RESUMEN
BACKGROUND: Chemerin, encoded by the retinoic acid receptor responder 2 (RARRES2) gene is an adipocytesecreted protein with autocrine/paracrine functions in adipose tissue, metabolism and inflammation with a recently described function in vascular tone regulation, liver, steatosis, etc. This molecule is believed to represent a critical endocrine signal linking obesity to diabetes. There are no data available regarding evolution of RARRES2 in non-human primates and great apes. Expression profile and orthology in RARRES2 genes are unknown aspects in the biology of this multigene family in primates. Thus; we attempt to describe expression profile and phylogenetic relationship as complementary knowledge in the function of this gene in primates. To do that, we performed A RT-PCR from different tissues obtained during necropsies. Also we tested the hypotheses of positive evolution, purifying selection, and neutrality. And finally a phylogenetic analysis was made between primates RARRES2 protein. RESULTS: RARRES2 transcripts were present in liver, lung, adipose tissue, ovary, pancreas, heart, hypothalamus and pituitary tissues. Expression in kidney and leukocytes were not detectable in either species. It was determined that the studied genes are orthologous. CONCLUSIONS: RARRES2 evolution fits the hypothesis of purifying selection. Expression profiles of the RARRES2 gene are similar in baboons and chimpanzees and are also phylogenetically related.
Asunto(s)
Evolución Molecular , Pan troglodytes/genética , Papio/genética , Receptores de Ácido Retinoico/genética , Animales , Secuencia de Bases , Femenino , Masculino , Datos de Secuencia Molecular , Filogenia , Reacción en Cadena de la Polimerasa de Transcriptasa InversaRESUMEN
Two Gram-negative, non-motile, non-spore-forming coccoid bacteria (strains F8/08-60(T) and F8/08-61) isolated from clinical specimens obtained from baboons (Papio spp.) that had delivered stillborn offspring were subjected to a polyphasic taxonomic study. On the basis of 16S rRNA gene sequence similarities, both strains, which possessed identical sequences, were assigned to the genus Brucella. This placement was confirmed by extended multilocus sequence analysis (MLSA), where both strains possessed identical sequences, and whole-genome sequencing of a representative isolate. All of the above analyses suggested that the two strains represent a novel lineage within the genus Brucella. The strains also possessed a unique profile when subjected to the phenotyping approach classically used to separate species of the genus Brucella, reacting only with Brucella A monospecific antiserum, being sensitive to the dyes thionin and fuchsin, being lysed by bacteriophage Wb, Bk2 and Fi phage at routine test dilution (RTD) but only partially sensitive to bacteriophage Tb, and with no requirement for CO2 and no production of H2S but strong urease activity. Biochemical profiling revealed a pattern of enzyme activity and metabolic capabilities distinct from existing species of the genus Brucella. Molecular analysis of the omp2 locus genes showed that both strains had a novel combination of two highly similar omp2b gene copies. The two strains shared a unique fingerprint profile of the multiple-copy Brucella-specific element IS711. Like MLSA, a multilocus variable number of tandem repeat analysis (MLVA) showed that the isolates clustered together very closely, but represent a distinct group within the genus Brucella. Isolates F8/08-60(T) and F8/08-61 could be distinguished clearly from all known species of the genus Brucella and their biovars by both phenotypic and molecular properties. Therefore, by applying the species concept for the genus Brucella suggested by the ICSP Subcommittee on the Taxonomy of Brucella, they represent a novel species within the genus Brucella, for which the name Brucella papionis sp. nov. is proposed, with the type strain F8/08-60(T) (â=âNCTC 13660(T)â=âCIRMBP 0958(T)).
Asunto(s)
Brucella/clasificación , Papio/microbiología , Filogenia , Animales , Técnicas de Tipificación Bacteriana , Brucella/genética , Brucella/aislamiento & purificación , ADN Bacteriano/genética , Femenino , Genes Bacterianos , Datos de Secuencia Molecular , Tipificación de Secuencias Multilocus , Hibridación de Ácido Nucleico , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADNRESUMEN
BACKGROUND: Endometrial and cervical polyps are masses of endometrium or cervical epithelium that bulge into the uterine or cervical lumen. The physiopathology and contributing factors of endometrial polyps development are still unknown. METHODS: Clinical and pathology records of 28 non-human primates with histologically confirmed endometrial and cervical polyps were reviewed. Twenty-one baboons with endometrial polyps were evaluated for age at diagnosis, body weight, menstrual cycle length, presence of endometriosis and adenomyosis and number of offspring, cesarean sections, and stillbirths. RESULTS: Endometrial polyps in baboons were associated with increased age, decreased menstrual cycle lengths, endometriosis, and decreased parity. No differences were found for weight, adenomyosis, or number of cesarean sections or stillbirths. CONCLUSIONS: Baboons are a promising model for the study of endometrial polyps because of their similarity to humans in both the development of endometrial polyps and association of many of the same risk factors.
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Enfermedades de los Monos/patología , Pólipos/veterinaria , Enfermedades del Cuello del Útero/veterinaria , Enfermedades Uterinas/veterinaria , Animales , Callithrix , Femenino , Macaca fascicularis , Papio , Pólipos/patología , Enfermedades del Cuello del Útero/patología , Enfermedades Uterinas/patologíaRESUMEN
The hypothalamic-pituitary-adrenal (HPA) axis plays a major role in the communication between the immune and neuroendocrine systems. Glucocorticoids are potent immunomodulatory hormones. In the present study, we evaluated the effect of three weekly courses of betamethasone, administered to pregnant baboons at 0.6, 0.65, and 0.7 of gestation, on maternal hematological parameters during treatment, maternal and fetal hematological parameters and lymphocyte populations at 0.95 of gestation, and fetal lymphoid organs and placental structure. Each weekly betamethasone course resulted in decreased granulocytes and increased lymphocytes and monocytes in maternal circulation (by percentage, p < 0.05). The percentage and absolute number of CD8+ T-cells in the maternal circulation were lower and CD4+ T-cells higher (p < 0.05) in treated pregnant animals at 0.95 gestation. The percentage of proliferating CD3- CD8+ cells was lower in blood obtained from the fetal heart of betamethasone-treated animals. In the betamethasone group, the number of CD8+ T-cells and NK cells were elevated and the number of T and CD4+ T-cells were reduced in fetal heart blood compared with the umbilical vein blood. The number of placental macrophages (CD68+ cells) per visual field in betamethasone-treated and control animals were not different. Taken together, our data show that betamethasone treatment of pregnant females with no indication of preterm labor affects some components of the fetal and maternal immune system, altering the maternal CD4+/CD8+ ratio and absolute number of fetal NK cell and maternal CD8+ T-cell.
Asunto(s)
Antiinflamatorios/efectos adversos , Betametasona/efectos adversos , Proliferación Celular/efectos de los fármacos , Feto/inmunología , Leucocitos Mononucleares/inmunología , Placenta/inmunología , Animales , Antiinflamatorios/administración & dosificación , Betametasona/administración & dosificación , Relación CD4-CD8 , Femenino , Feto/irrigación sanguínea , Feto/patología , Edad Gestacional , Papio , Placenta/irrigación sanguínea , Placenta/patología , EmbarazoRESUMEN
BACKGROUND: Coccidioidomycosis is an endemic fungal infection found most commonly in the Southwestern United States, Northwestern Mexico, and parts of Central and South America. Although infection is relatively uncommon during pregnancy, it is imperative to have an index of suspicion in order to diagnose and begin timely treatment to prevent dissemination and dire consequences. CASE REPORT: A 33-year-old Hispanic female was evaluated after she was involved in an automobile accident. Radiographic evaluation showed a 3.2 × 3.2 cm cavitary thick-walled lesion. A biopsy was negative for malignancy. Evaluation was positive for coccidioidomycosis by complement fixation reaction. Four months later, the patient presented 7 weeks into a pregnancy with massive hemoptysis. Bronchoscopy revealed bleeding from the right upper lobe and emergency embolization was performed. The patient had a spontaneous abortion 9 days after admission. The right upper and middle lobes of the lung were resected due to continuous bleeding. A subsequent pregnancy was un-eventful. Coccidioidomycosis titers remained negative throughout the second pregnancy. DISCUSSION: This case demonstrates the potential for severe pulmonary coccidioidomycosis and vascular strain of pregnancy-associated vascular expansion in the first trimester of pregnancy and the possibility of a favorable pregnancy outcome in subsequent pregnancies after appropriate treatment. The route of feto-maternal transmission and placental lesions in coccidioidomycosis are discussed.
RESUMEN
INTRODUCTION: The consumption of marijuana (exogenous cannabinoid) almost doubled in adults during last decade. Consumption of exogenous cannabinoids interferes with the endogenous cannabinoid (or "endocannabinoid" (eCB)) system (ECS), which comprises N-arachidonylethanolamide (anandamide, AEA), 2-arachidonoyl glycerol (2-AG), endocannabinoid receptors (cannabinoid receptors 1 and 2 (CB1R and CB2R), encoded by CNR1 and CNR2, respectively), and synthesizing/degrading enzymes (FAAH, fatty-acid amide hydrolase; MAGL, monoacylglycerol lipase; DAGL-α, diacylglycerol lipase-alpha). Reports regarding the toxic and therapeutic effects of pharmacological compounds targeting the ECS are sometimes contradictory. This may be caused by the fact that structure of the eCBs varies in the species studied. OBJECTIVES: First: to clone and characterize the cDNAs of selected members of ECS in a non-human primate (baboon, Papio spp.), and second: to compare those cDNA sequences to known human structural variants (single nucleotide polymorphisms and haplotypes). MATERIALS AND METHODS: Polymerase chain reaction-amplified gene products from baboon tissues were transformed into Escherichia coli. Amplicon-positive clones were sequenced, and the obtained sequences were conceptually translated into amino-acid sequences using the genetic code. RESULTS: Among the ECS members, CNR1 was the best conserved gene between humans and baboons. The phenotypes associated with mutations in the untranslated regions of this gene in humans have not been described in baboons. One difference in the structure of CNR2 between humans and baboons was detected in the region with the only known clinically relevant polymorphism in a human receptor. All of the differences in the amino-acid structure of DAGL-α between humans and baboons were located in the hydroxylase domain, close to phosphorylation sites. None of the differences in the amino-acid structure of MAGL observed between baboons and humans were located in the area critical for enzyme function. CONCLUSION: The evaluation of the data, obtained in non-human primate model of cannabis-related developmental exposure should take into consideration possible evolutionary-determined species-specific differences in the CB1R expression, CB2R transduction pathway, and FAAH and DAGLα substrate-enzyme interactions.
Asunto(s)
Endocannabinoides/genética , Modelos Animales , Investigación Biomédica Traslacional , Amidohidrolasas/genética , Animales , Humanos , Lipoproteína Lipasa/genética , Hígado/metabolismo , Monoacilglicerol Lipasas/genética , Papio , Receptor Cannabinoide CB1/genética , Receptor Cannabinoide CB2/genética , Especificidad de la EspecieRESUMEN
Obesity is associated with vitamin D deficiency, which can lead to serious problems during pregnancy. However, the mechanisms of the deficiency and guidelines for vitamin D supplementation during pregnancy are not established yet, and variations in environmental exposures combined with the difficulties of performing research in pregnant women are obstacles in the evaluation of vitamin D metabolism. Baboons (Papio spp.) are an excellent, well-established model for reproductive research and represent a unique opportunity to study vitamin D metabolism in a controlled environment. This study used secondary data and specimen analysis as well as a novel experimental design to evaluate pregnant and nonpregnant baboons that were or were not exposed to sunlight while they were obese and after weight reduction. Daily D3 intake was 71% higher in nonpregnant obese baboons than in their nonobese counterparts, but serum vitamin D concentrations did not differ between these populations. In addition, serum 25-hydroxyvitamin D concentrations correlated negatively with the obesity index. This report is the first to show the effect of obesity and pregnancy on vitamin D concentrations in a NHP population. These data underline the importance of adequate vitamin D supplementation in obese animals.
Asunto(s)
Obesidad/sangre , Papio , Preñez/sangre , Deficiencia de Vitamina D , Vitamina D/análogos & derivados , Vitaminas/sangre , Animales , Femenino , Vivienda para Animales , Humanos , Modelos Animales , Embarazo , Vitamina D/sangre , Vitamina D/uso terapéutico , Deficiencia de Vitamina D/sangre , Vitaminas/uso terapéuticoRESUMEN
BACKGROUND: Chemerin, encoded by the retinoic acid receptor responder 2 (RARRES2) gene is an adipocytesecreted protein with autocrine/paracrine functions in adipose tissue, metabolism and inflammation with a recently described function in vascular tone regulation, liver, steatosis, etc. This molecule is believed to represent a critical endocrine signal linking obesity to diabetes. There are no data available regarding evolution of RARRES2 in non-human primates and great apes. Expression profile and orthology in RARRES2 genes are unknown aspects in the biology of this multigene family in primates. Thus; we attempt to describe expression profile and phylogenetic relationship as complementary knowledge in the function of this gene in primates. To do that, we performed A RT-PCR from different tissues obtained during necropsies. Also we tested the hypotheses of positive evolution, purifying selection, and neutrality. And finally a phylogenetic analysis was made between primates RARRES2 protein. RESULTS: RARRES2 transcripts were present in liver, lung, adipose tissue, ovary, pancreas, heart, hypothalamus and pituitary tissues. Expression in kidney and leukocytes were not detectable in either species. It was determined that the studied genes are orthologous. CONCLUSIONS: RARRES2 evolution fits the hypothesis of purifying selection. Expression profiles of the RARRES2 gene are similar in baboons and chimpanzees and are also phylogenetically related.
Asunto(s)
Animales , Masculino , Femenino , Papio/genética , Pan troglodytes/genética , Receptores de Ácido Retinoico/genética , Evolución Molecular , Filogenia , Datos de Secuencia Molecular , Secuencia de Bases , Reacción en Cadena de la Polimerasa de Transcriptasa InversaRESUMEN
Despite that anatomists consider the cardia as a portion of the stomach, there is disagreement in the literature over whether the cardia mucosa, described as columnar-lined with mucus-producing glands (CLMMG) with or without occasional interspersed oxyntic cells, is part of the stomach, part of the esophagus or a distinct entity. For some authors this mucosa phenotype is a metaplastic glandular change of the distal esophagus caused by protracted gastro-esophageal reflux (GER). In this survey, the presence of CLMMG mucosa was searched for at the esophagus-gastric junction in 50 non-human primates (NHP). The length of the CLMMG (between the squamous epithelium of the esophagus and the first oxyntic fundic gastric gland) was assessed by the aid of an ocular microscale. In all three foetuses, all four stillborn baboons and one 4 day old baboon, the columnar-lined mucosa showed depressions that corresponded to early epithelial pits without glands. In the remaining 45 post-natal NHP, the length of the CLMMG mucosa varied from 0.8 mm to 25.2 mm, and the CLMMG mucosa had replaced the distal esophageal squamous epithelium. The size was neither influenced by the post-natal age nor by the gender of the animals. In NHP, regurgitation with rumination is a natural physiological process leading to GER. The present investigation substantiates the notion that the columnar-lined mucosa with mucus-producing glands is a post-natal developmental process in NHP. These animals seem to offer an excellent spontaneous model to study the series of histological events that take place in the distal esophagus of NHP, most likely under the influence of protracted GER.
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This study describes conventional and real-time polymerase chain reaction (PCR) methods developed to detect and quantify Trypanosoma cruzi DNA in cynomolgus monkeys (Macaca fascicularis) using formalin-fixed paraffin-embedded blocks archived for periods of up to 6 years. The highest concentration of T. cruzi DNA was found in the myocardium, urinary bladder, stomach, lymph node, adrenal gland, and colon. The concentration of T. cruzi DNA detected in cardiac tissues was 10-100-fold greater than found elsewhere; the mean concentrations of T. cruzi DNA in non-cardiac tissues were otherwise comparable. Trypanosoma cruzi DNA was amplified from cerebrum but not cerebellum or kidney. Successful use of DNA from formalin-fixed, paraffin-embedded blocks is important because most pathology laboratories routinely archive wax blocks. This archived resource can be used for further studies on the prevalence of this disease.
Asunto(s)
Bancos de Muestras Biológicas , Enfermedad de Chagas/parasitología , ADN Protozoario/aislamiento & purificación , Macaca fascicularis/parasitología , Reacción en Cadena de la Polimerasa , Trypanosoma cruzi/aislamiento & purificación , Animales , Enfermedad de Chagas/diagnóstico , Enfermedad de Chagas/patología , Enfermedad de Chagas/veterinaria , ADN Protozoario/genética , Femenino , Masculino , Estudios Retrospectivos , Manejo de Especímenes , Trypanosoma cruzi/genéticaRESUMEN
Knowledge of altered maternal nutrition effects on growth-regulating systems is critical to understanding normal and abnormal fetal development. There are many reports of hepatic fetal IGF system responses to maternal nutrient restriction (MNR) during pregnancy in rodents and sheep but none in nonhuman primates. We determined effects of MNR on the fetal baboon hepatic IGF system. Social groups of female baboons were fed ad libitum, controls, or 70% controls (MNR) from 0.16 to 0.5 gestation and fetuses delivered by cesarean section. Fetal liver tissue was analyzed for IGF-I, IGF-II, and IGF binding protein (IGFBP)-3 mRNA by in situ hybridization and quantitative RT-PCR and protein by immunohistochemistry (IHC); IGF-I receptor, IGF-II receptor by quantitative RT-PCR and IHC and IGFBP-1 by in situ hybridization and IHC. MNR did not alter fetal body or liver weight. Fetal hepatic glycogen staining increased with MNR. MNR reduced fetal hepatic IGF-I and IGF-II and increased IGFBP-1 mRNA and decreased IGF-I, IGF-II, IGF-I receptor, and IGF-II receptor protein and increased protein for IGFBP-1 and IGFBP-3. MNR increased caspase-3, indicating apoptosis and decreased Akt staining, indicating decreased nutrient sensing. In conclusion, whereas fetal body and liver weights did not change in response to moderate MNR during the first half of baboon pregnancy, the major indices of function of the hepatic IGF system measured were all reduced.
Asunto(s)
Feto/metabolismo , Hígado/metabolismo , Estado Nutricional , Embarazo/fisiología , Somatomedinas/metabolismo , Líquido Amniótico/metabolismo , Animales , Encéfalo/metabolismo , Caspasa 3/metabolismo , Femenino , Desarrollo Fetal , Proteína 1 de Unión a Factor de Crecimiento Similar a la Insulina/metabolismo , Proteína 3 de Unión a Factor de Crecimiento Similar a la Insulina/metabolismo , Antígeno Ki-67/metabolismo , Riñón/metabolismo , Hígado/crecimiento & desarrollo , Glucógeno Hepático/metabolismo , Tamaño de los Órganos , Papio , Placenta/metabolismo , Placentación , Proteínas Proto-Oncogénicas c-akt/metabolismo , ARN Mensajero/metabolismo , Receptor IGF Tipo 1/metabolismo , Receptor IGF Tipo 2/metabolismo , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Somatomedinas/genéticaRESUMEN
BACKGROUND: Maternal obesity represents a risk factor for pregnancy-related complications. Glucocorticoids are known to promote obesity in adults. METHODS: We evaluated maternal and fetal metabolic changes during and after 3 weekly courses of betamethasone administered to pregnant baboons (Papio subspecies) at doses equivalent to those given to pregnant women. RESULTS: Betamethasone administration during the second half of pregnancy increased maternal weight but neither maternal food intake nor fetal weight, as assessed at the end of gestation. Betamethasone increased maternal serum glucose concentration, the ratio of insulin-like growth factor-I and insulin-like growth factor binding protein-3, and serum leptin during treatment (normalized by 17, 35, and 45 days posttreatment, respectively, for each parameter). Maternal and fetal serum leptin concentrations did not differ between groups at the end of gestation. CONCLUSION: Prolonged maternal hyperleptinemia caused by betamethasone administration in the second half of gestation did not change fetal metabolic parameters measured and placental leptin distribution at the end of gestation.
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Betametasona/administración & dosificación , Sangre Fetal/metabolismo , Feto/efectos de los fármacos , Glucocorticoides/administración & dosificación , Proteína 3 de Unión a Factor de Crecimiento Similar a la Insulina/sangre , Factor I del Crecimiento Similar a la Insulina/metabolismo , Leptina/sangre , Animales , Betametasona/metabolismo , Glucemia/efectos de los fármacos , Peso Corporal/efectos de los fármacos , Cesárea , Colesterol/sangre , Esquema de Medicación , Ingestión de Alimentos/efectos de los fármacos , Femenino , Peso Fetal/efectos de los fármacos , Feto/metabolismo , Edad Gestacional , Glucocorticoides/metabolismo , Hidrocortisona/sangre , Inyecciones Intramusculares , Factor II del Crecimiento Similar a la Insulina/metabolismo , Intercambio Materno-Fetal/efectos de los fármacos , Papio cynocephalus , Placenta/efectos de los fármacos , Placenta/metabolismo , EmbarazoRESUMEN
BACKGROUND: Brucellosis is veterinary and human health problem. METHODS: A 13-year-old wild caught multiparous and an 8-year-old colony-born nulliparous baboon had stillbirths in the second trimester of pregnancy. Culture isolates from both postpartum uteruses were characterized using traditional biochemical analysis, PCR, and multilocus sequencing. RESULTS: The isolates morphologically resembled Brucella although their phenotypic characteristics were not consistent with any currently described species. The isolates represent a novel lineage within the genus with unique alleles, not previously seen in surveys of greater than 300 isolates representing the known diversity of the genus, present at 5/9 loci examined. CONCLUSIONS: The described cases are to the best of our knowledge the first presentation of a naturally acquired Brucella infection in non-human primates associated with stillbirths from the same colony where Brucella seropositivity in the baboons was described 45 years ago. The organism appears to represent a previously undescribed Brucella species.
Asunto(s)
Brucella/aislamiento & purificación , Brucelosis/veterinaria , Muerte Fetal/veterinaria , Papio/microbiología , Mortinato/veterinaria , Animales , Brucella/genética , Brucelosis/complicaciones , Brucelosis/microbiología , Femenino , Muerte Fetal/microbiología , Genotipo , EmbarazoRESUMEN
BACKGROUND: Stillbirths in non-human primates are a major problem and represent failure of the maternal-fetal-placental unit to maintain normal relationships because of various endogenous, undetermined or environmental factors. METHODS: Records of 236 stillborns and their dams in a Macaca fascicularis colony during a 7-year period were reviewed retrospectively. RESULTS: The 7-year stillbirth incidence was 11.99% (236 stillbirths, 1967 live births). Most (61.02%, n = 144) were of undetermined etiology. Fetal causes included trauma (22.46%, n = 53), fetal pneumonia (0.85%, n = 2) and congenital anomalies (0.42%, n = 1). Maternal causes included dystocia (9.75%, n = 23) and uterine rupture (0.42%, n = 1). Forty-nine placentas were available for histologic evaluation; there was placentitis in five, necrosis in five and placental abruption in two. Most stillbirths occurred close to term. First stillbirths usually occurred in 8- to 12-year-old animals during the first six pregnancies. CONCLUSIONS: Most stillbirths were of undetermined etiology. Fetal trauma was the most common cause.
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Macaca fascicularis , Mortinato/veterinaria , Animales , Femenino , Incidencia , Masculino , Embarazo , Factores de Riesgo , Mortinato/epidemiologíaRESUMEN
BACKGROUND: Congenital transmission of Trypanosoma cruzi has been described in humans and experimental work has been conducted with mice, but not with non-human primates (NHPs). METHODS: We conducted a retrospective study of female baboons (Papio hamadryas spp.) naturally seropositive or seronegative for T. cruzi with history of fetal loss, and we report a stillbirth in a cynomolgus macaque (Macaca fascicularis) with placental T. cruzi amastigotes. RESULTS: There were no differences in menstrual cycle parameters and the number of fetal losses between seropositive and seronegative baboons with history of fetal loss. The amount of parasite DNA detected using quantitative polymerase chain reaction (Q-PCR) in M. fascicularis placenta was within the range detected in infected baboon tissues. CONCLUSIONS: There is no evidence that chronic maternal T. cruzi infection causes fetal loss in baboons. Q-PCR is a useful diagnostic tool to study archived NHP placentas.
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Enfermedades del Simio Antropoideo/parasitología , Enfermedad de Chagas/veterinaria , Papio , Mortinato/veterinaria , Trypanosoma cruzi/crecimiento & desarrollo , Animales , Enfermedad de Chagas/parasitología , ADN Protozoario/química , ADN Protozoario/genética , Resultado Fatal , Femenino , Inmunohistoquímica , Macaca fascicularis , Masculino , Placenta/parasitología , Reacción en Cadena de la Polimerasa/veterinaria , Embarazo , Estudios RetrospectivosRESUMEN
BACKGROUND: In baboons, Papio sp. neoplasms tend to affect the hematopoietic system most commonly, with rare documentation of myxomatous neoplasms. In contrast, women can develop myxomatous masses within deep peripelvic tissues with some frequency during their reproductive years. METHODS: We have identified and examined, retrospectively, myxomatous perineal masses in twelve female baboons within one research facility and compared their histopathologic, immunohistochemical and electron microscopic features to their human variants. RESULTS: Our results indicate that these myxomatous neoplasms, in humans and non-human primates, share common features. CONCLUSION: Further research, particularly molecular genetic analysis, may be needed to identify the baboon as a true animal model for myxomatous perineal neoplasms.
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Enfermedades de los Monos/patología , Mixoma/veterinaria , Papio , Neoplasias Pélvicas/veterinaria , Perineo/patología , Animales , Femenino , Inmunohistoquímica/veterinaria , Microscopía Electrónica/veterinaria , Mixoma/patología , Mixoma/ultraestructura , Neoplasias Pélvicas/patología , Neoplasias Pélvicas/ultraestructura , Estudios RetrospectivosRESUMEN
BACKGROUND: Several risk factors are associated with the incidence of human stillbirths. The prevention of stillbirths in women is a pressing clinical problem. METHODS: We reviewed 402 pathology records of fetal loss occurring in a large baboon (Papio spp.) colony during a 15-year period. Clinical histories of 565 female baboons with one or more fetal losses during a 20-year period were analyzed for weight, age, and reproductive history. RESULTS: Fetal loss was most common at term (35.57%) and preterm (28.61%) and less common in the first half of gestation (11.20%) and post-term (5.22%). Greater maternal weight, older age, history of stillbirth and higher parity were independent predictors for stillbirth. An exponential increase in the incidence of fetal loss was observed beginning at age 14 years in baboons. CONCLUSIONS: Fetal loss and maternal risk factors associated with stillbirths in baboons were similar to those documented in women.
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Enfermedades del Simio Antropoideo/patología , Papio hamadryas , Mortinato/veterinaria , Animales , Enfermedades del Simio Antropoideo/epidemiología , Peso Corporal , Modelos Animales de Enfermedad , Femenino , Edad Gestacional , Edad Materna , Embarazo , Análisis de Regresión , Historia Reproductiva , Estudios Retrospectivos , Factores de Riesgo , Mortinato/epidemiologíaRESUMEN
Developmental programming is defined as the process by which gene-environment interaction in the developing organism leads to permanent changes in phenotype and function. Numerous reports of maternal nutrient restriction during pregnancy demonstrate altered renal development. Typically this alteration manifests as a reduction in the total number of glomeruli in the mature kidney of the offspring, and suggests that predisposition to develop chronic renal disease may include an in utero origin. In a previous study, we defined the transcriptome in the kidney from fetuses of control (CON, fed ad libitum) and nutrient-restricted (NR, fed 70% of CON starting at 0.16 gestation (G)) pregnancies at half-way through gestation (0.5G), and established transcriptome and morphological changes in NR kidneys compared to CON. One goal of the present study was to use transcriptome data from fetal kidneys of CON and NR mothers at 0.5G with histological data to identify the molecular mechanisms that may regulate renal development. A second goal was to identify mechanisms by which NR elicits its affect on fetal baboon kidney. We have used an end-of-pathway gene expression analysis to prioritize and identify key pathways regulating the 0.5G kidney phenotype in response NR. From these data we have determined that the mammalian target of rapamycin (mTOR) signalling pathway is central to this phenotype.
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Regulación del Desarrollo de la Expresión Génica , Riñón/embriología , Riñón/fisiología , Fenómenos Fisiologicos Nutricionales Maternos/genética , Proteínas Quinasas/genética , Alimentación Animal , Fenómenos Fisiológicos Nutricionales de los Animales/genética , Animales , Restricción Calórica , Femenino , Genómica , Inmunohistoquímica , Papio , Fenotipo , Embarazo , Proteínas Quinasas/metabolismo , Transducción de Señal/genética , Serina-Treonina Quinasas TOR , Transcripción Genética , Factor A de Crecimiento Endotelial Vascular/genética , Factor A de Crecimiento Endotelial Vascular/metabolismoRESUMEN
Trisomy 13 in humans is the third most common autosomal abnormality at birth, after trisomy 21 and trisomy 18. It has a reported incidence of between 1:5,000 and 1:30,000 live births. It is associated with multiple abnormalities, many of which shorten lifespan. We describe here the first reported case of a baboon (Papio hamadryas) with trisomy of chromosome 17, which is homologous to human chromosome 13. The trisomic infant was born to a consanguineous pair of baboons and had morphological characteristics similar to those observed in human trisomy 13, including bilateral polydactyly in the upper limbs, a patent foramen ovale, and pyelectasis. Molecular DNA analysis using human chromosome 13 markers was consistent with the affected infant inheriting two copies of chromosome 17 derived from the same parental chromosome. This trisomy was, therefore, due to either an error in meiosis II or the result of postzygotic nondisjunction. The parental origin, however, could not be determined.
Asunto(s)
Cromosomas de los Mamíferos/genética , Defectos del Tabique Interatrial/genética , Pelvis Renal/anomalías , Papio hamadryas/anomalías , Polidactilia/genética , Trisomía/diagnóstico , Animales , Brazo/patología , Dilatación Patológica/genética , Femenino , Atrios Cardíacos/patología , Defectos del Tabique Interatrial/patología , Humanos , Endogamia , Pelvis Renal/patología , Masculino , Papio hamadryas/genética , Polidactilia/patología , Trisomía/genéticaRESUMEN
Reduced food availability in pregnancy influences fetal growth, obstetric outcomes and offspring health in both developing and developed countries. The objective of the present study was to determine responses to moderate global maternal nutrient restriction (MNR) during pregnancy in baboons (Papio hamadryas) - an established non-human primate model for pregnancy-related research. Starting at 30 d gestation (dG), twelve pregnant baboons received 70 % of food (MNR group) consumed by twenty ad libitum-fed pregnant controls. Maternal body weight, BMI, food intake and physical activity were measured before pregnancy, at 90 dG and at 165 dG (full-term 180 dG). Fetal and placental weights were recorded at the time of Caesarean section (90 and 165 dG). Activity patterns were also evaluated in fourteen non-pregnant female baboons. Behavioural observations were made in five non-pregnant, six control and four MNR animals. Pregnant baboons decreased overall physical activity and energy-expensive behaviours compared with non-pregnant baboons. In the MNR group, maternal weight, weight gain and maternal physical activity were reduced compared with the control animals. MNR decreased placental weight and volume compared with control, while fetal weight and length were unaffected. We conclude that decreased physical activity and increased usage of maternal available body stores play an important role in the maternal response to pregnancy. Also, adaptations in maternal behaviour and energy utilisation protect fetal growth during moderate MNR.