRESUMEN
The phenomenon of eutrophication leads to the global occurrence of algal blooms. Cyanotoxins as produced by many cyanobacterial species can lead to detrimental effects to the biome due to their stability and potential biomagnification along food webs. Therefore, understanding of the potential risks these toxins pose to the most susceptible organisms is an important prerequisite for ecological risks assessment of cyanobacteria blooms. Fishes are an important component of aquatic ecosystems that are prone to direct exposure to cyanotoxins. However, relatively few investigations have focused on measuring the toxic potentials of cyanotoxins in teleost fishes. This review comprehensively describes the major toxicological impacts (such as hepatotoxicity, neurotoxicity, immune toxicity, reproductive toxicity and cytogenotoxicity) of commonly occurring cyanotoxins in teleost fishes. The present work encompasses recent research progresses with special emphasis on the basic molecular mechanisms by which different cyanotoxins impose their toxicities in teleost fishes. The major research areas, which need to be focused on in future scientific investigations, have also been highlighted. Protein kinase inhibition, transcriptional dysregulation, disruption of redox homeostasis and the induction of apoptotic pathways appear to be the key drivers of the toxicological effects of cyanotoxins in fish. Analyses also showed that the impacts of cyanotoxins on specific reproductive processes are relatively less described in teleosts in comparison to mammalian systems. In fact, as compared to other toxicological effects of cyanotoxins, their reproductive toxicity (such as impacts on oocyte development, maturation and their hormonal regulation) is poorly understood in fish, and thus requires further studies. Furthermore, additonal studies characterizing the molecular mechanisms responsible for the cellular uptake of cyanotoxins need to be investigated.
Asunto(s)
Cianobacterias , Contaminantes Químicos del Agua , Animales , Ecosistema , Eutrofización , Peces , Microcistinas/toxicidad , Contaminantes Químicos del Agua/toxicidadRESUMEN
Interactions of plastic particles with different organic/inorganic pollutants including heavy metals impact their ecotoxicological potential, and proper understanding in this regard is important for their ecological risk assessment. However, many studies have reported the interactions between micro-/nanoplastics (MNPs) and heavy metals (HMs), but the most prevalent interactive forces and factors monitoring their interactions are still not clear. So, the present review represents the mechanisms of interactions with special emphasis on major interactive forces and biophysicochemical and environmental factors influencing trace element's adsorption onto the surface of MNPs. Electrostatic interaction and pore-filling mechanism can best explain the HMs adsorption to MNPs. A number of biophysicochemical factors (such as biofilm, size, crystallinity, and surface charge) and environmental factors (such as pH, salt, and temperature) act together for mediating interactions and ecotoxicities of MNPs and HMs in the real environment. From a toxicological point of view, the synergistic mode of action may be more active in animals, whereas the antagonistic activity may be prevalent in plants. Besides polymer density, biofilm formation and agglomeration property of MNPs can control the vertical distribution of MNPs along the water column. Finally, the ecotoxicological potential of MNPs in the natural environment can be considered as a function of spatiotemporal variation in abiotic (including MNPs and heavy metals) and biotic components. This review will be helpful in the detail understanding of ecotoxicological risk assessment of MNPs in relation to their interaction with heavy metals.
Asunto(s)
Contaminantes Ambientales , Metales Pesados , Contaminantes Químicos del Agua , Adsorción , Animales , Metales Pesados/toxicidad , Plásticos , Agua , Contaminantes Químicos del Agua/análisisRESUMEN
Conventional chemical approaches for synthesizing nanoparticles (NPs) may restrict their applicability as they are not eco-friendly, energetically efficient and often involve toxic reducing/capping agents; but phytonanotechnology enabled the synthesis of safe, inexpensive, highly biocompatible NPs. In this regard, thorough understanding of green components and the modulatory effects of different reaction conditions on the physicochemical parameters of green synthesized NPs would be a prerequisite, which is not depicted elsewhere. This review critically analyzes the relevant reaction conditions from their mechanistic viewpoints in plant-based synthesis of NPs arising fundamental issues which need to be determined carefully. The size, stability and surface chemistry of phytogenic NPs may be fabricated as a function of multiple interconnected reaction parameters and the plant species used. The therapeutic potential of phytogenic NPs may depend on the plant species used; and so the meticulous understanding of physicochemical parameters and the family wise shorting of elite plant species may potentially benefit the theranostic future of plant-based NPs.
Asunto(s)
Tecnología Química Verde/métodos , Nanopartículas/química , Nanotecnología/métodos , Fitoquímicos/química , Extractos Vegetales/químicaRESUMEN
In mammals, interleukin 6 (IL-6) has an important function during ovulation, however, the functions of IL-6 in fish have not been elucidated. In the present study, there was quantification of de novo synthesis of ovarian IL-6 and tumor necrosis factor-alpha (TNFα) in control and hCG-treated fish and results were compared with those from an in vitro study where there was evaluation of the regulatory functions of gonadotropins and TNFα of IL-6 secretions. Relatively greater concentrations of ovarian IL-6 at the post-GVBD (post-germinal vesicle breakdown) stage indicates IL-6 modulates ovulatory processes. The hCG-induced increase in relative abundance of IL-6 (in vitro) mRNA transcript and secretion from the ovary were attenuated when there was administration of the inhibitor of TNFα secreting enzyme, TAPI-I, which indicates TNFα modulates IL-6 secretion. Treatments with IL-6 induced a marked increase in ovulation rate in vitro when there was induction of activating matrix metalloproteinase (MMP). Furthermore, treatment with IL-6 resulted in production of prostaglandin as indicated by the IL-6 induced increase in the abundance of ptgs2 mRNA transcript in the ovary of Anabas testudineus. Furthermore, results indicate the source of IL-6 in the ovary is the granulosa cells with secretion of IL-6 being induced by the additions of hCG and TNFα in the medium. There was also an IL-6-induced increase in abundance of receptors (IL-6 Rα and gp130) to which it binds indicating IL-6 autoregulates this population of receptors. Results from this study, for the first time, elucidate the reproductive functions of IL-6 in a teleost fish.
Asunto(s)
Interleucina-6/fisiología , Ovulación/fisiología , Percas/fisiología , Animales , Estradiol/farmacología , Femenino , Células de la Granulosa/efectos de los fármacos , Células de la Granulosa/fisiología , Interleucina-6/sangre , Interleucina-6/genética , Interleucina-6/farmacología , Oogénesis/efectos de los fármacos , Oogénesis/fisiología , Folículo Ovárico/efectos de los fármacos , Folículo Ovárico/fisiología , Ovulación/efectos de los fármacos , Ovulación/genética , Percas/genética , ARN Mensajero/efectos de los fármacos , ARN Mensajero/genética , ARN Mensajero/metabolismo , Proteínas Recombinantes/farmacología , Testosterona/farmacología , Factor de Necrosis Tumoral alfa/sangreRESUMEN
Plastic pollution represents a global concern for the biodiversity conservation, ecosystem and public health. The polystyrene is one of the dominant pollutants in both terrestrial and aquatic ecosystem. This work measured the hazardous nature of 100â¯nm micropolystyrene (MPS) using 25, 50, 100, 200, and 400â¯mg/L concentrations in terms of oxidative stress, morphotoxicity and cytogenotoxicity in Allium cepa. The results were compared with the positive control (PC) (400â¯mg/L chlorpyrifos). MPS significantly (pâ¯<â¯0.05) reduced the root length while induced the production of hydroxyl, superoxide radicals with a concomitant increase in DPPH scavenging activity and lipid peroxidation as compared to the negative control. The significant decrease in mitotic index with respect to the negative control (MI: 23.855⯱â¯5.336 %; lowest MI: 3.88⯱â¯1.042 %) showed the cytotoxic nature of MPS. Genotoxicity was assessed by various chromosomal and nuclear aberrations. The highest 3.029⯱â¯0.403 % (PC: 3.09⯱â¯0.535 %) chromosomal abnormality index and 2.31⯱â¯0.338 % (PC: 1.178⯱â¯0.095 %) nuclear abnormality index were observed. MPS down-regulated the expression of plant CDKA encoding gene: cdc2, an important cell cycle regulator. The overall results indicated that MPS could induce cytogenotoxicity through the exacerbation of ROS production and inhibition of cdc2.
Asunto(s)
Daño del ADN/efectos de los fármacos , Microplásticos/toxicidad , Mutágenos/toxicidad , Cebollas/efectos de los fármacos , Poliestirenos/toxicidad , Proteína Quinasa CDC2/genética , Cromosomas/efectos de los fármacos , Expresión Génica/efectos de los fármacos , Índice Mitótico , Raíces de Plantas/efectos de los fármacos , Especies Reactivas de Oxígeno/metabolismoRESUMEN
Eggplant or brinjal (Solanum melongena L.) is widely consumed worldwide and thought to trigger allergic reactions in sensitive individuals. So far, no molecular information is available on the allergy-eliciting components of eggplant. In this study, a 17â¯kDa profilin, Sola m 1, was identified from eggplant by employing an immunoproteomic approach. Based on MALDI-TOF/TOF derived sequences, the full-length cDNA of Sola m 1 was PCR amplified and then cloned. Recombinant (r) Sola m 1 was expressed in E. coli and then purified by metal affinity and gel filtration. rSola m 1 reacted with IgE-antibodies in the sera from all eggplant allergic patients. rSola m 1 also displayed allergenic activity by stimulating histamine release. rSola m 1 was monomeric, and the CD spectra revealed it to be folded with a mixture of α-helices and ß-strands. In the melting curve, rSola m 1 exhibited an irreversible denaturation where no refolding took place. Sola m 1 was found to share >80 % sequence identity with Bet v 2, which was further validated by confirming the presence of significant cross-reactivity with Bet v 2 in IgE-inhibition assay. IgE-cross reactivity was also observed between rSola m 1 and profilins from six other foods. In SGF assay, no rSola m 1-derived fragments exhibited IgE-reactivity after prolonged digestion suggesting the association of rSola m 1 with the oral allergy syndromes. Immunofluorescence localization revealed a high abundance of Sola m 1 allergen in eggplant seeds as compared to other edible parts. Taken together, Sola m 1 is the first major eggplant allergen reported in this study, which has the potential of being used as a candidate antigen in component-resolved diagnosis and immunotherapy.