RESUMEN
PURPOSE: A bacteriophage is a virus that infects and replicates within a bacterium following the injection of phage genome into the bacterial cytoplasm. They are seen as a possible therapy for multi-drug-resistant strains of many bacteria. The aim of this study is to evaluate the lytic activity of the Pyo, Intesti and Fersisi bacteriophage cocktails on P. aeruginosa and S. aureus. METHODS: Ten different S. aureus and P. aeruginosa strains, which were isolated from hospitalized patients in Turkey, were used in the study. The identification and antibiotic susceptibility of the isolates were performed using Vitec 2 system. The identities of the isolates were confirmed by a species-specific Polymerase Chain Reaction (PCR) assay. Lytic activity of the bacteriophage cocktails on bacteria was determined by spot test and plaque assay methods. RESULTS: The lytic activity of the Pyo phage cocktail was evaluated on P. aeruginosa and S. aureus strains. It was found that eight isolates of MDR S. aureus were susceptible to Pyo phage cocktail and two isolates were resistant. Nine isolates of antibiotic-resistant P. aeruginosa were found to be susceptible to this phage cocktail and one isolate was resistant. Thus, the Pyo, Intesti and Fersisi cocktails are very effective in treating clinical strains of multidrug-resistant P. aeruginosa and S. aureus isolated in Turkey. CONCLUSION: The Pyo, Intesti and Fersisi cocktails may prove useful in the treatment of various infections caused by those bacteria.
Asunto(s)
Bacteriólisis , Bacteriófagos/patogenicidad , Farmacorresistencia Bacteriana , Resistencia a Múltiples Medicamentos , Pseudomonas aeruginosa/virología , Staphylococcus aureus/virología , Antibacterianos/química , Infecciones Bacterianas/microbiología , Citoplasma/virología , Genoma Viral , Humanos , Tipificación Molecular , Reacción en Cadena de la Polimerasa , Análisis de Secuencia de ADN , Especificidad de la Especie , TurquíaRESUMEN
Retrospective research (2007-2012) was conducted on 500 patients with drug-resistant primary tuberculosis treated by DOTS-plus PS SLE Azerbaijan. The aim of the study was to establish a new strategy of identifying TB within the penitentiary system, aimed at early determination of the disease and soonest commencement of treatment of the patients with second generation medications. Results of the study as well as the analysis of multivariable indicators have confirmed that passive identification (ORa=1.13) of the patients and delayed commencement of the treatment by more than 3 months from determination (ORa=1.01), are the main prognosis factors of amplified drug resistance.