Consistency of magnetoencephalographic functional connectivity and network reconstruction using a template versus native MRI for co-registration.

INTRODUCTION: Studies using functional connectivity and network analyses based on magnetoencephalography (MEG) with source localization are rapidly emerging in neuroscientific literature. However, these analyses currently depend on the availability of costly and sometimes burdensome individual MR scans for co-registration. We evaluated the consistency of these measures when using a template MRI, instead of native MRI, for the analysis of functional connectivity and network topology. METHODS: Seventeen healthy participants underwent resting-state eyes-closed MEG and anatomical MRI. These data were projected into source space using an atlas-based peak voxel and a centroid beamforming approach either using (1) participants' native MRIs or (2) the Montreal Neurological Institute's template. For both methods, time series were reconstructed from 78 cortical atlas regions. Relative power was determined in six classical frequency bands per region and globally averaged. Functional connectivity (phase lag index) between each pair of regions was calculated. The adjacency matrices were then used to reconstruct functional networks, of which regional and global metrics were determined. Intraclass correlation coefficients were calculated and Bland-Altman plots were made to quantify the consistency and potential bias of the use of template versus native MRI. RESULTS: Co-registration with the template yielded largely consistent relative power, connectivity, and network estimates compared to native MRI. DISCUSSION: These findings indicate that there is no (systematic) bias or inconsistency between template and native MRI co-registration of MEG. They open up possibilities for retrospective and prospective analyses to MEG datasets in the general population that have no native MRIs available. Hum Brain Mapp, 2017. © 2017 The Authors Human Brain Mapping Published by Wiley Periodicals, Inc. Hum Brain Mapp 39:104-119, 2018. © 2017 Wiley Periodicals, Inc.

Brain Network Integration in Patients with Migraine: A Magnetoencephalography Study.

Migraine is a common disorder with high social and medical impact. Patients with migraine have a much higher chance of experiencing headache attacks compared with the general population. Recent neuroimaging studies have confirmed that pathophysiology in the brain is not only limited to the moment of the attack but is also present in between attacks, the interictal phase. In this study, we hypothesized that the topology of functional brain networks is also different in the interictal state, compared with people who are not affected by migraine. We also expected that the level of network disturbances scales with the number of years people have suffered from migraine. Functional connectivity between 78 cortical brain regions was estimated for source-level magnetoencephalography data by calculating the phase lag index, in five frequency bands (delta-beta), and compared between healthy controls (n = 24) and patients who had been suffering from migraine for longer than 6 years (n = 12) or shorter than 6 years (n = 12). Moreover, the topology of the functional networks was characterized using the minimum spanning tree. The migraine groups did not differ from each other in functional connectivity. However, the network topology was different compared with healthy controls. The results were frequency specific, and higher average nodal betweenness centrality was specifically evident in higher frequency bands in patients with longer disease duration, while an opposite trend was present for lower frequencies. This study shows that patients with migraine have a different network topology in the resting state compared with healthy controls, whereby specific brain areas have altered topological roles in a frequency-specific manner. Some alterations appear specifically in patients with long-term migraine, which might show the long-term effects of the disease.

The Hierarchy of Brain Networks Is Related to Insulin Growth Factor-1 in a Large, Middle-Aged, Healthy Cohort: An Exploratory Magnetoencephalography Study.

Recently, a large study demonstrated that lower serum levels of insulin growth factor-1 (IGF-1) relate to brain atrophy and to a greater risk for developing Alzheimer's disease in a healthy elderly population. We set out to test if functional brain networks relate to IGF-1 levels in the middle aged. Hence, we studied the association between IGF-1 and magnetoencephalography-based functional network characteristics in a middle-aged population. The functional connections between brain areas were estimated for six frequency bands (delta, theta, alpha1, alpha2, beta, gamma) using the phase lag index. Subsequently, the topology of the frequency-specific functional networks was characterized using the minimum spanning tree. Our results showed that lower levels of serum IGF-1 relate to a globally less integrated functional network in the beta and theta band. The associations remained significant when correcting for gender and systemic effects of IGF-1 that might indirectly affect the brain. The value of this exploratory study is the demonstration that lower levels of IGF-1 are associated with brain network topology in the middle aged.

The Relationship Between Job Satisfaction and Productivity-Related Costs: A Longitudinal Analysis.

OBJECTIVES: The aim of this study was to examine the longitudinal relationship between job satisfaction and total productivity-related costs, and between job satisfaction and absenteeism and presenteeism costs separately. A secondary aim was to explore whether these relationships differed across job types. METHODS: Linear generalized estimating equation analyses were used to explore the longitudinal relationships. To explore whether the relationships differed across job types, stratified analyses were performed. RESULTS: A significant relationship was found between job satisfaction and total productivity-related costs [ß = &OV0556;-273; 95% confidence interval (95% CI): -407 to -200] and between job satisfaction and presenteeism costs (ß = &OV0556;-276; 95% CI: -367 to -235), but not between job satisfaction and absenteeism costs. These relationships differed across job types. CONCLUSIONS: Higher levels of job satisfaction were longitudinally related to lower total productivity-related costs and presenteeism costs, but not to lower absenteeism costs. These relationships seem to differ across job types.

Relation between carotid stiffness, cognitive performance and brain connectivity in a healthy middle-aged population: an observational neurophysiological cohort study with magnetoencephalography.

OBJECTIVE: Impaired blood flow of the carotid artery can result in cognitive impairment, but how these vascular impairments lead to global cognitive disturbances is largely unknown. Problems in functional connectivity between brain areas may be responsible for these widespread effects. Therefore, the aim of this study was to examine the association between carotid stiffness, functional connectivity and cognitive performance in relatively young and healthy adults before clinical vascular pathology occurs. DESIGN: The Amsterdam Growth and Health Longitudinal Study: an observational study. SETTING: Participants were included by attending 1 of the 2 selected secondary schools in The Netherlands. PARTICIPANTS: Men (n=110) and women (n=120) aged 41-44 years (42±0.7). PRIMARY AND SECONDARY OUTCOME MEASURES: Data were obtained with regard to local carotid stiffness captured measured with the Young's elastic modulus (YEM). All participants underwent a commonly used Dutch intelligence test and resting-state eyes-closed magnetoencephalography (MEG). Five artefact-free epochs were analysed. The phase lag index (PLI) was used as a measure of functional connectivity between all sensors and was assessed in six frequency bands (δ-γ). RESULTS: Carotid stiffness was significantly associated with increased functional connectivity in the α2 band in men (ß: 0.287; p=0.008). The same results were found for women in the ß band (ß: 0.216; p=0.040). Furthermore, carotid stiffness was associated with superior cognitive function in men (ß: 0.238; p=0.007). In addition, there was neither a significant association nor a consistent pattern between cognitive function and functional connectivity. CONCLUSIONS: The increased connectivity might be a maladaptive phenomenon caused by disinhibition of neurons which may explain the direction of the results. This study suggests that detection of increased (local) carotid stiffness may be promising to identify a disturbance in the organisation of the functional brain network, even before clinical vascular pathology occurs.

A healthy brain in a healthy body: brain network correlates of physical and mental fitness.

A healthy lifestyle is an important focus in today's society. The physical benefits of regular exercise are abundantly clear, but physical fitness is also associated with better cognitive performance. How these two factors together relate to characteristics of the brain is still incompletely understood. By applying mathematical concepts from 'network theory', insights in the organization and dynamics of brain functioning can be obtained. We test the hypothesis that neural network organization mediates the association between cardio respiratory fitness (i.e. VO2 max) and cognitive functioning. A healthy cohort was studied (n = 219, 113 women, age range 41-44 years). Subjects underwent resting-state eyes-closed magneto-encephalography (MEG). Five artifact-free epochs were analyzed and averaged in six frequency bands (delta-gamma). The phase lag index (PLI) was used as a measure of functional connectivity between all sensors. Modularity analysis was performed, and both within and between-module connectivity of each sensor was calculated. Subjects underwent a maximum oxygen uptake (VO2 max) measurement as an indicator of cardio respiratory fitness. All subjects were tested with a commonly used Dutch intelligence test. Intelligence quotient (IQ) was related to VO2 max. In addition, VO2 max was negatively associated with upper alpha and beta band modularity. Particularly increased intermodular connectivity in the beta band was associated with higher VO2 max and IQ, further indicating a benefit of more global network integration as opposed to local connections. Within-module connectivity showed a spatially varied pattern of correlation, while average connectivity did not show significant results. Mediation analysis was not significant. The occurrence of less modularity in the resting-state is associated with better cardio respiratory fitness, while having increased intermodular connectivity, as opposed to within-module connections, is related to better physical and mental fitness.