RESUMEN
Light and electron microscopy studies of lactic dehydrogenase activity were carried out in embryonic, neonatal, and adult mouse lungs and in lungs undergoing chemically induced carcinogenesis. Embryonic mouse lungs were collected on the 6th, 12th, and 18th days of gestation; 1-day-old lungs were used for the neonatal model. These were compared with adult normal mouse lung and lungs of the animals treated with 4-nitroquinoline 1-oxide at a monthly interval until cancer developed. Enzymatic activity was seen in the embryonic, precancerous, and malignant lung tissues and was found diffusely in the cytoplasm of the epithelial cells.
Asunto(s)
L-Lactato Deshidrogenasa/metabolismo , Neoplasias Pulmonares/enzimología , Lesiones Precancerosas/enzimología , 4-Nitroquinolina-1-Óxido , Animales , Animales Recién Nacidos , Embrión de Mamíferos/enzimología , Femenino , Histocitoquímica , Pulmón/enzimología , Neoplasias Pulmonares/inducido químicamente , Ratones , Ratones Endogámicos A , Neoplasias Experimentales/enzimología , Lesiones Precancerosas/inducido químicamente , EmbarazoRESUMEN
Capsaicin (Sigma Chemical Co.) is a unique chemical agent that causes degeneration of afferent nerve fibers. Previous conclusions about Capsaicin effects on the gastric mucosal response to stress have not precisely defined which afferent nerves were affected. Therefore, the aim of the first portion of this study was to define the origin of afferent vagus nerves to the anterior gastric wall after injections of fluorogold (which is an axonal tracer) into the stomach. The second part of this study was to compare the stress effects on the gastric mucosa in rats with impaired afferent nerve function after Capsaicin treatment.