A First Record of Organochlorine Pesticides in Barn Owls (Tyto alba) from Portugal: Assessing Trends from Variation in Feather and Liver Concentrations.

We evaluated feathers as a non-destructive biomonitoring tool documenting organochlorine pesticides (OCP) in liver and checked possible trends in pesticide use in two areas based on OCP concentrations in barn owls (Tyto alba). We measured the concentrations of 16 OCP in 15 primary feathers and 15 livers from barn owl carcasses collected on roadsides in Tagus Valley and Évora regions, south Portugal. Total OCP mean concentration was 8 120 ng g-1 in feathers and 178 ng g-1 in livers. All compounds were detected in feathers while in livers δ-HCH, endosulfan sulphate, p,p'-DDT and p,p'-DDD were not detected. The high ß-HCH and heptachlor concentrations in feathers most likely derived from external endogenous contamination. P,p'-DDE was the OCP with the highest hepatic concentration. Both matrices indicated an exposure to recently released heptachlor. The differing OCP concentrations between Tagus Valley and Évora seem to reflect differences in land-use and pesticide use histories of the two locations, and/or faster degradation of OCP in the Tagus area.

High Levels of Heavy Metals detected in Feathers of an Avian Scavenger Warn of a High Pollution Risk in the Atacama Desert (Chile).

The Atacama Desert represents the highest levels of mining exploitation in Chile, which inexorably results in high levels of pollution. Raptors, and particularly scavengers, have shown their usefulness to evaluate exposure to environmental contaminants in polluted scenarios. In this study, we used primary feathers from a local avian scavenger, turkey vulture Cathartes aura, to evaluate the exposure to cadmium (Cd), lead (Pb), copper (Cu) and zinc (Zn) in two locations from the southern Atacama Desert (coastal and inland) and a third location from the bordering semiarid region, in northern Chile. All metals were detected in all analyzed samples (n = 54). Mean concentrations in Coastal Atacama were 0.68 ± 0.84 ppm for Cd; 1.97 ± 2.01 ppm for Pb; 59.11 ± 80.69 ppm for Cu; and 107.96 ± 51.00 ppm for Zn, while mean concentrations in Inland Atacama were 0.55 ± 0.42 ppm for Cd; 3.37 ± 2.61 ppm for Pb; 91.66 ± 77.74 ppm for Cu; and 214.03 ± 99.08 ppm for Zn. Mean concentrations in Coastal Coquimbo were 0.63 ± 0.69 ppm for Cd; 1.57 ± 0.92 ppm for Pb; 18.09 ± 6.12 ppm for Cu; and 149.37 ± 105.56 ppm for Zn. These differences could be explained by differences on abundance of mining settlements among areas. According to the literature, these values are very high for all metals, exceeding in some cases those values referred as responsible of health disorders in birds. We strongly recommend further research looking at potential adverse effects caused by heavy metal pollution on the health of human and wildlife populations in the southern Atacama Desert.

Total Arsenic Concentrations in Sea Turtle Tissues from the Mediterranean Coast of Spain.

In this work we studied total arsenic concentrations in liver, muscle and kidney of 49 individuals of two sea turtle species (loggerhead sea turtles, n = 45; leatherback turtles, n = 4) stranded in Murcia (South-eastern Spain) coastline between 2009 and 2018. In accordance with the literature, muscle was the tissue with the highest concentrations in both species, followed by liver and kidney. Although differences in arsenic concentrations were not statistically significant between the study species, loggerhead sea turtles showed concentrations two or three times higher than those of leatherback turtles, which we attribute to differences on feeding behavior and habitat preferences. Arsenic concentrations in turtles from this area increase evidence of western Mediterranean Sea as a hotspot for metal pollution. Based on the scarce existing knowledge on arsenic toxicity in sea turtles, those levels found in our study are below those responsible for liver damage.

Physical inactivity and excessive sucrose consumption are associated with higher serum lipids in subjects with Taq1B CETP polymorphism.

BACKGROUND: Dyslipidaemias result from the interaction between genetic and environmental factors, including diet disequilibrium and physical inactivity. Among the genetic factors associated with serum lipids, the Taq1B CETP polymorphism has been investigated. The B1 allele has been considered as a risk factor for dyslipidaemia because of its association with greater CETP levels and higher serum triglycerides. The present study aimed to determine the role of the Taq1B polymorphism with lipid and anthropometric variables and its interaction with diet and physical activity. METHODS: In total, 215 subjects were enrolled in this cross-sectional study. Diet intake was evaluated using a 3-day food consumption record and physical activity was determined in accordance with World Health Organization recommendations. The Taq1B CETP polymorphism was determined by allelic discrimination. RESULTS: Subjects with the B1B2/B2B2 genotype, who had a sucrose consumption ≥5% of the total kcal day-1 , had higher levels of total cholesterol (TC) [165.55 (142.21-188.89) mg dL-1 versus 200.19 (184.79-215.60) mg dL-1 ; P for interaction = 0.034] and low-density lipoprotein [99.29 (75.52-123.05) mg dL-1 versus 128.64 (113.59-143.69) mg dL-1 ; P for interaction = 0.037] than subjects with the B1B1 genotype. Subjects who did not perform physical activity and had the B1B2/B2B2 genotype showed significantly higher levels of TC [177.48 (161.36-193.60) mg dL-1 versus 194.49 (185.43-203.56) mg mL-1 ; P for interaction = 0.033] than subjects with the B1B1 genotype. CONCLUSIONS: We provide evidence that subjects with inadequate environmental factors carriers of the polymorphic genotype had higher serum lipid levels than subjects with the B1B1 genotype.

From Mexico to the Beagle Channel: A review of metal and metalloid pollution studies on wildlife species in Latin America.

Emissions of metals and metalloids (Hg; Cd; Cr; Cu; Pb; Ni; Zn; Fe; Mn; As; Se) generated by natural (e.g., geothermal activity) or anthropic causes (eg., industry or mining) represent a worldwide contamination problem, especially in developing countries. Exposure to high concentrations of these elements is harmful to living beings, including humans. Information on this type of contamination is scarce and fragmented, limiting research which could benefit from these data. To know the state of the research, we reviewed the studies of environmental pollution by metals and metalloids carried out on animal species in Latin America. The use of animals as biomonitors of contamination by metals and metalloids is a continuously expanding practice that allows for early detection of problems. With this work, we were able to identify the most studied areas in Latin America (Amazon, Gulf of California, coastal area between Rio de Janeiro and Florianopolis and River Plate Estuary). Moreover, we provide information on the most studied metals (Hg, Cd, Cu, Pb, Zn) and wild species, which evidence the use of endangered species. The data reviewed should help researchers to direct their efforts towards sparsely researched areas and facilitate bibliographic consultation of scientific information on exposure to metals and metalloids in Latin America.

Fatty acids, epigenetic mechanisms and chronic diseases: a systematic review.

BACKGROUND: Chronic illnesses like obesity, type 2 diabetes (T2D) and cardiovascular diseases, are worldwide major causes of morbidity and mortality. These pathological conditions involve interactions between environmental, genetic, and epigenetic factors. Recent advances in nutriepigenomics are contributing to clarify the role of some nutritional factors, including dietary fatty acids in gene expression regulation. This systematic review assesses currently available information concerning the role of the different fatty acids on epigenetic mechanisms that affect the development of chronic diseases or induce protective effects on metabolic alterations. METHODS: A targeted search was conducted in the PubMed/Medline databases using the keywords "fatty acids and epigenetic". The data were analyzed according to the PRISMA-P guidelines. RESULTS: Consumption fatty acids like n-3 PUFA: EPA and DHA, and MUFA: oleic and palmitoleic acid was associated with an improvement of metabolic alterations. On the other hand, fatty acids that have been associated with the presence or development of obesity, T2D, pro-inflammatory profile, atherosclerosis and IR were n-6 PUFA, saturated fatty acids (stearic and palmitic), and trans fatty acids (elaidic), have been also linked with epigenetic changes. CONCLUSIONS: Fatty acids can regulate gene expression by modifying epigenetic mechanisms and consequently result in positive or negative impacts on metabolic outcomes.

Hg and Se in Organs of Three Cetacean Species from the Murcia Coastline (Mediterranean Sea).

We determinated Hg and Se concentrations in liver, kidney, brain, lung and muscle of five bottlenose dolphin (Tursiops truncatus), four common dolphins (Delphinus delphis) and four Risso's dolphin (Grampus griseus) stranded along the Murcia coast, Southeast Spain, in order to evaluate the risk of Hg toxicity. Hg concentrations showed similar concentrations to other individuals in the Mediterranean Sea with the same length in the same period. We observed a positive correlation of Hg and Se in liver (r = 0.948, p < 0.001) and kidney (r = 0.939; p = 0.001) and ratio the Se/Hg molar was higher than 1 in most cases. Our results suggest that the protective effects of Se against Hg toxicity occur in cetaceans. However, we detected levels of Hg described as responsible liver damage and neurotoxicological effects so other tools, as biochemical markers, should be included. Besides, more studies are needed to evaluate the risk of Hg exposure in dolphins from Murcia coastline.

Comparison of the inflammatory and stress response between sprint interval swimming and running.

The aim of the study was to compare myocellular damage, metabolic stress, and inflammatory responses as well as circulating sodium (Na+ ) and potassium (K+ ) between a single sprint swimming and running training. Eighteen subjects regularly involved in swimming and running training for at least 2 years were recruited. The subjects performed 8 × 30 seconds "all out" exercise on different days either by running or by swimming in a random order. Blood was collected before each training session, after the cessation of exercise (post) and after 2 hours of rest (2 hours). We then analyzed tumor necrosis factor alpha (TNF-α), interleukin 10 (IL-10), interleukin 6 (IL-6), cortisol, creatine kinase MB isoform (CK-MB), lactate dehydrogenase (LDH), K+ , and Na+ . Neither TNF-α nor IL-10 differed between swimming and running. Most of the subjects showed a non-statistically significant increase of LDH and CK-MB after swimming. On the other hand, IL-6 (P < .05) and cortisol (P < .05) were significantly lower after 2 hours of swimming than after running. In addition, post-exercise K+ was significantly lower (P < .001) for swimming than for running. Our results provide evidence of similar inflammatory responses between exercise modes but lower metabolic stress in response to swimming than in response to running.

Influence of a Former Mining Area in the Heavy Metals Concentrations in Blood of Free-Living Mediterranean Pond Turtles (Mauremys leprosa).

The objective of this study was to assess metal exposure in the Mediterranean Pond Turtle (Mauremys leprosa) inhabiting a watercourse in an ancient mining district polluted by different metals ("Rambla de Las Moreras", southeastern Spain) and included in the Ramsar Convention. For this purpose, mercury (Hg), lead (Pb), copper (Cu), zinc (Zn) and cadmium (Cd) were analysed in blood samples from 42 M. leprosa healthy adults (27 males and 13 females). The highest median concentrations were found for Zn, followed by Cu, Pb, Hg and Cd (366, 33, 9, 0.83 and 0.14 µg/dL, respectively). Although the literature regarding toxic metals in freshwater turtles is relatively scarce, Pb may cause deleterious effects in our population. In general, males presented higher levels than females, which could be due to maternal transfer during egg formation. The significant correlations between Cu-Cd and Cu-Hg suggest the implication of an efficient mechanism of detoxification involving metallothioneins.

Tracking pan-continental trends in environmental contamination using sentinel raptors-what types of samples should we use?

Biomonitoring using birds of prey as sentinel species has been mooted as a way to evaluate the success of European Union directives that are designed to protect people and the environment across Europe from industrial contaminants and pesticides. No such pan-European evaluation currently exists. Coordination of such large scale monitoring would require harmonisation across multiple countries of the types of samples collected and analysed-matrices vary in the ease with which they can be collected and the information they provide. We report the first ever pan-European assessment of which raptor samples are collected across Europe and review their suitability for biomonitoring. Currently, some 182 monitoring programmes across 33 European countries collect a variety of raptor samples, and we discuss the relative merits of each for monitoring current priority and emerging compounds. Of the matrices collected, blood and liver are used most extensively for quantifying trends in recent and longer-term contaminant exposure, respectively. These matrices are potentially the most effective for pan-European biomonitoring but are not so widely and frequently collected as others. We found that failed eggs and feathers are the most widely collected samples. Because of this ubiquity, they may provide the best opportunities for widescale biomonitoring, although neither is suitable for all compounds. We advocate piloting pan-European monitoring of selected priority compounds using these matrices and developing read-across approaches to accommodate any effects that trophic pathway and species differences in accumulation may have on our ability to track environmental trends in contaminants.

Contaminants in the southern tip of South America: Analysis of organochlorine compounds in feathers of avian scavengers from Argentinean Patagonia.

The aim of this study was to assess the exposure to organochlorine compounds (OC) in 91 primary wing feathers of avian scavengers, Turkey vulture (Cathartes aura), American black vulture (Coragyps atratus) and Southern crested caracaras (Polyborus plancus) from the southern tip of South America, in the Argentinean Patagonia. We analyzed for a series of OC including hexachlorocyclohexane (HCH) isomers, endosulfan, aldrin, dieldrin, endrin, dichlorodiphenyltrichloroethane (p,p'-DDT), dichlorodiphenyldichloroethane (p,p'-DDD), dichlorodiphenyldichloroethylene (p,p'-DDE), heptachlor and heptachlor-epoxide. This is the first study on OC in feathers of three terrestrial top carnivores from South America. OC concentrations found in the studied species were much higher than those found in feathers of raptors from Europe and Asia, which likely indicate their high use in the region, specifically in agriculture, and other possible uses of OC in this area. ∑HCH had the highest median concentration, followed by ∑Drins, ∑DDT, ∑Heptachlor, and ∑Endosulfan, similar to those reported in several food samples in Argentina. On the other hand, differences in OC profiles between species and areas may be related to feeding and migratory habits, as well as the molt period. Three individuals showed ∑DDT (DDT, DDD and DDE) concentrations in feathers related to sublethal effects. However, this comparison should be used with caution due to problems with extrapolating such data across tissues and species.

Oral quercetin supplementation hampers skeletal muscle adaptations in response to exercise training.

We aimed to test exercise-induced adaptations on skeletal muscle when quercetin is supplemented. Four groups of rats were tested: quercetin sedentary, quercetin exercised, placebo sedentary, and placebo exercised. Treadmill exercise training took place 5 days a week for 6 weeks. Quercetin groups were supplemented with quercetin, via gavage, on alternate days throughout the experimental period. Sirtuin 1 (SIRT1), peroxisome proliferator-activated receptor γ coactivator-1α mRNA levels, mitochondrial DNA (mtDNA) content, and citrate synthase (CS) activity were measured on quadriceps muscle. Redox status was also quantified by measuring muscle antioxidant enzymatic activity and oxidative damage product, such as protein carbonyl content (PCC). Quercetin supplementation increased oxidative damage in both exercised and sedentary rats by inducing higher amounts of PCC (P < 0.001). Quercetin supplementation caused higher catalase (P < 0.001) and superoxide dismutase (P < 0.05) activity in the non-exercised animals, but not when quercetin is supplemented during exercise. Quercetin supplementation increased SIRT1 expression, but when quercetin is supplemented during exercise, this effect is abolished (P < 0.001). The combination of exercise and quercetin supplementation caused lower (P < 0.05) mtDNA content and CS activity when compared with exercise alone. Quercetin supplementation during exercise provides a disadvantage to exercise-induced muscle adaptations.

Quercetin effects on weight gain and caloric intake in exercised rats.

Quercetin is a flavonoid which activates oxidative metabolism. Quercetin may reduce weight gain by decreasing feed efficiency. The present study aims to evaluate weight gain, caloric intake and feed efficiency in exercised and sedentary rats supplemented with quercetin. Wistar rats were divided into four groups: quercetin-exercise training (QT), quercetin-sedentary (QS), placebo-exercise training (PT) and placebo-sedentary (PS). Rats were exercised and/or orally supplemented with quercetin (25 mg · kg(-1) on alternate days) during six weeks. Weight gain of the QT group decreased when compared with the PT and PS groups. Exercised groups increased cumulative caloric intake during the experimental period. The QT group rats also reduced their feed efficiency when compared with the QS and PS groups. These results suggest that quercetin is not able to decrease weight gain because no differences were found between placebo and quercetin condition either in the sedentary or in the training condition.

Predictive value of stabilometry and fear of falling on falls in postmenopausal women.

OBJECTIVES: Falls are one of the leading causes of fractures and impaired quality of life in the elderly, and they are related to balance deficit and to fear of falls. The purpose of our study is to evaluate predictors of falls in the 50-65-year-old postmenopausal population. METHODS: A prospective cohort study was conducted on 96 postmenopausal women. Fear of falling and postural stability were assessed by using the FES-I (Falls Efficacy Scale-International) and a force platform, respectively. Fall frequency was determined in the 12-month follow-up study period. Multivariate logistic regression was used to identify predictive factors of falls. RESULTS: Fear of falls, the FES-I scale and four stabilometric parameters, specifically under eyes-closed condition, were significantly higher in the group of fallers. The root mean square amplitude in the medial-lateral direction with eyes closed (RMSXec) (odds ratio 5.1, 95% confidence interval (CI) 1.6-15.5, p = 0.004) and FES-I (odds ratio 3.4, 95% CI 1.1-10.5, p = 0.026) were the best independent predictive factors of the risk of falling. CONCLUSIONS: RMSXec > 0.133 was the best predictive factor for falls in our group of 50-65-year-old postmenopausal women studied, and a FES-I score > 20 could predict falls in this population.

Effect of combined electrostimulation and plyometric training on 30 meters dash and triple jump.

AIM: The aim of this paper was to analyze the effects of training combining plyometrics (PT) and neuromuscular electrostimulation (ES) on speed training and triple jump. The study consisted on the application of an electrostimulation protocol and plyometric jumps to four groups of young athletes (Control, G II, G III and G IV). METHODS: Eighty-four young athletes took part in the study (40 girls and 44 boys). All of them were sprinters (100 and 200 meters dash, and 100 and 110 hurdles meters), their mean age, weight and height being 15.9±1.4 years old, 58.53±8.05 kg, and 1.68±0.07 m, respectively. After 8 weeks of training, a 30-meter sprint launched test -time being measured by photoelectric cells - and a triple jump test from static position were completed. Repeated measures ANCOVA were used. RESULTS: The only group that improved significantly in the speed test (P<0.001) relative to the control group was G IV. In the triple jump test, improvements were significant, (P<0.05) and (P<0.01), in G II and G IV, respectively, relative to the control group. The results of ES + PT combined training offered no significant differences in either speed test and triple jump by gender. CONCLUSION: The most effective training aimed at improving the speed of 30 m is simultaneous combined training. Regarding triple jump, the results showed significant improvements in the performance of athletes who used both simultaneous combined training and used ES followed by plyometrics. However, no significant improvement was observed after PT training prior to ES.

Assessment of polycyclic aromatic hydrocarbons (PAHs) in mediterranean top marine predators stranded in SE Spain.

Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous environmental pollutants. Although they are not bioaccumulated in vertebrates, chronic exposures might still derive on serious toxic effects. We studied concentrations of 16 reference PAHs on blubber of two dolphin species (striped dolphin, n = 34; and bottlenose dolphin, n = 8) and one marine turtle (loggerhead turtle, n = 23) from the Mediterranean waters of SE Spain, an important or potential breeding area for these and other related species. Σ16 PAHs concentrations were relatively similar between the three species, but they were in the lower range in comparison to worldwide data. Of the six PAHs detected, fluoranthene was the only high molecular weight (HMW) PAH, so low molecular weight (LMW) PAHs predominated. Naphthalene and phenanthrene were invariably those PAHs with higher detection rates as well as those with higher concentrations. In accordance with the literature, sex and length did not have significant influence on PAHs concentrations, probably due to high metabolization rates which prevent for observation of such patterns. Despite LMW PAHs are considered less toxic, we cannot dismiss toxic effects. This is the first work assessing PAHs concentrations in cetaceans and sea turtles from the SE Spain, which could serve as the baseline for future research.

POPs concentrations in cetaceans stranded along the agricultural coastline of SE Spain show lower burdens of industrial pollutants in comparison to other Mediterranean cetaceans.

Despite the Mediterranean Sea being one of the world's marine biodiversity hotspots, it is a hotspot of various environmental pollutants. This sea holds eight cetacean with resident populations whose numbers are considered to decline in the last decades and which are particularly susceptible to POPs bioaccumulation due to their peculiar characteristics. In this work, we studied blubber concentration of various OCPs and several PCBs and PBDEs congeners in cetaceans stranded in the northern coast of the Gulf of Vera (Region of Murcia, SE Spain) between 2011 and 2018. Most compounds and congeners were above the limit of detection in most samples, although some pesticides like endosulfan stereoisomers or endrin were never detected. DDT and its metabolites, PCBs and metoxychlor appear as the dominant compounds while PBDEs shows concentrations of lower magnitude. Striped dolphin was the species accounting for higher concentrations of most pollutants. There were differences in concentrations and profiles between species which could be partially explained by differences on diet and feeding behavior. We also observed differences based on life history parameters suggesting maternal transfer for most POPs, in accordance with other works. DDE/ ΣDDT ratio suggest no recent exposure to these pesticides. Despite showing lower concentrations than some previous works, PCB concentrations accounted for higher total TEQ than many studies. According to toxicity thresholds in the literature, we cannot guarantee the absence of health consequences on populations studied, especially for those caused by PCBs. These findings are of major importance considering the relevance of the study area in the conservation of Mediterranean cetaceans.

Trace elements in blood of Baltic gray seal pups (Halichoerus grypus) from the Gulf of Riga and their relationship with biochemical and clinical parameters.

Trace elements are pollutants of both natural and anthropogenic origin which can influence negatively on ecosystem and wildlife health. We evaluated trace element in blood samples of gray seal (Halichoerus grypus) stranded in the Gulf of Riga and their influence on their health status through hematological and biochemical profiles. Zn showed the highest levels followed by Cu > Se > Pb > THg > As. Cr and Cd were not detected. Most trace element levels were generally comparable to those reported in seal species; however, high Pb values were observed in those sample showing detectable concentrations (<0.046-257.6 µg/kg ww). Significant positive correlations were found between trace elements concentrations and various biochemical parameters, including Se-ASAT, Se:Hg-ASAT, Cu-TP, Cu-ALB, CuCa, Zn-ALAT, ZN-LDH, ZnP, Zn-Segment neutrophils, and Pb-CK. Nevertheless, most relationships were not strong enough (p > 0.04) to assume a toxicological implication. Despite its limitations, this information could serve as the baseline for future research.

Blood lead levels and δ-ALAD inhibition in nestlings of Eurasian Eagle Owl (Bubo bubo) to assess lead exposure associated to an abandoned mining area.

In order to biomonitor lead contamination in Southeastern Spain, 218 blood samples from 28 to 30-day old Eurasian Eagle Owl chicks (Bubo bubo) born between 2003 and 2007 were analysed. In general, mean lead levels showed that chicks were exposed to background concentrations. However, mean levels in chicks born in an ancient and abandoned mining site ("Sierra Minera Cartagena-La Union") or in their surroundings (Geometric mean (GM) = 5.83 µg/dl, range 0.49-25.61 µg/dl), an area highly polluted by lead and other metals, were significantly higher (p < 0.001) than the rest of the population (GM = 1.66 µg/dl, range = Non detected-18.37 µg/dl). Because δ-ALAD activity is considered the best biomarker for lead exposure and effect in birds, the activity of this enzyme was also evaluated and correlated with lead levels in blood. In this study, low levels of blood lead inhibited δ-ALAD, even when lead concentrations were lower than the limits described by other authors in raptors. Adverse effects caused by this inhibition may occur when blood lead levels were above 15 µg/dl, although only eight chicks presented these concentrations in their blood. Sampling site also influenced enzymatic activity, since it decreased about 60% in the polluted area in relation to the rest. For all these reasons, further research regarding risk assessment for lead exposure in Eagle Owls nesting in the polluted area is advisable. Our results suggest that the Eurasian Eagle Owl can be considered a suitable sentinel animal for monitoring lead contamination and δ-ALAD activity can be used as a sensitive biomarker for lead exposure and effect in this species.

Blood delta-ALAD, lead and cadmium concentrations in spur-thighed tortoises (Testudo graeca) from Southeastern Spain and Northern Africa.

Mediterranean spur-thighed tortoise (Testudo graeca) is actually included in the IUCN as vulnerable species. Its main European population is located in southeastern Spain. Although a great deal of information has been acquired on the internal medicine and survey and even parasitological fauna on these animals, there are no references about contaminants levels in this species. The objectives of this study were to compare the levels of two metals (cadmium and lead) in the blood of spur-thighed tortoises from two different populations, one from Southeastern of Spain (n = 22) and the other from North of Africa (n = 39), kept in captivity at the Santa Faz Recuperation Centre (Alicante, Spain) and to investigate the relationship between their blood levels of lead and their blood delta-aminolevulinic acid dehydratase (delta-ALAD) activity. Blood lead and cadmium concentrations were higher in tortoises from African than in those from Spain. Moreover, a negative and significant correlation (P < 0.05) was found between delta-ALAD activity and blood lead levels, indicating the suitability of this enzyme as biomarker for lead in this species.