RESUMEN
It is accepted that cancer stem cells (CSCs) are key to the occurrence, progression, drug resistance, and recurrence of bladder cancer (BLCA). Here, we aimed to characterize the landscapes of CSCs and investigate the biological and clinical signatures based on a prognostic model constructed by genes associated with CSCs. The malignant epithelial cells were discovered and sorted into six clusters through single cell analysis. C2 was identified as the CSCs. The signaling involved in the interactions between C2, cancer-associated fibroblasts (CAFs), and immune cells mainly consisted of MK, THBS, ANGPTL, VISFATIN, JAM, and ncWNT pathways. The CSC-like prognostic index (CSCLPI) constructed by the random survival forest was a reliable risk factor for BLCA and had a stable and powerful effect on predicting the overall survival of patients with BLCA. The level of CAFs was higher among patients with higher CSCLPI scores, suggesting that CAFs play a significant role in regulating biological characteristics. The CSCLPI-developed survival prediction nomogram has the potential to be applied clinically to predict the 1-, 2-, 3-, and 5-year overall survival of patients with BLCA. The CSCLPI can be used for prognostic prediction and drug treatment evaluation in the clinic.
RESUMEN
Clostera anachoreta is one of the important Lepidoptera insect pests in forestry, especially in poplars woods in China, Europe, Japan, and India, and so forth, and also the target insect of Cry1Ac toxin and Bt plants. Six genes, HSC70, GNB2L/RACK1, PNLIP, BI1-like, arylphorin type 2, and PKM were found in this study, and they might be associated with the response to the Cry1Ac toxin, found by analyzing the transcriptome data. And the PI3K-Akt pathway was highly enriched in differentially expressed unigenes and linked to several crucial pathways, including the B-cell receptor signaling pathway, toll-like receptor pathway, and mitogen-activated protein kinase signaling pathway. They might be involved in the recovery stage of the damaged midgut during the response to sublethal doses of Cry1Ac toxin. This is the first study conducted to specifically investigate C. anachoreta response to Cry toxin stress using large-scale sequencing technologies, and the results highlighted some important genes and pathways that could be involved in Btcry1Ac resistance development or could serve as targets for biologically based control mechanisms of this insect pest.