Chemical analysis of n-propyl gallate used as pre-treatment for resin-dentin bond strength: In vitro study.

This study aimed to evaluate the effect of n-propyl gallate as pre-treatment for resin-dentin bond strength. The dentin pre-treatments evaluated included propyl gallate of concentrations 0.1% (w/v), 1.0% (w/v), and 10.0% (w/v), as well as glutaraldehyde 5.0% (v/v), and distilled water as a control treatment. Dentin specimens were prepared for Fourier Transformed Infrared Spectroscopy (FT-IR) (n = 3/pre-treatment). Pre-treatments were actively applied to dentin blocks before performing the adhesive procedure to composite resin. Microtensile bond strength to dentin (µTBS) (n = 8/pre-treatment) was determined after 24 h and 6 months of storage. Data were submitted to a two-way ANOVA, followed by Tukey's post hoc test. As for FT-IR, propyl gallate 1%-treated specimens presented higher water, carbonate, collagen, and amide absorbance rates compared to other tested groups, while specimens pre-treated with glutaraldehyde and distilled water presented similar absorbance curves. Regarding µTBS, all concentrations of propyl gallate resulted in statistically significant higher bond strength values than distilled water at 24 h. After 6 months of storage, propyl gallate 0.1% was the only group that maintained µTBS over time. Propyl gallate 0.1% might be a suitable dentinal pre-treatment due to being able to present chemical bonds with demineralized dentin and providing resin-dentin bond stability after 6 months of storage.

Dentin bonding and physicochemical properties of adhesives incorporated with epigallocatechin-3-gallate.

The aim was to assess dentin adhesion and physicochemical properties of experimental etch-and-rinse and self-etch adhesives doped with epigallocatechin-3-gallate (EGCG), a well-known collagen crosslinker obtained from green tea (Camellia sinensis). Experimental adhesives were prepared without (0 wt%), with 0.1 or 0.5 wt% EGCG addition. Their degree of conversion was surveyed by FTIR, and bar-shaped specimens were tested to obtain flexural strength and modulus initially and after 1-week ethanol storage. Extracted human molars were prepared, bonded, and cut into resin-dentin sticks for microtensile bond strength test, which was conducted after 24 h or 6-month water storage. Statistical analyses were performed with two-way ANOVA and Tukey's test (p < 0.05). Degree of conversion outcomes depicted a significant polymerization reduction by the addition of EGCG in self-etch adhesive in both concentrations. However, only 0.1% reduced the conversion of etch-and-rinse adhesive. Flexural modulus and strength were significantly diminished (p < 0.05) by the addition of both concentrations of EGCG for the two model adhesives. Dentin-bond strength was reduced after aging with the addition of EGCG to self-etch adhesive. Nevertheless, 0.5% EGCG increased the bond strength of etch-and-rinse adhesive after aging, conversely to the significant reduction for EGCG-free control adhesive. In conclusion, EGCG at 0.5% provides optimal improvements on dentin bonding without altering final polymerization of a model etch-and-rinse adhesive.

Pretreatment with Sodium Fluoride Maintains Dentin Bond Strength of a Two-Step Self-Etch Adhesive after Thermal Stressing.

PURPOSE: To evaluate the effect of sodium fluoride and chlorhexidine digluconate on the composite-dentin bond strength of a self-etch adhesive after thermocycling. MATERIALS AND METHODS: Eighteen human third molars were prepared to expose a flat dentin surface and were divided into 3 groups (n = 6) according to the 3 cavity cleaning solutions: distilled water, 2% chlorhexidine digluconate (CHX), or 1.23% sodium fluoride (NaF). Solutions were rubbed onto dentin surfaces for 60 s, followed by bonding with Clearfil SE Bond, and a 5-mm-thick composite crown buildup. Bonded teeth were stored in distilled water for 24 h and then longitudinally sectioned to obtain bonded sticks. Half of the specimens were immediately tested in tension at 0.5 mm/min, while the remaining specimens were tested after 60,000 thermal cycles. Data were analyzed using two-way ANOVA and the Holm-Sidak method. RESULTS: There was no significant difference between the groups after 24 h (p > 0.05). Thermocycling resulted in significant bond strength reduction for distilled water and CHX (p < 0.05). When 24 h bond strengths were compared to the thermocycling group, NaF maintained its bond strength (p > 0.05), while significant reductions were observed for distilled water and CHX (p < 0.05). CONCLUSIONS: Pretreatment with NaF maintained the bond strength of Clearfil SE Bond to dentin after 60,000 thermal cycles, but pretreatment with CHX did not.

Randomized Two-year Clinical Evaluation of Oxalic Acid in Restorations of Noncarious Cervical Lesions.

PURPOSE: To evaluate the use of oxalic acid in restorations of noncarious cervical lesions. MATERIALS AND METHODS: Twenty volunteers of both sexes between 24 and 55 years old, with at least two lesions that were restored with the techniques to be evaluated, were selected. Ninety teeth were randomly divided into two groups: group 1, two-step etch-and-rinse adhesive technique (control) and group 2, pretreatment with oxalic acid followed by the application of a two-step etch-and-rinse adhesive system. The adhesive system used was XP Bond (Dentsply) and the restorative composite resin was Durafill (Heraeus Kulzer). A modified USPHS method was employed for the clinical evaluation, taking into account the following criteria: retention (R), marginal integrity (MI), marginal discoloration (MD), postoperative sensitivity (S), caries (C), and anatomic form (AF). Clinical assessments were conducted by two examiners at baseline and after 6 months, 1 and 2 years. RESULTS: After two years, the results of clinically satisfactory restorations (Alpha and Bravo) obtained for the control and experimental groups were: R (90.9%/93.33%), MI (100%/100%), MD (100%/100%), S (100%/100%), C (100%/100%), AF (100%/100%). CONCLUSION: Over a 2-year period, the use of oxalic acid as an agent of dentin pretreatment did not influence the clinical performance of restorations in noncarious cervical lesions.

Four-year randomized clinical trial of oxalic acid pretreatment in restorations of non-carious cervical lesions.

OBJECTIVE: The aim of this study was to evaluate the efficiency of oxalic acid (BisBlock) on restorations of non-carious cervical lesions. MATERIALS AND METHODS: One operator placed 90 restorations randomly divided into two groups in 20 patients under cotton rolls isolation: Control Group-two-step etch-and-rinse adhesive technique; and Experimental Group-two-step etch-and-rinse adhesive technique with oxalic acid pretreatment after acid-etched dentin. The restorative adhesive system used was XP Bond/Durafill. The restorations were directly assessed by two independent examiners, using the modified United States Public Health Service (USPHS) criteria at the baseline and 4 years. Data were statistically analyzed using the Fisher and McNemar tests (p < 0.05). RESULTS: Fifteen patients (75%) were available for recall after 4 years. The McNemar test detected significant differences within the Experimental Group between the baseline and 4-year evaluations for retention (p < 0.05). For the Control Group, there was no significant difference (p > 0.05) between the periods. The Fisher test showed no statistically significant difference between the groups for all other criteria (p > 0.05). CONCLUSION: After 4 years of service, the use of oxalic acid did not influence the clinical performance of restorations when it was used under composite resin restorations.

Effect of Whitening Toothpastes on Dentin Abrasion: An In Vitro Study.

PURPOSE: To compare the effect of toothbrushing abrasion with hydrated silica-based whitening and regular toothpastes on root dentin using contact profilometry. MATERIALS AND METHODS: Ninety dentin specimens (4 x 4 x 2 mm) were randomly divided into five experimental groups (n = 18) according to the toothpaste: three whitening (W1, W2 and W3) and two regular toothpastes (R1 and R2) produced by two different manufacturers. Using a brushing machine, each specimen was brushed with a constant load of 300 g for 2500 cycles (4.5 cycles/s). The toothpastes were diluted at a ratio of 1:3 w/w (dentifrice:distilled water). The brush diamond tip of the profilometer moved at a constant speed of 0.05 mm/s with a force of 0.7 mN. RESULTS: The average value of brushing abrasion in µm (mean ± SD) was obtained from five consecutive measurements of each specimen: W1 = 8.86 ± 1.58, W2 = 7.59 ± 1.04, W3 = 8.27 ± 2.39, R1 = 2.89 ± 1.05 and R2= 2.94 ± 1.29. There was a significant difference between groups (ANOVA, p<0.0001). Post-hoc Tukey's test for multiple comparisons showed differences between all the whitening and regular toothpastes, but not among the whitening nor among the regular toothpastes. CONCLUSION: The whitening toothpastes tested can cause more dentin abrasion than the regular ones.

Effect of commercial fluoride dentifrices against hydrochloric acid in an erosion-abrasion model.

OBJECTIVES: This study assessed the effect of three commercial dentifrices with different fluoride-containing compounds in controlling the progression of dentin loss using an in vitro erosion-abrasion model. MATERIALS AND METHODS: Dentin specimens were randomized into four groups (n = 10): control (no F), Elmex (1,400 ppm AmF), Meridol (1,400 ppm AmF/SnF2), and Crest Pro-Health (1,100 ppm SnF2). The dentin specimens were submitted to cycles of demineralization (HCl 0.01 M for 60 s), remineralization (artificial saliva for 60 min), and immersion in 1:3 w/w of dentifrice/artificial saliva, followed by toothbrushing (150 brushing strokes). The cycle was repeated three times daily for 5 days. Surface loss was quantified by stylus profilometry. Data were submitted to one-way ANOVA and Tukey's tests (p < 0.05). RESULTS: Wear (µm ± SD) was control 4.1 ± 1.2, Elmex 3.7 ± 1.5, Meridol 1.3 ± 0.4, and Crest Pro-Health 2.1 ± 0.7. Therefore, all products (except Elmex) produced statistically significantly less mineral loss (p < 0.05) when compared with the control. CONCLUSION: None of the dentifrices avoided the erosive-abrasive process; however, SnF2-containing dentifrices were effective in statistically significantly reducing dentin loss. CLINICAL RELEVANCE: Scientific literature shows evidence that fluoride can strengthen dental tissue against erosive acid damage. However, the beneficial effect of different fluorides present in commercial dentifrices is questionable. Thus, a determination of an effective fluoride dentifrice may be beneficial in the reduction of the erosive process in patients with gastric disorders.

Preemptive use of ibuprofen and desensitizer decreases immediate tooth sensitivity after in-office bleaching: A triple-blind, randomized, placebo-controlled clinical trial.

BACKGROUND: The authors evaluated the synergistic analgesic effect of preemptive administration of 400 mg of ibuprofen (IBU) and potassium fluoride 2% (KF2), both in isolation or combination, on the painful perception of tooth sensitivity after tooth bleaching. METHODS: Fifteen patients participated in this triple-blind, randomized, placebo-controlled clinical trial. The study used a crossover design for drug administration and a split-mouth design for desensitizer agent. Four paired groups were formed: IBU plus KF2 (IBU + KF2); IBU (IBU + placebo KF); KF2 (placebo IBU + KF2), and placebo (placebo IBU + placebo KF). The outcome measure was the perception of tooth sensitivity, assessed using a visual analog scale. Data were collected at 4 different times: immediately after tooth bleaching (baseline) and after 6, 30, and 54 hours. Statistical analysis was performed using the Friedman test and relative risk. RESULTS: IBU plus KF2 was found to be more effective in reducing tooth sensitivity immediately after bleaching (baseline) compared with the placebo group (P < .05). The risk of experiencing moderate or severe tooth sensitivity was approximately 4 times higher in the placebo group than in the IBU plus KF2 group (relative risk, 4.00; 95% CI, 1.01 to 15.81; P = .025). CONCLUSIONS: The combined use of 400 mg of IBU and KF2 appears to be beneficial in managing postbleaching tooth sensitivity. It provides a superior analgesic effect compared with placebo. PRACTICAL IMPLICATIONS: The preemptive administration of IBU plus KF2 reduces tooth sensitivity after tooth whitening when compared with placebo. This clinical trial was registered in the Brazilian Clinical Trials Registry Platform. The registration number is U1111-1249-8191.

Effect of the flavonoid epigallocatechin-3-gallate on resin-dentin bond strength.

PURPOSE: To evaluate the effect of dentin pretreatment with epigallocatechin-3-gallate solution on the preservation of the resin/dentin interface with etch-and-rinse adhesives. MATERIALS AND METHODS: Thirty extracted human molars were prepared to expose the dentin surface and divided into 5 groups according to the pretreatment solution. Dentin surfaces were etched (35% phosphoric acid for 15 s), rinsed, and air dried. Dentin was rewetted either with distilled water, 3 different epigallocatechin-3-gallate solutions (EGCG; 0.02%, 0.1%, or 0.5% w/v), or 2% chlorhexidine digluconate solution for 60 s. Adper Single Bond 2 was applied and a subsequent 5-mm-thick resin crown was built up. Bonded teeth were longitudinally sectioned to obtain sticks with a cross-sectional area of 1.0 mm2. Half of the specimens were immediately tested, while the remaining specimens were tested after storage in 3 mMol/l sodium azide solution at 37°C for six months. The mode of fracture was examined. Bond strength values were analyzed with ANOVA and Student-Newman-Keuls tests. RESULTS: After 24 h of storage, mean bond strengths of 0.5% EGCG were significantly lower than those found for the other groups (p < 0.05), except for 0.02% EGCG (p > 0.05). Resin-dentin bond strengths after 6 months were not significantly different among the experimental groups (p > 0.05). Storage in water for 6 months resulted in a significant decrease in bond strength for the water control group only (p < 0.05). The bond strengths of the experimental groups remained stable after 6 months of water storage (p > 0.05). CONCLUSION: Pretreatment with EGCG preserved the bond of Adper Single Bond 2 to dentin after six months of storage equally well as pretreatment with chlorhexidine digluconate.

Effects of 2 polishing techniques and reglazing on the surface roughness of dental porcelain.

The aim of this study was to compare the effect of 2 polishing systems and reglazing of dental porcelain through a quantitative and qualitative analysis of surface roughness using a stylus profilometer and scanning electron microscope. Fifteen porcelain specimens (10 x 3 x 3 mm) were used. On 1 surface of each block, a layer of glaze was applied, and surface roughness (Ra) was analyzed. All specimens were ground with aluminum oxide sandpaper until the shine was removed and the resulting Ra values were obtained. Afterwards, they were randomly divided into 3 treatment groups (n = 5): Group I (GI), polished with diamond-impregnated rubber wheels; Group II (GII), polished with silicon carbide-impregnated rubber wheels; and Group III (GIII), reglazed firing procedure alone. After the treatments, new Ra measurements were done. Data were submitted to analysis of variance (ANOVA), and Tukey tests at 5%. Comparisons between ground surface and treated surface were made by paired t-test. The ground and treated Ra values (µm) were determined as follows: GI: 0.66 ± 0.14, 0.35 ± 0.06; GII: 0.60 ± 0.04, 0.09 ± 0.03; and GIII: 0.67 ± 0.05, 0.75 ± 0.24. Significant differences were found between the ground and treated values for all groups. After the treatments, all groups differed statistically (P < 0.05). The silicon carbide system re-established the initial surface smoothness, while polishing with diamond-impregnated rubber or reglazing alone were not able to achieve a satisfactory smoothness.

A conservative treatment approach using direct composite resins for anterior teeth eroded by lemon sucking.

An excessively acidic diet results in the progressive deterioration of dental health, with functional, esthetic, and biological consequences. Previously, rehabilitation required placing numerous full crowns and root canal treatments; however, with improved adhesive techniques, a more conservative approach may be utilized to preserve tooth structure. This article describes 2 cases that utilized conservative dental treatments (involving direct composite resins with minimal preparation of the tooth structure) to treat eroded dentition induced by lemon sucking.

Restoring esthetics in eroded anterior teeth: a conservative multidisciplinary approach.

Consuming a highly acidic diet can lead to erosion or excessive wear of dental hard tissues, resulting in the need for oral rehabilitation. Previously, a severely eroded dentition could be rehabilitated only by extensive crown and bridge placement or by removable partial dentures. However, developments in adhesive dentistry have made it possible to restore the esthetics and function of eroded teeth in a minimally invasive manner. This case report presents a conservative, multidisciplinary approach to restoring severe wear due to dental erosion using proper techniques and materials.

Hesperidin reduces dentin wear after erosion and erosion/abrasion cycling in vitro.

OBJECTIVE: To evaluate the action of hesperidin (HPN) at different concentrations to prevent dentin erosive wear, associated or not to abrasion. METHODS: A study with 6 experimental groups (n = 10) for erosion (experiment 1) and another 6 for erosion + abrasion (experiment 2). The treatments were: distilled water (DW), DW with collagenase (DW + Col), 0.46% epigallocatechin-3-gallate (EGCG) and 0.1%, 0.5% or 1% HPN. The specimens were submitted to a cycle (3x/day) for 5 days that consisted of immersion on 1% citric acid (5 min), artificial saliva (60 min), treatment (5 min), brushing (150 movements only in experiment 2), and artificial saliva (60 min / overnight). Collagenase was added in artificial saliva for all groups except DW-group. Dentin changes were assessed with optical profilometry and scanning electron microscopy. Data were submitted to one-way analysis of variance and Tukey tests (α = 0.05). RESULTS: For experiment 1, DW showed the lowest wear and did not significantly differ from EGCG. DW + Col showed the highest wear, being significantly different from HPN at 1%. In experiment 2, DW showed the lowest wear and DW + Col the highest. EGCG showed less wear than the three groups treated with HPN. In addition, for both cycling models, there were no significant differences among the three concentrations of HPN analyzed. In micrographs of HPN-treated groups, it could be observed the formation of a barrier on the dentin that promoted the obliteration of the tubules. CONCLUSIONS: HPN was able to preserve the demineralized organic matrix layer but did not overcome the effect of EGCG.

Protective effect of green tea catechins on eroded human dentin: an in vitro/in situ study.

The present study sought to evaluate the protective effect of Epigallocatechin-3-gallate (EGCG) and commercial green tea (GT) on eroded dentin using in vitro and in situ experimental models. For the in vitro experiment, matrix metalloproteinases (MMPs) were extracted from demineralized human coronary dentin powder (citric acid, pH 2.3) and assessed via a colorimetric assay and electrophoresis in gelatin. The gels were exposed to buffers with: control (no treatment), 0.05% sodium fluoride (NaF), 0.12% chlorhexidine digluconate (CHX), GT infusion, and 0.1% EGCG, and their respective activity was analyzed by zymography. For the in situ experiment, 20 healthy volunteers (aged 20-32 years) participated in this single-center, blind, crossover study. The subjects wore upper removable devices containing four human dentin blocks. Erosive challenge (coke-1 min) was performed four times/day/5 days. Blocks were treated for 1 min with: control (No treatment), 0.05% NaF, 0.1% EGCG, and GT. Thereafter, the specimens were subjected to stylus profilometry and SEM. ANOVA was used to evaluate dentin roughness and wear, with a significance level of 5%. In the zymography analysis, 0.12% CHX, GT, and 0.1% EGCG were found to inhibit the action of MMPs; however, in the colorimetric assay, only green tea inhibited the activity of MMPs. There were no significant differences observed in dentin roughness or wear (p > 0.05). Herein, EGCG and GT inhibited the activity of endogenous proteases, resulting in protection against erosion-induced dentin damage; however, they could not prevent tooth tissue loss in situ.

Protective effect of NaF/triclosan/copolymer and MFP dentifrice on enamel erosion.

PURPOSE: To evaluate in situ the protective effect of sodium fluoride (NaF)/triclosan/copolymer dentifrice compared with monofluorphosphate (MFP) dentifrice on eroded enamel. METHODS: The specimens were subjected to erosion with cola soft drink for 60 seconds, four times a day, for 5 days, followed by treatment with the slurry of nonfluoridated, NaF (1450 ppm F/triclosan/copolymer) or MFP (1450 ppm F) dentifrices. Their effects were assessed by surface hardness loss. RESULTS: The dentifrice containing NaF/triclosan/copolymer was statistically more effective on reduction of enamel acid erosion than the non-fluoride and the MFP dentifrice (P < 0.05).

Direct resin composite restorations versus indirect composite inlays: one-year results.

AIM: The aim of this study was to evaluate the clinical performance of direct resin composite restorations (Tetric Ceram-TC) and indirect composite inlays (Targis-TG) after 12 months. METHODS AND MATERIALS: Seventy-six Class I and II restorations (44 direct and 32 indirect) were inserted in premolars and molars with carious lesions or deficient restorations in 30 healthy patients according to the manufacturer's instructions. Each restoration was evaluated at baseline and after 12 months according to the modified USPHS criteria for color match (CM), marginal discoloration (MD), secondary caries (SC), anatomic form (AF), surface texture (ST), marginal integrity (MI), and pulp sensitivity (PS). Data were analyzed by Fisher and McNemar Chi-square tests. RESULTS: No secondary caries and no pulpal sensitivity were observed after 12 months. However, significant changes in marginal discoloration (MD) criteria could be detected between baseline and one-year results for both materials (p<0.05). For marginal integrity (MI) criteria, the differences between baseline and one-year recall were statistically significant (p<0.05). For marginal integrity (MI) criteria, Tetric Ceram (TC) showed results statistically superior to Targis (TG) in both observation periods (p<0.05). No statistically significant changes in color match (CM), anatomic form (AF), or surface texture (ST) appeared during the observation periods (p>0.05). CONCLUSIONS: BDirect resin composite restorations performed better than indirect composite inlays for marginal integrity, but all restorations were judged to be clinically acceptable. CLINICAL SIGNIFICANCE: Tetric Ceram direct restorations and Targis indirect inlays in posterior teeth provide satisfactory clinical performance and the comparison between them showed little difference after one year.

Two-year clinical evaluation of a proanthocyanidins-based primer in non-carious cervical lesions: A double-blind randomized clinical trial.

OBJECTIVES: This double-blind randomized clinical trial evaluated the influence of pre-treatment with proanthocyanidins (PA) from grape seed extract on the clinical behavior of a simplified etch-and-rinse adhesive placed in non-carious cervical lesions (NCCLs) over 6- and 24-months. MATERIALS AND METHODS: A total of 135 restorations were randomly inserted in 45 subjects. The NCCLs were etched with 37 % phosphoric acid for 15 s and distributed into 3 groups: Control (PA0) - adhesive ExciTE F applied as per the manufacturer's recommendations; PA2 and PA5 groups - 2 wt% and 5 wt% PA solution, respectively, were applied for 60 s and washed for 30 s prior to application of the adhesive. The resin composite was placed incrementally and light-cured. The restorations were evaluated at baseline, 6 months (6 m) and 24 months (24 m) using both the FDI and USPHS criteria. Statistical analyses were carried out using Friedman repeated-measures analysis of variance by rank and the Wilcoxon test (α = 0.05). RESULTS: The retention rates were 98 % (PA0), 98 % (PA2) and 83 % (PA5) after 6 m and 93 % (PA0), 89 % (PA2) and 70 % (PA5) after 24 m. Only PA5 resulted in a significant lower retention rate at 6 m and at 24 m compared with that of baseline (p = 0.03). All groups resulted in a significantly worse marginal adaptation and marginal staining for the FDI criteria when the baseline vs. the 24 m recall data were compared. These differences were considered clinically acceptable under the FDI criteria. CONCLUSIONS: The application of PA as a primer did not result in clinical advantages after 24 m of clinical service, regardless of the concentration used. CLINICAL RELEVANCE: It has been reported that PA, a collagen crosslinking agent, increases the durability of the dentin-resin interface. However, no effects were found clinically after 24 months.

Management of dentinal hypersensitivity.

Dentinal hypersensitivity is a complex condition that can cause considerable concern in the dental office. Despite the large number of available treatment modalities, no current desensitizing agent is considered ideal for managing this uncomfortable condition. Selecting the right therapy requires a complete understanding of how applying a stimulus to the exposed dentin surface can influence the nerve fibers and produce hyperesthesia. This article reviews the etiology and critically analyzes management of dentinal hypersensitivity by reviewing laboratory and clinical investigations.

Effect of Metalloproteinase Inhibitors on Bond Strength of a Self-etching Adhesive on Erosively Demineralized Dentin.

PURPOSE: To analyze the influence of epigallocatechin-3-gallate (EGCG) and chlorhexidine (CHX) on adhesive-dentin bond strength of a self-etch adhesive to sound dentin (SD) and eroded dentin (ED). MATERIALS AND METHODS: Thirty-six middle-dentin samples were assigned to six groups (n = 6) according to pretreatment (DW: distilled water, control; 0.1% EGCG; or 2% CHX) and erosive challenge (presence or absence). Specimens were subjected to 2-h acquired pellicle formation, then half of them were exposed to 1% citric acid three times a day for five days. SD and ED were treated with the tested solutions for 60 s, and then Clearfil SE Bond was applied before resin composite buildup. Bonded teeth were longitudinally sectioned into sticks and half were immediately tested, while the remaining specimens were tested after six months. The mode of fracture was examined and the microtensile bond strength (µTBS) measured. Statistical analysis was performed with ANOVA and Bonferroni tests. RESULTS: At both time periods, regardless of the dentin substrate, EGCG groups did not show bond strengths that were significantly different from those obtained with DW (p > 0.05), while CHX generated lower values than did DW (p < 0.05). On SD, there was a bond strength reduction only in the CHX groups after six months. However, for ED, the bond strength significantly decreased in all groups. CONCLUSION: CHX negatively affected both dentin substrates, while the pretreatment with EGCG did not affect µTBS over time on SD. µTBS may be influenced by the substrate over time and EGCG can be used as an alternative to CHX to maintain the bond strength of self-etching adhesives.