An Analysis of Social and Coping Alcohol Use Motives for College Students with Autistic Traits.

Background: Little is known about alcohol use among college students with autism spectrum disorder (ASD), despite the increasing prevalence of college students with this diagnosis and/or with no formal diagnosis but who would meet criteria. Of concern, previous research suggests that individuals with ASD may be particularly vulnerable to the coping and social facilitation effects of alcohol use. Objectives: The present study examined the associated between autistic traits and alcohol use motives (social, coping, conformity, enhancement) in a sample of college students. Symptoms of social anxiety were assessed as a moderator and thought to enhance the associations between autistic traits and social and coping motives. Results: Findings revealed that both autistic traits and social anxiety were significantly and positively correlated with coping and conformity drinking motives. Additionally, a significantly negative correlation emerged between autistic traits and social drinking motives for participants with low social anxiety, and a similar pattern emerged for enhancement drinking motives. Conclusions: These findings suggest that college students with autistic traits may experience daily encounters or emotions that are alleviated by the mood-altering effects of alcohol; however, the specific feelings, emotions, or experiences that these individuals are looking to seek relief from remain understudied.

Cross-limb communication during Xenopus hindlimb regenerative response: non-local bioelectric injury signals.

Regeneration of damaged body parts requires coordination of size, shape, location and orientation of tissue with the rest of the body. It is not currently known to what extent injury sites communicate with the remaining soma during repair, or what information may emanate from the injury site and reach other regions. We examined the bioelectric properties (resting potential gradients in the epidermis) of Xenopus laevis froglets undergoing hindlimb amputation and observed that the contralateral (undamaged) limb exhibits apparent depolarization signals immediately after the opposite hindlimb is amputated. The pattern of depolarization matches that of the amputated limb and is correlated to the position and type of injury, revealing that information about damage is available to remote body tissues and is detectable non-invasively in vivo by monitoring the bioelectric state. These data extend knowledge about the electrophysiology of regenerative response, identify a novel communication process via long-range spread of injury signaling, a phenomenon that we call bioelectric injury mirroring, and suggest revisions both to regenerative medicine and diagnostic strategies that are focused entirely on the wound site, and to the use of contralateral limbs as controls.

Intersecting epidemics: the impact of coronavirus disease 2019 on the HIV prevention and care continua in the United States.

OBJECTIVE: To summarize the broad impact of the coronavirus disease 2019 (COVID-19) pandemic on HIV prevention and care in the United States with a focus on the status-neutral HIV care continuum. DESIGN: We conducted an editorial review of peer-reviewed literature on the topics of HIV-risk behaviors, sexually transmitted illness (STI) and HIV prevalence, HIV prevention and treatment trends, and evolving practices during the COVID-19 pandemic. METHODS: For relevant literature, we reviewed, summarized, and categorized into themes that span the HIV prevention and care continua, including sexual risk behaviors, mental health, and substance use. RESULTS: We identified important changes within each component of the HIV care continuum across the United States during the COVID-19 pandemic. Shifts in prevention practices, engagement with care, care provision, medication adherence, testing, and prevalence rates were observed during the pandemic. CONCLUSION: Although heightened disparities for people at risk for, and living with, HIV were seen during the COVID-19 pandemic, many health systems and clinics have achieved and maintained engagement in HIV prevention and care. This review highlights barriers and innovative solutions that can support durable and accessible health systems through future public health crises.

Magnetodielectric detection of magnetic quadrupole order in Ba(TiO)Cu4(PO4)4 with Cu4O12 square cupolas.

In vortex-like spin arrangements, multiple spins can combine into emergent multipole moments. Such multipole moments have broken space-inversion and time-reversal symmetries, and can therefore exhibit linear magnetoelectric (ME) activity. Three types of such multipole moments are known: toroidal; monopole; and quadrupole moments. So far, however, the ME activity of these multipole moments has only been established experimentally for the toroidal moment. Here we propose a magnetic square cupola cluster, in which four corner-sharing square-coordinated metal-ligand fragments form a noncoplanar buckled structure, as a promising structural unit that carries an ME-active multipole moment. We substantiate this idea by observing clear magnetodielectric signals associated with an antiferroic ME-active magnetic quadrupole order in the real material Ba(TiO)Cu4(PO4)4. The present result serves as a useful guide for exploring and designing new ME-active materials based on vortex-like spin arrangements.

Stimulatory effect of vanadate on 3',5'-cyclic guanosine monophosphate-inhibited low Michaelis-Menten constant 3',5'-cyclic adenosine monophosphate phosphodiesterase activity in isolated rat fat pads.

When isolated rat fat pads were incubated with vanadate, the low Michaelis-Menten constant (Km) cAMP phosphodiesterase (PDE) activity in the microsomal fraction was increased in a time- and dose-dependent manner with vanadate. 3',5'-Cyclic GMP inhibited the vanadate-stimulated PDE activity with similar profile to the insulin-stimulated one. The stimulatory effect of vanadate was inhibited by inhibitors of tyrosine kinases such as amiloride, biochanin A, and genistein to various extents. Vanadate and insulin both showed the full effect in the absence of either K+, N+, or Ca2+ in the medium, while preincubation of the fat pads with a chelator of intracellular Ca2+ inhibited the vanadate action in a dose-dependent manner. The insulin action was not inhibited by it at tested concentrations. These results suggest that the vanadate action, in contrast to the insulin one, is dependent on the intracellular level of Ca2+. Preincubation of the fat pads with inhibitors of protein kinase C such as 1-(5-isoquinoline sulfonyl)-2-methylpiperazine (H-7) and staurosporine inhibited, in part, the vanadate action but did not inhibit the insulin one. Furthermore, vanadate increased the protein kinase C activity in fat pads but insulin did not increase. H-7 and amiloride showed a significant inhibition of stimulation of protein kinase C activity by vanadate. These results suggest that vanadate stimulates, in part, the 3',5'-cyclic GMP-inhibited low Km cAMP PDE activity in the microsomal fraction of fat pads through the activation of tyrosine kinase and protein kinase C-mediated processes.

The central projection of masticatory afferent fibers to the trigeminal sensory nuclear complex and upper cervical spinal cord.

Retrograde and anterograde transport of horseradish peroxidase-wheat germ agglutinin (HRP-WGA) conjugate was used to study the organization of primary afferent neurons innervating the masticatory muscles. HRP applied to the nerves of jaw-closing muscles--the deep temporal (DT), masseter (Ma), and medial pterygoid (MP)--labeled cells in the trigeminal ganglion and the mesencephalic trigeminal nucleus (Vmes), whereas HRP applied to nerves of the jaw-opening muscles--anterior digastric (AD) and mylohyoid (My)--labeled cells only in the trigeminal ganglion. Cell bodies innervating the jaw-closing muscles were found with greater frequency in the intermediate region of the mandibular subdivision, while somata supplying the jaw-opening muscles were predominant posterolaterally. The distribution of their somatic sizes was unimodal and limited to a subpopulation of smaller cells. Projections of the muscle afferents of ganglionic origin to the trigeminal sensory nuclear complex (TSNC) were confined primarily to the caudal half of pars interpolaris (Vi), and the medullary and upper cervical dorsal horns. In the Vi, Ma, MP, AD, and My nerves terminated in the lateral-most part of the nucleus with an extensive overlap in projections, save for the DT nerve, which projected to the interstitial nucleus or paratrigeminal nucleus. In the medullary and upper cervical dorsal horns, the main terminal fields of individual branches were confined to laminae I/V, but the density of the terminals in lamina V was very sparse. The rostrocaudal extent of the terminal field in lamina I differed among the muscle afferents of origin, whereas in the mediolateral or dorsoventral axis, a remarkable overlap in projections was noted between or among muscle afferents. The terminals of DT afferents were most broadly extended from the rostral level of the pars caudalis to the C3 segment, whereas the MP nerve showed limited projection to the middle one-third of the pars caudalis. Terminal fields of the Ma, AD, and My nerves appeared in the caudal two-thirds of the pars caudalis including the first two cervical segments, the caudal half of the pars caudalis and the C1 segment, and in the caudal part of the pars caudalis including the rostral C1 segment, respectively. This rostrocaudal arrangement in the projections of muscle nerves, which corresponds to the anteroposterior length of the muscles and their positions, indicates that representation of the masticatory muscles in lamina I reflects an onion-skin organization. These results suggest that primary muscle afferent neurons of ganglionic origin primarily mediate muscle pain.(ABSTRACT TRUNCATED AT 400 WORDS)

Oral and facial representation within the medullary and upper cervical dorsal horns in the cat.

Transganglionic transport of HRP was used to study the patterns of termination of somatic afferent fibers innervating oral and facial structures within the trigeminal nucleus caudalis and upper cervical dorsal horn of the cat. In separate animals, the superior alveolar, pterygopalatine, buccal, inferior alveolar, lingual, frontal, corneal, zygomatic, infraorbital, mental, mylohyoid, and auriculotemporal branches of the trigeminal nerve were traced in this experiment. The organization of the primary afferents innervating the oral structures is not uniform across laminae and at different rostrocaudal levels of the nucleus caudalis. The superior alveolar and pterygopalatine nerves mainly terminate in laminae I, II, and V at the level of the rostral one-third of the caudalis. By contrast, the lingual, inferior alveolar, and buccal nerve terminate in laminae I-V of, respectively, the rostral third, the entire length, and caudal two-thirds of the caudalis. In addition, the lingual, buccal, and pterygopalatine nerves terminate in the dorsal and middle parts of the interstitial islands or pockets of lamina I neuropil extending to the rostral levels parallel to the nucleus interpolaris. Mediolaterally, in laminae I, II, and V of the rostral third an extensive overlap of projections was found between the branches from each trigeminal division, and some overlap was observed between projections from the mandibular and maxillary divisions. On the other hand, the projections of primary afferents innervating the facial structures are arranged in a somatotopic fashion in rostrocaudal and mediolateral axes over the laminae (I-IV) through the nucleus caudalis and upper cervical dorsal horn. Fibers from the perioral and perinasal regions terminate most rostrally in caudalis, and fibers from progressively more posterior facial regions terminate at successively lower levels. A mediolateral somatotopic arrangement was observed, with fibers from the ventral parts of face ending in the medial regions and fibers from the progressively more dorsal parts of the face ending in successively more lateral regions of the medullary and upper cervical dorsal horns. Corneal afferent terminals are concentrated in the outer parts of lamina II at the levels of the rostral parts of the caudal two-thirds of the caudalis and the interstitial islands of lamina I. The maxillary division terminates first at the most caudal level of the caudalis, followed by the ophthalmic division descending as far as the C2 segment and the mandibular division reaching the most caudal level of the C2 segment.(ABSTRACT TRUNCATED AT 400 WORDS)

Representation of upper and lower primary teeth in the trigeminal sensory nuclear complex in the young dog.

Transganglionic transport of horseradish peroxidase-wheat germ agglutinin conjugate entrapped in polyacrylamide gel was used to study the patterns of termination of primary afferents that innervate the upper and lower primary tooth pulps within the trigeminal sensory nuclear complex of the young dog. The lower and upper primary tooth pulp afferents projected to the subnucleus dorsalis of the principal nucleus, the rostrodorsomedial part and subnucleus dorsomedialis (Vo.dm) of the pars oralis, the nucleus of the intermediate plexus (Vi.ip) of the pars interpolaris, and laminae I, II and V of the caudalis. The lower and upper primary teeth were topographically represented in the Vo.dm, rostrocaudal mid-levels of Vi.ip, and in laminae I/V of the caudal levels of the pars caudalis, whereas an extensive overlapped projection was seen in other subdivisions.

The distribution of muscle primary afferents from the masseter nerve to the trigeminal sensory nuclei.

Transganglionic transport of horseradish peroxidase--wheat germ agglutinin conjugate was used to study the pattern of termination of somatic afferent fibers innervating the masseter muscle within the trigeminal sensory nuclear complex (TSNC) of the cat. The central processes of the masseteric nerve terminated in the caudal third of the pars interpolaris, and laminae I/V through the caudal two-thirds of caudalis and rostral parts of the C1 spinal cord segment. The functional significance of the masseteric afferent projections to the TSNC with a preferential pattern was discussed, particularly with respect to muscle pain.

Laminar-related projection of primary trigeminal fibers in the caudal medulla demonstrated by transganglionic transport of horseradish peroxidase.

The mode of termination of primary afferent fibers within the cat trigeminal nucleus caudalis was investigated by means of the transganglionic transport of horseradish peroxidase (HRP). Several types of laminar-related labeling were observed, depending upon the survival time after HRP application. At the earliest survival time (28-34 h) the highest density of labeling was found in laminae I and II. At 2 and 3 days survival laminae III and IV were heavily labeled, in addition to laminae I and II where the amount of labeling was greatly increased in lamina I, but not in lamina II. At 5 days survival time an abrupt drop of labeling occurred in laminae I and II, while this pattern was not predominant in laminae III and IV. In lamina V the pattern of labeling was less intense and not changeable through all survival times observed. These findings indicating a differentiation of the primary afferent terminals have good correspondence with a functional specialization of neuronal locations since the functional properties of neurons vary according to their locations.

Characteristics of a cheese-like food produced by fermentation of the mushroom Schizophyllum commune.

Lactate bacteria of the Lactobacillus and Streptococcus genera are normally employed in cheese making because these microbes have potent ability to produce lactate dehydrogenase. A milk-clotting enzyme is also necessary to make cheese. Recently, we discovered that some mushroom genera produce both lactate dehydrogenase and a milk-clotting enzyme. Using the mushroom Schizophyllum commune in place of a lactate bacterium, we produced a cheese-like food that contained about 0.58% beta-D-glucan, which has been shown to have preventive effects against cancer. The food also exhibited thrombosis prevention activity, prolonging the thrombin clotting time to 49.6-fold that of the control.

Effects of rapid burnout type gypsum-bonded investment on performance of castings. Part 1. Surface aspects and fit of crowns.

Rapid burnout type gypsum-bonded investment materials were developed to shorten the time required for dental casting procedures. With these materials, molds can be prepared by rapid heating at 700 degrees C for 30 min from 30 min after the start of mixing. When the investment block was rapidly heated at 700 degrees C, no fractures were observed in the rapid burnout type investments with one exception, while a conventional cristobalite investment broke into pieces shortly after being placed in the furnace. Casting fins were sometimes induced only for the material which showed fracturing on rapid heating. No practical problems were found in the surface roughness of the castings. The 30 min-setting expansion was significantly different among the materials although there were no differences in thermal expansion, and the material showing greater 30 min-setting expansion was efficient to obtain better fit of the crown as in the conventional casting procedures.