Propolis: Its Role and Efficacy in Human Health and Diseases.

With technological advancements in the medicinal and pharmaceutical industries, numerous research studies have focused on the propolis produced by stingless bees (Meliponini tribe) and Apis mellifera honeybees as alternative complementary medicines for the potential treatment of various acute and chronic diseases. Propolis can be found in tropical and subtropical forests throughout the world. The composition of phytochemical constituents in propolis varies depending on the bee species, geographical location, botanical source, and environmental conditions. Typically, propolis contains lipid, beeswax, essential oils, pollen, and organic components. The latter include flavonoids, phenolic compounds, polyphenols, terpenes, terpenoids, coumarins, steroids, amino acids, and aromatic acids. The biologically active constituents of propolis, which include countless organic compounds such as artepillin C, caffeic acid, caffeic acid phenethyl ester, apigenin, chrysin, galangin, kaempferol, luteolin, genistein, naringin, pinocembrin, coumaric acid, and quercetin, have a broad spectrum of biological and therapeutic properties such as antidiabetic, anti-inflammatory, antioxidant, anticancer, rheumatoid arthritis, chronic obstruct pulmonary disorders, cardiovascular diseases, respiratory tract-related diseases, gastrointestinal disorders, as well as neuroprotective, immunomodulatory, and immuno-inflammatory agents. Therefore, this review aims to provide a summary of recent studies on the role of propolis, its constituents, its biologically active compounds, and their efficacy in the medicinal and pharmaceutical treatment of chronic diseases.

Adsorption Behavior and Dynamic Interactions of Anionic Acid Blue 25 on Agricultural Waste.

In this study, adsorption characteristics of a negatively charged dye, Acid Blue 25 (AB25), on pomelo pith (PP) was studied by varying the adsorption parameters, with the aim of evaluating the adsorption mechanism and establishing the role of hydrogen bonding interactions of AB25 on agricultural wastes. The kinetics, intraparticle diffusion, mechanism, and thermodynamics of the AB25 adsorption were systematically evaluated and analyzed by pseudo-first-order and pseudo-second-order kinetic models, the Weber-Morris intraparticle and Boyd mass transfer models, the Langmuir, Freundlich, Dubinin-Radushkevich, and Temkin isotherm models, and the Van't Hoff equation. It was found that AB25 adsorption followed pseudo-second-order kinetics, governed by a two-step pore-volume intraparticle diffusion of external mass transfer of AB25 onto the PP surface. The adsorption process occurred spontaneously. The adsorption mechanism could be explained by the Langmuir isotherm model, and the maximum adsorption capacity was estimated to be 26.9 mg g-1, which is comparable to many reported adsorbents derived from agricultural wastes. Changes in the vibrational spectra of the adsorbent before and after dye adsorption suggested that AB25 molecules are bound to the PP surface via electrostatic and hydrogen bonding interactions. The results demonstrated that the use of pomelo pith, similar to other agricultural wastes, would provide a basis to design a simple energy-saving, sustainable, and cost-effective approach to remove negatively charged synthetic dyes from wastewater.

Dispositional causes of burnout, satisfaction, and performance through the fear of COVID-19 during times of pandemic.

Rooted in research into personality, we propose that the Big Five traits would be related to fear of COVID-19 (FOC), which in turn would lead to heightened job burnout, reduced job satisfaction, and decreased performance. Utilizing a three-wave time-lagged design, we collected our data from employees working in the United States and Canada (N = 300 × 3). We found good support for our hypotheses. Extraversion, neuroticism, and conscientiousness had significant direct effects on FOC. Fear of COVID-19 was positively related to job burnout and negatively related to job satisfaction and performance. Extraversion, neuroticism, and conscientiousness had significant indirect effects on burnout, job satisfaction, and job performance via FOC. Hence, this study identifies a key mechanism, an individual's worry about losing their valuable resources (e.g. their health, and that of their family members and friends, etc.), through which selected dimensions of personality might affect employees' work outcomes. We discuss our findings and provide suggestions for future research in this domain.

Dynamics and thermodynamics for competitive adsorptive removal of methylene blue and rhodamine B from binary aqueous solution onto durian rind.

Concurrent adsorptive removal of methylene blue (MB) and rhodamine B (RhB) onto durian rind (DR) agricultural waste, from an aqueous binary solution as a model of wastewater containing multiple synthetic dyes, was investigated. The concurrent adsorption of the dyes followed pseudo-second-order kinetics. The adsorption isotherm was well simulated by the Langmuir model, implying a monolayer adsorption to the surface with a homogeneous binding energy. The adsorption process was governed by external mass transfer through two-step intraparticle diffusion of the dyes onto the adsorbent surface. The adsorption efficiency of MB (96.4%) is much higher than that of RhB (56.3%). This is attributed to the higher rate constant for the adsorption of MB (0.348 g mg-1 min-1) as compared to that of RhB (0.151 g mg-1 min-1). The adsorption behavior suggested that the two cationic dyes in the binary solution diffused and adsorbed independently and randomly onto the DR surface. The adsorption capacity of MB and RhB in the binary solution (47.4 mg g-1 and 32.9 mg g-1, respectively) is lower than those of their single solute solutions (93.3 mg g-1 and 62.8 mg g-1, respectively), suggesting a competitive effect in their concurrent adsorption. This was confirmed based on the adsorption characteristics of the binary solution with different molar ratios. The competitive effect was attributed to either non-interactive or repulsive electrostatic interactions between the positively charged dyes in the binary system. The domination of MB is attributed to its smaller molecular size, higher planarity, and faster adsorption kinetics compared with RhB.

Nanoparticle Assembling Dynamics Induced by Pulsed Optical Force.

Femtosecond (fs) laser trapping dynamics is summarized for silica, hydrophobically modified silica, and polystyrene nanoparticles (NPs) in aqueous solution, highlighting their distinct optical trapping dynamics under CW laser. Mutually repulsive silica nanoparticles are tightly confined under fs laser compared to CW laser trapping and, upon increasing laser power, they are ejected from the focus as an assembly. Hydrophobically modified silica and polystyrene (PS) NPs are sequentially ejected just like a stream or ablated, giving bubbles. The ejection and bubbling take place with the direction perpendicular to laser polarization and its direction is randomly switched from one to the other. These characteristic features are interpreted from the viewpoint of single assembly formation of NPs at an asymmetric position in the optical potential. Temporal change in optical forces map is prepared for a single PS NP by calculating scattering, gradient, and temporal forces. The relative contribution of the forces changes with the volume increase of the assembly and, when the pushing force along the trapping pulse propagation overcome the gradient in the focal plane, the assembly undergoes the ejection. Further fs multiphoton absorption is induced for the larger assembly leading to bubble generation. The assembling, ejection, and bubbling dynamics of NPs are characteristic features of pulsed optical force and are considered as a new platform for developing new material fabrication method.

Synergistic effect in concurrent removal of toxic methylene blue and acid red-1 dyes from aqueous solution by durian rind: kinetics, isotherm, thermodynamics, and mechanism.

In the present study, a synergistic effect between cationic methylene blue (MB) and anionic acid red 1 (AR1) on their concurrent adsorptive removal from aqueous binary solution onto durian rind (DR) was systematically investigated in batch mode across different parameters. The concurrent adsorption was pseudo-second-order kinetics and followed the Langmuir isotherm model, similar to their respective single component. The kinetics and intraparticle diffusion analyses demonstrated that the adsorption rate of MB was a 15-fold faster than AR1, and mass transports were governed by a combination of intraparticle and film diffusion. The synergistic effect was evidenced by an enhanced adsorption efficiency of AR1 from 27 to 42%, while that of MB was almost unchanged (97-98%). By changing the molar ratios of MB and AR1, it was found that the maximum adsorption capacity of MB and AR1 was 249 and 200 mg g-1, respectively, in the binary system higher compared with those in their respective single system (108 and 16 mg g-1). Overall data indicated that the synergistic effect was due to electrostatic interactions between cationic and anionic synthetic dyes, supported by negatively charged DR surface, leading to the formation of their stacking layers on the adsorbent surface. Novelty statement: A synergistic effect in concurrent adsorptive removal of synthetic dyes from multicomponent wastewater remains a critical research challenge. We believed that electrostatic interaction between ionic dyes could be explored to enhance their removal efficiency. This report is the first time that such a synergistic effect between cationic methylene blue (MB) and anionic acid red 1 (AR1) on their concurrent adsorption from aqueous binary system is systematically investigated. The kinetics, isotherm, thermodynamics, and mechanism of the concurrent adsorption of MB and AR1 attributed to the synergistic effect are elucidated in detail.

Simultaneous adsorption of lanthanum and yttrium from aqueous solution by durian rind biosorbent.

This paper presents the adsorption capacity of a biosorbent derived from the inner part of durian (Durio zibethinus) rinds, which are a low-cost and abundant agro-waste material. The durian rind sorbent has been successfully utilized to remove lanthanum (La) and yttrium (Y) ions from their binary aqueous solution. The effects of several adsorption parameters including contact time, pH, concentrations of La and Y, and temperature on the removal of La and Y ions were investigated. The adsorption isotherm and kinetics of the metal ions were also evaluated in detail. Both La and Y ions were efficiently adsorbed by the biosorbent with optimum adsorption capacity as high as 71 mg La and 35 mg Y per gram biosorbent, respectively. The simultaneous adsorption of La and Y ions follows Langmuir isotherm model, due to the favorable chelation and strong chemical interactions between the functional groups on the surface of the biosorbent and the metal ions. The addition of oxygen content after adsorption offers an interpretation that the rare-earth metal ions are chelated and incorporated most probably in the form of metal oxides. With such high adsorption capacity of La and Y ions, the durian rind sorbent could potentially be used to treat contaminated wastewater containing La and Y metal ions, as well as for separating and extracting rare-earth metal ions from crude minerals.

A layer-by-layer ZnO nanoparticle-PbS quantum dot self-assembly platform for ultrafast interfacial electron injection.

Absorbent layers of semiconductor quantum dots (QDs) are now used as material platforms for low-cost, high-performance solar cells. The semiconductor metal oxide nanoparticles as an acceptor layer have become an integral part of the next generation solar cell. To achieve sufficient electron transfer and subsequently high conversion efficiency in these solar cells, however, energy-level alignment and interfacial contact between the donor and the acceptor units are needed. Here, the layer-by-layer (LbL) technique is used to assemble ZnO nanoparticles (NPs), providing adequate PbS QD uptake to achieve greater interfacial contact compared with traditional sputtering methods. Electron injection at the PbS QD and ZnO NP interface is investigated using broadband transient absorption spectroscopy with 120 femtosecond temporal resolution. The results indicate that electron injection from photoexcited PbS QDs to ZnO NPs occurs on a time scale of a few hundred femtoseconds. This observation is supported by the interfacial electronic-energy alignment between the donor and acceptor moieties. Finally, due to the combination of large interfacial contact and ultrafast electron injection, this proposed platform of assembled thin films holds promise for a variety of solar cell architectures and other settings that principally rely on interfacial contact, such as photocatalysis.

Real-time observation of ultrafast electron injection at graphene-Zn porphyrin interfaces.

We report on the ultrafast interfacial electron transfer (ET) between zinc(II) porphyrin (ZnTMPyP) and negatively charged graphene carboxylate (GC) using state-of-the-art femtosecond laser spectroscopy with broadband capabilities. The steady-state interaction between GC and ZnTMPyP results in a red-shifted absorption spectrum, providing a clear indication for the binding affinity between ZnTMPyP and GC via electrostatic and π-π stacking interactions. Ultrafast transient absorption (TA) spectra in the absence and presence of three different GC concentrations reveal (i) the ultrafast formation of singlet excited ZnTMPyP*, which partially relaxes into a long-lived triplet state, and (ii) ET from the singlet excited ZnTMPyP* to GC, forming ZnTMPyP˙(+) and GC˙(-), as indicated by a spectral feature at 650-750 nm, which is attributed to a ZnTMPyP radical cation resulting from the ET process.

Optical trapping of nanoparticles by ultrashort laser pulses.

Optical trapping with continuous-wave lasers has been a fascinating field in the optical manipulation. It has become a powerful tool for manipulating micrometer-sized objects, and has been widely applied in physics, chemistry, biology, material, and colloidal science. Replacing the continuous-wave- with pulsed-mode laser in optical trapping has already revealed some novel phenomena, including the stable trap, modifiable trapping positions, and controllable directional optical ejections of particles in nanometer scales. Due to two distinctive features; impulsive peak powers and relaxation time between consecutive pulses, the optical trapping with the laser pulses has been demonstrated to have some advantages over conventional continuous-wave lasers, particularly when the particles are within Rayleigh approximation. This would open unprecedented opportunities in both fundamental science and application. This Review summarizes recent advances in the optical trapping with laser pulses and discusses the electromagnetic formulations and physical interpretations of the new phenomena. Its aim is rather to show how beautiful and promising this field will be, and to encourage the in-depth study of this field.

Energy balance and life cycle assessments in producing microalgae biodiesel via a continuous microalgal-bacterial photobioreactor loaded with wastewater.

Life cycle assessments of microalgal cultivation systems are often conducted to evaluate the sustainability and feasibility factors of the entire production chain. Unlike widely reported conventional microalgal cultivation systems, the present work adopted a microalgal-bacterial cultivation approach which was upscaled into a pilot-scale continuous photobioreactor for microalgal biomass production into biodiesel from wastewater resources. A multiple cradle-to-cradle system ranging from microalgal biomass-to-lipid-to-biodiesel was evaluated to provide insights into the energy demand of each processes making up the microalgae-to-biodiesel value chain system. Energy feasibility studies revealed positive NER values (4.95-8.38) for producing microalgal biomass but deficit values for microalgal-to-biodiesel (0.14-0.23), stemming from the high energy input requirements in the downstream processes for converting biomass into lipid and biodiesel accounting to 88-90% of the cumulative energy demand. Although the energy balance for microalgae-to-biodiesel is in the deficits, it is comparable with other reported biodiesel production case studies (0.12-0.40). Nevertheless, the approach to using microalgal-bacterial cultivation system has improved the overall energy efficiency especially in the upstream processes compared to conventional microalgal cultivation systems. Energy life cycle assessments with other microalgal based biofuel systems also proposed effective measures in increasing the energy feasibility either by utilizing the residual biomass and less energy demanding downstream extraction processes from microalgal biomass. The microalgal-bacterial cultivation system is anticipated to offer both environmental and economic prospects for upscaling by effectively exploiting the low-cost nutrients from wastewaters via bioconversion into valuable microalgal biomass and biodiesel.

Assessing Microalgal Protein's Impact on Environment and Energy Footprint via Life Cycle Analysis.

Conventionally, increasing the yield of microalgal biomass has been the primary focus of research, while the significant protein reserve within this biomass has remained largely unexplored. This protein reserve possesses substantial value and versatility, offering a wide range of prospective applications and presenting an enticing chance for innovation and value enhancement for various sectors. Current study employed an innovative research approach that focused solely on the LCA of protein production potential from microalgal biomass, a lesser-explored aspects within this domain. Most environmental impact categories were shown to be significantly affected by cultivation phase because of the electrical obligation, followed by the harvesting and protein extraction phase. Still, the environmental aspect was seen to yield a minimal impact on global warming potential, i.e., 4 × 10-3 kg CO2, underscoring the ecologically favorable nature of the process. Conversely, the overall energy impact was seen to intensify with NEB of - 39.33 MJ and NER of 0.49, drawing attention to the importance of addressing the energy aspect to harness the full potential of microalgal protein production.

Solid-state mechanochemical synthesis of chitosan from mud crab (Scylla serrata) chitin.

This study presents a method for solvent-free mechanochemical synthesis of chitosan from chitin, sourced from the shells of mud crabs (Scylla serrata). The procedure involves a sequence of demineralization and deproteinization to extract chitin from the crab shells, followed by mechanochemical deacetylation. The chitin was deacetylated by grinding it as a solid blend with sodium hydroxide (NaOH) using a stainless steel mortar and pestle. After grinding, chitosan is isolated from the blend by repetitive washing and centrifugation. The chitosan product is then characterized using Fourier transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction analysis. These characterization techniques confirm the successful deacetylation of chitin to form chitosan. A high degree of deacetylation (DD) is achieved when the weight ratio of NaOH to chitin is 1:1 or higher, implying that the DD value can be enhanced by increasing this weight ratio. The mechanochemical reaction mechanism involves the hydroxyl groups on the NaOH particles reacting with the acetamide groups of the chitin strands, yielding solid chitosan and sodium acetate. This mechanochemical deacetylation approach is more practical than the conventional heterogeneous deacetylation in strong basic solutions, since it could suppress depolymerization of the resulting chitosan and requires significantly less base. This makes it a promising method for large-scale industrial applications.

Optimization of Biocomposite Film Based on Whey Protein Isolate and Nanocrystalline Cellulose from Pineapple Crown Leaf Using Response Surface Methodology.

This study employed response surface methodology to optimize the preparation of biocomposites based on whey protein isolate, glycerol, and nanocrystalline cellulose from pineapple crown leaf. The effects of different concentrations of nanocrystalline cellulose as a filler and glycerol as a plasticizer on the thickness, the tensile strength, and the elongation at break on the resulting biocomposite films were investigated. The central composite design was used to determine the optimum preparation conditions for biocomposite films with optimum properties. The regression of a second-order polynomial model resulted in an optimum composition consisting of 4% glycerol and 3.5% nanocrystalline cellulose concentrations, which showed a desirability of 92.7%. The prediction of the regression model was validated by characterizing the biocomposite film prepared based on the optimum composition, at which the thickness, tensile strength, and elongation at break of the biocomposite film were 0.13 mm, 7.16 MPa, and 39.10%, respectively. This optimum composition can be obtained in range concentrations of glycerol (4-8%) and nanocrystalline cellulose (3-7%). Scanning electron microscope images showed that nanocrystalline cellulose dispersed well in the pure whey protein isolate, and the films had a relatively smooth surface. In comparison, a rough and uneven surface results in more porous biocomposite films. Fourier transform infrared spectroscopy revealed that nanocrystalline cellulose and glycerol showed good compatibility with WPI film by forming hydrogen bonds. The addition of nanocrystalline cellulose as a filler also decreased the transparency, solubility, and water vapor permeability and increased the crystallinity index of the resulting biocomposite film.

Antimicrobial activity of silver sulfide quantum dots functionalized with highly conjugated Schiff bases in a one-step synthesis.

In the present paper, low-dimensional Ag2S QDs were fabricated for the first time, with four different dithiocarbazate derivative Schiff bases (SB) as capping agents in a one-pot synthesis. These SB-capped Ag2S QDs were almost spherical with an average size range of 4.0 to 5.6 nm, which is slightly smaller than conventional thioglycolic acid (TGA)-capped Ag2S QDs. We demonstrate that the growth of Gram-positive bacteria (Bacillus subtillus and Staphylococcus aureus), Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa), and a prevalent fungal pathogen (Candida albicans) are inhibited more when the bacterial and fungal cells were nurtured with the synthesized SB-Ag2S QDs, compared with TGA-Ag2S QDs or free unbound Schiff bases. The minimum inhibitory concentration (MIC) results confirmed that even low concentrations of SB-Ag2S QDs were able to inhibit bacterial (MIC 5-75 µg mL-1) and fungal growth (MIC 80-310 µg mL-1), and in some cases they performed better than streptomycin (8-25 µg mL-1). Lethality bioassay results confirmed that SB-Ag2S QDs were not toxic to brine shrimp (Artemia salina). The results show that capping agents are essential in the design of functional Ag2S QDs, and highlight that Schiff bases provide an excellent opportunity to optimize the biological activities of silver based QDs.

Degradation of ciprofloxacin in aqueous solution using ozone microbubbles: spectroscopic, kinetics, and antibacterial analysis.

Ciprofloxacin (CIP) has been listed in the last version of the surface water due to its ability to kill human cells by inhibiting the activity of DNA topoisomerase IV. Thus, CIP, along with other antibiotic pollution has become a serious threat to the environment and public health. Ozonation has been used as an advanced technique that is applied in wastewater treatment to remove CIP, but the primary limitation of this method is the low solubility of ozone in water. This study is the first report of CIP removal in a scale-up of its aqueous solution using a self-developed aerator pump-enhanced ozonation (APO) system, which only employs a propeller and a zigzag arrangement of meshes. This aerator pump decreased the size of ozone bubbles by 90% and increased the effective ozone solubility to 0.47 ppm. The mechanism of degradation of CIP is attributed to an oxidation reaction of the antibiotic with reactive oxygen species, such as hydroxyl, oxygen, and hydroperoxyl radicals, generated on the surface of the ozone microbubbles. It was found that the rate and efficiency of degradation of CIP using the APO system were 3.64 × 10-3/min and 83.5%, respectively, which is higher compared with those of conventional flow ozonation (FO) systems (1.47 × 10-3/min and 60.9%). The higher degradation efficiency of CIP by the APO system was also revealed by its higher electrical energy efficiency (0.146 g/kWh), compared to that of the FO system (0.106 g/kWh). The degradation of CIP was also monitored by the resulting antibacterial activity against Escherichia coli and Staphylococcus aureus.

Compaction of a Polymeric Membrane in Ultra-Low-Pressure Water Filtration.

Applications of ultra-low-pressure filtration systems are increasing as they offer enhanced sustainability due to lower energy input, almost no use of chemicals, and minimum operational expenditure. In many cases, they operate as a decentralized system using a gravity-driven membrane (GDM) filtration process. These applications are relatively new; hence, the fundamental knowledge of the process is still limited. In this study, we investigated the phenomenon of polymeric membrane compaction under an ultra-low-pressure system. The compaction phenomenon is well-recognized in the traditional pressure-driven system operating at high transmembrane pressures (ΔPs > 200 kPa), but it is less documented in ultra-low-pressure systems (ΔP < 10 kPa). A simple GDM filtration setup operated under a constant-pressure system was employed to investigate the compaction phenomena in a polymeric hollow fiber membrane for clean water filtration. Firstly, a short-term pressure stepping test was performed to investigate the occurrence of instantaneous compaction in the ΔP range of 1−10 kPa. The slow compaction was later investigated. Finally, the compaction dynamic was assessed under alternating high and low ΔP and relaxation in between the filtrations. The findings demonstrated the prominence of membrane compaction, as shown by the decreasing trend in clean water permeability at higher ΔPs (i.e., 3240 and 2401 L m−2 h−1 bar−1 at ΔPs of 1 and 10 kPa, respectively). We also found that the intrinsic permeability of the applied polymeric membrane was significantly higher than the apparent one (4351 vs. 2401 L m−2 h−1 bar−1), demonstrating >50% loss due to compaction. The compaction was mainly instantaneous, which occurred when the ΔP was changed, whereas only minor changes in permeability occurred over time when operating at a constant ΔP. The compaction was highly reversible and could be restored (i.e., decompaction) through relaxation by temporarily stopping the filtration. A small fraction of irreversible compaction could be detected by operating alternating filtrations under ΔPs of 1 and 10 kPa. The overall findings are essential to support emerging GDM filtration applications, in which membrane compaction has been ignored and confounded with membrane fouling. The role of compaction is more prominent for high-flux GDM filtration systems treating less-fouling-prone feed (i.e., rainwater, river water) and involving membrane cleaning (i.e., relaxation) in which both reversible and irreversible compaction occurred simultaneously.

New insights into the ultrafast photophysics of oxidized and reduced FAD in solution.

The ultrafast photophysics of oxidized and reduced flavin adenine dinucleotide (FAD) in aqueous solution was studied by broadband UV-vis femtosecond transient absorption spectroscopy. We observed that oxidized FAD (FAD(ox)) in solution readily aggregates at submillimolar concentration. Upon excitation of FAD(ox), three excited-state lifetimes were found and assigned to three different species: the closed (stacked) conformation of the monomer (∼5.4 ps), the open (extended) conformation of the monomer (∼2.8 ns), and the dimer (∼27 ps). In the case of the stacked conformation of the monomer, we show that intramolecular electron transfer from the adenine to the isoalloxazine ring occurs with a time constant of 5.4 ps and is followed by charge recombination on a faster time scale, namely, 390 fs. We additionally demonstrate that deprotonated reduced flavin (FADH(-)) undergoes biphotonic ionization under high excitation fluence and dissociates into a hydrated electron and the neutral semiquinone radical FADH(•).

Biocompatible chitin-encapsulated CdS quantum dots: Fabrication and antibacterial screening.

Chitin-encapsulated cadmium sulfide quantum dots (CdS@CTN QDs) were successfully synthesized from chitin and Cd(NO3)2 precursor using the colloidal chemistry method, toward the development of biocompatible and biodegradable QDs for biomedical applications. CdS@CTN QDs exhibited the nanocrystalline cubic CdS encapsulated by α-chitin. The average particle size of CdS@CTN QDs was estimated using empirical Henglein model to be 3.9 nm, while their crystallite size was predicted using Scherrer equation to be 4.3 nm, slightly larger compared to 3-mercaptopropionic acid-capped CdS QDs (3.2 and 3.6 nm, respectively). The mechanism of formation was interpreted based on the spectroscopic data and X-ray crystal structures of CdS@CTN QDs fabricated at different pH values and mass ratios of chitin to Cd(NO3)2 precursor. As an important step to explore potential biomolecular and biological applications of CdS@CTN QDs, their antibacterial activities were tested against four different bacterial strains; i.e. Escherichia coli, Bacillus subtillus, Staphylococcus aureus and Pseudomonas aeruginosa.

Design, synthesis and antiamoebic activity of dysprosium-based nanoparticles using contact lenses as carriers against Acanthamoeba sp.

PURPOSE: Contact lenses have direct contact with the corneal surface and can induce sight-threatening infection of the cornea known as Acanthamoeba keratitis. The objective of this study was to evaluate the dysprosium-based nanoparticles (Dy-based NPs), namely Fe3 O4 -PEG-Dy2 O3 nanocomposites and Dy(OH)3 nanorods, as an active component against Acanthamoeba sp., as well as the possibility of their loading onto contact lenses as the drug administering vehicle to treat Acanthamoeba keratitis (AK). METHODS: The Dy-based NPs were synthesized, and they were loaded onto commercial contact lenses. The loading content of the NPs and their release kinetics was determined based on the absorbance of their colloidal solution before and after soaking the contact lenses. The cytotoxicity of the NPs was evaluated, and the IC50 values of their antiamoebic activity against Acanthamoeba sp. were determined by MTT colorimetric assay, followed by observation on the morphological changes by using light microscopy. The mechanism of action of the Dy-based NPs against Acanthamoeba sp. was evaluated by DNA laddering assays. RESULTS: The loading efficiencies of the Dy-based NPs onto the contact lens were in the range of 30.6-36.1% with respect to their initial concentration (0.5 mg ml-1 ). The Dy NPs were released with the flux approximately 5.5-11 µg cm-2  hr-1 , and the release was completed within 10 hr. The emission of the NPs consistently showed a peak at 575 nm due to Dy3+ ion, offering the possible monitoring and tracking of the NPs. The SEM images indicated the NPs are aggregated on the surface of the contact lenses. The DNA ladder assay suggested that the cells underwent DNA fragmentation, and the cell death was due most probably to necrosis, rather than apoptosis. The cytotoxicity assay of Acanthamoeba sp. suggested that Fe3 O4 -PEG, Fe3 O4 -PEG-Dy2 O3 , Dy(NO3 )3 .6H2 O and Dy(OH)3 NPs have an antiamoebic activity with the IC50 value being 4.5, 5.0, 9.5 and 22.5 µg ml-1 , respectively. CONCLUSIONS: Overall findings in this study suggested that the Dy-based NPs can be considered as active antiamoebic agents and possess the potential as drugs against Acanthamoeba sp. The NPs could be loaded onto the contact lenses; thus, they can be potentially utilized to treat Acanthamoeba keratitis (AK).