Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 120
Filtrar
Más filtros

Banco de datos
País/Región como asunto
Tipo del documento
País de afiliación
Intervalo de año de publicación
1.
J Clin Endocrinol Metab ; 53(5): 1081-3, 1981 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-7287876

RESUMEN

The effect of chlorpromazine on serum levels of PRL was studied in two anencephalic infants without identifiable hypothalamic tissue. After a single im injection of chlorpromazine, serum concentrations of PRL rose about 3-fold. This result indicates that chlorpromazine stimulates PRL release by directly acting on the pituitary gland in humans.


Asunto(s)
Anencefalia/sangre , Clorpromazina , Prolactina/sangre , Femenino , Edad Gestacional , Humanos , Recién Nacido , Embarazo
2.
J Clin Endocrinol Metab ; 83(2): 333-8, 1998 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-9467536

RESUMEN

To evaluate the clinical utility of recently developed biochemical markers in the assessment of bone metabolism during GnRH agonist (GnRHa) treatment, we compared five bone resorption markers [C-telopeptide (CTX) and N-telopeptide (NTX) of type I collagen, hydroxyproline (Hpr), pyridinoline (Pyr), and deoxypyridinoline (Dpyr)] and two bone formation markers [total alkaline phosphatase (Alp) and osteocalcin (OC)]. Sixty-eight normally menstruating women were injected with a long-acting GnRHa once a month for 24 weeks for the treatment of endometriosis or leiomyoma. The mean percentage bone loss at the lumbar spine was 3.79% at the end of treatment. Although levels of all markers increased significantly as the treatment progressed, CTX and NTX exhibited the highest correlation coefficients between bone loss at 24 weeks and the seven markers measured at 0, 4, 12, 16, and 24 weeks of treatment. Serum estradiol levels were similarly suppressed during the treatment in both fast losers (whose bone loss was more than the mean) and slow losers (whose bone loss was less than the mean). However, significantly higher z-scores of bone resorption markers, but not of bone formation markers, were observed in the fast losers at 24 weeks of treatment, suggesting a more accelerated bone resorption in this group. Whereas the three highest z-scores at 24 weeks of treatment were CTX, NTX, and Dpyr (in that order), the highest z-score (P < 0.05) was observed for CTX in the fast losers. The subjects in the highest quartile of CTX, the highest, and second highest quartiles of NTX at 24 weeks of treatment experienced 2.1, 2.2, and 1.7 times more bone loss (P < 0.001), respectively, than those in the lowest quartiles. Furthermore, the subjects in the highest quartile of both CTX and NTX experienced 3.6 times more bone loss (P < 0.001) than those in the lowest quartile of both markers. These results indicate that both CTX and NTX are useful and sensitive markers for bone resorption in a hypoestrogenic state induced by GnRHa.


Asunto(s)
Biomarcadores , Remodelación Ósea/efectos de los fármacos , Buserelina/farmacología , Colágeno/sangre , Goserelina/farmacología , Péptidos/sangre , Adulto , Fosfatasa Alcalina/sangre , Aminoácidos/sangre , Antineoplásicos Hormonales/uso terapéutico , Buserelina/uso terapéutico , Colágeno Tipo I , Endometriosis/tratamiento farmacológico , Estradiol/sangre , Femenino , Goserelina/uso terapéutico , Humanos , Hidroxiprolina/sangre , Leiomioma/tratamiento farmacológico , Osteocalcina/sangre , Estudios Retrospectivos
3.
J Clin Endocrinol Metab ; 81(2): 752-6, 1996 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-8636299

RESUMEN

In a longitudinal study, we analyzed the speed of sound (SOS) and broadband ultrasound attenuation (BUA) of the os calcis as an index of bone mineral density (BMD) to define the effects of pregnancy and lactation on bone metabolism. We used an ultrasound bone densitometer and measured 6 biochemical markers of bone turnover in 18 healthy women throughout pregnancy and puerperium. The measurement of SOS and BUA by such an ultrasound device was clinically advantageous; not only is it radiation-free technology, but it also correlates highly with BMD measured by conventional X-ray bone densitometry. While a significant decrease in SOS was found in the 3rd trimester of pregnancy as compared with the early stage of pregnancy, there was no difference in both SOS and BUA between the breast-feeding women and the principally formula-feeding women during a 6-month period of puerperium. The analysis of biochemical markers revealed that both bone formation and bone resorption were elevated in the 3rd trimester of pregnancy as well as during puerperium, and that the breast-feeding women had significantly higher bone metabolism than the principally formula-feeding women. These results indicate that bone mass decreases as bone turnover itself is enhanced during pregnancy, while lactation does not substantially affect bone mass during at least 6 months of puerperium, although bone turnover is active.


Asunto(s)
Densidad Ósea , Huesos/diagnóstico por imagen , Huesos/metabolismo , Periodo Posparto/fisiología , Embarazo/fisiología , Adulto , Fosfatasa Alcalina/sangre , Remodelación Ósea , Creatinina/orina , Femenino , Humanos , Hidroxiprolina/orina , Lactancia/fisiología , Estudios Longitudinales , Factores de Tiempo , Ultrasonografía
4.
J Clin Endocrinol Metab ; 79(1): 223-6, 1994 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-8027231

RESUMEN

Although there are some reports, including our previous study, indicating the existence and biological action of epidermal growth factor (EGF) in human decidua, the site of its synthesis remains unknown. To clarify the EGF production during decidualization at a molecular level, gene expression of EGF in human endometrium/decidua was examined by Northern blot analysis and in situ hybridization. Northern blot hybridization using 32P-labeled human prepro-EGF complementary DNA was carried out for nonpregnant human endometria from hysterectomized uteri of leiomyoma, decidua from early pregnancy, and in vitro decidualized endometrial cells by medroxyprogesterone acetate (MPA). Although no hybridized band was found in the proliferative and secretory phase endometria, a specific band of 5 kilobases, in agreement with the size of human prepro-EGF messenger ribonucleic acid, was detected in decidua of early pregnancy as well as in in vitro MPA-induced decidual cells. In situ hybridization revealed that prepro-EGF messenger ribonucleic acid was observed in the stromal cells of decidua. These results demonstrate that the EGF gene is expressed in the process of decidualization, suggesting that EGF may play an important role in the decidualization process of human endometrium.


Asunto(s)
Decidua/fisiología , Endometrio/metabolismo , Factor de Crecimiento Epidérmico/genética , Expresión Génica , Northern Blotting , Sondas de ADN , Endometrio/efectos de los fármacos , Femenino , Humanos , Hibridación in Situ , Leiomioma/metabolismo , Acetato de Medroxiprogesterona/farmacología , Embarazo , Precursores de Proteínas/genética , ARN Mensajero/análisis , ARN Mensajero/metabolismo , Neoplasias Uterinas/metabolismo
5.
J Clin Endocrinol Metab ; 83(2): 326-32, 1998 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-9467535

RESUMEN

We examined sequential changes of bone-resorbing cytokines and bone metabolic markers and the effect of ovarian hormones on bone metabolism during the menstrual cycle in 10 healthy Japanese women, aged 22-43 yr, with normal ovarian function. Serum soluble interleukin-6 receptor (sIL-6R) showed a significant variation; a rise during the early and late follicular periods followed by a fall during the early luteal period (P = 0.0423, P = 0.0334) and an increase during the mid and late luteal periods. There were significant changes in the levels of markers of bone formation: a rise in serum bone-specific alkaline phosphatase (ALP) during the mid and late follicular (P = 0.0265) periods and a fall in serum carboxyl-terminal propeptide of type I procollagen (PICP) during the midluteal period (P = 0.0161). As for the levels of bone resorption markers, urinary type I collagen C-telopeptide breakdown products (CTx) and free deoxypyridinoline (D-Pyr) decreased significantly during the early and midfollicular periods, urinary free D-Pyr and serum pyridinoline cross-linked carboxyl-terminal telopeptide of type I collagen (ICTP) (P = 0.0440) increased significantly during the early luteal period, and urinary CTx, free D-Pyr, and serum ICTP decreased significantly during the late luteal period (P = 0.0170-0.0008). The serum PTH level was significantly higher during the follicular than the luteal period (P = 0.0132). Serum sIL-6R significantly correlated with urinary CTx (r = 0.190, P < 0.05) and serum ALP (r = 0.209, P < 0.05) and serum estradiol with intact osteocalcin (r = 0.309, P < 0.0005) and serum ALP (r = 0.181, P < 0.05). These observations strongly suggest that cyclic variations in the levels of bone formation and resorption markers and of a bone-resorbing cytokine may be modulated by cyclic changes in serum steroid hormones during the menstrual period. In addition, the specific days of biochemical events in the menstrual cycle are crucial for evaluating osteoclastic and osteoblastic activities in pre- and perimenopausal women or in women starting GnRH agonist therapy.


Asunto(s)
Biomarcadores , Huesos/metabolismo , Ciclo Menstrual/metabolismo , Receptores de Interleucina-6/sangre , Adulto , Fosfatasa Alcalina/sangre , Aminoácidos/sangre , Remodelación Ósea , Colágeno/orina , Colágeno Tipo I , Estradiol/sangre , Femenino , Humanos , Hormona Luteinizante/sangre , Osteocalcina/sangre , Hormona Paratiroidea/sangre , Fragmentos de Péptidos/sangre , Péptidos/orina , Procolágeno/sangre , Progesterona/sangre , Solubilidad
6.
Clin Exp Metastasis ; 13(2): 89-96, 1995 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-7882618

RESUMEN

Two human ovarian adenocarcinoma cell lines, MCAS-3 and OVISE-3 were found to secrete little of any type of gelatinase in tissue culture. However, when these cell lines were implanted subcutaneously into nude mice the cyst fluids from the resultant tumors contained gelatinase A and/or B. The enzyme activities, especially of gelatinase B, were much higher in the malignant MCAS-3 tumors than in those of the less malignant OVISE-3 tumor cells. To elucidate the origin of gelatinase B in cyst fluids of the MCAS-3 tumors, murine skin fibroblasts (MSF) were isolated from a subcutaneous tumor in a nude mouse and tested for their proteinase secretion in culture. MSF cells, which secreted some gelatinase A and gelatinase B, were induced to secrete high levels of both enzymes, especially gelatinase B, by co-cultivation with MCAS-3 cells. In addition, gelatinase A activity was induced by incubation of MSF cells with the conditioned medium of either MCAS-3 or OVISE-3 cells, whereas gelatinase B was induced only with that of MCAS-3. Although cytokines or growth factors such as IL-1 beta, TGF-beta 1, TNF-alpha or EGF stimulated the secretion of gelatinases A and B from MSF cells, their effects on gelatinase B activity were far less than that of the MCAS-3 conditioned medium. These results indicate that the major part of gelatinase B activity in the cyst fluids of the ovarian tumors is secreted by host interstitial cells stimulated by tumor-derived humoral factors. Similar tumor cell-host cell interactions may be important in the production of various proteinases in other tumor types.


Asunto(s)
Colagenasas/biosíntesis , Neoplasias Ováricas/enzimología , Animales , Citocinas/farmacología , Inducción Enzimática , Femenino , Fibroblastos/enzimología , Humanos , Metaloproteinasa 9 de la Matriz , Ratones , Ratones Desnudos , Trasplante de Neoplasias , Neoplasias Ováricas/patología , Trasplante Heterólogo , Células Tumorales Cultivadas
7.
Placenta ; 19(2-3): 217-23, 1998.
Artículo en Inglés | MEDLINE | ID: mdl-9548189

RESUMEN

Placental protein 5 (PP5) is a placenta-derived glycoprotein with serine proteinase-inhibiting activity. To date its physiological functions have not been well elucidated. Recently, cDNA sequence analysis revealed that PP5 belongs to the Kunitz-type proteinase inhibitor family and it is identical to tissue factor pathway inhibitor-2 (TFPI-2), homologous to TFPI. Northern blot analysis demonstrated that placental tissue is extremely rich in the transcripts. This study localized PP5/TFPI-2 mRNA in placental tissues at three different gestational periods using in situ hybridization. PP5/TFPI-2 mRNA was specifically detected in syncytiotrophoblast at any gestational period examined, suggesting that syncytiotrophoblast is the principal production site of PP5/TFPI-2 in developing placental tissues. This mRNA expression pattern of PP5/TFPI-2 is quite different from that of TFPI, which is mainly found in vascular endothelial cells. The results indicated possible roles of PP5/TFPI-2 in the trophoblast differentiation and in the maintenance of intervillous blood flow. Also, Northern analysis demonstrated no or little expression of PP5/TFPI-2 in four choriocarcinoma cell lines, in contrast to its abundant expression in syncytiotrophoblast.


Asunto(s)
Glicoproteínas/metabolismo , Proteínas Gestacionales/metabolismo , ARN Mensajero/metabolismo , Inhibidores de Serina Proteinasa/metabolismo , Trofoblastos/metabolismo , Adulto , Northern Blotting , Coriocarcinoma/metabolismo , Femenino , Edad Gestacional , Glicoproteínas/genética , Humanos , Hibridación in Situ , Embarazo , Proteínas Gestacionales/genética , Inhibidores de Serina Proteinasa/genética , Células Tumorales Cultivadas , Neoplasias Uterinas/metabolismo
8.
Mol Cell Endocrinol ; 135(2): 165-8, 1997 Dec 12.
Artículo en Inglés | MEDLINE | ID: mdl-9484912

RESUMEN

Gonadotropin-releasing hormone (GnRH) has been reported to exist in extrahypothalamic tissues such as the placenta, gonads and mammary glands. While we have reported the presence of GnRH-mRNA in the rodent uterus, there have been no reports concerning gene expression of GnRH and its receptor (GnRH-R) in human endometrial tissue. In order to investigate the role of GnRH as a local regulator in the human endometrium, we examined the gene for GnRH and GnRH-R in non-pregnant endometrium and decidua of early pregnancy. Using reverse transcriptase-polymerase chain reaction (RT-PCR) and Southern blot analysis we found GnRH-mRNA but not GnRH-R-mRNA transcripts in the human endometrium and decidua at 7-9 weeks gestation. This is the first report that suggests GnRH gene expression in the human endometrium/decidua.


Asunto(s)
Decidua/metabolismo , Endometrio/metabolismo , Hormona Liberadora de Gonadotropina/genética , Receptores LHRH/genética , Southern Blotting , Decidua/química , Endometrio/química , Femenino , Expresión Génica/genética , Edad Gestacional , Humanos , Reacción en Cadena de la Polimerasa , Embarazo
9.
Mol Cell Endocrinol ; 132(1-2): 73-80, 1997 Sep 19.
Artículo en Inglés | MEDLINE | ID: mdl-9324048

RESUMEN

To clarify the biological significance of platelet-derived growth factor (PDGF) in human decidual cell function, which is important for the maintenance of pregnancy, we investigated gene expression of the PDGF subunits, PDGF-A and PDGF-B, specific binding of the PDGF isoform, and the effect of PDGF dimers on DNA synthesis in human decidual cells. We detected in decidua from early pregnancy the expected DNA bands of PDGF-A and PDGF-B by reverse transcriptase-polymerase chain reaction (RT-PCR) as well as mRNAs of each PDGF subunit by Northern blot hybridization, demonstrating that both PDGF subunits exist in this tissue. Scatchard plot analysis showed that decidual cells had both PDGF-alpha and PDGF-beta receptors. PDGF-AA, -AB and -BB stimulated [3H]-thymidine incorporation in cultured decidual cells in a dose-dependent manner. These results indicate the importance of PDGF in human decidua.


Asunto(s)
Decidua/metabolismo , Regulación del Desarrollo de la Expresión Génica , Factor de Crecimiento Derivado de Plaquetas/genética , Factor de Crecimiento Derivado de Plaquetas/metabolismo , Receptores del Factor de Crecimiento Derivado de Plaquetas/metabolismo , Sitios de Unión , Femenino , Humanos , Embarazo
10.
Eur J Endocrinol ; 137(2): 167-71, 1997 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-9272105

RESUMEN

OBJECTIVE: The major side effect of GnRH agonist (GnRHa) therapy is the reduction of bone mass. To analyze bone resorption by GnRHa, we measured the urinary excretion of C-terminal telopeptides of type I collagen (CTX), a new marker of bone resorption. METHODS: We used a new ELISA for CTX (CrossLaps) in a sample of 18 premenopausal women with leiomyoma who were treated with daily administration of 400 micrograms nafarelin or 900 micrograms buserelin for 16 weeks. RESULTS: Urinary CTX excretion increased significantly during GnRHa treatment and then decreased at 12 and 24 weeks after the cessation of GnRHa therapy. Whereas the excretory profile of CTX during GnRHa therapy was almost similar to that of pyridinoline (Pyr) or deoxypyridinoline (D-Pyr), both biochemical markers of bone resorption, the magnitude of the change in CTX was significantly greater than that in Pyr or D-Pyr. CONCLUSIONS: These results indicate that CTX could be a more sensitive marker for bone resorption than the currently used biochemical markers, and that CrossLaps ELISA is useful for therapeutic monitoring during and after GnRHa treatment.


Asunto(s)
Resorción Ósea/inducido químicamente , Resorción Ósea/orina , Colágeno/química , Colágeno/orina , Hormona Liberadora de Gonadotropina/agonistas , Adulto , Aminoácidos/orina , Antineoplásicos Hormonales/efectos adversos , Antineoplásicos Hormonales/uso terapéutico , Biomarcadores , Densidad Ósea/efectos de los fármacos , Buserelina/efectos adversos , Buserelina/uso terapéutico , Femenino , Hormona Liberadora de Gonadotropina/efectos adversos , Hormonas/efectos adversos , Hormonas/uso terapéutico , Humanos , Leiomioma/tratamiento farmacológico , Persona de Mediana Edad , Nafarelina/efectos adversos , Nafarelina/uso terapéutico , Neoplasias Uterinas/tratamiento farmacológico
11.
Eur J Endocrinol ; 133(6): 741-6, 1995 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-8548061

RESUMEN

In order to analyze the involvement of growth factors in the implantation mechanism, we examined the direct effects of epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha) on trophoblast outgrowth of the mouse blastocyst in vitro. ICR mouse blastocysts were cultured for 4 days on a culture plate in medium containing EGF or TGF-alpha or conditioned medium obtained from cultured endometrial epithelial cells. Blastocysts were also co-cultured with endometrial epithelial cells. The trophoblast outgrowth of these cultured blastocysts was observed daily and the percentage of outgrowing embryos was calculated and analyzed statistically by the chi-squared test. Analysis for the specific binding of 125I-EGF in outgrown trophoblasts was carried out by autoradiography. The co-culture (days 3 and 4) and the presence of EGF (10 ng/ml, day 4), TGF-alpha (1 ng/ml, day 3; 10 ng/ml, days 2 and 3; 50 ng/ml, days 2-4) or conditioned medium (days 3 and 4) significantly stimulated the rate of trophoblast outgrowth. Preincubation of the conditioned medium with monoclonal anti-EGF or anti-TGF-alpha antibody suppressed the stimulatory effect of the conditioned medium on trophoblast outgrowth. The specific 125I-EGF binding in outgrown trophoblasts was demonstrated by autoradiography. These results suggest that EGF and TGF-alpha play an important role in the implantation process by directly stimulating trophoblast development.


Asunto(s)
Factor de Crecimiento Epidérmico/farmacología , Factor de Crecimiento Transformador alfa/farmacología , Trofoblastos/citología , Trofoblastos/efectos de los fármacos , Animales , Anticuerpos Monoclonales/farmacología , Blastocisto/citología , Blastocisto/efectos de los fármacos , Blastocisto/fisiología , División Celular/efectos de los fármacos , División Celular/fisiología , Células Cultivadas , Técnicas de Cocultivo , Medios de Cultivo Condicionados/farmacología , Endometrio/citología , Endometrio/efectos de los fármacos , Endometrio/fisiología , Factor de Crecimiento Epidérmico/inmunología , Células Epiteliales , Epitelio/efectos de los fármacos , Epitelio/fisiología , Femenino , Radioisótopos de Yodo , Masculino , Ratones , Ratones Endogámicos ICR , Embarazo , Albúmina Sérica Bovina/farmacología , Factor de Crecimiento Transformador alfa/inmunología , Trofoblastos/fisiología
12.
Eur J Endocrinol ; 132(2): 200-5, 1995 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-7858739

RESUMEN

In order to investigate the biological significance of epidermal growth factor (EGF) in the cell function of teratocarcinoma cells, we examined the production, binding and cell proliferative effect of EGF in PA-1 human ovarian teratocarcinoma cell line. The immunoreactivity of EGF in PA-1 cell-conditioned medium was detected by human EGF radioimmunoassay, and prepro-EGF mRNA was demonstrated in PA-1 cells by Northern blot analysis. An [125I]EGF binding study showed the presence of EGF receptor with very high binding affinity and relatively low numbers of binding sites in PA-1 cells. Furthermore, the growth of PA-1 cells was stimulated by EGF and inhibited by anti-EGF monoclonal antibody. These results suggest strongly that EGF plays an important role in controlling the growth of teratocarcinoma cells as an autocrine/paracrine growth factor.


Asunto(s)
División Celular/efectos de los fármacos , Factor de Crecimiento Epidérmico/farmacología , Neoplasias Ováricas/patología , Teratocarcinoma/patología , Northern Blotting , Línea Celular , Medios de Cultivo Condicionados , Factor de Crecimiento Epidérmico/biosíntesis , Femenino , Humanos , ARN Mensajero/análisis , Ensayo de Unión Radioligante
13.
Keio J Med ; 41(3): 154-60, 1992 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-1434310

RESUMEN

The objective of this study was to reveal the health effects of acrylonitrile (AN) in seven Japanese acrylic fiber manufacturing factories. The study subjects were 157 AN-exposed male shift workers who had been exposed to AN for 17 years on the average and 537 control workers whose working conditions were similar to those of the AN-exposed workers. The seven factories were classified into two groups according to their AN exposure levels in 1987, and also into three groups on the basis of 1976 exposure levels. The most highly exposed group of subjects showed a mean AN concentration of 1.13 ppm by personal sampling. Medical examination failed to detect any health effects attributable to long-term exposure to AN, although the existence of some symptoms of acute irritation could not be completely ruled out. The results of this study may provide a "no observed effects level" for human on-the-job exposure with regard to the health effects examined here.


Asunto(s)
Acrilonitrilo/efectos adversos , Tamizaje Masivo , Exposición Profesional , Estudios Transversales , Humanos
14.
Brain Res ; 820(1-2): 71-6, 1999 Feb 27.
Artículo en Inglés | MEDLINE | ID: mdl-10023032

RESUMEN

We investigated the effect of glucose and its related substrates on the recovery of pulsatile luteinizing hormone (LH) secretion which was suppressed by insulin in estrogen-primed ovariectomized rats. We also examined the effect of glucose on the electrical activity of the gonadotropin-releasing hormone (GnRH) pulse generator which was suppressed by insulin. The intravenous (i.v.) injection of insulin (5 units/rat) suppressed the pulsatile LH secretion for 3 h in estrogen-primed ovariectomized rats. This suppressive effect of insulin on the LH secretion was rapidly reversed by the i.v. injection of glucose and mannose but not by the injection of lactate and saline. Fructose could recover the LH secretion suppressed by insulin, but took a longer time than glucose did. By monitoring the electrical activity of the GnRH pulse generator, we found that i.v. injection of insulin suppressed the pulsatile LH secretion by decreasing the activity of the GnRH pulse generator. Again, the i.v. injection of glucose, but not saline, immediately recovered the decrease in the electrical activity of the GnRH pulse generator. Fructose could recover the activity of the GnRH pulse generator, but it took a longer time than glucose did. We suggest that glucose availability, but not simply a metabolic state such as the ATP level, is an essential factor for maintaining the electrical activity of the GnRH pulse generator which is responsible for pulsatile LH secretion.


Asunto(s)
Estradiol/farmacología , Glucosa/farmacología , Hormona Liberadora de Gonadotropina/metabolismo , Hipoglucemia/metabolismo , Insulina , Ovariectomía , Animales , Glucemia/análisis , Electrofisiología , Femenino , Hipoglucemia/inducido químicamente , Inyecciones Intravenosas , Insulina/farmacología , Hormona Luteinizante/sangre , Eminencia Media/efectos de los fármacos , Eminencia Media/fisiología , Flujo Pulsátil/efectos de los fármacos , Ratas , Ratas Wistar , Tiempo de Reacción/efectos de los fármacos
15.
Fertil Steril ; 60(3): 423-7, 1993 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-8375520

RESUMEN

OBJECTIVE: To define the hypersensitive status of PRL secretion in normoprolactinemic infertile women and determine the incidence of occult hyperprolactinemia among them. DESIGN: The potential for PRL secretion was examined in 463 women. SETTING: Outpatient clinic in a university hospital. PATIENTS: Three hundred sixty-seven infertile women and 96 healthy volunteers. INTERVENTIONS: Patients were treated with bromocriptine, 2.5 or 5 mg/d for 3 months. MAIN OUTCOME MEASURES: Prolactin response to thyrotropin-releasing hormone (TRH), circadian rhythm, and serum PRL changes during the menstrual cycle. RESULTS: Approximately 15% of infertile women showed an exaggerated response to TRH, and 95% among them had clinical disorders such as galactorrhea, luteal insufficiency, and menstrual disturbances. Bromocriptine proved effective in 90% of these women. Transient hyperprolactinemia was observed at night in 80% of normal PRL responders who had galactorrhea. Bromocriptine was effective in 75% of these women. Transient hyperprolactinemia during the menstrual cycle was observed in 43% of normal PRL responders with luteal insufficiency, 85% of whom responded to treatment with bromocriptine. CONCLUSION: In infertile women, the TRH test helps in the selection of patients who may respond to bromocriptine. Among normal PRL responders, 60% of patients with galactorrhea and 47% of those with luteal insufficiency recovered after bromocriptine treatment. From these results, patients who exhibit clinical abnormalities such as galactorrhea and luteal insufficiency should undergo extensive PRL testing.


Asunto(s)
Hiperprolactinemia/complicaciones , Infertilidad Femenina/sangre , Adulto , Bromocriptina/uso terapéutico , Cuerpo Lúteo/fisiopatología , Femenino , Galactorrea/sangre , Galactorrea/complicaciones , Humanos , Hiperprolactinemia/epidemiología , Incidencia , Infertilidad Femenina/tratamiento farmacológico , Infertilidad Femenina/fisiopatología , Fase Luteínica , Prolactina/sangre , Valores de Referencia , Hormona Liberadora de Tirotropina/farmacología
16.
Fertil Steril ; 62(2): 246-50, 1994 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-8034067

RESUMEN

OBJECTIVE: To examine changes in bone mineral density (BMD) of patients treated with GnRH agonist (GnRH-a) by dual-energy X-ray absorptiometry and to understand factors related to bone loss. DESIGN: Prospective controlled trial examining BMD during and after GnRH-a therapy every 24 weeks for 18 months in patients with endometriosis compared with nontreated controls. SETTING: Outpatients clinic at a university hospital and its affiliated outpatient clinic. PATIENTS: Twenty-two patients with endometriosis as GnRH-a-treated group, 12 healthy women with normal menstrual cycle, and 7 patients with mild endometriosis as control group. INTERVENTIONS: Patients were treated with a GnRH-a (buserelin acetate) at 900 micrograms/d by nasal spray for 24 weeks. RESULTS: The significance of differences in change-rates at all measured points in both groups was assessed by analysis of variance. The interaction between treatment and period was significant, and only week 24 of the GnRH-a-treated group was significantly lower compared with baseline. The reduction rate of BMD was high in patients 33 years of age or younger compared with those who were 34 years of age or older. According to a multiple-regression model, the most important factor related to bone loss was the post-treatment serum levels of E2. CONCLUSION: At the end of treatment, BMD was significantly lower than that of the control group, and the reduction rate was 3.4%. A factor related to bone loss was degree of ovarian suppression.


Asunto(s)
Densidad Ósea/efectos de los fármacos , Buserelina/uso terapéutico , Endometriosis/tratamiento farmacológico , Endometriosis/metabolismo , Adulto , Huesos/metabolismo , Femenino , Hormonas Esteroides Gonadales/sangre , Humanos , Estudios Prospectivos , Análisis de Regresión
17.
Fertil Steril ; 70(2): 246-52, 1998 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-9696215

RESUMEN

OBJECTIVE: To evaluate hyperprolactinemia in the pathogenesis of recurrent spontaneous abortion. DESIGN: Randomized trial. SETTING: Miscarriage clinic, Yokohama City University Hospital, Yokohama, Japan. PATIENT(S): From a group of 352 women with recurrent spontaneous abortion, we identified 64 patients with a prolactin disorder that was not associated with any other etiologic abnormalities, including ovarian or endocrinologic disturbances such as luteal phase dysfunction, polycystic ovaries, hypersecretion of LH, galactorrhea, or thyroid hormone disorders. INTERVENTION(S): Restoration of prolactin levels with bromocriptine. MAIN OUTCOME MEASURE(S): Successful pregnancy (live birth). RESULT(S): The percentage of successful pregnancies was higher in the bromocriptine-treated group than in the group that was not treated with bromocriptine (85.7% versus 52.4%, P < .05). Serum prolactin levels during early pregnancy (5-10 weeks of gestation) were significantly higher in patients who miscarried (31.8-55.3 ng/mL) than in patients whose pregnancies were successful (4.6-15.5 ng/mL, P < .01 or P < .05). CONCLUSION(S): Appropriate circulating levels of prolactin may play an important role in maintaining early pregnancy, especially in cases of hyperprolactinemic recurrent miscarriage.


Asunto(s)
Aborto Habitual/prevención & control , Bromocriptina/uso terapéutico , Antagonistas de Hormonas/uso terapéutico , Hiperprolactinemia/complicaciones , Resultado del Embarazo , Aborto Habitual/etiología , Adulto , Femenino , Humanos , Embarazo , Recurrencia
18.
Eur J Obstet Gynecol Reprod Biol ; 72(1): 89-92, 1997 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-9076428

RESUMEN

OBJECTIVE: To clarify a significance of prolactin (PRL) for infertility in endometriosis. STUDY DESIGNS: For seventy endometriotic patients with or without infertility, serum PRL concentrations measured by radioimmunoassay before and 30 min after 500 micrograms of thyrotropin-releasing hormone (TRH) injection were analyzed in relation to the Revised American Fertility Society score in endometriosis as well as to the outcome of the treatment for endometriotic infertility. RESULTS: While no significant relationship was found between the basal PRL levels and the stage of endometriosis or the outcome of the treatment for infertility, the PRL value after TRH injection was significantly greater in the patients who did not become pregnant than those who did (P < 0.05). CONCLUSIONS: Occult hyperprolactinemia may be involved in infertility in endometriotic patients.


Asunto(s)
Endometriosis/fisiopatología , Prolactina/metabolismo , Adolescente , Adulto , Endometriosis/complicaciones , Femenino , Humanos , Infertilidad Femenina/etiología , Infertilidad Femenina/terapia , Embarazo , Hormona Liberadora de Tirotropina , Resultado del Tratamiento
19.
Eur J Obstet Gynecol Reprod Biol ; 58(2): 207-9, 1995 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-7774752

RESUMEN

We describe a female infant with tracheal agenesis associated with severe complicated malformations including the cardiovascular system. The patient was born of a mother with mosaic Turner's syndrome at 35 weeks of gestation after premature rupture of the membranes during treatment for polyhydramnios. The patient died 2 days after birth and the autopsy disclosed tracheal agenesis and associated multiple anomalies.


Asunto(s)
Anomalías Múltiples/patología , Mosaicismo , Tráquea/anomalías , Síndrome de Turner , Adulto , Resultado Fatal , Femenino , Humanos , Recién Nacido , Cariotipificación , Mosaicismo/genética , Embarazo , Síndrome de Turner/genética
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA