RESUMEN
The biotic-abiotic photosynthetic system integrating inorganic light absorbers with whole-cell biocatalysts innovates the way for sustainable solar-driven chemical transformation. Fundamentally, the electron transfer at the biotic-abiotic interface, which may induce biological response to photoexcited electron stimuli, plays an essential role in solar energy conversion. Herein, we selected an electro-active bacterium Shewanella oneidensis MR-1 as a model, which constitutes a hybrid photosynthetic system with a self-assembled CdS semiconductor, to demonstrate unique biotic-abiotic interfacial behavior. The photoexcited electrons from CdS nanoparticles can reverse the extracellular electron transfer (EET) chain within S. oneidensis MR-1, realizing the activation of a bacterial catalytic network with light illumination. As compared with bare S. oneidensis MR-1, a significant upregulation of hydrogen yield (711-fold), ATP, and reducing equivalent (NADH/NAD+) was achieved in the S. oneidensis MR-1-CdS under visible light. This work sheds light on the fundamental mechanism and provides design guidelines for biotic-abiotic photosynthetic systems.
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Electrones , Shewanella , Transporte de Electrón , Hidrógeno , FotosíntesisRESUMEN
The biotransformation of heavy metals in the environment is usually affected by co-existing pollutants like selenium (Se), which may lower the ecotoxicity of heavy metals, but the underlying mechanisms remain unclear. Here, we shed light on the pathways of copper (Cu2+) and selenite (SeO32-) synergistic biodetoxification by Shewanella oneidensis MR-1 and illustrate how such processes are affected by anthraquinone-2,6-disulfonate (AQDS), an analogue of humic substances. We observed the formation of copper selenide nanoparticles (Cu2-xSe) from synergistic detoxification of Cu2+ and SeO32- in the periplasm. Interestingly, adding AQDS triggered a fundamental transition from periplasmic to extracellular reaction, enabling 14.7-fold faster Cu2+ biodetoxification (via mediated electron transfer) and 11.4-fold faster SeO32- detoxification (via direct electron transfer). This is mainly attributed to the slightly raised redox potential of the heme center of AQDS-coordinated outer-membrane proteins that accelerates electron efflux from the cells. Our work offers a fundamental understanding of the synergistic detoxification of heavy metals and Se in a complicated environmental matrix and unveils an unexpected role of AQDS beyond electron mediation, which may guide the development of more efficient environmental remediation and resource recovery biotechnologies.
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Contaminantes Ambientales , Selenio , Antraquinonas , Cobre , Hemo , Sustancias Húmicas , Proteínas de la Membrana , Oxidación-Reducción , Ácido SeleniosoRESUMEN
Mercury (Hg), as a highly harmful environmental pollutant, poses severe ecological and health risks even at low concentrations. Accurate and sensitive methods for detecting Hg2+ ions in aquatic environments are highly needed. In this work, we developed a highly sensitive fluorescence sensor for Hg2+ detection with an integrated use of biosynthetic CdSe/CdS quantum dots (QDs) and liposome carrier signal amplification. To construct such a sensor, three single-stranded DNA probes were rationally designed based on the thymine-Hg2+-thymine (T-Hg2+-T) coordination chemical principles and by taking advantage of the biocompatibility and facile-modification properties of the biosynthetic QDs. Hg2+ could be determined in a range from 0.25 to 100 nM with a detection limit of 0.01 nM, which met the requirements of environmental sample detection. The sensor also exhibited a high selectivity for Hg2+ detection in the presence of other high-level metal ions. A satisfactory capacity of the sensor for detecting environmental samples including tap water, river water, and landfill leachate was also demonstrated. This work opens up a new application scenario for biosynthetic QDs and holds a great potential for environmental monitoring applications.
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Liposomas/química , Mercurio/análisis , Puntos Cuánticos/química , Espectrometría de Fluorescencia/métodos , Compuestos de Cadmio/química , ADN de Cadena Simple/química , Monitoreo del Ambiente , Agua Dulce/análisis , Concentración de Iones de Hidrógeno , Límite de Detección , Compuestos de Selenio/química , Sulfuros/química , Timina/química , Contaminantes Químicos del Agua/análisisRESUMEN
Biotransformation of selenite to valuable elemental selenium nanoparticles (Se0) is a promising avenue to remediate seleniferous environments and simultaneously recover selenium (Se). However, the underlying oxyanion competition and selenite transformation mechanism in prokaryotes are poorly understood. In this work, the impacts of phosphate on selenite uptake and transformation were elucidated with Escherichia coli and its mutant deficient in phosphate transport as model microbial strains. Selenite uptake was inhibited by phosphate in E. coli. Moreover, the transformation of internalized Se was shifted from Se0 to toxic organo-Se with elevated phosphate levels, as evidenced by the linear combination fit analysis of the Se K-edge X-ray absorption near-edge structure. Such a phosphate-regulated selenite biotransformation process was mainly assigned to the competitive uptake of phosphate and selenite, which was primarily mediated by a low affinity phosphate transporter (PitA). Under phosphate-deficient conditions, the cells not only produced abundant Se0 nanoparticles but also maintained good cell viability. These findings provide new insights into the phosphate-regulated selenite biotransformation by prokaryotes and contribute to the development of new processes for bioremediating Se-contaminated environments, as well as bioassembly of Se0.
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Ácido Selenioso , Selenio , Biotransformación , Escherichia coli , Fosfatos , Selenito de SodioRESUMEN
Antagonism between heavy metal and selenium (Se) could significantly affect their biotoxicity, but little is known about the mechanisms underlying such microbial-mediated antagonistic processes as well as the formed products. In this work, we examined the cadmium (Cd)-Se interactions and their fates in Caenorhabditis elegans through in vivo and in vitro analysis and elucidated the machinery of Se-stimulated Cd detoxification. Although the Se introduction induced up to 3-fold higher bioaccumulation of Cd in C. elegans than the Cd-only group, the nematode viability remained at a similar level to the Cd-only group. The relatively lower level of reactive oxygen species in the Se & Cd group confirms a significantly enhanced Cd detoxification by Se. The Cd-Se interaction, mediated by multiple thiols, including glutathione and phytochelatin, resulted in the formation of less toxic cadmium selenide (CdSe)/cadmium sulfide (CdS) nanoparticles. The CdSe/CdS nanoparticles were mainly distributed in the pharynx and intestine of the nematodes, and continuously excreted from the body, which also benefitted the C. elegans survival. Our findings shed new light on the microbial-mediated Cd-Se interactions and may facilitate an improved understanding and control of Cd biotoxicity in complicated coexposure environments.
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Nanopartículas , Selenio , Animales , Cadmio , Caenorhabditis elegans , Compuestos de SulfhidriloRESUMEN
Quantum dots (QDs) are recognized as the excellent fluorescence and photochemical materials to be applied in bioimaging, biomedical, and solar cell fields. Biosynthesized QDs (bio-QDs) have attracted attention due to their simple, eco-friendly, and excellent biocompatible traits. Moreover, bio-QDs could not be replaced by chemically fabricated QDs in many fields. Bio-QDs synthesized by different microorganisms have diverse characteristics. In this work, the biosynthesis of QDs by Tetrahymena pyriformis, a typical protozoa in aquatic environments, was achieved for the first time. The synthesized materials by T. pyriformis emitted yellow fluorescence and had an average diameter of 8.27 ± 0.77 nm. Spectral characterization results demonstrated that the synthesized QDs were CdS1-XSeX. Meanwhile, the fluorescence intensities of the synthesized bio-QDs showed a linear relationship with Cd2+ dosage ranging from 20 to 80 µM. The fluorescence enhancement of the synthesized QDs was highly selective to Cd2+ compared to other metal ions. The bio-QDs were demonstrated to have a great potential to be applied for Cd2+ detection. This work provides valuable information about the transformation of heavy metal ions in protozoan and is useful to accelerate the applications of the synthesized QDs.
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Cadmio/análisis , Ingeniería Metabólica/métodos , Redes y Vías Metabólicas/genética , Puntos Cuánticos/metabolismo , Tetrahymena pyriformis/metabolismo , Cationes Bivalentes/análisis , Fluorescencia , Tecnología Química Verde/métodos , Metales Pesados/análisis , Puntos Cuánticos/química , Análisis Espectral , Tetrahymena pyriformis/genéticaRESUMEN
Biofabrication of nanomaterials is currently constrained by a low production efficiency and poor controllability on product quality compared to chemical synthetic routes. In this work, we show an attractive new biosynthesis system to break these limitations. A directed production of selenium-containing nanoparticles in Shewanella oneidensis MR-1 cells, with fine-tuned composition and subcellular synthetic location, was achieved by modifying the extracellular electron transfer chain. By taking advantage of its untapped intracellular detoxification and synthetic power, we obtained high-purity, uniform-sized cadmium selenide nanoparticles in the cytoplasm, with the production rates and fluorescent intensities far exceeding the state-of-the-art biosystems. These findings may fundamentally change our perception of nanomaterial biosynthesis process and lead to the development of fine-controllable nanoparticles biosynthesis technologies.
RESUMEN
Biotic-abiotic hybrid photocatalytic system is an innovative strategy to capture solar energy. Diversifying solar energy conversion products and balancing photoelectron generation and transduction are critical to unravel the potential of hybrid photocatalysis. Here, we harvest solar energy in a dual mode for Cu2-xSe nanoparticles biomineralization and seawater desalination by integrating the merits of Shewanella oneidensis MR-1 and biogenic nanoparticles. Photoelectrons generated by extracellular Se0 nanoparticles power Cu2-xSe synthesis through two pathways that either cross the outer membrane to activate periplasmic Cu(II) reduction or are directly delivered into the extracellular space for Cu(I) evolution. Meanwhile, photoelectrons drive periplasmic Cu(II) reduction by reversing MtrABC complexes in S. oneidensis. Moreover, the unique photothermal feature of the as-prepared Cu2-xSe nanoparticles, the natural hydrophilicity, and the linking properties of bacterium offer a convenient way to tailor photothermal membranes for solar water production. This study provides a paradigm for balancing the source and sink of photoelectrons and diversifying solar energy conversion products in biotic-abiotic hybrid platforms.
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Biomineralización , Cobre , Agua de Mar , Shewanella , Energía Solar , Shewanella/metabolismo , Cobre/química , Cobre/metabolismo , Agua de Mar/microbiología , Agua de Mar/química , Salinidad , Purificación del Agua/métodos , Nanopartículas/química , Catálisis/efectos de la radiaciónRESUMEN
Understanding the spatial orientation of nanoparticles and the corresponding subcellular architecture events favors uncovering fundamental toxic mechanisms and predicting response pathways of organisms toward environmental stressors. Herein, we map the spatial location of label-free citrate-coated Ag nanoparticles (Cit-AgNPs) and the corresponding subcellular reorganization in microalgae by a noninvasive 3D imaging approach, cryo-soft X-ray tomography (cryo-SXT). Cryo-SXT near-natively displays the 3D maps of Cit-AgNPs presenting in rarely identified sites, namely, extracellular polymeric substances (EPS) and the cytoplasm. By comparative 3D morphological assay, we observe that Cit-AgNPs disrupt the cellular ultrastructural homeostasis, triggering a severe malformation of cytoplasmic organelles with energy-producing and stress-regulating functions. AgNPs exposure causes evident disruption of the chloroplast membrane, significant attenuation of the pyrenoid matrix and starch sheath, extreme swelling of starch granules and lipid droplets, and shrinkage of the nucleolus. In accompaniment, the number and volume occupancy of starch granules are significantly increased. Meanwhile, the spatial topology of starch granules extends from the chloroplast to the cytoplasm with a dispersed distribution. Linking the dynamics of the internal structure and the alteration of physiological properties, we derive a comprehensive cytotoxic and response pathway of microalgae exposed to AgNPs. This work provides a perspective for assessing the toxicity at subcellular scales to achieve label-free nanoparticle-caused ultrastructure remodeling of phytoplankton.
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Nanopartículas del Metal , Microalgas , Nanopartículas del Metal/química , Plata/química , Citoplasma/metabolismo , AlmidónRESUMEN
Analysis of cellular ultrastructure dynamics and metal ions' fate can provide insights into the interaction between living organisms and metal ions. Here, we directly visualize the distribution of biogenic metallic aggregates, ion-induced subcellular reorganization, and the corresponding regulation effect in yeast by the near-native 3D imaging approach, cryo-soft X-ray tomography (cryo-SXT). By comparative 3D morphometric assessment, we observe the gold ions disrupting cellular organelle homeostasis, resulting in noticeable distortion and folding of vacuoles, apparent fragmentation of mitochondria, extreme swelling of lipid droplets, and formation of vesicles. The reconstructed 3D architecture of treated yeast demonstrates â¼65% of Au-rich sites in the periplasm, a comprehensive quantitative assessment unobtained by TEM. We also observe some AuNPs in rarely identified subcellular sites, namely, mitochondria and vesicles. Interestingly, the amount of gold deposition is positively correlated with the volume of lipid droplets. Shifting the external starting pH to near-neutral results in the reversion of changes in organelle architectures, boosting the amount of biogenic Au nanoparticles, and increasing cell viability. This study provides a strategy to analyze the metal ions-living organism interaction from subcellular architecture and spatial localization perspectives.
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Oro , Nanopartículas del Metal , Oro/química , Saccharomyces cerevisiae , Orgánulos/metabolismo , MitocondriasRESUMEN
Cadmium (Cd) is a typical and widely present toxic heavy metals in environments. Biomineralization of Cd ions could alleviate the toxicity and produce valuable products in certain waste streams containing selenite. However, the impact of the intrinsic Cd(II) efflux system on the biotransformation process remains unrevealed. In this work, the significance of the efflux system on Cd biomineralization was evaluated by constructing engineered Escherichia coli strains, including ΔzntA with suppressed Cd(II) efflux system and pYYDT-zntA with strengthened Cd(II) efflux system. Compared to the wild type (WT), 20% more Cd ions were accumulated in ΔzntA and 17% less were observed in pYYDT-zntA in the presence of selenite as determined by inductively coupled plasma atomic emission spectrometer. Through combination with X-ray absorption fine structure analysis, it was discovered that 50% higher production of CdSxSe1-x quantum dots (QDs) was achieved in the ΔzntA cells than that in the WT cells. Moreover, the ΔzntA cells exhibited the same viability as the WT cells and the pYYDT-zntA cells because accumulated Cd ions were transformed into biocompatible QDs. In addition, the biosynthesized QDs had a uniform particle size (3.82 ± 0.53 nm) and a long fluorescence lifetime (45.6 ns), which could potentially be utilized for bio-imaging. These results not only elucidate the significance of Cd(II) efflux system in the biotransformation of Cd ions and selenite, but also provide a promising way to recover Cd and Se as valuable products in certain waste streams.
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Proteínas de Transporte de Catión , Puntos Cuánticos , Biomineralización , Cadmio/metabolismo , Cadmio/toxicidad , Cationes , Escherichia coli/genética , Escherichia coli/metabolismoRESUMEN
Biosynthesis has gained growing interest due to its energy efficiency and environmentally benign nature. Recently, biogenic iron sulfide nanoparticles (FeS NPs) have exhibited excellent performance in environmental remediation and energy recovery applications. However, their biosynthesis regulation strategy and application prospects in the biomedical field remain to be explored. Herein, biogenic FeS NPs are controllably synthesized by Shewanella oneidensis MR-1 and applied for cancer therapy. Tuning the synthesis rate and yield of biogenic FeS NPs is realized by altering the initial iron precursor dosage. Notably, increasing the precursor concentration decreases and delays FeS NP biosynthesis. The biogenic FeS NPs (30 nm) are homogeneously anchored on the cell surface of S. oneidensis MR-1. Moreover, the good hydrophilic nature and outstanding Fenton properties of the as-prepared FeS NPs endow them with good cancer therapy performance. The intracellular location of the FeS NPs taken up is visualized with a soft X-ray microscope (SXM). Highly efficient cancer cell killing can be achieved at extremely low concentrations (<12 µg mL-1), lower than those in reported works. Such good performance is attributed to the Fe2+ release, elevated ROS, reduced glutathione (GSH) consumption, and lipid hydroperoxide (LPO) generation. The resulting FeS NPs show excellent in vivo therapeutic performance. This work provides a facile, eco-friendly, and scalable approach to produce nanomedicine, demonstrating the potential of biogenic nanoparticles for use in cancer therapy.
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Restauración y Remediación Ambiental , Nanopartículas , Neoplasias , Shewanella , Hierro , Neoplasias/tratamiento farmacológicoRESUMEN
Incorporating artificial photosensitizers with microorganisms has recently been recognized as an effective way to convert light energy into chemical energy. However, the incorporated biosystem is usually constructed in an extracellular manner and is vulnerable to the external environment. Here, we develop an intracellular hybrid biosystem in a higher organism protozoa Tetrahymena pyriformis, in which the in vivo synthesized CdS nanoparticles trigger photoreduction of nitrobenzene into aniline under visible-light irradiation. Integrating a photosensitizer CdS into T. pyriformis enables the photosensitizer CdS, inherent nitroreductase, and the cytoplasmic reductive substance in T. pyriformis to synergistically engage in the photocatalysis process, generating a greatly enhanced aniline yield with a 40-fold increment. Moreover, building an intracellular hybrid biosystem in mutant T. pyriformis could even grant it new capability of reducing nitrobenzene into aniline under visible-light irradiation. Such an intracellular hybrid biosystem paves a new way to functionalize higher organisms and diversify light energy conversion.
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Luz , Tetrahymena pyriformis/metabolismo , Compuestos de Anilina/metabolismo , Compuestos de Cadmio/química , Compuestos de Cadmio/farmacología , Catálisis , Nanopartículas del Metal/química , Microscopía Fluorescente/métodos , Mutación , Nitrobencenos/metabolismo , Fármacos Fotosensibilizantes/química , Fármacos Fotosensibilizantes/farmacología , Sulfuros/química , Sulfuros/farmacología , Tetrahymena pyriformis/genéticaRESUMEN
Nanoparticle-based fluorescent probes, typically fabricated by a chemical synthesis route, have been widely used for monitoring trace heavy metals in environments. However, the high-cost and complicated, aggressive fabrication processes restrict their widespread application. In this work, we report the first use of biogenic quantum dots (Bio-QDs) as a highly sensitive, low-cost fluorescent probe for label-free detection of mercury ions (Hg2+), with comparable performance to conventional chemically synthesized counterparts. Fluorescent Bio-QDs with uniform sizes (1.6 ± 0.3 nm) and unique core-shell structure (CdSxSe1-x core and protein- and phosphate-rich capping) were assembled by Escherichia coli cells. The Bio-QDs were extracted and directly used as a Hg2+ probe, which exhibited sensitive, linear fluorescent response to Hg2+ concentration in the range of 1.5-100 nM. Interestingly, it even enable a naked-eye detection of Hg2+ in a higher concentration range of 0.1-10 µM by simply raising the Bio-QD load. The underlying detection mechanisms, involving substitution of the Cd atoms with Hg from water, were revealed by Raman spectra, X-ray absorption fine structure, and density functional theory calculations. Our work implies a high potential of green-synthesized Bio-QDs for environmental monitoring applications, which may not only broaden the application ranges of Bio-QDs, but also advance the development of environmental analytical techniques toward higher sustainability.
RESUMEN
Biosynthesis offers opportunities for cost-effective and sustainable production of semiconductor quantum dots (QDs), but is currently restricted by poor controllability on the synthesis process, resulting from limited knowledge on the assembly mechanisms and the lack of effective control strategies. In this work, we provide molecular-level insights into the formation mechanism of biogenic QDs (Bio-QDs) and its connection with the cellular substrate metabolism in Escherichia coli. Strengthening the substrate metabolism for producing more reducing power was found to stimulate the production of several reduced thiol-containing proteins (including glutaredoxin and thioredoxin) that play key roles in Bio-QDs assembly. This effectively diverted the transformation route of the selenium (Se) and cadmium (Cd) metabolic from Cd3(PO4)2 formation to CdS xSe1- x QDs assembly, yielding fine-sized (2.0 ± 0.4 nm), high-quality Bio-QDs with quantum yield (5.2%) and fluorescence lifetime (99.19 ns) far exceeding the existing counterparts. The underlying mechanisms of Bio-QDs crystallization and development were elucidated by density functional theory calculations and molecular dynamics simulation. The resulting Bio-QDs were successfully used for bioimaging of cancer cells and tumor tissue of mice without extra modification. Our work provides fundamental knowledge on the Bio-QDs assembly mechanisms and proposes an effective, facile regulation strategy, which may inspire advances in controlled synthesis and practical applications of Bio-QDs as well as other bionanomaterials.
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Cadmio/química , Imagen Molecular/métodos , Puntos Cuánticos/química , Selenio/química , Animales , Cadmio/farmacología , Supervivencia Celular/efectos de los fármacos , Escherichia coli/efectos de los fármacos , Fluorescencia , Glutarredoxinas/química , Glutarredoxinas/genética , Humanos , Ratones , Microscopía Fluorescente/métodos , Puntos Cuánticos/metabolismo , Selenio/farmacología , Especificidad por Sustrato/efectos de los fármacos , Tiorredoxinas/química , Tiorredoxinas/genéticaRESUMEN
Ciprofloxacin (CIP), as an extensively used antibiotic, has been widely detected at a high level in the environment and has raised environmental pollution concerns. Thus, efficient and cost-effective methods for CIP degradation are highly desired. Biologically produced manganese oxides (BioMnOx) offer a promising perspective for CIP degradation because of their catalytic reactivity and cost-effectiveness. However, the release of Mn(II) from BioMnOx prevents the further oxidation of pollutants. As a consequence, continuous CIP degradation by BioMnOx is not feasible. In this work, a manganese redox cycling system driven by Pseudomonas putida MnB-1 was constructed for continuous degradation of CIP. In such a system CIP was oxidized continuously and rapidly by re-oxidizing the formed Mn(II) to regenerate reactive BioMnOx, which also protected the strain from CIP toxicity. CIP was degraded through N-dealkylation passway. No significant loss of BioMnOx reactivity was observed in three-cycle CIP degradation process, suggesting the stability of this system. An overlooked intracellular BioMnOx, which was involved in CIP degradation, was discovered in P. putida MnB-1. Moreover, the important role of Mn(III) in facilitating CIP removal in this system was also identified. This work provides useful information to better understand the degradation of antibiotic compounds mediated by microbes in environments.
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Antibacterianos/metabolismo , Ciprofloxacina/metabolismo , Manganeso/química , Manganeso/metabolismo , Pseudomonas putida/metabolismo , Oxidación-ReducciónRESUMEN
The discharge of engineered nanomaterials (ENMs) into environment is raising widespread concern not only due to their direction bio-toxicity but also their bio-concentration and bio-magnification through food web. However, the transformation and distribution of ENMs during food-chain transport are poorly understood, due to lack of accurate, reliable analytical methods. In this study, by using a suite of advanced spectrum techniques, we successfully tracked the distribution and biotransformation dynamics of CdSe quantum dots (QDs) during their transport from Shewanella onedensis to Caenorchabditis elegans in predation. Fluorescence microscopy and Raman mapping showed that the ingested QDs by C. elegans were located at the gut lumen and subcutaneous tissue, and were partially excreted from the nematode body over time. Micro-X-ray fluorescence (µ-XRF) spectroscopy and Se K-edge X-ray absorption fine structure (XAFS) results further revealed the changed distribution of Se element over time, and a shift in the major Se species from CdSe to Se0 and Na2SeO3IV. This work demonstrates the utility of advanced spectral techniques for characterizing QDs in vivo, and may facilitate a better understanding on the environmental transformation and fates of ENMs.
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Biotransformación , Compuestos de Cadmio , Cadena Alimentaria , Puntos Cuánticos , Compuestos de Selenio , Animales , Bacterias/metabolismo , Biodegradación Ambiental , Caenorhabditis elegans/metabolismo , Análisis EspectralRESUMEN
Organisms served as factories of bio-assembly of nanoparticles attracted a lot of attentions due to the safe, economic and environmental-benignity traits, especially the fabrication of the super fluorescence properties quantum dots (QDs). However, information about the developmental dynamics of QDs in living organisms is still lacking. In this work, we synthesized cadmium-selenium (CdSe) QDs in Candida utilis WSH02-08, and then tracked and quantitatively characterized the developmental dynamics (photoactivation, photostable and photobleaching processes) of bio-QDs by translating fluorescence microscopy movies into visual quantitative curve. These findings shed light on the fluorescence properties of the bio-assembled QDs and are expected to accelerate the applications of the synthesized QDs in vivo. It provided a new way to screen bio-QDs and monitor the quality of QDs in vivo.
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Compuestos de Cadmio/metabolismo , Candida/metabolismo , Puntos Cuánticos , Compuestos de Selenio/metabolismo , Compuestos de Cadmio/química , Fluorescencia , Microscopía Fluorescente , Puntos Cuánticos/química , Puntos Cuánticos/ultraestructura , Compuestos de Selenio/química , Espectrometría de FluorescenciaRESUMEN
Photothermal therapy (PTT) is a minimally invasive and effective cancer treatment method and has a great potential for innovating the conventional chemotherapy approaches. Copper sulfide (CuS) exhibits photostability, low cost, and high absorption in near infrared region, and is recognized as an ideal candidate for PTT. However, CuS, as a photothermal agent, is usually synthesized with traditional chemical approaches, which require high temperature, additional stabilization and hydrophilic modification. Herein, we report, for the first time, the preparation of CuS nanoparticles as a photothermal agent by a dissimilatory metal reducing bacterium Shewanella. oneidensis MR-1. The prepared nanoparticles are homogenously shaped, hydrophilic, small-sized (â¼5nm) and highly stable. Furthermore, the biosynthesized CuS nanoparticles display a high photothermal conversion efficiency of 27.2% because of their strong absorption at 1100nm. The CuS nanoparticles could be effectively used as a PTT agent under the irradiation of 1064nm. This work provides a simple, eco-friendly and cost-effective approach for fabricating PTT agents.
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Cobre/química , Cobre/metabolismo , Nanopartículas del Metal/química , Shewanella/metabolismo , Sulfuros/química , Sulfuros/metabolismo , Línea Celular Tumoral , Cobre/farmacología , Tecnología Química Verde , Humanos , Hipertermia Inducida , Nanopartículas del Metal/uso terapéutico , Nanopartículas del Metal/ultraestructura , Procesos Fotoquímicos , Sulfuros/farmacologíaRESUMEN
Although oxygen has been reported to regulate biofilm formation by several Shewanella species, the exact regulatory mechanism mostly remains unclear. Here, we identify a direct oxygen-sensing diguanylate cyclase (DosD) and reveal its regulatory role in biofilm formation by Shewanella putrefaciens CN32 under aerobic conditions. In vitro and in vivo analyses revealed that the activity of DosD culminates to synthesis of cyclic diguanylate (c-di-GMP) in the presence of oxygen. DosD regulates the transcription of bpfA operon which encodes seven proteins including a large repetitive adhesin BpfA and its cognate type I secretion system (TISS). Regulation of DosD in aerobic biofilms is heavily dependent on an adhesin BpfA and the TISS. This study offers an insight into the molecular mechanism of oxygen-stimulated biofilm formation by S. putrefaciens CN32.