RESUMEN
Mutations in the human gene Jagged1 (JAG1) localized in 20p12 have been recently identified as causal for the anomalies found in patients with Alagille syndrome (AGS). This gene encodes a ligand for the Notch1 transmembrane receptor, which plays a key role in cell-to-cell signaling during differentiation and is conserved from C. elegans to human. We report a paracentric inversion (PAI) of chromosome 20p12.2p13 in an individual with AGS who also had alpha-1-antitrypsin deficiency. To our knowledge, this is the first published case of PAI involving the short arm of chromosome 20. Using FISH, fiberFISH, and molecular studies with a approximately 40 kb cosmid clone encompassing the entire 36 kb JAG1 gene, we demonstrate that the gene was disrupted by the inversion breakpoint between exons 5 and 6. An unusual association between two most common causes of chronic liver disease in childhood, AGS and alpha-1-antitrypsin deficiency, as well as their influence on the proband's abnormal phenotype are discussed.
Asunto(s)
Síndrome de Alagille/genética , Inversión Cromosómica , Cromosomas Humanos Par 20/genética , Proteínas/genética , Síndrome de Alagille/patología , Southern Blotting , Proteínas de Unión al Calcio , Preescolar , Bandeo Cromosómico , ADN/genética , Humanos , Hibridación Fluorescente in Situ , Lactante , Péptidos y Proteínas de Señalización Intercelular , Proteína Jagged-1 , Masculino , Proteínas de la Membrana , Mutación , Proteínas Serrate-JaggedRESUMEN
The frequency of occurrence of alpha-1-antitrypsin (A1AT) deficiency among total of 3228 Polish children with chronic liver diseases and chronic disease of respiratory tract was determined. It was observed that among children with chronic liver diseases which disclosed more frequent defect (concentration of A1AT below 150 mg/dl was found in 10.3% of children), the highest occurrence of deficiency was in children with neonatal hepatitis (23.1%). The deficiency was connected with the presence of ZZ and MZ phenotypes of A1AT.
Asunto(s)
Hepatopatías/sangre , Enfermedades Respiratorias/sangre , Deficiencia de alfa 1-Antitripsina , Adolescente , Niño , Preescolar , Hepatitis/sangre , Hepatitis/genética , Humanos , Lactante , Recién Nacido , Hepatopatías/genética , Fenotipo , Enfermedades Respiratorias/genética , alfa 1-Antitripsina/genéticaRESUMEN
OBJECTIVES: Delayed menarche, amenorrhea, early menopause have been sporadically reported in patients with CD. But control group matched was drawn from healthy individuals. DESIGN: The aim of this study was to investigate the age at menarche in patients with CD and in mothers of patients. MATERIALS: 59 mother/daughter pairs; daughter with coeliac disease: 49--treated with a gluten-free diet [FGD (+)] and 10 with untreated or late onset coeliac disease [FGD (-)]. METHODS: CD was diagnosed on the basis of ESPGAN criteria. All patients and mothers were asked for information on the age at menarche. RESULTS: The mean age of menarche was significantly higher in FGD (-): girls--mean 16.16 years and mother's--mean 15.49 years, than in FGD (+) patients. The mean age of menarche in FGD (+) patients and mother's were: 12.33 and 13.82 years respectively in the country (11 pairs), 13.08 and 13.49 years respectively in the little town (19 pairs), 12.90 and 13.33 years respectively in the town (19 pairs). CONCLUSIONS: The age at menarche in patients with CD and FGD is decreased to age at menarche in mother's, but is higher in the untreated CD patients and mother's. These findings support the hypothesis that the age at menarche in girls with coeliac disease is regulated by gluten-free diet and by other genetic and environmental factors.
Asunto(s)
Enfermedad Celíaca/diagnóstico , Menarquia/fisiología , Adolescente , Adulto , Factores de Edad , Niño , Femenino , Humanos , Bienestar Materno , Estudios Retrospectivos , Población Rural , Población UrbanaRESUMEN
OBJECTIVES: Abnormalities of liver function tests were been reported in adults with TS and in patients with oestrogen replacement therapy. DESIGN: The aim of this study was to evaluate the prevalence of liver disease in patients before or with oestrogen therapy. MATERIALS: 79 patients, aged 13(2/12) to 60(4/12), for oestrogen replacement therapy: 13--with secondary hypogonadism (group I), 29--with TS (group II), 19--with menopause (group IV) and 18--with TS, aged 2 to 16(11/12), before oestrogen therapy (group III). METHODS: All patients were tested for liver function tests (ALAT, GGTP, total bilirubin), and patients I-III groups for serum markers of HBV and HCV infection. RESULTS: The frequency of HBV/HCV infection was 38.3% in group I, 13.7%--in group II, 16.7%--in group III. In 13.7% patients of group II and in 16.7% patients of group III the liver disease had been classified as cryptogenic. CONCLUSIONS: The prevalence of HBV/HCV infection in patients with hypogonadism and the prevalence of cryptogenic liver disease in patients with TS, seems to be relatively high. In TS liver function test should be investigated as well before oestrogen therapy and should be turned during the follow up.
Asunto(s)
Terapia de Reemplazo de Estrógeno/métodos , Hepatitis B/epidemiología , Hepatitis C/epidemiología , Hipogonadismo/sangre , Hipogonadismo/terapia , Progestinas/uso terapéutico , Síndrome de Turner/sangre , Adulto , Alanina Transaminasa/sangre , Bilirrubina/sangre , Femenino , Estudios de Seguimiento , Hepatitis B/complicaciones , Hepatitis B/diagnóstico , Hepatitis C/complicaciones , Hepatitis C/diagnóstico , Humanos , Hipogonadismo/etiología , Pruebas de Función Hepática , Menopausia/fisiología , Prevalencia , Síndrome de Turner/complicaciones , gamma-Glutamiltransferasa/sangreRESUMEN
History data and clinical pattern were analysed in 33 children with herpetiform dermatitis (DH) and in 34 children with atopic dermatitis (DA). The differences are stressed between DH and DA as which it is frequently misdiagnosed, which is the cause of delayed treatment in DH (in the studied group 2.8 years on the average). The development of skin changes with accompanying itching in a child at preschool age should suggest the supposition of DH.
Asunto(s)
Dermatitis Herpetiforme/diagnóstico , Dermatitis Atópica/diagnóstico , Adolescente , Niño , Preescolar , Diagnóstico Diferencial , Femenino , Humanos , Lactante , MasculinoRESUMEN
A boy aged 1.5 year with deficient weight and height, retardation of motor development, decreased muscle tonus, finger tremor and periodic tachypnoea without detectable respiratory system changes is presented. Gasometry demonstrated metabolic acidosis with respiratory alkalosis, high concentration of lactic acid in serum and cerebrospinal fluid, increasing metabolic acidosis after glucose load, and lack of hyperglycaemic response after alanine load, and cortical atrophy in CT. On the basis of these changes Leigh's disease was diagnosed.
Asunto(s)
Enfermedad de Leigh/diagnóstico , Alanina , Glucemia/análisis , Humanos , Lactante , Lactatos/sangre , Ácido Láctico , Enfermedad de Leigh/sangre , MasculinoRESUMEN
On the basis of cooperation with the Children's Health Centre the role was analysed of certain phenotypes of alpha 1-antitrypsin (alpha 1-AT), mainly Pi Z and Pi MZ phenotypes in the development of infantile cirrhosis. A significant participation was demonstrated of the latter phenotype in patients developing infantile cirrhosis. The preliminary analysis of the incidence of pathological alpha 1-AT phenotypes in the group of patients with cirrhosis manifested at the adult age seems to indicate the higher incidence of the Pi MZ phenotype as compared with the general population. The genetically determined low serum alpha 1-AT activity is probably one out of many factors determining the development of this disease. Further studies will be aimed at explaining whether the combination of action of two factors: alpha 1-AT deficiency and exposure to hepatotoxic agents is not accelerating the appearance of the disease and is not potentiating its clinical intensity.
Asunto(s)
Cirrosis Hepática/etiología , Deficiencia de alfa 1-Antitripsina , Adolescente , Factores de Edad , Niño , Preescolar , Humanos , Lactante , Cirrosis Hepática/enzimología , Cirrosis Hepática/genética , Fenotipo , Factores de Tiempo , alfa 1-Antitripsina/genéticaRESUMEN
UNLABELLED: Magnesium deficiency has been reported in patients with classical coeliac disease. Classical coeliac disease has been recently very rare, but the frequency of the silent or latent form has increased. The aim of the study was to evaluate the magnesium status in patients with coeliac disease diagnosed according to ESPGAN criteria. 41 GFD(+) patients aged 6-18 years, who were on a gluten-free diet (GFD) for 2.8 to 17.3 years (mean 11 years); with normal villous structure and IgAEmA(-), and 32 GFD(-) patients aged 5-17 years, with villous atrophy and IgAEmA(+): 8--after 7/12-13/12 of gluten challenge, 4--with late onset of coeliac disease, 20--with silent coeliac disease. All of the children did not have any other disorders. Magnesium status was examined by using: an i.v. Mg-loading test (30 mmol/1.73 m2); Mg urinary excretion and Mg concentration in serum, erythrocytes, and in hair. Abnormal values in GFD(+) and GFD(-) patients were found in: Mg i.v. loading test (retention > 40%) in 20 vs 34%, serum Mg (< 0.7 mmol/l) in 7 vs 3%, erythrocytes Mg (< 1.8 mmol/l) in 20 vs 25%. The reversed statistically significant correlation was found between Mg retention and Mg urinary excretion (R = -0.293, p = 0.009). No other statistically significant correlations were found. CONCLUSION: The magnesium deficiency was present in all patients with classical coeliac disease, but only in 1/5 of patients with coeliac disease on a gluten-free diet and in 1/5 of patients with silent coeliac disease.
Asunto(s)
Enfermedad Celíaca/complicaciones , Deficiencia de Magnesio/complicaciones , Magnesio/metabolismo , Adolescente , Enfermedad Celíaca/metabolismo , Niño , HumanosRESUMEN
UNLABELLED: Magnesium (Mg) deficiency is often noted in patients with coeliac disease (CD). The aim of the study was the analysis of the reasons of this deficiency in children with CD, diagnosed according to ESPGAN criteria. MATERIAL: The study was performed on 41 patients aged 6-18 years adhering to strict gluten-free diet GFD(+) for mean 11 years, with normal small intestine mucosa, and IgAEmA(-), and on 32 patients aged 5-17 years on gluten containing diet, with classical CD, silent CD or after gluten challenge--GFD(-). In this group the villous atrophy of the small intestine and IgAEmA(+) were observed. In 18 of these patients Mg deficiency was found using Mg-loading test (30 mmol/1.73 m2). METHODS: The following parameters were analysed: type of the disease, observance of gluten-free diet, sex, and living place. Mg, Ca, Na, protein, fat, and dietary fiber intake was assessed using food frequency questionnaire method, and steatorrhea using faecal fat excretion (g/24 h). RESULTS: The frequency of Mg deficiency was similar in both sexes, occasionally in children from small towns (4.5%), and more often in children from big cities (31.5%), and village (34.4%). Dietary Mg intake below RDA was observed in 23% of children from GFD(+) group, in 19% from GFD(-) one, and in 17.6% in children with Mg deficiency. Insufficient Mg intake was found in 18.2% of children from small towns, in 17.6% from big cities, and in 12.5% from villages; Ca in 36.6%, 58.8%, and 59.3%, and protein in 18.2%, 35.3%, and in 34.4% respectively. In all groups of children high intake of fat and Na was observed. Dietary fiber intake was within the recommended values. All children with classical CD had increased fat excretion (mean 25.9 g/24 h), in other patients it was within normal values [GFD(+) mean 1.95 g/24 h, in GFD(-) without diarrhoea 1.7 g/24 h. CONCLUSIONS: Magnesium deficiency in children with CD depends on the form of the disease, adhering to GFD, diarrhoea with steatorrhea, and/or low Mg intake with the diet.