Geographical ancestry is a key determinant of epidermal morphology and dermal composition.

BACKGROUND: Geographical ancestry plays a key role in determining the susceptibility of human skin to external insults and dermatological disease. Despite this, studies of skin from individuals of diverse geographical ancestry focus primarily on epidermal pigmentation. Few reports characterize the gross morphology and composition of the dermis and dermal-epidermal junction (DEJ). OBJECTIVES: To characterize epidermal morphology and dermal composition in skin from individuals of diverse geographical ancestry. METHODS: Immunohistochemical techniques were used to assess epidermal morphology and protein composition of the DEJ and dermal extracellular matrix in photoprotected skin from young African, Eurasian and Far East Asian individuals (n = 7 per group; age 18-30 years). RESULTS: The epidermis of African skin was thicker, with deeper rete ridges and a more convoluted DEJ than Eurasian and Far East Asian skin. Compared with Eurasians, protein composition of the DEJ was collagen VII poor in African and Far East Asian skin (P < 0·001 and P < 0·01, respectively); the dermis of African skin was enriched in fibrillar collagens (P < 0·05), but was relatively elastin poor (P < 0·05). African dermis was abundant in fibrillin-rich microfibrils and fibulin-5 (P < 0·001 and P < 0·001, respectively) compared with Eurasian and Far East Asian skin. CONCLUSIONS: We demonstrate that fundamental differences exist in skin structure and composition in individuals of diverse geographical ancestry. Disparate environmental pressures encountered by ancestral human populations living at different latitudes may have driven adaptations in skin structure and composition. Further research into the functional significance and clinical consequences of these differences is warranted.

Archaeopteryx feathers and bone chemistry fully revealed via synchrotron imaging.

Evolution of flight in maniraptoran dinosaurs is marked by the acquisition of distinct avian characters, such as feathers, as seen in Archaeopteryx from the Solnhofen limestone. These rare fossils were pivotal in confirming the dinosauria-avian lineage. One of the key derived avian characters is the possession of feathers, details of which were remarkably preserved in the Lagerstätte environment. These structures were previously simply assumed to be impressions; however, a detailed chemical analysis has, until now, never been completed on any Archaeopteryx specimen. Here we present chemical imaging via synchrotron rapid scanning X-ray fluorescence (SRS-XRF) of the Thermopolis Archaeopteryx, which shows that portions of the feathers are not impressions but are in fact remnant body fossil structures, maintaining elemental compositions that are completely different from the embedding geological matrix. Our results indicate phosphorous and sulfur retention in soft tissue as well as trace metal (Zn and Cu) retention in bone. Other previously unknown chemical details of Archaeopteryx are also revealed in this study including: bone chemistry, taphonomy (fossilization process), and curation artifacts. SRS-XRF represents a major advancement in the study of the life chemistry and fossilization processes of Archaeopteryx and other extinct organisms because it is now practical to image the chemistry of large specimens rapidly at concentration levels of parts per million. This technique has wider application to the archaeological, forensic, and biological sciences, enabling the mapping of "unseen" compounds critical to understanding biological structures, modes of preservation, and environmental context.

Exploring diagonal gait using a forward dynamic three-dimensional chimpanzee simulation.

Primates are unusual among terrestrial quadrupedal mammals in that at walking speeds they prefer diagonal rather than lateral gaits. A number of reasons have been proposed for this preference in relation to the arboreal ancestry of modern primates: stability, energetic cost, neural control, skeletal loading, and limb interference avoiding. However, this is a difficult question to explore experimentally since most primates only occasionally use anything other than diagonal gaits. An alternative approach is to produce biologically realistic computer simulations of primate gait that enable the constraints of biomechanical loading and the energetics of different modes of locomotion to be explored. In this paper we describe such a model for the chimpanzee Pan troglodytes. The simulation is able to produce spontaneous quadrupedal locomotion, and the footfall sequences generated are split between lateral and diagonal footfall sequences with no obvious energetic benefit associated with either option. However, out of 10 successful simulation runs, 5 were lateral sequence/lateral couplet gaits indicating a preference for a specific lateral footfall sequence with a relatively tightly constrained phase difference between the fore- and hindlimbs. This suggests that the choice of diagonal walking gaits in chimpanzees is not a simple mechanical phenomenon and that diagonal walking gaits in primates are selected for by multiple factors.

Minimum convex hull mass estimations of complete mounted skeletons.

Body mass is a critical parameter used to constrain biomechanical and physiological traits of organisms. Volumetric methods are becoming more common as techniques for estimating the body masses of fossil vertebrates. However, they are often accused of excessive subjective input when estimating the thickness of missing soft tissue. Here, we demonstrate an alternative approach where a minimum convex hull is derived mathematically from the point cloud generated by laser-scanning mounted skeletons. This has the advantage of requiring minimal user intervention and is thus more objective and far quicker. We test this method on 14 relatively large-bodied mammalian skeletons and demonstrate that it consistently underestimates body mass by 21 per cent with minimal scatter around the regression line. We therefore suggest that it is a robust method of estimating body mass where a mounted skeletal reconstruction is available and demonstrate its usage to predict the body mass of one of the largest, relatively complete sauropod dinosaurs: Giraffatitan brancai (previously Brachiosaurus) as 23200 kg.

Infrared mapping resolves soft tissue preservation in 50 million year-old reptile skin.

Non-destructive Fourier Transform InfraRed (FTIR) mapping of Eocene aged fossil reptile skin shows that biological control on the distribution of endogenous organic components within fossilized soft tissue can be resolved. Mapped organic functional units within this approximately 50 Myr old specimen from the Green River Formation (USA) include amide and sulphur compounds. These compounds are most probably derived from the original beta keratin present in the skin because fossil leaf- and other non-skin-derived organic matter from the same geological formation do not show intense amide or thiol absorption bands. Maps and spectra from the fossil are directly comparable to extant reptile skin. Furthermore, infrared results are corroborated by several additional quantitative methods including Synchrotron Rapid Scanning X-Ray Fluorescence (SRS-XRF) and Pyrolysis-Gas Chromatography/Mass Spectrometry (Py-GC/MS). All results combine to clearly show that the organic compound inventory of the fossil skin is different from the embedding sedimentary matrix and fossil plant material. A new taphonomic model involving ternary complexation between keratin-derived organic molecules, divalent trace metals and silicate surfaces is presented to explain the survival of the observed compounds. X-ray diffraction shows that suitable minerals for complex formation are present. Previously, this study would only have been possible with major destructive sampling. Non-destructive FTIR imaging methods are thus shown to be a valuable tool for understanding the taphonomy of high-fidelity preservation, and furthermore, may provide insight into the biochemistry of extinct organisms.

The kinematics of load carrying in humans and great apes: implications for the evolution of human bipedalism.

We present a comparison of loaded and unloaded carrying kinematics in humans, common chimpanzees (Pan troglodytes), bonobos (Pan paniscus), western lowland gorillas (Gorilla gorilla gorilla) and Bornean and Sumatran orang-utans (Pongo pygmaeus and Pongo abelii). Human hindlimb joint and segment angles were collected during treadmill locomotion using infrared motion analysis cameras. Non-human primate fore- and hindlimb joint and segment angles were collected at zoos during free-ranging locomotion using a standard video camera. In quadrupedal locomotion there were small but potentially important changes associated with load carriage leading to a more upright trunk and a shift in shoulder excursion. These changes were exacerbated as locomotion shifts from quadrupedal to tripedal and bipedal gaits when carrying more awkward loads suggesting a possible adaptive sequence. However, food carrying may favour a highly flexed bent-hip bent-knee bipedal gait since it allows simultaneous foraging and hoarding. In bipedal humans no changes in limb kinematics were seen associated with type of load, although asymmetric loads may lead to lateral postural shifts. Carrying may therefore be an important component of the evolutionary shift to habitual bipedalism, although further work is needed to understand the full biomechanical implications.

Modelling temperature and humidity effects on web performance: implications for predicting orb-web spider (Argiope spp.) foraging under Australian climate change scenarios.

Phenotypic features extending beyond the body, or EPs, may vary plastically across environments. EP constructs, such as spider webs, vary in property across environments as a result of changes to the physiology of the animal or interactions between the environment and the integrity of the material from which the EP is manufactured. Due to the complexity of the interactions between EP constructs and the environment, the impact of climate change on EP functional integrity is poorly understood. Here we used a dynamic model to assess how temperature and humidity influence spider web major ampullate (MA) silk properties. MA silk is the silk that absorbs the impact of prey striking the web, hence our model provides a useful interpretation of web performance over the temperature (i.e. 20-55°C) and humidity (i.e. 15-100%) ranges assessed. Our results showed that extremely high or low humidity had direct negative effects on web capture performance, with changes in temperature likely having indirect effects. Undeniably, the effect of temperature on web architecture and its interactive effect with humidity on web tension and capture thread stickiness need to be factored into any further predictions of plausible climate change impacts. Since our study is the first to model plasticity in an EP construct's functionality and to extrapolate the results to predict climate change impacts, it stands as a template for future studies that endeavour to make predictions about the influence of climate change on animal EPs.

A pan-neotropical analysis of hunting preferences.

Hunting in the neotropics is a widespread form of resource extraction. However, there is increasing concern that current activities are leading to the decline and extirpation of vulnerable species; particulary ateline primates, large ungulates (such as tapirs and white-lipped peccaries) and large birds such as curassows. Hunting patterns are expected to be a product of two principal influences: the value of return for a given amount of effort invested into hunting, and cultural factors that determine the prestige and usefulness of prey. Previous work has suggested that hunting profiles change in a predictable way over time, becoming more diverse and more dependent on smaller bodied species as preferred, large-bodied prey become scarcer. In this paper, we evaluate the hunting profiles of 78 neotropical communities in Central and South America. We investigate the uniformity of species preferences, whether communities that are geographically closer have similar hunting profiles, and whether the age and size of settlements can be used to predict the type and diversity of species targeted. We found that there was only a weak correlation between the structure of communities' hunting profiles and their geographical proximity. Neither a community's size nor age was a good predictor of the shape and structure of its hunting profile. Our data suggest that either the availability of prey or the cultural influences dictating the value of different species can change rapidly over small distances, and that older and larger settlements do not impact prey species distributions in a predictable way.

Human bipedal instability in tree canopy environments is reduced by "light touch" fingertip support.

Whether tree canopy habitats played a sustained role in the ecology of ancestral bipedal hominins is unresolved. Some argue that arboreal bipedalism was prohibitively risky for hominins whose increasingly modern anatomy prevented them from gripping branches with their feet. Balancing on two legs is indeed challenging for humans under optimal conditions let alone in forest canopy, which is physically and visually highly dynamic. Here we quantify the impact of forest canopy characteristics on postural stability in humans. Viewing a movie of swaying branches while standing on a branch-like bouncy springboard destabilised the participants as much as wearing a blindfold. However "light touch", a sensorimotor strategy based on light fingertip support, significantly enhanced their balance and lowered their thigh muscle activity by up to 30%. This demonstrates how a light touch strategy could have been central to our ancestor's ability to avoid falls and reduce the mechanical and metabolic cost of arboreal feeding and movement. Our results may also indicate that some adaptations in the hand that facilitated continued access to forest canopy may have complemented, rather than opposed, adaptations that facilitated precise manipulation and tool use.

Energetic efficiency and ecology as selective factors in the saltatory adaptation of prosimian primates.

We tend to assume that natural selection will bring about 'optimal' configurations in morphology and behaviour. Jumping locomotion involves large forces and energy costs which, in this non-cyclic activity, are generated anew with each jump. Jumping appears to be, therefore, a major target for optimization. It has been a standard assumption that jumpers will tend to adopt ballistic paths which will minimize the energy costs involved in jumping, and will act to minimize the loads applied to the body. Experimental studies, using kinematic analysis of digitized video recordings of the jump in five prosimian primates, with a 25-fold range in body mass, show that most do not adopt energy-efficient paths until the length of the jump is close to the maximum they can attain. Statistical analysis of quantified field observations suggests that, of three primate jumpers, only the largest, most unspecialized appears to take the forces applied to the musculoskeletal system into consideration when selecting supports used in locomotion. 'Ecological' factors, such as time pressure and habitat support density, may thus be the prime consideration for many species in determining the manner in which they jump.

Leaf metallome preserved over 50 million years.

Large-scale Synchrotron Rapid Scanning X-ray Fluorescence (SRS-XRF) elemental mapping and X-ray absorption spectroscopy are applied here to fossil leaf material from the 50 Mya Green River Formation (USA) in order to improve our understanding of the chemistry of fossilized plant remains. SRS-XRF of fossilized animals has previously shown that bioaccumulated trace metals and sulfur compounds may be preserved in their original distributions and these elements can also act as biomarkers for specific biosynthetic pathways. Similar spatially resolved chemical data for fossilized plants is sparsely represented in the literature despite the multitude of other chemical studies performed. Here, synchrotron data from multiple specimens consistently show that fossil leaves possess chemical inventories consisting of organometallic and organosulfur compounds that: (1) map discretely within the fossils, (2) resolve fine scale biological structures, and (3) are distinct from embedding sedimentary matrices. Additionally, the chemical distributions in fossil leaves are directly comparable to those of extant leaves. This evidence strongly suggests that a significant fraction of the chemical inventory of the examined fossil leaf material is derived from the living organisms and that original bioaccumulated elements have been preserved in situ for 50 million years. Chemical information of this kind has so far been unknown for fossilized plants and could for the first time allow the metallome of extinct flora to be studied.

Trace metals as biomarkers for eumelanin pigment in the fossil record.

Well-preserved fossils of pivotal early bird and nonavian theropod species have provided unequivocal evidence for feathers and/or downlike integuments. Recent studies have reconstructed color on the basis of melanosome structure; however, the chemistry of these proposed melanosomes has remained unknown. We applied synchrotron x-ray techniques to several fossil and extant organisms, including Confuciusornis sanctus, in order to map and characterize possible chemical residues of melanin pigments. Results show that trace metals, such as copper, are present in fossils as organometallic compounds most likely derived from original eumelanin. The distribution of these compounds provides a long-lived biomarker of melanin presence and density within a range of fossilized organisms. Metal zoning patterns may be preserved long after melanosome structures have been destroyed.

The energetic costs of load-carrying and the evolution of bipedalism.

The evolution of habitual bipedalism is still a fundamental yet unsolved question for paleoanthropologists, and carrying is popular as an explanation for both the early adoption of upright walking and as a positive selection pressure once a terrestrial lifestyle had been adopted. However, to support or reject any hypothesis that suggests carrying efficiency was an important selective pressure, we need quantitative data on the costs of different forms of carrying behavior, especially infant-carrying since reduction in the grasping capabilities of the foot would have prevented infants from clinging on for long durations. In this study, we tested the hypothesis that the mode of load carriage influences the energetic cost of locomotion. Oxygen consumption was measured in seven female participants walking at a constant speed while carrying four different 10-kg loads (a weighted vest, 5-kg dumbbells carried in each hand, a mannequin infant carried on one hip, and a 10-kg dumbbell carried in a single hand). Oxygen consumption was also measured during unloaded standing and unloaded walking. The results show that the weighted vest requires the least amount of energy of the four types of carrying and that, for this condition, humans are as efficient as mammals in general. The balanced load was carried with approximately the predicted energy cost. However, the asymmetrical conditions were considerably less efficient, indicating that, unless infant-carrying was the adaptive response to a strong environmental selection pressure, this behavior is unlikely to have been the precursor to the evolution of bipedalism.

An agent-based model of group decision making in baboons.

We present an agent-based model of the key activities of a troop of chacma baboons (Papio hamadryas ursinus) based on the data collected at De Hoop Nature Reserve in South Africa. We analyse the predictions of the model in terms of how well it is able to duplicate the observed activity patterns of the animals and the relationship between the parameters that control the agent's decision procedure and the model's predictions. At the current stage of model development, we are able to show that across a wide range of decision parameter values, the baboons are able to achieve their energetic and social time requirements. The simulation results also show that decisions concerning movement (group action selection) have the greatest influence on the outcomes. Those cases where the model's predictions fail to agree with the observed activity patterns have highlighted key elements that were missing from the field data, and that would need to be collected in subsequent fieldwork. Based on our experience, we believe group decision making is a fertile field for future research, and agent-based modelling offers considerable scope for understanding group action selection.

A biomechanical investigation into the absence of leaping in the locomotor repertoire of the slender loris (Loris tardigradus).

Unlike all other primates, members of the subfamily Lorisinae are never seen to leap. To investigate the anatomical specializations that are behind the absence of leaping in their locomotor repertoire, a predictive mechanical model of leaping was developed using the lesser bushbaby, Galago moholi, as a size-matched leaping prosimian comparison. This enabled the required limb movements for a leaping slender loris to be calculated, and hence the torque and power requirements at each of the hindlimb joints. From this information, the maximum feasible leap was calculated for the slender loris morphotype; and it was found that this alone would prevent the animal from leaping a greater distance than it could walk over, so that the reduction in fitness due to an apparent loss of leaping from the behavioural repertoire can be considered to be very small.

Automatic monitoring of primate locomotor behaviour using accelerometers.

Accelerometry data were transmitted by a radio collar attached to a hand-reared red-ruffed lemur housed in a large indoor/outdoor enclosure at Chester Zoo. An observer simultaneously recorded locomotor behaviour using a manually operated event recorder. Both data streams were recorded directly to hard disk to ensure accurate synchrony. Leaps were modelled using a y = x2 - x3 formulation for the take-off acceleration, to link peak acceleration to leap distance. Cyclic locomotor modes were analysed using power spectra and the modal frequency used to estimate stride periodicity. Comparison of the dual data shows that leaping behaviour can be recorded reliably, and acceleration magnitude provides accurate predictions of the distance travelled. Cyclic activities were less well characterised, but calibration should permit travel distance estimations equalling or bettering those from conventional techniques.

YETI: Yeast Exploration Tool Integrator.

UNLABELLED: Yeast Exploration Tool Integrator (YETI) is a novel bioinformatics tool for the integrated visualization and analysis of functional genomic data sets from the budding yeast Saccharomyces cerevisiae. AVAILABILITY: YETI is freely available for use over the WWW, or download under license, at http://www.bru.ed.ac.uk/~orton/yeti.html

Predicting the metabolic energy costs of bipedalism using evolutionary robotics.

To understand the evolution of bipedalism among the hominoids in an ecological context we need to be able to estimate the energetic cost of locomotion in fossil forms. Ideally such an estimate would be based entirely on morphology since, except for the rare instances where footprints are preserved, this is the only primary source of evidence available. In this paper we use evolutionary robotics techniques (genetic algorithms, pattern generators and mechanical modeling) to produce a biomimetic simulation of bipedalism based on human body dimensions. The mechanical simulation is a seven-segment, two-dimensional model with motive force provided by tension generators representing the major muscle groups acting around the lower-limb joints. Metabolic energy costs are calculated from the muscle model, and bipedal gait is generated using a finite-state pattern generator whose parameters are produced using a genetic algorithm with locomotor economy (maximum distance for a fixed energy cost) as the fitness criterion. The model is validated by comparing the values it generates with those for modern humans. The result (maximum efficiency of 200 J m(-1)) is within 15% of the experimentally derived value, which is very encouraging and suggests that this is a useful analytic technique for investigating the locomotor behaviour of fossil forms. Initial work suggests that in the future this technique could be used to estimate other locomotor parameters such as top speed. In addition, the animations produced by this technique are qualitatively very convincing, which suggests that this may also be a useful technique for visualizing bipedal locomotion.

Early nephron formation in the developing mouse kidney.

This paper reports 3-dimensional confocal microscopy observations on how nephrogenic aggregates form from the NCAM- and Pax2-positive caps (4-5 cells deep) of condensed metanephric mesenchyme surrounding the duct tips of the mouse kidney. Aggregates of 6-8 cells are first seen at approximately E12.5-12.75 immediately proximal to this cap, closely abutting the duct surface. As the tip advances, NCAM expression is maintained in the cap but is otherwise restricted to aggregates whose cells rapidly epithelialise, forming tubules that invade the duct epithelium. Pax2 expression studies shows how the rind of nephrogenic blastemal cells forms: as duct tips extend towards the kidney surface, the associated Pax2+ cells form patches of cells on the kidney surface. These observations revise our knowledge of the timing and process of nephron initiation.